transformaid bacterial transformation kit  (Thermo Fisher)


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    Name:
    TransformAid Bacterial Transformation Kit
    Description:
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    Catalog Number:
    k2710
    Price:
    None
    Applications:
    Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher transformaid bacterial transformation kit
    Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
    Average 97 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    transformaid bacterial transformation kit - by Bioz Stars, 2020-09
    97/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Centrifugation:

    Article Title: A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
    Article Snippet: .. Following this incubation, cells were pelleted by centrifugation at 10,000 × g for 10 seconds and the transformation mixture was removed with a pipettor. .. Cells were resuspended in 1 ml sterile deionized water, pelleted by centrifugation at 10,000 × g for 10 seconds, and the water was removed with a pipettor.

    Amplification:

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    Agarose Gel Electrophoresis:

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    DNA Ligation:

    Article Title: Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
    Article Snippet: .. The restriction enzymes, DNA ligation kit, genomic DNA purification kit, GeneJET plasmid Miniprep kit, bacterial transformation kit, and IPTG were purchased from Fermentas, whereas KOD Hot Start DNA polymerase was purchased from Novagen. .. All other Chemicals were purchased from Merck (Darmstadt, Germany) and are either of analytical or HPLC grade.

    Ligation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Orientation-dependent toxic effect of human papillomavirus type 33 long control region DNA in Escherichia coli cells
    Article Snippet: .. Ligation was performed with T4 DNA Ligase (New England Biolabs, Ipswich, MA, USA) at room temperature for 2 h. The TransformAid Bacterial Transformation Kit (Thermo Fischer Scientific, Waltham, MA, USA) was used to transform ligation products into XL1, XL1-Blue or DH5α E. coli strains according to the protocol of the manufacturer. .. Culturing of E. coli bacteria was performed on Luria Bertani (LB) agar containing 100 µg/ml ampicillin at 37 °C for about 16 h or at 25 °C for about 48 h. In the second approach (TA cloning), the TOPO TA Cloning Kit with One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA, USA) was used to clone the selected full-length HPV33 LCR variants into a cloning vector (PCR 2.1-TOPO vector).

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Purification:

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    Electrophoresis:

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    Electroporation Bacterial Transformation:

    Article Title: Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
    Article Snippet: .. The restriction enzymes, DNA ligation kit, genomic DNA purification kit, GeneJET plasmid Miniprep kit, bacterial transformation kit, and IPTG were purchased from Fermentas, whereas KOD Hot Start DNA polymerase was purchased from Novagen. .. All other Chemicals were purchased from Merck (Darmstadt, Germany) and are either of analytical or HPLC grade.

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞
    Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Protein Expression and Purification —The Δ65iNOSoxy W188H mutant with a His6 tag at the C terminus was overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector.

    Article Title: Orientation-dependent toxic effect of human papillomavirus type 33 long control region DNA in Escherichia coli cells
    Article Snippet: .. Ligation was performed with T4 DNA Ligase (New England Biolabs, Ipswich, MA, USA) at room temperature for 2 h. The TransformAid Bacterial Transformation Kit (Thermo Fischer Scientific, Waltham, MA, USA) was used to transform ligation products into XL1, XL1-Blue or DH5α E. coli strains according to the protocol of the manufacturer. .. Culturing of E. coli bacteria was performed on Luria Bertani (LB) agar containing 100 µg/ml ampicillin at 37 °C for about 16 h or at 25 °C for about 48 h. In the second approach (TA cloning), the TOPO TA Cloning Kit with One Shot TOP10 chemically competent E. coli cells (Invitrogen, Carlsbad, CA, USA) was used to clone the selected full-length HPV33 LCR variants into a cloning vector (PCR 2.1-TOPO vector).

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Incubation:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Article Title: A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
    Article Snippet: .. Following this incubation, cells were pelleted by centrifugation at 10,000 × g for 10 seconds and the transformation mixture was removed with a pipettor. .. Cells were resuspended in 1 ml sterile deionized water, pelleted by centrifugation at 10,000 × g for 10 seconds, and the water was removed with a pipettor.

    DNA Purification:

    Article Title: Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
    Article Snippet: .. The restriction enzymes, DNA ligation kit, genomic DNA purification kit, GeneJET plasmid Miniprep kit, bacterial transformation kit, and IPTG were purchased from Fermentas, whereas KOD Hot Start DNA polymerase was purchased from Novagen. .. All other Chemicals were purchased from Merck (Darmstadt, Germany) and are either of analytical or HPLC grade.

    Polymerase Chain Reaction:

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

    Transformation Assay:

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞
    Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Protein Expression and Purification —The Δ65iNOSoxy W188H mutant with a His6 tag at the C terminus was overexpressed in E. coli strain BL21(DE3) using a modified pCWori vector.

    Article Title: A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
    Article Snippet: .. Following this incubation, cells were pelleted by centrifugation at 10,000 × g for 10 seconds and the transformation mixture was removed with a pipettor. .. Cells were resuspended in 1 ml sterile deionized water, pelleted by centrifugation at 10,000 × g for 10 seconds, and the water was removed with a pipettor.

    Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide
    Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using TransformAid TM Bacterial Transformation Kit (Fermentas, EU). .. After extraction of recombinant plasmids from transformed E. coli , PCR analysis was applied to check the proper insertion of the gene in the recombinant plasmid using T7 promoter-specific primers.

    Recombinant:

    Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity
    Article Snippet: .. The TransformAid Bacterial Transformation Kit (MBI, Fermentas) was used for transforming 10 μl of ligation mixture into competent E. coli DH5α cells, spread plated on LB solid medium containing 100 μg/ml ampicillin and incubated at 37°C for 12 h. The recombinant clones were analysed for the presence and orientation of the DNA insert using restriction digestion analysis. .. The plasmid DNA was isolated from an overnight bacterial culture using a convenient plasmid Miniprep method.

    Plasmid Preparation:

    Article Title: Engineering the Production of Major Catechins by Escherichia coli Carrying Metabolite Genes of Camellia sinensis
    Article Snippet: .. The restriction enzymes, DNA ligation kit, genomic DNA purification kit, GeneJET plasmid Miniprep kit, bacterial transformation kit, and IPTG were purchased from Fermentas, whereas KOD Hot Start DNA polymerase was purchased from Novagen. .. All other Chemicals were purchased from Merck (Darmstadt, Germany) and are either of analytical or HPLC grade.

    Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India
    Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Transform-aid bacterial transformation kit (Fermentas, Germany) as per manufacturer's protocol. .. Positive transformants were selected by colony PCR with the above mentioned primers and restriction enzyme digestion of recombinant plasmid with Eco RI.

    Article Title: Unraveling the Sex Chromosome Heteromorphism of the Paradoxical Frog Pseudis tocantins
    Article Snippet: .. The amplified fragments of the PcP190 and 5S rDNA sequences were analyzed by electrophoresis in 1% agarose gel, purified using the Wizard SV Gel and PCR Clean-up System (Promega), ligated into pGEM-T Easy Vector (Promega) and introduced into an E . coli JM109 strain employing the TransformationAid Bacterial Transformation Kit (Fermentas), following the manufacturer’s instructions. .. Recombinant colonies were identified and plasmid extraction was performed using the mini-prep method described by Sambrook and Russel [ ].

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  • 85
    Thermo Fisher transformaid bacterial transformation kits
    Transformaid Bacterial Transformation Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transformaid bacterial transformation kits/product/Thermo Fisher
    Average 85 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    transformaid bacterial transformation kits - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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