transformaid tm bacterial transformation kit  (Thermo Fisher)


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    Name:
    TransformAid Bacterial Transformation Kit
    Description:
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    Catalog Number:
    k2710
    Price:
    None
    Applications:
    Cloning|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher transformaid tm bacterial transformation kit
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    https://www.bioz.com/result/transformaid tm bacterial transformation kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    transformaid tm bacterial transformation kit - by Bioz Stars, 2020-02
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    Related Articles

    Clone Assay:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Recombinant colonies were selected, and the plasmids were extracted according to Sambrook et al. [ ].

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof Modification of the phage binding motif was achieved by standard cloning procedures. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Article Title: A connecting hinge represses the activity of endothelial nitric oxide synthase
    Article Snippet: Restriction digestions, cloning, and bacterial growth were performed using standard procedures. .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: The mIL-12 expression cassette was cloned into the pCRBluntpsiCat plasmid using NotI , SwaI , and PmlI restriction enzymes, and the antibiotic resistance-free plasmid was produced using the X-mark™ technology and antibiotic-free maintenance system ORT® (Cobra Biologics). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Restriction digestions, cloning, and bacterial growth were performed using standard procedures. .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD).

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    Amplification:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Following amplification, phage DNA was extracted using the E.Z.N.A. plasmid miniprep kit I (peqLab, Erlangen, Germany) and inserts were verified by DNA sequencing.

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The OligoA primer yielded an amplicon the size of the insert, while the pair OligoA/Oligo CA yielded a second amplicon from the clones containing the CA repeat motif.

    Whole Genome Amplification:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: PCR was carried out in a 25 µl reaction volume containing 1 µl of diluted WGA product, 12.5 µl of 2× Xtreme Buffer, 500 µM (each) of dNTPs, 0.02 U of KOD Xtreme™ Hot Start DNA Polymerase, and 0.6 pmol of each primer. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    MTT Assay:

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: Materials Branched PEI (LMW PEI; average MW = 1,800 Da), succinic anhydride, MTT, N-hydroxybenzotriazole, 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride, 1-hydroxybenzotriazole hydrate, and N-[2-hydroxythyl] piperazine-N-[2-ethanesulfonic acid] (HEPES) were purchased from Sigma-Aldrich (Munich, Germany). .. Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA).

    Construct:

    Article Title: A connecting hinge represses the activity of endothelial nitric oxide synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Oligonucleotides used to construct site-directed mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: To construct pORF-mIL-12-ORT, standard cloning methods and operator-repressor titration (ORT) technology [ , ] were used, followed by transformation into competent (E. coli ) cells ( ). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD). .. Oligonucleotides used to construct mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Real-time Polymerase Chain Reaction:

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA). .. Plasmid purification was performed by Qiagen Endofree Mega Plasmid Kit (Qiagen, Hilden, Germany). cDNA synthesis and Real Time PCR were performed using the PrimeScript™ RT reagent Kit (Perfect Real Time, TaKaRa, Dalian, People’s Republic of China), and RealQ Plus 2x Master Mix Green High ROX™ (AmpliQon, Denmark).

    Incubation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Expressing:

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: .. Bacterial transformation The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: The mIL-12 expression cassette was cloned into the pCRBluntpsiCat plasmid using NotI , SwaI , and PmlI restriction enzymes, and the antibiotic resistance-free plasmid was produced using the X-mark™ technology and antibiotic-free maintenance system ORT® (Cobra Biologics). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Paragraph title: Preparation of nNOS expression plasmids ... Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD).

    Modification:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof Modification of the phage binding motif was achieved by standard cloning procedures. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Wildtype and mutant rat nNOS proteins containing a His6 tag attached to their N termini were overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD).

    Transformation Assay:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: Paragraph title: Transformation of sodC Cloned pTZ57R into E. coli DH5α ... The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: Bacterial transformation The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: .. Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Rat CaM plasmid was a gift from Dr. Anthony Persechini.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: To construct pORF-mIL-12-ORT, standard cloning methods and operator-repressor titration (ORT) technology [ , ] were used, followed by transformation into competent (E. coli ) cells ( ). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    Article Title: Influence of Heme-Thiolate in Shaping the Catalytic Properties of a Bacterial Nitric-oxide Synthase *
    Article Snippet: .. Mutated plasmids (pET15b-BsNOS-W66H and pET15b-BsNOS-W66F) were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Wild-type and mutant BsNOS proteins containing a His6 tag attached to their N termini were overexpressed in E. coli strain BL21(DE3).

    Sequencing:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Paragraph title: Cloning and sequencing ... Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Southern Blot:

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Ligation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Cell Culture:

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: Cell culture experiments were performed using FBS and DMEM (Gibco, Gaithersburg, MD, USA). .. Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA).

    DNA Sequencing:

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Nucleotide sequences were obtained from an ABI PRISM 3130 XL Genetic Analyzer (Hitachi, Tokyo, Japan) by the Facility of DNA Sequencing of the Chemistry Institute of the University of São Paulo and were deposited in the GenBank under accession numbers MH571141–MH571149.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Following amplification, phage DNA was extracted using the E.Z.N.A. plasmid miniprep kit I (peqLab, Erlangen, Germany) and inserts were verified by DNA sequencing.

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: Mutations were confirmed by DNA sequencing at the Cleveland Clinic Genomics Core Facility. .. Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD). .. All mutated constructs were confirmed by DNA sequencing at the Cleveland Clinic Genomics Core.

    Electroporation Bacterial Transformation:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Recombinant colonies were selected, and the plasmids were extracted according to Sambrook et al. [ ].

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: .. Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA). .. Plasmid purification was performed by Qiagen Endofree Mega Plasmid Kit (Qiagen, Hilden, Germany). cDNA synthesis and Real Time PCR were performed using the PrimeScript™ RT reagent Kit (Perfect Real Time, TaKaRa, Dalian, People’s Republic of China), and RealQ Plus 2x Master Mix Green High ROX™ (AmpliQon, Denmark).

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: .. Bacterial transformation The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: A connecting hinge represses the activity of endothelial nitric oxide synthase
    Article Snippet: .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Oligonucleotides used to construct site-directed mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: .. Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Rat CaM plasmid was a gift from Dr. Anthony Persechini.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD). .. Oligonucleotides used to construct mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    Article Title: Influence of Heme-Thiolate in Shaping the Catalytic Properties of a Bacterial Nitric-oxide Synthase *
    Article Snippet: .. Mutated plasmids (pET15b-BsNOS-W66H and pET15b-BsNOS-W66F) were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Wild-type and mutant BsNOS proteins containing a His6 tag attached to their N termini were overexpressed in E. coli strain BL21(DE3).

    Recombinant:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Recombinant colonies were selected, and the plasmids were extracted according to Sambrook et al. [ ].

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    DNA Extraction:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Molecular Cloning:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Magnetic Beads:

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: Basically, the Dynabeads® M-270 Streptavidin coated magnetic beads (Dynal Invitrogen USA) were coupled with the biotinylated probe, then this complex was hybridized with the microsatellite DNA containing fragments which were fished out from the reaction mix with a magnet. .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin.

    Mutagenesis:

    Article Title: A connecting hinge represses the activity of endothelial nitric oxide synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Site-directed mutagenesis was performed using a QuikChange XL site-directed mutagenesis kit (Stratagene, La Jolla, CA).

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: Paragraph title: Site-directed Mutagenesis ... Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Wildtype and mutant rat nNOS proteins containing a His6 tag attached to their N termini were overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD).

    Article Title: Influence of Heme-Thiolate in Shaping the Catalytic Properties of a Bacterial Nitric-oxide Synthase *
    Article Snippet: Paragraph title: Site-directed Mutagenesis ... Mutated plasmids (pET15b-BsNOS-W66H and pET15b-BsNOS-W66F) were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Isolation:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization To obtain PcP190 satDNA probes, PcP190 sequences were isolated from the genomic DNA of Pseudis fusca . .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: PcP190 satDNA was isolated by PCR using the primers P190F (5′-AGACTGGCTGGGAATCCCAG-3′) and P190R (5′-AGCTGCTGCGATCTGACAAGG-3′), described previously by . .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. All plasmids were isolated and purified using an EndoFree Plasmid Mega Kit (Qiagen, Hilden, Germany) according to the instructions provided with the kit.

    Purification:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA). .. Plasmid purification was performed by Qiagen Endofree Mega Plasmid Kit (Qiagen, Hilden, Germany). cDNA synthesis and Real Time PCR were performed using the PrimeScript™ RT reagent Kit (Perfect Real Time, TaKaRa, Dalian, People’s Republic of China), and RealQ Plus 2x Master Mix Green High ROX™ (AmpliQon, Denmark).

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. All plasmids were isolated and purified using an EndoFree Plasmid Mega Kit (Qiagen, Hilden, Germany) according to the instructions provided with the kit.

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    Polymerase Chain Reaction:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    IA:

    Article Title: A connecting hinge represses the activity of endothelial nitric oxide synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Oligonucleotides used to construct site-directed mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: Oligonucleotides for site-directed mutagenesis were obtained from Integrated DNA Technologies (Coralville, IA) and are listed in . .. Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD). .. Oligonucleotides used to construct mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Titration:

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: To construct pORF-mIL-12-ORT, standard cloning methods and operator-repressor titration (ORT) technology [ , ] were used, followed by transformation into competent (E. coli ) cells ( ). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    In Situ Hybridization:

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Recombinant colonies were selected, and the plasmids were extracted according to Sambrook et al. [ ].

    Article Title: Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene
    Article Snippet: Human CD200 plasmid (pCMV-XL5-hCD200) was obtained from OriGene (Rockville, MD, USA). .. Transfor-mAid Bacterial Transformation Kit (K-2710) was purchased from Thermo Scientific Company (Hanover, MD, USA).

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Following amplification, phage DNA was extracted using the E.Z.N.A. plasmid miniprep kit I (peqLab, Erlangen, Germany) and inserts were verified by DNA sequencing.

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: .. Bacterial transformation The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: Rat nNOS mutants were prepared by site-directed mutagenesis on a pCWori vector containing the cDNA of wild-type rat nNOS ( ). .. Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Article Title: A cross-domain charge interaction governs the activity of NO synthase
    Article Snippet: Wildtype and mutant rat nNOS proteins containing a His6 tag attached to their N termini were overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas-Thermo Scientific, Hanover, MD).

    Article Title: Isolation and Characterization of the First Microsatellite Markers for the Endangered Relict Mussel Hypanis colorata (Mollusca: Bivalvia: Cardiidae)
    Article Snippet: .. The PCR products were purified using the Gene JET™ PCR Purification Kit (Fermentas UAB, Vilnius, Lithuania), ligated into the pJET1.2 vector using the CloneJET™ PCR Cloning Kit (Fermentas UAB, Vilnius, Lithuania), transformed into DH5α Escherichia coli competent cells, using the TransformAid™ Bacterial Transformation Kit (Fermentas UAB, Vilnius, Lithuania) and plated on LB agar plates containing 50 μg/μL ampicillin. .. The pJET1.2 vector used for cloning contains a lethal gene which is disrupted by the successful insertion of the cloned DNA fragment and, as a consequence, only the colonies containing DNA inserts grew on the agar plates.

    Article Title: Influence of Heme-Thiolate in Shaping the Catalytic Properties of a Bacterial Nitric-oxide Synthase *
    Article Snippet: BsNOS mutants were prepared by site-directed mutagenesis on a pET15b vector containing the cDNA of wild-type BsNOS ( ). .. Mutated plasmids (pET15b-BsNOS-W66H and pET15b-BsNOS-W66F) were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Electrophoresis:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Binding Assay:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Agarose Gel Electrophoresis:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Chromosome Walking:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Sequencing by primer walking was carried out for both clones, confirming the full genome sequence of HPV-150.

    Spectrophotometry:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Produced:

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: The mIL-12 expression cassette was cloned into the pCRBluntpsiCat plasmid using NotI , SwaI , and PmlI restriction enzymes, and the antibiotic resistance-free plasmid was produced using the X-mark™ technology and antibiotic-free maintenance system ORT® (Cobra Biologics). .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Gel Extraction:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Article Snippet: .. The restriction enzymes, Ligation Kit, Gel Extraction Kit, Plasmid Miniprep Kit, and TransformAid Bacterial Transformation Kit together with the E. coli strain JM107 were all purchased from Thermo Fisher Scientific (Waltham, MA, USA). ..

    Chick Chorioallantoic Membrane Assay:

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: .. Bacterial transformation The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: A Bridging Interaction Allows Calmodulin to Activate NO Synthase through a Bi-modal Mechanism *
    Article Snippet: Mutated plasmids were transformed into E. coli BL21(DE3) cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Rat CaM plasmid was a gift from Dr. Anthony Persechini.

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  • 90
    Thermo Fisher transformaid bacterial transformation kit
    Transformaid Bacterial Transformation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
    Average 90 stars, based on 30 article reviews
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    transformaid bacterial transformation kit - by Bioz Stars, 2020-02
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