transformaid bacterial transformation kit (Thermo Fisher)
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Name:
TransformAid Bacterial Transformation Kit
Description:
Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
Catalog Number:
k2710
Price:
None
Applications:
Cloning|Transformation
Category:
Competent Cells Strains
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Structured Review
Thermo Fisher
transformaid bacterial transformation kit
Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
Average 99 stars, based on 60 article reviews
Price from $9.99 to $1999.99
Thermo Scientific TransformAid Bacterial Transformation Kit sufficient for 20 transformations uses a novel method for rapid preparation of chemically competent E coli cells from overnight bacterial cultures or bacterial colonies The key component of this system is the unique T solution which produces competent cells in a few easy steps The quick and convenient procedure guarantees transformation efficiencies of more than 107 transformants per µg of plasmid DNA ideal for routine cloning experiments The option to use bacterial colonies for preparation of competent cells adds flexibility to any cloning experiment The TransformAid Bacterial Transformation Kit can be used with most E coli strains commonly used for cloning JM107 JM109 XL1 Blue SURE TOPP2 W3110 NM527 AD494 CJ236 GM2163 C600 BL21 M15 NM522 HB101 ER2267 Highlights• Efficient more than 107 transformants per µg of plasmid DNA• Practical E coli competent cells can be prepared from an overnight culture or from bacterial colonies• Fast less than 1 hour from the overnight bacterial culture to cell plating Starting from bacterial colonies the procedure requires only 2 5 hours• Easy all procedures are performed on ice on your bench Brief centrifugations are carried out in regular microcentrifugesApplications• Routine cloning experiments• Blunt end cloning• TA cloningIncludes• C Medium• T Solution A • T Solution B • Detailed ProtocolNoteCompetent cells prepared with TransformAid Bacterial Transformation Kit are suitable for direct use only Freezing down and storage at 70°C is not recommended Related ProductsTransformAid Bacterial Transformation Kit
https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
Average 99 stars, based on 60 article reviews
Price from $9.99 to $1999.99
transformaid bacterial transformation kit - by Bioz Stars,
2021-01
99/100 stars
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Clone Assay:Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity Article Snippet: .. The DNA Ligation:Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2 Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a Article Title: Allelic Variation in CXCL16 Determines CD3+ T Lymphocyte Susceptibility to Equine Arteritis Virus Infection and Establishment of Long-Term Carrier State in the Stallion Article Snippet: .. 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For the minB mutant P678-54 and cold-sensitive FtsZ strain PB143(pDB346), competent cells were prepared using the TA Cloning:Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2 Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a Construct:Article Title: Cloning and expression of cyclophilin from Platanus orientalis pollens in Escherichia coli Article Snippet: .. The resulting construct was transformed into competent TOP10 E. coli cells (Invitrogen, Carlsbad, CA) with Electroporation Bacterial Transformation:Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity Article Snippet: .. The Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞ Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using Incubation:Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity Article Snippet: .. The Polymerase Chain Reaction:Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2 Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a Transformation Assay:Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Article Title: Cloning and expression of cyclophilin from Platanus orientalis pollens in Escherichia coli Article Snippet: .. The resulting construct was transformed into competent TOP10 E. coli cells (Invitrogen, Carlsbad, CA) with Article Title: Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase *Stabilization and Characterization of a Heme-Oxy Reaction Intermediate in Inducible Nitric-oxide Synthase * S⃞ Article Snippet: .. Mutated plasmids were transformed into BL21(DE3) Escherichia coli cells using the Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2 Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a Article Title: Allelic Variation in CXCL16 Determines CD3+ T Lymphocyte Susceptibility to Equine Arteritis Virus Infection and Establishment of Long-Term Carrier State in the Stallion Article Snippet: .. Ligation and transformation were performed using Rapid DNA Ligation and Article Title: Evaluation of Recombinant Human Growth Hormone Secretion in E. coli using the L-asparaginase II Signal Peptide Article Snippet: .. Competent E. coil BL21 (DE3) cells were transformed with ligation mixture using Recombinant:Article Title: Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity Article Snippet: .. The Plasmid Preparation:Article Title: Isolation and genetic characterization of Japanese encephalitis virus from equines in India Article Snippet: .. The PCR products were ligated into a pGEMT-Easy vector (Promega, USA) and transformed into E. coli JM109 cells (Promega, USA) using Article Title: Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2 Article Snippet: .. The Taq DNA polymerase-generated PCR products from each serotype were separately ligated to pDrive TA cloning vector (Qiagen, USA) and transformed into E. coli JM109 by rapid DNA ligation and a |