Structured Review

PerkinElmer trace α32 p atp
Trace α32 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trace α32 p atp/product/PerkinElmer
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
trace α32 p atp - by Bioz Stars, 2020-04
85/100 stars

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Selection:

Article Title: Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
Article Snippet: .. In vitro transcription Pools were transcribed with T7 RNA polymerase, in transcription buffer containing 20 mM each NTP, 25 mM Mg2+ , 40 mM Tris (pH 7.8), 2.5 mM spermidine, 10 mM DTT, 0.01% Triton X-100, 50 U T7 RNA Polymerase, 10 U/μl thermostable inorganic pyrophosphatase (TIPP, New England Biolabs, UK), 200 U/μl RNAsin (Promega) and, for the selection, trace α32 P-ATP (Perkin Elmer). .. The RNA was purified on a PAGE gel, and ethanol (EtOH) precipitated with potassium chloride (KCl).

In Vitro:

Article Title: Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
Article Snippet: .. In vitro transcription Pools were transcribed with T7 RNA polymerase, in transcription buffer containing 20 mM each NTP, 25 mM Mg2+ , 40 mM Tris (pH 7.8), 2.5 mM spermidine, 10 mM DTT, 0.01% Triton X-100, 50 U T7 RNA Polymerase, 10 U/μl thermostable inorganic pyrophosphatase (TIPP, New England Biolabs, UK), 200 U/μl RNAsin (Promega) and, for the selection, trace α32 P-ATP (Perkin Elmer). .. The RNA was purified on a PAGE gel, and ethanol (EtOH) precipitated with potassium chloride (KCl).

Polyacrylamide Gel Electrophoresis:

Article Title: Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
Article Snippet: In vitro transcription Pools were transcribed with T7 RNA polymerase, in transcription buffer containing 20 mM each NTP, 25 mM Mg2+ , 40 mM Tris (pH 7.8), 2.5 mM spermidine, 10 mM DTT, 0.01% Triton X-100, 50 U T7 RNA Polymerase, 10 U/μl thermostable inorganic pyrophosphatase (TIPP, New England Biolabs, UK), 200 U/μl RNAsin (Promega) and, for the selection, trace α32 P-ATP (Perkin Elmer). .. The RNA was purified on a PAGE gel, and ethanol (EtOH) precipitated with potassium chloride (KCl).

Purification:

Article Title: Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
Article Snippet: In vitro transcription Pools were transcribed with T7 RNA polymerase, in transcription buffer containing 20 mM each NTP, 25 mM Mg2+ , 40 mM Tris (pH 7.8), 2.5 mM spermidine, 10 mM DTT, 0.01% Triton X-100, 50 U T7 RNA Polymerase, 10 U/μl thermostable inorganic pyrophosphatase (TIPP, New England Biolabs, UK), 200 U/μl RNAsin (Promega) and, for the selection, trace α32 P-ATP (Perkin Elmer). .. The RNA was purified on a PAGE gel, and ethanol (EtOH) precipitated with potassium chloride (KCl).

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    PerkinElmer trace α32 p atp
    Trace α32 P Atp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trace α32 p atp/product/PerkinElmer
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trace α32 p atp - by Bioz Stars, 2020-04
    85/100 stars
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