transformaid kit  (Thermo Fisher)


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    Name:
    TransformAid Bacterial Transformation Kit
    Description:

    Catalog Number:
    K2711
    Price:
    None
    Score:
    85
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    Structured Review

    Thermo Fisher transformaid kit

    https://www.bioz.com/result/transformaid kit/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    transformaid kit - by Bioz Stars, 2019-12
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: Paragraph title: Cloning and expression of EqCXCL16 in Escherichia coli . ... The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Centrifugation:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Amplification:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The amplified EqCXCL16 fragment (amino acids [aa] 17 to 247) was run on a 1% agarose gel (E-Gel EX; Life Technologies, Grand Island, NY) and purified with a commercial kit (Zymoclean gel recovery kit; Zymo Research, Irvine, CA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Recovery:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The amplified EqCXCL16 fragment (amino acids [aa] 17 to 247) was run on a 1% agarose gel (E-Gel EX; Life Technologies, Grand Island, NY) and purified with a commercial kit (Zymoclean gel recovery kit; Zymo Research, Irvine, CA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    DNA Ligation:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The purified EqCXCL16 fragment was digested with the XhoI and BamHI restriction enzymes and ligated (Rapid DNA ligation kit; Thermo Scientific, Rockford, IL) into the bacterial expression vector pET15b (EMD Millipore Novagen, Temecula, CA) following digestion with the same restriction enzymes. .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Construct:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The purified EqCXCL16 fragment was digested with the XhoI and BamHI restriction enzymes and ligated (Rapid DNA ligation kit; Thermo Scientific, Rockford, IL) into the bacterial expression vector pET15b (EMD Millipore Novagen, Temecula, CA) following digestion with the same restriction enzymes. .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. Recombinant plasmid p15-16A (aa 17 to 247) was isolated from ampicillin-resistant clones using a ZR plasmid miniprep kit (Zymo Research, Irvine, CA).

    Electrophoresis:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas).

    Expressing:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: Paragraph title: Cloning and expression of EqCXCL16 in Escherichia coli . ... The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Article Title: Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast
    Article Snippet: Paragraph title: Construction of E.coli strains expressing divIVA–gfp fusions ... For the minB mutant P678-54 and cold-sensitive FtsZ strain PB143(pDB346), competent cells were prepared using the TransformAid kit (MBI fermentas).

    Transformation Assay:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. Recombinant plasmid p15-16A (aa 17 to 247) was isolated from ampicillin-resistant clones using a ZR plasmid miniprep kit (Zymo Research, Irvine, CA).

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: The products were analyzed by gel electrophoresis through a 1.2% agarose gel in Tris-acetate-EDTA. .. Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Cultures were grown to log phase in Luria broth (LB) plus 15 μg of tetracycline (Sigma)/ml and treated with the indicated concentrations of novobiocin (Sigma) for 20 min with shaking.

    Article Title: Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast
    Article Snippet: Escherichia coli strains were transformed with pSG1044 ( divIVA–gfpS65T ) and selected on ampicillin plates (50 µg/ml). .. For the minB mutant P678-54 and cold-sensitive FtsZ strain PB143(pDB346), competent cells were prepared using the TransformAid kit (MBI fermentas).

    Southern Blot:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Plasmid DNA from 2 ml of culture was prepared using miniprep columns (Qiagen), and the DNA was resolved by electrophoresis through a 1% agarose gel containing 5 μg of chloroquine (Sigma)/ml in Tris-acetate-EDTA running buffer.

    Generated:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Plasmid DNA from 2 ml of culture was prepared using miniprep columns (Qiagen), and the DNA was resolved by electrophoresis through a 1% agarose gel containing 5 μg of chloroquine (Sigma)/ml in Tris-acetate-EDTA running buffer.

    DNA Labeling:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Plasmid DNA from 2 ml of culture was prepared using miniprep columns (Qiagen), and the DNA was resolved by electrophoresis through a 1% agarose gel containing 5 μg of chloroquine (Sigma)/ml in Tris-acetate-EDTA running buffer.

    Polymerase Chain Reaction:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The cDNA amplification was carried out according to a standard laboratory PCR protocol using forward primer cx16-15F (5′-GCG CTCGAG GCGTTGCTGACTCTGCAAGG-3′) and reverse primer cx16-15R (5′-GC GGATCC GCACTGCCACTGTAACTGAT-3′) (IDT, Coralville, IA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Sonication:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Recombinant:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. Recombinant plasmid p15-16A (aa 17 to 247) was isolated from ampicillin-resistant clones using a ZR plasmid miniprep kit (Zymo Research, Irvine, CA).

    Mutagenesis:

    Article Title: Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast
    Article Snippet: Escherichia coli strains were transformed with pSG1044 ( divIVA–gfpS65T ) and selected on ampicillin plates (50 µg/ml). .. For the minB mutant P678-54 and cold-sensitive FtsZ strain PB143(pDB346), competent cells were prepared using the TransformAid kit (MBI fermentas). .. Schizosaccharomyces pombe strains were transformed with plasmid DNA by the lithium chloride procedure , essentially as described by .

    Purification:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The purified EqCXCL16 fragment was digested with the XhoI and BamHI restriction enzymes and ligated (Rapid DNA ligation kit; Thermo Scientific, Rockford, IL) into the bacterial expression vector pET15b (EMD Millipore Novagen, Temecula, CA) following digestion with the same restriction enzymes. .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Sequencing:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The sequence encoding the entire EqCXCL16 without the first 16 amino acid residues from the signal sequences was amplified from cDNA made from mRNA extracted from equine monocytes using a Smart cDNA synthesis kit (Clontech Laboratories Inc., Mountain View, CA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    IA:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The cDNA amplification was carried out according to a standard laboratory PCR protocol using forward primer cx16-15F (5′-GCG CTCGAG GCGTTGCTGACTCTGCAAGG-3′) and reverse primer cx16-15R (5′-GC GGATCC GCACTGCCACTGTAACTGAT-3′) (IDT, Coralville, IA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Plasmid Preparation:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The purified EqCXCL16 fragment was digested with the XhoI and BamHI restriction enzymes and ligated (Rapid DNA ligation kit; Thermo Scientific, Rockford, IL) into the bacterial expression vector pET15b (EMD Millipore Novagen, Temecula, CA) following digestion with the same restriction enzymes. .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL). .. Recombinant plasmid p15-16A (aa 17 to 247) was isolated from ampicillin-resistant clones using a ZR plasmid miniprep kit (Zymo Research, Irvine, CA).

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas).

    Agarose Gel Electrophoresis:

    Article Title: Equine Arteritis Virus Uses Equine CXCL16 as an Entry Receptor
    Article Snippet: The amplified EqCXCL16 fragment (amino acids [aa] 17 to 247) was run on a 1% agarose gel (E-Gel EX; Life Technologies, Grand Island, NY) and purified with a commercial kit (Zymoclean gel recovery kit; Zymo Research, Irvine, CA). .. The resultant plasmid construct (p15-16A) was used to transform E. coli NovaBlue (EMD Millipore Novagen, Temecula, CA) using a TransformAid kit (Thermo Scientific, Rockford, IL).

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas).

    Produced:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Plasmid DNA from 2 ml of culture was prepared using miniprep columns (Qiagen), and the DNA was resolved by electrophoresis through a 1% agarose gel containing 5 μg of chloroquine (Sigma)/ml in Tris-acetate-EDTA running buffer.

    Marker:

    Article Title: Alteration of Escherichia coli Topoisomerase IV to Novobiocin Resistance
    Article Snippet: Strains 2817 ( gyrB + parE + acr ), 2818 ( gyrB + parE R132C acr ), 2834 ( gyrB234 parE + acr ), and 2832 ( gyrB234 parE R132C acr ) were transformed with pBR322 (4.4 kb) using the Transformaid kit (Fermentas). .. Plasmid DNA from 2 ml of culture was prepared using miniprep columns (Qiagen), and the DNA was resolved by electrophoresis through a 1% agarose gel containing 5 μg of chloroquine (Sigma)/ml in Tris-acetate-EDTA running buffer.

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  • 96
    Thermo Fisher transformaid bacterial transformation kit
    Transformaid Bacterial Transformation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
    Average 96 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    transformaid bacterial transformation kit - by Bioz Stars, 2019-12
    96/100 stars
      Buy from Supplier

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