transformaid bacterial transformation kit  (Thermo Fisher)


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    Name:
    TransformAid Bacterial Transformation Kit
    Description:
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    Catalog Number:
    k2710
    Price:
    None
    Applications:
    Cloning|Transformation
    Category:
    Competent Cells Strains
    Buy from Supplier


    Structured Review

    Thermo Fisher transformaid bacterial transformation kit
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
    Average 99 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    transformaid bacterial transformation kit - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    DNA Ligation:

    Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
    Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a transformation kit (MBI Fermentas, USA). .. Preliminary identification of recombinant clones was done by blue/white screening.

    Article Title: Allelic Variation in CXCL16 Determines CD3+ T Lymphocyte Susceptibility to Equine Arteritis Virus Infection and Establishment of Long-Term Carrier State in the Stallion
    Article Snippet: .. Ligation and transformation were performed using Rapid DNA Ligation and TransformAid kits (Thermo Scientific), respectively. .. Recombinant plasmids p15-16A (aa 17–247), p15-16B (aa 25–199), and p15-R6 were isolated from ampicillin-resistant clones using ZR Plasmid Miniprep™ Kit (Zymo Research).

    Ligation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Allelic Variation in CXCL16 Determines CD3+ T Lymphocyte Susceptibility to Equine Arteritis Virus Infection and Establishment of Long-Term Carrier State in the Stallion
    Article Snippet: .. Ligation and transformation were performed using Rapid DNA Ligation and TransformAid kits (Thermo Scientific), respectively. .. Recombinant plasmids p15-16A (aa 17–247), p15-16B (aa 25–199), and p15-R6 were isolated from ampicillin-resistant clones using ZR Plasmid Miniprep™ Kit (Zymo Research).

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Mutagenesis:

    Article Title: Promiscuous targeting of Bacillus subtilis cell division protein DivIVA to division sites in Escherichia coli and fission yeast
    Article Snippet: .. For the minB mutant P678-54 and cold-sensitive FtsZ strain PB143(pDB346), competent cells were prepared using the TransformAid kit (MBI fermentas). .. Schizosaccharomyces pombe strains were transformed with plasmid DNA by the lithium chloride procedure , essentially as described by .

    TA Cloning:

    Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
    Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a transformation kit (MBI Fermentas, USA). .. Preliminary identification of recombinant clones was done by blue/white screening.

    Construct:

    Article Title: Cloning and expression of cyclophilin from Platanus orientalis pollens in Escherichia coli
    Article Snippet: .. The resulting construct was transformed into competent TOP10 E. coli cells (Invitrogen, Carlsbad, CA) with Transformation kit (Fermentas). .. The plasmids were sequenced and, based on the obtained sequence, new primers for cloning in pET32b+ plasmid were designed.

    Electroporation Bacterial Transformation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞
    Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Protein Expression and Purification —The Δ65iNOSoxy W188H mutant with a His6 tag at the C terminus was overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector.

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Incubation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Polymerase Chain Reaction:

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
    Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a transformation kit (MBI Fermentas, USA). .. Preliminary identification of recombinant clones was done by blue/white screening.

    Transformation Assay:

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Cloning and expression of cyclophilin from Platanus orientalis pollens in Escherichia coli
    Article Snippet: .. The resulting construct was transformed into competent TOP10 E. coli cells (Invitrogen, Carlsbad, CA) with Transformation kit (Fermentas). .. The plasmids were sequenced and, based on the obtained sequence, new primers for cloning in pET32b+ plasmid were designed.

    Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞
    Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Protein Expression and Purification —The Δ65iNOSoxy W188H mutant with a His6 tag at the C terminus was overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector.

    Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
    Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a transformation kit (MBI Fermentas, USA). .. Preliminary identification of recombinant clones was done by blue/white screening.

    Article Title: Allelic Variation in CXCL16 Determines CD3+ T Lymphocyte Susceptibility to Equine Arteritis Virus Infection and Establishment of Long-Term Carrier State in the Stallion
    Article Snippet: .. Ligation and transformation were performed using Rapid DNA Ligation and TransformAid kits (Thermo Scientific), respectively. .. Recombinant plasmids p15-16A (aa 17–247), p15-16B (aa 25–199), and p15-R6 were isolated from ampicillin-resistant clones using ZR Plasmid Miniprep™ Kit (Zymo Research).

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Recombinant:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Plasmid Preparation:

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
    Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a transformation kit (MBI Fermentas, USA). .. Preliminary identification of recombinant clones was done by blue/white screening.

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  • 99
    Thermo Fisher transformaid kits
    Transformaid Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transformaid kits/product/Thermo Fisher
    Average 99 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    transformaid kits - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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