transformaid bacterial transformation kit (Thermo Fisher)
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TransformAid Bacterial Transformation Kit
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K2711
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Thermo Fisher
transformaid bacterial transformation kit
https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
Average 96 stars, based on 23 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
Average 96 stars, based on 23 article reviews
Price from $9.99 to $1999.99
transformaid bacterial transformation kit - by Bioz Stars,
2020-01
96/100 stars
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Clone Assay:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Ligated DNA was transformed into E . coli ER2738 using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: Paragraph title: Primers design and cloning of Balat_0669 ... E. coli DH5α was transformed with the ligation mixtures using the Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Paragraph title: 2.7. Cloning of the Genomic Region ... DH5α E. coli cell was used for transformation of the plasmid using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Article Title: Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Transformations were performed using a Amplification:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: The primer pair 0669-F (5′-CCATATGACGACGAGCACAC-3′) and 0669-R (5′-AAGCTTCACGCCACCTCATG-3′) was designed according to the gene sequence of the homolog of Balat_0669 in B. animalis subsp. lactis DSM 10140, to obtain the whole amplification of the open reading frame (orf ). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: 5′-rapid amplification of cDNA ends (RACE) library was constructed with the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA) following manufacturer’s instructions. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Whole Genome Amplification:Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: PCR was carried out in a 25 µl reaction volume containing 1 µl of diluted WGA product, 12.5 µl of 2× Xtreme Buffer, 500 µM (each) of dNTPs, 0.02 U of KOD Xtreme™ Hot Start DNA Polymerase, and 0.6 pmol of each primer. .. A Binding Assay:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the Molecular Cloning:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TA Cloning:Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Construct:Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: To quantify the viral loads, standard curves were constructed from a dilution series of cloned plasmids containing inserts in the conserved region of each virus in initial concentrations of 5.1 × 109 IU/mL and 4.9 × 109 IU/mL for HBV and HCV, respectively, with a total of eight points in each curve. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. From the second plasmid on, transformation was performed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: 5′-rapid amplification of cDNA ends (RACE) library was constructed with the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA) following manufacturer’s instructions. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Electrophoresis:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: Ligation mixtures were transformed using a Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Digested fragments were separated by electrophoresis in a 2% agarose gel, visualized under UV light, and compared with digest patterns from monoclonal cultures of Symbiodinium sp., Breviolum sp., Cladocopium sp. and Durusdinium sp., kindly donated by Dr. Mark Warner (University of Delaware, DE, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Expressing:Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: Paragraph title: Gene Expression ... From the second plasmid on, transformation was performed using Modification:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Ligated DNA was transformed into E . coli ER2738 using the Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: From the second plasmid on, transformation was performed using Article Title: Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a Transformation Assay:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: The corresponding oligonucleotides were annealed (cf. ) and separately ligated into Acc65 I / Eag I cleaved M13KE-phage DNA. .. Ligated DNA was transformed into E . coli ER2738 using the Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Countercurrent Chromatography:Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the Sequencing:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: Paragraph title: Cloning and sequencing ... Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: The primer pair 0669-F (5′-CCATATGACGACGAGCACAC-3′) and 0669-R (5′-AAGCTTCACGCCACCTCATG-3′) was designed according to the gene sequence of the homolog of Balat_0669 in B. animalis subsp. lactis DSM 10140, to obtain the whole amplification of the open reading frame (orf ). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: Degenerate primers: forward P450F (5′-GARACIYTIMGNAARTAYCC-3′) and reverse P450R (5′-ATRCADATICKIGGNCCYTC-3′) based on motif of family 6 P450 were used to generate partial sequence of family 6 P450 fragments ( ). .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Paragraph title: DNA extraction, 28S RFLP and ITS2 sequencing ... Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: Paragraph title: 2.6 Immunoglobulin heavy and light chain V(D)J sequencing ... The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Southern Blot:Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Ligation:Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the Generated:Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Both blunt-end PCR products were generated using KOD Xtreme™ Hot Start DNA Polymerase (Toyobo Novagen, EMD Biosciences Inc., San Diego, CA). .. A DNA Sequencing:Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Electroporation Bacterial Transformation:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: The corresponding oligonucleotides were annealed (cf. ) and separately ligated into Acc65 I / Eag I cleaved M13KE-phage DNA. .. Ligated DNA was transformed into E . coli ER2738 using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Article Title: Article Snippet: Restriction digestions, cloning, and bacterial growth were performed using standard procedures. .. Transformations were performed using a Recombinant:Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using DNA Extraction:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: Ligation mixtures were transformed using a Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Paragraph title: DNA extraction, 28S RFLP and ITS2 sequencing ... Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Nucleic Acid Electrophoresis:Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. Mutagenesis:Article Title: Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a Isolation:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: PcP190 satDNA was isolated by PCR using the primers P190F (5′-AGACTGGCTGGGAATCCCAG-3′) and P190R (5′-AGCTGCTGCGATCTGACAAGG-3′), described previously by . .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: PcP190 satDNA was isolated by PCR using the primers P190F (5′-AGACTGGCTGGGAATCCCAG-3′) and P190R (5′-AGCTGCTGCGATCTGACAAGG-3′), described previously by . .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: Allelic bands showing polymorphism between the donor plant and the gamete-derived lines were isolated from the gel, as described by Sambrook and Russell , and sequenced. .. Ligation mixtures were transformed using a Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: To obtain PcP190 satDNA probes, PcP190 sequences were isolated from the genomic DNA of Pseudis fusca . .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: RNA was isolated from whole tissues and genomic DNA destroyed as described above. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Purification:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Polymerase Chain Reaction:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus Article Snippet: Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD). .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Article Title: Article Snippet: Transformations were performed using a Gel Extraction:Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A Chloramphenicol Acetyltransferase Assay:Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: If a single clade were found in a sample, the ribosomal internal transcript spacer 2 region (ITS2 rDNA) was amplified, using the ITSint-for2 ( 5’–GAA TTG CAG AAC TCC GTG–3’ ) [ ] and ITS2-rev ( 5’–GGG ATC CAT ATG CTT AAG TTC AGC GGG T–3’ ) primers [ ], and sequenced. .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using In Situ Hybridization:Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Plasmid Preparation:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2 Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: Ligation mixtures were transformed using a Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 ( Article Title: Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a Software:Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections Article Snippet: The cloning and transformations were performed using a CloneJET PCR Cloning Kit and Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: A Positron Emission Tomography:Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the Agarose Gel Electrophoresis:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean Article Snippet: Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using Chromosome Walking:Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125 Article Snippet: Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151 Article Snippet: A Spectrophotometry:Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the Alkaline Lysis:Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: Ligation mixtures were transformed using a CTG Assay:Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae) Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the Staining:Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines Article Snippet: DNA bands were stained with 0.5 mg/ml ethidium bromide and photographed using a G-box under UV light. .. Ligation mixtures were transformed using a Chick Chorioallantoic Membrane Assay:Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The |