total rna isolation solution  (Thermo Fisher)


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    Name:
    Total RNA Lysis Solution
    Description:
    Total RNA Lysis Solution is a 2X concentrate ideal for the chemical disruption of cells and tissue cultured cells primary cell isolates and animal plant tissues prior to RNA or DNA extraction Following downstream purification e g using the 6100 PrepStation intact RNA can be isolated that is free of contaminating protein and metal ions Total RNA Lysis solution is the recommended reagent for the Total RNA Isolation Chemistry System for the ABI PRISM 6100 Nucleic Acid PrepStation For research use only Not for use in diagnostic procedures
    Catalog Number:
    4305895
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|General RNA Purification Reagents & Accessories|RNA Extraction
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher total rna isolation solution
    Total RNA Lysis Solution is a 2X concentrate ideal for the chemical disruption of cells and tissue cultured cells primary cell isolates and animal plant tissues prior to RNA or DNA extraction Following downstream purification e g using the 6100 PrepStation intact RNA can be isolated that is free of contaminating protein and metal ions Total RNA Lysis solution is the recommended reagent for the Total RNA Isolation Chemistry System for the ABI PRISM 6100 Nucleic Acid PrepStation For research use only Not for use in diagnostic procedures
    https://www.bioz.com/result/total rna isolation solution/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total rna isolation solution - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Length variants of the 5′ untranslated region of p53 mRNA and their impact on the efficiency of translation initiation of p53 and its N-truncated isoform ΔNp53
    Article Snippet: The RNA solution was mixed with 7.3 μl 180 mM NMIA (Invitrogen) in DMSO (8–16 mM final concentration). .. After centrifugation, RNA was resuspended in 10 mM Tris pH 8.0 and 0.1 mM EDTA.

    Article Title: The Munich MIDY Pig Biobank – A unique resource for studying organ crosstalk in diabetes
    Article Snippet: RNA was precipitated by addition of 0.5 volumes of isopropanol to the aqueous phase and sedimented by centrifugation. .. Each RNA solution was quantified by UV/VIS spectrometry (Nanodrop ND1000), and the 260/280 ratio was determined.

    Amplification:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted. .. Previous protocols additionally involved washing the beads three times after the ligation step, and then eluting material off the beads prior to amplification.

    Article Title: Transcriptional Mutagenesis Induced by 8-Oxoguanine in Mammalian Cells
    Article Snippet: Contaminating DNA was eliminated from the RNA solution by two treatments with the DNA-free kit (Ambion). .. We then used about 50 ng of RNA for RT-PCR with Superscript II (Invitrogen) as a reverse transcriptase and Taq DNA polymerase for amplification (New England Biolabs), using LBRT1 and LBRT2 as primers .

    Construct:

    Article Title: Transcriptional Mutagenesis Induced by 8-Oxoguanine in Mammalian Cells
    Article Snippet: RNA extraction and RT–PCR RNA was extracted from MRC5V1 cells 24 hours after transfection with the (8OG/Stop)445 construct. .. Contaminating DNA was eliminated from the RNA solution by two treatments with the DNA-free kit (Ambion).

    Real-time Polymerase Chain Reaction:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Paragraph title: 2.7. Quantitative Real-Time PCR ... Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions.

    Article Title: Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells in Phenotypic Screening: A Transforming Growth Factor-β Type 1 Receptor Kinase Inhibitor Induces Efficient Cardiac Differentiation
    Article Snippet: Paragraph title: Real-Time Polymerase Chain Reaction Assays ... Cell samples were collected using lysis solution (nucleic acid purification; catalog no. 4305895; Thermo Fisher Scientific).

    Article Title: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats
    Article Snippet: miRNA analysis We used 2.29 μL of RNA solution from 1 mL urine and 1.46 μL of reverse transcriptase solution of the TaqMan MicroRNA RT Kit (ThermoFisher Scientific) to produce complementary DNA (cDNA). .. The obtained cDNA solution (4.5 μL) was used for quantitative PCR (qPCR), qPCR analysis was performed using each miRNA-specific TaqMan primer (0.5 μL) and TaqMan Universal PCR Master Mix (4.5 μL, ThermoFisher Scientific) with an MX3000P system (Agilent; Santa Clara, CA, USA) and CFX Connect (Bio-Rad; Hercules, CA, USA).

    Incubation:

    Article Title: Length variants of the 5′ untranslated region of p53 mRNA and their impact on the efficiency of translation initiation of p53 and its N-truncated isoform ΔNp53
    Article Snippet: The RNA solution was mixed with 7.3 μl 180 mM NMIA (Invitrogen) in DMSO (8–16 mM final concentration). .. Both reactions were incubated for 50 min at 37 °C and RNA was precipitated with 0.3 M sodium acetate, pH 5.2, 1 μl glycogen (20 mg/ml), and 3 vol of ethanol.

    Expressing:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions. .. Reactions were prepared in triplicate for each gene using Taqman gene expression assays.

    Transfection:

    Article Title: Transcriptional Mutagenesis Induced by 8-Oxoguanine in Mammalian Cells
    Article Snippet: RNA extraction and RT–PCR RNA was extracted from MRC5V1 cells 24 hours after transfection with the (8OG/Stop)445 construct. .. Contaminating DNA was eliminated from the RNA solution by two treatments with the DNA-free kit (Ambion).

    Sequencing:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions. .. Taqman PCR probes and gene-specific primer pairs were generated for COX-2, PGDS, and GAPDH using Integrated DNA technologies. qRT-PCR was performed on an Applied Biosystems Prism 7900 Sequence Detection System.

    Ligation:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: PBCV-1 is partially inhibited by dC/G and dG/C base pairs at the 5′-terminus of the donor strand, so we examined whether this might explain the low efficiency of ligation for the GAPDH_2 probe set. .. Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted.

    Buffer Exchange:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted. .. In published RASL-seq protocols, mRNA/probe immobilized beads were washed six times with wash buffer (in addition to a buffer exchange) in order to remove contaminating gDNA and lysis solution.

    Cell Culture:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: Fifty microliters of B cells was finally added to each well, leaving a perimeter of wells containing media only, and then cultured for 7 days in a superhumid incubator to minimize evaporation. .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895).

    Article Title: Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
    Article Snippet: Extraction of RNA Total RNA from 1 × 106 cells was prepared using the QIAamp RNA Blood Mini Kit and RNase-free DNase set (Qiagen, Hilden, Germany) according to the manufacturer's recommendations for cultured cells. .. RNA solution was treated with DNA-free (Ambion, Huntingdon, United Kingdom).

    Generated:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions. .. First-strand cDNA was generated from total RNA for each sample using the cDNA kit (Applied Biosystems 18080200) according to the manufacturer's instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Transcriptional Mutagenesis Induced by 8-Oxoguanine in Mammalian Cells
    Article Snippet: Paragraph title: RNA extraction and RT–PCR ... Contaminating DNA was eliminated from the RNA solution by two treatments with the DNA-free kit (Ambion).

    Nucleic Acid Purification:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895). ..

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: .. Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted. .. In published RASL-seq protocols, mRNA/probe immobilized beads were washed six times with wash buffer (in addition to a buffer exchange) in order to remove contaminating gDNA and lysis solution.

    Article Title: Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells in Phenotypic Screening: A Transforming Growth Factor-β Type 1 Receptor Kinase Inhibitor Induces Efficient Cardiac Differentiation
    Article Snippet: .. Cell samples were collected using lysis solution (nucleic acid purification; catalog no. 4305895; Thermo Fisher Scientific). .. Total RNA was extracted using a semiautomatic prepstation (6100 Nucleic Acid PrepStation; Thermo Fisher Scientific).

    Isolation:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: B cells were isolated by positive selection on CD19+ using EasySep beads and magnet (STEMCELL Technologies) according to the manufacturer's instructions. .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895).

    Article Title: Fatty acids regulate perilipin5 in muscle by activating PPAR? [S]
    Article Snippet: .. Cells were lysed in 500 μl 1× Total RNA Lysis Solution (#4305895, Life Technologies Corporation) per well (12-well plate) and frozen at −80°C before isolation. .. Total RNA from cell extracts was isolated using ABI 6100 Nucleic Acid Prep-Station using the preprogrammed “RNA-Cell method” (Life Technologies Corporation).

    Article Title: Effects of A 4-Week Aerobic Exercise on Lipid Profile and Expression of LXRα in Rat Liver
    Article Snippet: Total RNA was isolated by the RNA-Plus kit (CinnaGen, Iran) according to the manufacturer’s instructions. .. Next, the extracted RNA solution was decontaminated from any DNA using RNase free DNaseI (Fermentas, Lithuania).

    Purification:

    Article Title: Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells in Phenotypic Screening: A Transforming Growth Factor-β Type 1 Receptor Kinase Inhibitor Induces Efficient Cardiac Differentiation
    Article Snippet: Cell samples were collected using lysis solution (nucleic acid purification; catalog no. 4305895; Thermo Fisher Scientific). .. The samples were added to a RNA purification tray (catalog no. 4305673; Thermo Fisher Scientific) and processed using the RNA Cell Method with DNase Wash protocol, as specified by the distributer.

    Polymerase Chain Reaction:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions. .. Taqman PCR probes and gene-specific primer pairs were generated for COX-2, PGDS, and GAPDH using Integrated DNA technologies. qRT-PCR was performed on an Applied Biosystems Prism 7900 Sequence Detection System.

    Article Title: Effects of A 4-Week Aerobic Exercise on Lipid Profile and Expression of LXRα in Rat Liver
    Article Snippet: Next, the extracted RNA solution was decontaminated from any DNA using RNase free DNaseI (Fermentas, Lithuania). .. Realtime (SYBRGreen) polymerase chain reaction (PCR) was carried out in a thermal cycler (BioRad, CA, USA) as suggested by the protocol (TaKaRa, Japan).

    Article Title: Involvement of the Multidomain Regulatory Protein XynR in Positive Control of Xylanase Gene Expression in the Ruminal Anaerobe Prevotella bryantii B14
    Article Snippet: For the nuclease protection assay a 609-bp DIG-labeled probe was prepared by PCR by using a DIG probe synthesis kit (Roche Diagnostics GmbH) and primers Reg-UP and Reg-DOWN (Table ). .. A total-RNA solution was mixed with 600 pg of DIG-labeled probe and 2 μg of yeast RNA (Ambion Inc.), and this was followed by ethanol precipitation.

    Article Title: Urinary Exosome-Derived microRNAs Reflecting the Changes in Renal Function in Cats
    Article Snippet: miRNA analysis We used 2.29 μL of RNA solution from 1 mL urine and 1.46 μL of reverse transcriptase solution of the TaqMan MicroRNA RT Kit (ThermoFisher Scientific) to produce complementary DNA (cDNA). .. The obtained cDNA solution (4.5 μL) was used for quantitative PCR (qPCR), qPCR analysis was performed using each miRNA-specific TaqMan primer (0.5 μL) and TaqMan Universal PCR Master Mix (4.5 μL, ThermoFisher Scientific) with an MX3000P system (Agilent; Santa Clara, CA, USA) and CFX Connect (Bio-Rad; Hercules, CA, USA).

    Quantitative RT-PCR:

    Article Title: Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells
    Article Snippet: Total RNA was extracted using RNA kit (Applied Biosystems 4305895) according to the manufacturer's instructions. .. Taqman PCR probes and gene-specific primer pairs were generated for COX-2, PGDS, and GAPDH using Integrated DNA technologies. qRT-PCR was performed on an Applied Biosystems Prism 7900 Sequence Detection System.

    SYBR Green Assay:

    Article Title: Effects of A 4-Week Aerobic Exercise on Lipid Profile and Expression of LXRα in Rat Liver
    Article Snippet: Next, the extracted RNA solution was decontaminated from any DNA using RNase free DNaseI (Fermentas, Lithuania). .. The PCR mixture contained 10 µL Rotor-Gene SYBR Green PCR Master Mix (TaKaRa, Japan), 3 pM of each primer and 25 ng cDNA in a final volume of 20 µL.

    RNA Extraction:

    Article Title: Transcriptional Mutagenesis Induced by 8-Oxoguanine in Mammalian Cells
    Article Snippet: Paragraph title: RNA extraction and RT–PCR ... Contaminating DNA was eliminated from the RNA solution by two treatments with the DNA-free kit (Ambion).

    Article Title: The Munich MIDY Pig Biobank – A unique resource for studying organ crosstalk in diabetes
    Article Snippet: Paragraph title: RNA extraction and RNA quality indices ... Each RNA solution was quantified by UV/VIS spectrometry (Nanodrop ND1000), and the 260/280 ratio was determined.

    Selection:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: B cells were isolated by positive selection on CD19+ using EasySep beads and magnet (STEMCELL Technologies) according to the manufacturer's instructions. .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895).

    Ethanol Precipitation:

    Article Title: Involvement of the Multidomain Regulatory Protein XynR in Positive Control of Xylanase Gene Expression in the Ruminal Anaerobe Prevotella bryantii B14
    Article Snippet: .. A total-RNA solution was mixed with 600 pg of DIG-labeled probe and 2 μg of yeast RNA (Ambion Inc.), and this was followed by ethanol precipitation. .. The nuclease protection assay was performed by using a Multi-NPA kit (Ambion Inc.) according to the supplier's instructions.

    Evaporation:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: Fifty microliters of B cells was finally added to each well, leaving a perimeter of wells containing media only, and then cultured for 7 days in a superhumid incubator to minimize evaporation. .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895).

    Concentration Assay:

    Article Title: Length variants of the 5′ untranslated region of p53 mRNA and their impact on the efficiency of translation initiation of p53 and its N-truncated isoform ΔNp53
    Article Snippet: .. The RNA solution was mixed with 7.3 μl 180 mM NMIA (Invitrogen) in DMSO (8–16 mM final concentration). ..

    Article Title: The Munich MIDY Pig Biobank – A unique resource for studying organ crosstalk in diabetes
    Article Snippet: RNA was air dried for 5 min at room temperature and solved in DNase/RNase free water to a final concentration of 100–200 ng/μl. .. Each RNA solution was quantified by UV/VIS spectrometry (Nanodrop ND1000), and the 260/280 ratio was determined.

    High Throughput Screening Assay:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: The cost basis of each RNA analysis is particularly important for applications involving large numbers of samples, such as high throughput screening. .. Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted.

    Lysis:

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: .. This PBS wash was repeated one more time and to the remaining 10 μl was added 10 μl of 2× Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895). ..

    Article Title: Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay
    Article Snippet: .. Of those tested, an inexpensive Total RNA Lysis Solution, Nucleic Acid Purification (NAP; Life Technologies, Catalog no. 4305895) buffer was adopted. .. In published RASL-seq protocols, mRNA/probe immobilized beads were washed six times with wash buffer (in addition to a buffer exchange) in order to remove contaminating gDNA and lysis solution.

    Article Title: Fatty acids regulate perilipin5 in muscle by activating PPAR? [S]
    Article Snippet: .. Cells were lysed in 500 μl 1× Total RNA Lysis Solution (#4305895, Life Technologies Corporation) per well (12-well plate) and frozen at −80°C before isolation. .. Total RNA from cell extracts was isolated using ABI 6100 Nucleic Acid Prep-Station using the preprogrammed “RNA-Cell method” (Life Technologies Corporation).

    Article Title: Human Induced Pluripotent Stem Cell-Derived Cardiac Progenitor Cells in Phenotypic Screening: A Transforming Growth Factor-β Type 1 Receptor Kinase Inhibitor Induces Efficient Cardiac Differentiation
    Article Snippet: .. Cell samples were collected using lysis solution (nucleic acid purification; catalog no. 4305895; Thermo Fisher Scientific). .. Total RNA was extracted using a semiautomatic prepstation (6100 Nucleic Acid PrepStation; Thermo Fisher Scientific).

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    Thermo Fisher solution
    Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solution/product/Thermo Fisher
    Average 99 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    solution - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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