transformaid bacterial transformation kit  (Thermo Fisher)


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    Name:
    TransformAid Bacterial Transformation Kit
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    Catalog Number:
    K2711
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    Score:
    85
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    Thermo Fisher transformaid bacterial transformation kit

    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
    Average 96 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    transformaid bacterial transformation kit - by Bioz Stars, 2020-01
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: Paragraph title: Primers design and cloning of Balat_0669 ... E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD). .. Sequences obtained were analyzed to verify the presence of P450 consensus region.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Each transformed sample was plated in two Luria-Bertani (LB) agar plates and kept at 37°C overnight for the growth of bacterial colonies.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Paragraph title: 2.7. Cloning of the Genomic Region ... DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711).

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Article Title:
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Amplification:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations. .. The procedures for the selection of the recombinant clones and the extraction of the plasmidial DNA were those proposed by .

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol.

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: The primer pair 0669-F (5′-CCATATGACGACGAGCACAC-3′) and 0669-R (5′-AAGCTTCACGCCACCTCATG-3′) was designed according to the gene sequence of the homolog of Balat_0669 in B. animalis subsp. lactis DSM 10140, to obtain the whole amplification of the open reading frame (orf ). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711).

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: 5′-rapid amplification of cDNA ends (RACE) library was constructed with the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA) following manufacturer’s instructions. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ).

    Whole Genome Amplification:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: PCR was carried out in a 25 µl reaction volume containing 1 µl of diluted WGA product, 12.5 µl of 2× Xtreme Buffer, 500 µM (each) of dNTPs, 0.02 U of KOD Xtreme™ Hot Start DNA Polymerase, and 0.6 pmol of each primer. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Binding Assay:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Molecular Cloning:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    TA Cloning:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol.

    Construct:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: To quantify the viral loads, standard curves were constructed from a dilution series of cloned plasmids containing inserts in the conserved region of each virus in initial concentrations of 5.1 × 109 IU/mL and 4.9 × 109 IU/mL for HBV and HCV, respectively, with a total of eight points in each curve. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions.

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: 5′-rapid amplification of cDNA ends (RACE) library was constructed with the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA) following manufacturer’s instructions. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ).

    Electrophoresis:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. Plasmid DNA isolation was performed using the alkaline lysis method (Birnboim and Doly, ).

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Digested fragments were separated by electrophoresis in a 2% agarose gel, visualized under UV light, and compared with digest patterns from monoclonal cultures of Symbiodinium sp., Breviolum sp., Cladocopium sp. and Durusdinium sp., kindly donated by Dr. Mark Warner (University of Delaware, DE, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Expressing:

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: Paragraph title: Gene Expression ... From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions.

    Modification:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. Protein expression studies were carried out under ‘autoinduction’ conditions.

    Article Title:
    Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Transformation Assay:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: The corresponding oligonucleotides were annealed (cf. ) and separately ligated into Acc65 I / Eag I cleaved M13KE-phage DNA. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol. .. The bacterial suspensions were plated on solid LB containing ampicillin (100 μg/mL), X-gal (2% in dimethylformamide) and IPTG (50 mM) and were incubated overnight at 37°C.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. A single isolated colony from a selective LB agar medium (supplemented with 200 μg/ml ampicillin) was picked out for overnight bacterial culture.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Each transformed sample was plated in two Luria-Bertani (LB) agar plates and kept at 37°C overnight for the growth of bacterial colonies.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711). .. Subsequently, clones were confirmed by clones' confirmation PCR.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. The main SH production strains generated in this study are listed in .

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Countercurrent Chromatography:

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Sequencing:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Paragraph title: Molecular Cloning of Phage Binding Sequence Motifs and Variants Thereof ... Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Paragraph title: Cloning and sequencing ... Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of the complete genome of HPV-125 ... TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Paragraph title: Amplification, sequencing and cloning of HPV-150 ... A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: The primer pair 0669-F (5′-CCATATGACGACGAGCACAC-3′) and 0669-R (5′-AAGCTTCACGCCACCTCATG-3′) was designed according to the gene sequence of the homolog of Balat_0669 in B. animalis subsp. lactis DSM 10140, to obtain the whole amplification of the open reading frame (orf ). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Nucleotide sequences were obtained from an ABI PRISM 3130 XL Genetic Analyzer (Hitachi, Tokyo, Japan) by the Facility of DNA Sequencing of the Chemistry Institute of the University of São Paulo and were deposited in the GenBank under accession numbers MH571141–MH571149.

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: Degenerate primers: forward P450F (5′-GARACIYTIMGNAARTAYCC-3′) and reverse P450R (5′-ATRCADATICKIGGNCCYTC-3′) based on motif of family 6 P450 were used to generate partial sequence of family 6 P450 fragments ( ). .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD).

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Paragraph title: DNA extraction, 28S RFLP and ITS2 sequencing ... Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: Paragraph title: 2.6 Immunoglobulin heavy and light chain V(D)J sequencing ... The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ).

    Southern Blot:

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Ligation:

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. A single isolated colony from a selective LB agar medium (supplemented with 200 μg/ml ampicillin) was picked out for overnight bacterial culture.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific). .. The genes were excised from the recombinant plasmid pTZ-669 and pTZ-669His with Nde I-Hin dIII and Nde I-Xho I digestion, respectively.

    Generated:

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Both blunt-end PCR products were generated using KOD Xtreme™ Hot Start DNA Polymerase (Toyobo Novagen, EMD Biosciences Inc., San Diego, CA). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    DNA Sequencing:

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. The inserts were sequenced using the BigDye Terminator Kit (Applied Biosystems, Waltham, MA, USA) after being PCR-amplified with T7 and SP6 universal primers.

    Electroporation Bacterial Transformation:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions. .. Recombinant colonies were selected, plasmid DNA was extracted following , and the inserts were amplified by PCR using the T7 and SP6 universal primers.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: The corresponding oligonucleotides were annealed (cf. ) and separately ligated into Acc65 I / Eag I cleaved M13KE-phage DNA. .. Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations. .. The procedures for the selection of the recombinant clones and the extraction of the plasmidial DNA were those proposed by .

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol. .. The bacterial suspensions were plated on solid LB containing ampicillin (100 μg/mL), X-gal (2% in dimethylformamide) and IPTG (50 mM) and were incubated overnight at 37°C.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. A single isolated colony from a selective LB agar medium (supplemented with 200 μg/ml ampicillin) was picked out for overnight bacterial culture.

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with the GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct inserts by size analysis and sequencing with the pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD). .. Sequences obtained were analyzed to verify the presence of P450 consensus region.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Each transformed sample was plated in two Luria-Bertani (LB) agar plates and kept at 37°C overnight for the growth of bacterial colonies.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711). .. Subsequently, clones were confirmed by clones' confirmation PCR.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. The main SH production strains generated in this study are listed in .

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Article Title:
    Article Snippet: Restriction digestions, cloning, and bacterial growth were performed using standard procedures. .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Oligonucleotides used to construct mutants in nNOS were obtained from Integrated DNA Technologies (Coralville, IA).

    Recombinant:

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations. .. The procedures for the selection of the recombinant clones and the extraction of the plasmidial DNA were those proposed by .

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol. .. The bacterial suspensions were plated on solid LB containing ampicillin (100 μg/mL), X-gal (2% in dimethylformamide) and IPTG (50 mM) and were incubated overnight at 37°C.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD).

    DNA Extraction:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Paragraph title: PcP190 Satellite DNA Isolation and Sequencing ... Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Paragraph title: DNA extraction, 28S RFLP and ITS2 sequencing ... Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Nucleic Acid Electrophoresis:

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Mutagenesis:

    Article Title:
    Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Isolation:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: PcP190 satDNA was isolated by PCR using the primers P190F (5′-AGACTGGCTGGGAATCCCAG-3′) and P190R (5′-AGCTGCTGCGATCTGACAAGG-3′), described previously by . .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: PcP190 satDNA was isolated by PCR using the primers P190F (5′-AGACTGGCTGGGAATCCCAG-3′) and P190R (5′-AGCTGCTGCGATCTGACAAGG-3′), described previously by . .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Paragraph title: Extraction, isolation, cloning and sequencing of the DNA ... The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Paragraph title: Isolation, cloning and sequencing of the PcP190 satellite DNA ... The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: Allelic bands showing polymorphism between the donor plant and the gamete-derived lines were isolated from the gel, as described by Sambrook and Russell , and sequenced. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: To obtain PcP190 satDNA probes, PcP190 sequences were isolated from the genomic DNA of Pseudis fusca . .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Each transformed sample was plated in two Luria-Bertani (LB) agar plates and kept at 37°C overnight for the growth of bacterial colonies.

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: RNA was isolated from whole tissues and genomic DNA destroyed as described above. .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ).

    Purification:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: The reaction mixture (50 μl) contained 2 μl of first-strand cDNA, 7 μM of each primer, 0.2 mM dNTP (deoxyribonucleotide) mix, 2.5 UTaq DNA polymerase recombinant (Fermentas, Glen Burnie, MD), 1×Taq buffer with KCl, and 1.75 mM MgCl2 . .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD). .. Sequences obtained were analyzed to verify the presence of P450 consensus region.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Polymerase Chain Reaction:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: For DNA sequencing, a CloneJET PCR cloning kit (Thermo Scientific, cat. no. K1231) was used for the blunting and ligation steps, according to the protocols supplied by the manufacturer. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The fragments were amplified by conventional PCR and cloned using a TOPO TA Cloning Kit (Invitrogen, California, CA, USA) according to manufacturer's instructions. .. The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. The concentration of recombinant plasmids was determined using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and reactions were performed in a 7500 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using the TaqMan detection system (Life Technologies, Carlsbad, CA, USA).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Plasmids containing overlapping PCR products of HPV-150 were prepared using a CloneJET™ PCR Cloning Kit (Fermentas), according to the manufacturer's instructions. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Article Title: Molecular Cloning and Xenobiotic Induction of Seven Novel Cytochrome P450 Monooxygenases inAedes albopictus
    Article Snippet: Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD). .. Amplicons of ∼250 bp were purified and ligated into pTZ57R/T (Fermentas, Glen Burnie, MD) and cloned using TransformAid Bacterial Transformation Kit (Fermentas, Glen Burnie, MD).

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Sequencing was performed on successfully amplified and purified PCR products in the forward direction using an ABI 3500 genetic analyzer with the BigDye Terminator v3.1 kit (Applied Biosystems, Foster City, CA, USA). .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Each transformed sample was plated in two Luria-Bertani (LB) agar plates and kept at 37°C overnight for the growth of bacterial colonies.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711).

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Article Title:
    Article Snippet: Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Gel Extraction:

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: If a single clade were found in a sample, the ribosomal internal transcript spacer 2 region (ITS2 rDNA) was amplified, using the ITSint-for2 ( 5’–GAA TTG CAG AAC TCC GTG–3’ ) [ ] and ITS2-rev ( 5’–GGG ATC CAT ATG CTT AAG TTC AGC GGG T–3’ ) primers [ ], and sequenced. .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    In Situ Hybridization:

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: Paragraph title: 2.1.2. Amplification of PcP190 Satellite DNA for Further Use as a Probe in Southern Blotting and In Situ Hybridization ... The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: The PCR program used was: (1) 94°C for 8 min; (2) 39 cycles of 94°C for 30 s, 58°C for 1 min and 72°C for 2.5 min; (3) 72°C for 8 min. PCR amplicons were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) and inserted in a plasmid pGEM-T Easy Vector (Promega). .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Identification and Tumour-Binding Properties of a Peptide with High Affinity to the Disialoganglioside GD2
    Article Snippet: Ligated DNA was transformed into E . coli ER2738 using the TransformAid bacterial transformation kit (Fermentas, St. Leon-Rot, Germany) according to the manufacturer’s instructions. .. Positive clones were identified using LB/S-Gal/IPTG-plates.

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: The resulting sequences were purified and ligated to the pGEM-T Easy vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: Subsequently, the DNA fragments were inserted into the pGEM-T Easy Vector (Promega, Madison, Wisconsin, USA). .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Plasmid clones containing overlapping PCR products were prepared using a CloneJET™ PCR Cloning Kit (Fermentas) according to the manufacturer's instructions. .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: The PCR products (789 bp and 796) were cloned into the vector pTZ57R using the InsTAclone PCR cloning kit (Thermo Fisher Scientific, Waltham, MA). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Article Title: Satellite DNA Mapping in Pseudis fusca (Hylidae, Pseudinae) Provides New Insights into Sex Chromosome Evolution in Paradoxical Frogs
    Article Snippet: The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega, Madison, WI, USA). .. The obtained fragments were inserted into a plasmid using the pGEM-T Easy Vector Kit (Promega, USA) and cloned into Escherichia coli JM109 employing the TransformAid Bacterial Transformation Kit (Fermentas, Waltham, MA, USA), according to the manufacturer’s instructions. .. Recombinant colonies were selected, and the plasmids were extracted according to Sambrook et al. [ ].

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

    Article Title: Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
    Article Snippet: Cloning of the PCR amplicon(s) for the genomic region as described above has been performed using pDrive cloning vector (Qiagen PCR Cloning Kit, Catalog number 231124). .. DH5α E. coli cell was used for transformation of the plasmid using Transform Aid Bacterial Transformation Kit (Fermentas, Catalog number K2711). .. Subsequently, clones were confirmed by clones' confirmation PCR.

    Article Title: An Innovative Cloning Platform Enables Large-Scale Production and Maturation of an Oxygen-Tolerant [NiFe]-Hydrogenase from Cupriavidus necator in Escherichia coli
    Article Snippet: The first construct was inserted using heat shock transformation. .. From the second plasmid on, transformation was performed using TransformAid Bacterial Transformation Kit (Fermentas) according to the manufacturer’s instructions. .. The main SH production strains generated in this study are listed in .

    Article Title: Hematopoiesis In The Equine Fetal Liver Suggests Immune Preparedness
    Article Snippet: The lambda chain was chosen as the horse uses primarily this light chain in circulating antibodies ( ). .. The PCR products were purified, ligated into the pJET1.2 vector (CloneJET™ PCR Cloning Kit, Thermo Fisher Scientific Inc.), transformed into either JM107 (TransformAID™ Bacterial Transformation Kit, Thermo Fisher Scientific Inc.) or NEB-5 alpha (New England BioLabs, Ipswich, MA) competent E.coli , expanded, and sequenced at the Cornell University Institute of Biotechnology (Ithaca, NY), as previously described ( ; ). .. Heavy and lambda light chain Ig sequences were obtained from fetal liver, fetal bone marrow, and adult bone marrow (n = 3).

    Article Title:
    Article Snippet: Wild-type and mutant nNOS proteins containing a His6 tag attached to their N termini were overexpressed in Escherichia coli strain BL21(DE3) using a modified pCWori vector as described ( , ). .. Transformations were performed using a TransformAid bacterial transformation kit (Fermentas, Hanover, MD).

    Software:

    Article Title: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
    Article Snippet: The cloning and transformations were performed using a CloneJET PCR Cloning Kit and TransformAid Bacterial Transformation Kit, respectively (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's protocol. .. Assays consisted of 12.5 μL of TaqMan Universal Master Buffer, predetermined concentrations of primers and probes, and 50–100 ng of DNA or cDNA, for a final volume of 25 μL per reaction.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Positron Emission Tomography:

    Article Title: Role of bifidobacteria in the hydrolysis of chlorogenic acid
    Article Snippet: In order to be easily purified from Escherichia coli , the recombinant protein Balat_0669 was also cloned and expressed fused to the C-terminal 6 × His tag carried in the expression vector pET-21b(+). .. E. coli DH5α was transformed with the ligation mixtures using the TransformAid Bacterial Transformation Kit (Thermo Fisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: Before cloning, PCR products were run on gel electrophoresis, excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: Before cloning, PCR products were excised and purified from 1% agarose gel using a GeneJET™ Gel Extraction Kit (Fermentas, Vilnius, Lithuania). .. A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions.

    Article Title: Biogeography of the endosymbiotic dinoflagellates (Symbiodiniaceae) community associated with the brooding coral Favia gravida in the Atlantic Ocean
    Article Snippet: Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol. .. Samples with more than one clade on RFLP screening or with additional unidentified bands were cloned using the CloneJET PCR Cloning Kit (Thermo Scientific, Waltham, MA, USA) with competent E . coli DH5α transformed using TransformAid Bacterial Transformation Kit (Thermo Scientific) following the manufacturer’s protocol.

    Chromosome Walking:

    Article Title: Characterization of a Novel Cutaneous Human Papillomavirus Genotype HPV-125
    Article Snippet: TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the plasmids, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with the GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct inserts by size analysis and sequencing with the pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Article Title: Characterization of Novel Cutaneous Human Papillomavirus Genotypes HPV-150 and HPV-151
    Article Snippet: A TransformAid™ Bacterial Transformation Kit (Fermentas) was used to transform E.coli strain JM107 with the HPV-150 clones, according to the manufacturer's instructions. .. Plasmid clones isolated from the transformed overnight bacterial culture with a GeneJET™ Plasmid Miniprep Kit (Fermentas) were checked for correct HPV inserts by size analysis and sequencing with pJET1.2 sequencing primers, according to the manufacturer's instructions.

    Spectrophotometry:

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Article Title: Sex Chromosome Differentiation in the Frog Genus Pseudis Involves Satellite DNA and Chromosome Rearrangements
    Article Snippet: Integrity of genomic DNA was evaluated by electrophoresis in a 0.8% agarose gel and total genomic DNA was quantified in a Nanodrop (Thermo Fisher, United States) spectrophotometer. .. Plasmids were cloned into E. coli JM-109 bacteria using the TransformAid Bacterial Transformation Kit (Fermentas), according to manufacturer instructions.

    Alkaline Lysis:

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific). .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    CTG Assay:

    Article Title: Chromosomal analysis of Physalaemuskroyeri and Physalaemuscicada ( Anura, Leptodactylidae)
    Article Snippet: Samples of the genomic DNA of Physalaemus cicada and Physalaemus kroyeri were submitted to a PCR using the primers P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) ( ) for the isolation of the PcP190 satellite DNA. .. The recombinant vectors were used to transform Escherichia coli bacteria of the JM109 lineage using a TransformAid™ Bacterial Transformation kit (Fermentas, Burlington, Ontario, Canada), following the maker’s recommendations.

    Article Title: Long-time evolution and highly dynamic satellite DNA in leptodactylid and hylodid frogs
    Article Snippet: To isolate the satellite DNA of each species, DNA samples were subjected to PCR using specific primers: P190F (AGA CTG GCT GGG AAT CCC AG) and P190R (AGC TGC TGC GAT CTG ACA AGG) [ ]. .. The recombinant vectors were transformed into JM109 E. coli with the TransformAid™ Bacterial Transformation Kit (Fermentas, Burlington, Ontario, Canada) according to the manufacturer’s protocol.

    Staining:

    Article Title: Homozygote Depression in Gamete-Derived Dragon-Fruit (Hylocereus) Lines
    Article Snippet: DNA bands were stained with 0.5 mg/ml ethidium bromide and photographed using a G-box under UV light. .. Ligation mixtures were transformed using a TransformAid bacterial transformation kit (Thermo Scientific).

    Chick Chorioallantoic Membrane Assay:

    Article Title: Characterization of Calmodulin-Free Murine Inducible Nitric-Oxide Synthase
    Article Snippet: Resins used for purification of the iNOS proteins were procured from GE Healthcare, USA while all other reagents and chemicals used were obtained either from Sigma-Aldrich, USA or Merck, Germany and were of analytical grade. .. The TransformAid Bacterial Transformation Kit (Fermentas, USA) was used to transform Escherichia coli (E .coli ) strains BL21(DE3) and DH5α with iNOS and CaM plasmid DNAs either separately or together using the expression vector pCW through standard procedures [ ]. .. The transformed bacteria were then grown overnight in cultures and stored at -80°C as glycerol stocks for further use.

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    Thermo Fisher transformaid bacterial transformation kit
    Transformaid Bacterial Transformation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transformaid bacterial transformation kit/product/Thermo Fisher
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    transformaid bacterial transformation kit - by Bioz Stars, 2020-01
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