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PEQLAB total ribonucleic acid rna
Total Ribonucleic Acid Rna, supplied by PEQLAB, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total ribonucleic acid rna/product/PEQLAB
Average 94 stars, based on 5 article reviews
Price from $9.99 to $1999.99
total ribonucleic acid rna - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. Semi-quantitative real-time polymerase chain reaction (PCR) was performed to quantify the mRNA levels of CCL5 in relation to the housekeeping gene β-actin. cDNA samples were amplified with the Power Sybr Green PCR Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. Semiquantitative real-time polymerase chain reaction (PCR) was performed to quantify the messenger RNA (mRNA) levels of IL-6, IL-11, and LIF in relation to the housekeeping gene β-actin.

Amplification:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. Semi-quantitative real-time polymerase chain reaction (PCR) was performed to quantify the mRNA levels of CCL5 in relation to the housekeeping gene β-actin. cDNA samples were amplified with the Power Sybr Green PCR Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. Complementary DNA (cDNA) samples were amplified using the Power SYBR® Green PCR-Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Agarose Gel Electrophoresis:

Article Title: Clozapine promotes glycolysis and myelin lipid synthesis in cultured oligodendrocytes
Article Snippet: Total ribonucleic acid (RNA) was isolated from OLN-93 cell cultures using guanidinium isothiocyanate/phenol/chloroform (peqGOLD TriFast™, peqlab, Erlangen, Germany). .. One-tenth of each reaction product was electrophoresed on a 1% agarose gel.

In Vitro:

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Human ESCs were decidualized in vitro using 1 μM progesterone and 30 nM 17β-estradiol (EP) plus/minus 5 μg/mL unfractionated heparin or tinzaparin. .. Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA).

Synthesized:

Article Title: High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii
Article Snippet: Briefly, total ribonucleic acid (RNA) was extracted from the m . triceps brachii using the PeqGOLD HP Total RNA kit (Peqlab, Germany), according to the manufacturer’s recommendations. .. Complementary DNA (cDNA) was synthesized from total RNA using the iScript advanced cDNA Synthesis Kit (Bio-Rad Laboratories, California, USA).

Isolation:

Article Title: High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii
Article Snippet: Briefly, total ribonucleic acid (RNA) was extracted from the m . triceps brachii using the PeqGOLD HP Total RNA kit (Peqlab, Germany), according to the manufacturer’s recommendations. .. Isolated RNA was then treated with Turbo DNase (Ambion, Life Technologies, Carlsbad, CA, USA).

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: .. Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. Semi-quantitative real-time polymerase chain reaction (PCR) was performed to quantify the mRNA levels of CCL5 in relation to the housekeeping gene β-actin. cDNA samples were amplified with the Power Sybr Green PCR Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: .. Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. Semiquantitative real-time polymerase chain reaction (PCR) was performed to quantify the messenger RNA (mRNA) levels of IL-6, IL-11, and LIF in relation to the housekeeping gene β-actin.

Article Title: Clozapine promotes glycolysis and myelin lipid synthesis in cultured oligodendrocytes
Article Snippet: .. Total ribonucleic acid (RNA) was isolated from OLN-93 cell cultures using guanidinium isothiocyanate/phenol/chloroform (peqGOLD TriFast™, peqlab, Erlangen, Germany). .. For removing deoxyribonucleic acid (DNA) contamination, 5 μg of the total cell RNA was treated with Turbo DNA-free (Ambion, Austin, TX, USA) according to the manufacturer's instructions.

Sequencing:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. The primers (Invitrogen) were designed using Primer Express primer design software, version 2.0 (Applied Biosystems), with the resulting amplicons having an intron-overlapping sequence.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. The primers (Invitrogen) were designed using Primer Express® Primer Design Software v2.0 (Applied Biosystems) with the resulting amplicons having an intron-overlapping sequence.

Quantitative RT-PCR:

Article Title: High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii
Article Snippet: Paragraph title: Quantitative real time (qRT)-PCR ... Briefly, total ribonucleic acid (RNA) was extracted from the m . triceps brachii using the PeqGOLD HP Total RNA kit (Peqlab, Germany), according to the manufacturer’s recommendations.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: At day 9, cells were stimulated with 50 ng/mL IL-1β for 6 h. Thereafter, the levels of IL-6, IL-11, and LIF were determined by semiquantitative real-time RT-PCR. .. Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Paragraph title: Real-time reverse transcription polymerase chain reaction ... Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Clozapine promotes glycolysis and myelin lipid synthesis in cultured oligodendrocytes
Article Snippet: Membrane transporters for lactate and glucose (Figure 1③–④) The expression of MCT1-4 and GLUT1-4 was tested in OLN-93 cells by reverse transcriptase polymerase chain reaction (RT-PCR). .. Total ribonucleic acid (RNA) was isolated from OLN-93 cell cultures using guanidinium isothiocyanate/phenol/chloroform (peqGOLD TriFast™, peqlab, Erlangen, Germany).

SYBR Green Assay:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. Semi-quantitative real-time polymerase chain reaction (PCR) was performed to quantify the mRNA levels of CCL5 in relation to the housekeeping gene β-actin. cDNA samples were amplified with the Power Sybr Green PCR Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. Complementary DNA (cDNA) samples were amplified using the Power SYBR® Green PCR-Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Concentration Assay:

Article Title: High-intensity high-volume swimming induces more robust signaling through PGC-1α and AMPK activation than sprint interval swimming in m. triceps brachii
Article Snippet: Briefly, total ribonucleic acid (RNA) was extracted from the m . triceps brachii using the PeqGOLD HP Total RNA kit (Peqlab, Germany), according to the manufacturer’s recommendations. .. The final RNA concentration and quality were determined using a NanoDrop2000 (NanoDrop Technologies, Winooski, Vermont, USA).

Expressing:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: To detect whether paclitaxel treatment alone influences the secretion of cytokines or cytokine expression, real-time reverse transcription polymerase chain reaction was performed. .. Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Clozapine promotes glycolysis and myelin lipid synthesis in cultured oligodendrocytes
Article Snippet: Membrane transporters for lactate and glucose (Figure 1③–④) The expression of MCT1-4 and GLUT1-4 was tested in OLN-93 cells by reverse transcriptase polymerase chain reaction (RT-PCR). .. Total ribonucleic acid (RNA) was isolated from OLN-93 cell cultures using guanidinium isothiocyanate/phenol/chloroform (peqGOLD TriFast™, peqlab, Erlangen, Germany).

Polymerase Chain Reaction:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. Semi-quantitative real-time polymerase chain reaction (PCR) was performed to quantify the mRNA levels of CCL5 in relation to the housekeeping gene β-actin. cDNA samples were amplified with the Power Sybr Green PCR Master Mix (Applied Biosystems) and the respective forward and reverse primers.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. Semiquantitative real-time polymerase chain reaction (PCR) was performed to quantify the messenger RNA (mRNA) levels of IL-6, IL-11, and LIF in relation to the housekeeping gene β-actin.

Article Title: Clozapine promotes glycolysis and myelin lipid synthesis in cultured oligodendrocytes
Article Snippet: Membrane transporters for lactate and glucose (Figure 1③–④) The expression of MCT1-4 and GLUT1-4 was tested in OLN-93 cells by reverse transcriptase polymerase chain reaction (RT-PCR). .. Total ribonucleic acid (RNA) was isolated from OLN-93 cell cultures using guanidinium isothiocyanate/phenol/chloroform (peqGOLD TriFast™, peqlab, Erlangen, Germany).

Software:

Article Title: Influence of Paclitaxel and Heparin on Vitality, Proliferation and Cytokine Production of Endometrial Cancer Cells
Article Snippet: Total ribonucleic acid (RNA) was isolated from endometrial cancer cells using peqGOLD Trifast (Peqlab) and reverse-transcribed using the high capacity cDNA archive kit (Applied Biosystems, Foster City, CA, USA). .. The primers (Invitrogen) were designed using Primer Express primer design software, version 2.0 (Applied Biosystems), with the resulting amplicons having an intron-overlapping sequence.

Article Title: The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1β in vitro
Article Snippet: Total ribonucleic acid (RNA) was isolated from ESCs using PeqGOLD TriFast™ (PeqLab, Erlangen, Germany) and reverse-transcribed using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, USA). .. The primers (Invitrogen) were designed using Primer Express® Primer Design Software v2.0 (Applied Biosystems) with the resulting amplicons having an intron-overlapping sequence.

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  • 95
    PEQLAB quantitative mrna expression analysis qrt pcr total rna
    C-MYC overexpression inhibited chondrogenesis and correlated with increase of hypertrophy. C-MYC-MSC or GFP-MSC were expanded to passage 4, subjected to chondrogenic differentiation for 6 weeks, and induction of chondrogenic markers was evaluated. a Representative images for Safranin O staining in MSC from two selected donors ( n = 4), as indicated; scale bar, 200 μm. b Proteoglycan deposition assessed with DMMB assay; n = 3. c DNA content quantification done with PicoGreen kit; n = 3. d SOX9 <t>mRNA</t> expression measured by <t>qRT-PCR,</t> in relation to GFP control; n = 4. e COL2A1 mRNA expression and the ratio of COL10A1 to COL2A1 mRNAs were analyzed by qRT-PCR; n = 4. *P
    Quantitative Mrna Expression Analysis Qrt Pcr Total Rna, supplied by PEQLAB, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative mrna expression analysis qrt pcr total rna/product/PEQLAB
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative mrna expression analysis qrt pcr total rna - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    PEQLAB analysis total rna
    Demonstration of <t>dsRNA</t> transmission from adult bee to Varroa via the bee hemolymph. Northern blot assay was performed on <t>RNA</t> extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−), pooled RNA extracted from hemolymph collected from bees that had ingested dsRNA-GFP (H+) and from untreated bees (H−), and pooled RNA extracted from Varroa mites parasitizing dsRNA-GFP-treated bees (V+) and untreated bees (V−). C = positive control (GFP-carrying plasmid).
    Analysis Total Rna, supplied by PEQLAB, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/analysis total rna/product/PEQLAB
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    analysis total rna - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    PEQLAB qrt pcr total rna
    NaHS decreases secretion of IL-6, IL-8 and RANTES and diminishes expression of MMP-2 and MMP-14 in IL-1β -stimulated FLS. FLS were either ( A ) treated simultaneously with IL-1β + NaHS or ( B and C ) were pre-treated with NaHS for 1 hr following 1 hr stimulation with IL-1β. PBS and IL-1β-treated FLS were used as negative and positive control, respectively. Cells were further incubated in sulphur-free medium for 1, 3, 6 or 12 hrs. ( A and B ) IL-6 secretion was analysed in cell culture supernatants by ELISA; ( C ) cells were harvested and the expression of MMP-2 and MMP-14 was determined by <t>qRT-PCR.</t> Data were normalised to GAPDH expression. ( D ) For measuring IL-8 and RANTES secretion cells were pre-treated with NaHS or PBS for 1 hr, stimulated with IL-1β for an additional h and incubated in fresh medium for 3 hrs. In all experiments FLS from three different patients were tested three times in duplicates or two times in triplicates. ( A ) * P
    Qrt Pcr Total Rna, supplied by PEQLAB, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr total rna/product/PEQLAB
    Average 94 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    qrt pcr total rna - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    85
    PEQLAB dna microarray total rna
    Effects of S9 mutations in the brz and brb genes on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell <t>RNA</t> after probing with labeled <t>DNA</t> fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brz S9, strain brz S9; brb S9, strain brb S9; brz S9 brb S9, strain brz S9 brb S9. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brzS9 were: 59 ± 4% (OD 600 = 07.-0.8), not detectable (OD 600 = 1.1-1.2) of WT in the dark, and 65 ± 4% (OD 600 = 07.-0.8), 25 ± 1% (OD 600 = 1.1-1.2) in the light; from strain brbS9 they were: 88 ± 4% (OD 600 = 07.-0.8), 119 ± 6% (OD 600 = 1.1-1.2) of WT in the dark, and 135 ± 5% (OD 600 = 07.-0.8), 147 ± 6% (OD 600 = 1.1-1.2) in the light; and from strain brzS9brbS9 they were: 81 ± 5% (OD 600 = 07.-0.8), 83 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 86 ± 5% (OD 600 = 07.-0.8), 115 ± 6% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.
    Dna Microarray Total Rna, supplied by PEQLAB, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray total rna/product/PEQLAB
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    C-MYC overexpression inhibited chondrogenesis and correlated with increase of hypertrophy. C-MYC-MSC or GFP-MSC were expanded to passage 4, subjected to chondrogenic differentiation for 6 weeks, and induction of chondrogenic markers was evaluated. a Representative images for Safranin O staining in MSC from two selected donors ( n = 4), as indicated; scale bar, 200 μm. b Proteoglycan deposition assessed with DMMB assay; n = 3. c DNA content quantification done with PicoGreen kit; n = 3. d SOX9 mRNA expression measured by qRT-PCR, in relation to GFP control; n = 4. e COL2A1 mRNA expression and the ratio of COL10A1 to COL2A1 mRNAs were analyzed by qRT-PCR; n = 4. *P

    Journal: Stem Cell Research & Therapy

    Article Title: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

    doi: 10.1186/s13287-019-1187-z

    Figure Lengend Snippet: C-MYC overexpression inhibited chondrogenesis and correlated with increase of hypertrophy. C-MYC-MSC or GFP-MSC were expanded to passage 4, subjected to chondrogenic differentiation for 6 weeks, and induction of chondrogenic markers was evaluated. a Representative images for Safranin O staining in MSC from two selected donors ( n = 4), as indicated; scale bar, 200 μm. b Proteoglycan deposition assessed with DMMB assay; n = 3. c DNA content quantification done with PicoGreen kit; n = 3. d SOX9 mRNA expression measured by qRT-PCR, in relation to GFP control; n = 4. e COL2A1 mRNA expression and the ratio of COL10A1 to COL2A1 mRNAs were analyzed by qRT-PCR; n = 4. *P

    Article Snippet: RNA extraction and quantitative mRNA expression analysis (qRT-PCR) Total RNA was isolated from pellets using a standard guanidiniumthiocyanate/phenol extraction protocol (peqGOLD TriFastTM; Peqlab, Erlangen, Germany).

    Techniques: Over Expression, Staining, Dimethylmethylene Blue Assay, Expressing, Quantitative RT-PCR

    c-MYC protein accumulation that was increased upon ex vivo passaging. a , b Western blot analysis of c-MYC protein abundancies in different human mesenchymal cell types. Cellular extracts from 25,000 cells for every sample were analyzed by Western blot, with equal volume loading. a c-MYC was assayed in freshly isolated articular chondrocytes (AC), MSC from adipose tissue (ASC), bone marrow MSC (BMSC), and Hela cells served as a positive control for c-MYC protein accumulation. b Bone marrow-derived MSC isolated at passages 0 (P0), 1 (P1), and 2 (P2). c , d c-MYC protein accumulation and mRNA expression were monitored by Western blot ( C ) and qRT-PCR ( n = 3) ( d ) in BMSC at indicated passages (P1, P3, P5, P7, P9, P11), in media with or without basic fibroblast growth factor (bFGF), as indicated ( c ); numbers below WB in C indicate semi-quantitative evaluation of c-MYC protein abundancies normalized to β-actin, as a fold change to a corresponding P1

    Journal: Stem Cell Research & Therapy

    Article Title: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

    doi: 10.1186/s13287-019-1187-z

    Figure Lengend Snippet: c-MYC protein accumulation that was increased upon ex vivo passaging. a , b Western blot analysis of c-MYC protein abundancies in different human mesenchymal cell types. Cellular extracts from 25,000 cells for every sample were analyzed by Western blot, with equal volume loading. a c-MYC was assayed in freshly isolated articular chondrocytes (AC), MSC from adipose tissue (ASC), bone marrow MSC (BMSC), and Hela cells served as a positive control for c-MYC protein accumulation. b Bone marrow-derived MSC isolated at passages 0 (P0), 1 (P1), and 2 (P2). c , d c-MYC protein accumulation and mRNA expression were monitored by Western blot ( C ) and qRT-PCR ( n = 3) ( d ) in BMSC at indicated passages (P1, P3, P5, P7, P9, P11), in media with or without basic fibroblast growth factor (bFGF), as indicated ( c ); numbers below WB in C indicate semi-quantitative evaluation of c-MYC protein abundancies normalized to β-actin, as a fold change to a corresponding P1

    Article Snippet: RNA extraction and quantitative mRNA expression analysis (qRT-PCR) Total RNA was isolated from pellets using a standard guanidiniumthiocyanate/phenol extraction protocol (peqGOLD TriFastTM; Peqlab, Erlangen, Germany).

    Techniques: Ex Vivo, Passaging, Western Blot, Isolation, Positive Control, Derivative Assay, Expressing, Quantitative RT-PCR

    C-MYC overexpression in MSC correlated with its functional activity. Bone marrow-derived MSC transduced with either c-MYC or GFP (control) were expanded and collected at passages 2–4 (early passages) or 9–10 (late passages). a , d , e mRNA expression of indicated genes was measured by qRT-PCR analysis in indicated MSC at early or late passages, as indicated, n = 4; *P

    Journal: Stem Cell Research & Therapy

    Article Title: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

    doi: 10.1186/s13287-019-1187-z

    Figure Lengend Snippet: C-MYC overexpression in MSC correlated with its functional activity. Bone marrow-derived MSC transduced with either c-MYC or GFP (control) were expanded and collected at passages 2–4 (early passages) or 9–10 (late passages). a , d , e mRNA expression of indicated genes was measured by qRT-PCR analysis in indicated MSC at early or late passages, as indicated, n = 4; *P

    Article Snippet: RNA extraction and quantitative mRNA expression analysis (qRT-PCR) Total RNA was isolated from pellets using a standard guanidiniumthiocyanate/phenol extraction protocol (peqGOLD TriFastTM; Peqlab, Erlangen, Germany).

    Techniques: Over Expression, Functional Assay, Activity Assay, Derivative Assay, Transduction, Expressing, Quantitative RT-PCR

    C-MYC overexpression reduced osteogenic and adipogenic markers upon MSC differentiation. a – c c-MYC-MSC or GFP-MSC were expanded to passage 8, and osteogenic differentiation was induced for 3 weeks (D0–D21). a c-MYC mRNA expression measured by qRT-PCR; n = 2. b Total protein content monitored with Bradford reagent at day 14. c Calcium deposition measured by Alizarin Red S staining and normalized to total protein content; d , e C-MYC-MSC or GFP-MSC were expanded to passage 8, and adipogenic differentiation was induced for 3 weeks. d Top: representative images of MSC stained with Oil Red O; scale bar, 100 μm; bottom: quantitative analysis of Oil Red O content; n = 4; *P

    Journal: Stem Cell Research & Therapy

    Article Title: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

    doi: 10.1186/s13287-019-1187-z

    Figure Lengend Snippet: C-MYC overexpression reduced osteogenic and adipogenic markers upon MSC differentiation. a – c c-MYC-MSC or GFP-MSC were expanded to passage 8, and osteogenic differentiation was induced for 3 weeks (D0–D21). a c-MYC mRNA expression measured by qRT-PCR; n = 2. b Total protein content monitored with Bradford reagent at day 14. c Calcium deposition measured by Alizarin Red S staining and normalized to total protein content; d , e C-MYC-MSC or GFP-MSC were expanded to passage 8, and adipogenic differentiation was induced for 3 weeks. d Top: representative images of MSC stained with Oil Red O; scale bar, 100 μm; bottom: quantitative analysis of Oil Red O content; n = 4; *P

    Article Snippet: RNA extraction and quantitative mRNA expression analysis (qRT-PCR) Total RNA was isolated from pellets using a standard guanidiniumthiocyanate/phenol extraction protocol (peqGOLD TriFastTM; Peqlab, Erlangen, Germany).

    Techniques: Over Expression, Expressing, Quantitative RT-PCR, Staining

    Demonstration of dsRNA transmission from adult bee to Varroa via the bee hemolymph. Northern blot assay was performed on RNA extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−), pooled RNA extracted from hemolymph collected from bees that had ingested dsRNA-GFP (H+) and from untreated bees (H−), and pooled RNA extracted from Varroa mites parasitizing dsRNA-GFP-treated bees (V+) and untreated bees (V−). C = positive control (GFP-carrying plasmid).

    Journal: PLoS Pathogens

    Article Title: Bidirectional Transfer of RNAi between Honey Bee and Varroa destructor: Varroa Gene Silencing Reduces Varroa Population

    doi: 10.1371/journal.ppat.1003035

    Figure Lengend Snippet: Demonstration of dsRNA transmission from adult bee to Varroa via the bee hemolymph. Northern blot assay was performed on RNA extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−), pooled RNA extracted from hemolymph collected from bees that had ingested dsRNA-GFP (H+) and from untreated bees (H−), and pooled RNA extracted from Varroa mites parasitizing dsRNA-GFP-treated bees (V+) and untreated bees (V−). C = positive control (GFP-carrying plasmid).

    Article Snippet: RNA extraction and analysis Total RNA for dsRNA-GFP detection experiments was isolated from a single honey bee or from 10 Varroa mites, using phenol-chloroform extraction (peqGOLD Trifast, Peqlab).

    Techniques: Transmission Assay, Northern Blot, Positive Control, Plasmid Preparation

    Silencing of Varroa gene expression following horizontal transfer of dsRNA from bee to Varroa . (A–C) Results (mean ± SE) of real-time RT-PCR of Varroa genes 4, 14 and 9, respectively ( Table S1 ). RNA from Varroa infesting untreated bees, which did not ingest dsRNA, or those that ingested the physiologically inert dsRNA-GFP served as controls. Note that treatment I is devoid of sequence 9. Different letters above columns indicate significant differences between treatments ( P

    Journal: PLoS Pathogens

    Article Title: Bidirectional Transfer of RNAi between Honey Bee and Varroa destructor: Varroa Gene Silencing Reduces Varroa Population

    doi: 10.1371/journal.ppat.1003035

    Figure Lengend Snippet: Silencing of Varroa gene expression following horizontal transfer of dsRNA from bee to Varroa . (A–C) Results (mean ± SE) of real-time RT-PCR of Varroa genes 4, 14 and 9, respectively ( Table S1 ). RNA from Varroa infesting untreated bees, which did not ingest dsRNA, or those that ingested the physiologically inert dsRNA-GFP served as controls. Note that treatment I is devoid of sequence 9. Different letters above columns indicate significant differences between treatments ( P

    Article Snippet: RNA extraction and analysis Total RNA for dsRNA-GFP detection experiments was isolated from a single honey bee or from 10 Varroa mites, using phenol-chloroform extraction (peqGOLD Trifast, Peqlab).

    Techniques: Expressing, Quantitative RT-PCR, Sequencing

    Demonstration of dsRNA transmission from adult bee to Varroa . RT-PCR was performed on RNA extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−). V+ and V− represent amplification of RNA extracted from Varroa parasitizing dsRNA-GFP-treated bees and untreated bees, respectively. M = size markers. C = positive control (GFP-carrying plasmid).

    Journal: PLoS Pathogens

    Article Title: Bidirectional Transfer of RNAi between Honey Bee and Varroa destructor: Varroa Gene Silencing Reduces Varroa Population

    doi: 10.1371/journal.ppat.1003035

    Figure Lengend Snippet: Demonstration of dsRNA transmission from adult bee to Varroa . RT-PCR was performed on RNA extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−). V+ and V− represent amplification of RNA extracted from Varroa parasitizing dsRNA-GFP-treated bees and untreated bees, respectively. M = size markers. C = positive control (GFP-carrying plasmid).

    Article Snippet: RNA extraction and analysis Total RNA for dsRNA-GFP detection experiments was isolated from a single honey bee or from 10 Varroa mites, using phenol-chloroform extraction (peqGOLD Trifast, Peqlab).

    Techniques: Transmission Assay, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Plasmid Preparation

    Demonstration of dsRNA transfer from bee to Varroa and from Varroa to bee. (A) Indirect dsRNA transmission from bee to Varroa parasitizing bee brood. RT-PCR of Varroa -extracted RNA. Numbers represent days from the beginning of dsRNA feeding. M = size markers. C = positive control (GFP-carrying plasmid). + indicates samples collected from dsRNA-GFP-treated hives. – indicates samples collected from untreated, control hives. (B) DsRNA transmission from Varroa to bee. RT-PCR was performed on RNA extracted from a dsRNA-GFP-carrying Varroa (V+) and from Varroa devoid of dsRNA-GFP (V−). B+ represents amplification of RNA from bees infested with dsRNA-GFP-carrying Varroa and B– represents amplification from bees infested with dsRNA-GFP-devoid Varroa . M and C: as in legend for A.

    Journal: PLoS Pathogens

    Article Title: Bidirectional Transfer of RNAi between Honey Bee and Varroa destructor: Varroa Gene Silencing Reduces Varroa Population

    doi: 10.1371/journal.ppat.1003035

    Figure Lengend Snippet: Demonstration of dsRNA transfer from bee to Varroa and from Varroa to bee. (A) Indirect dsRNA transmission from bee to Varroa parasitizing bee brood. RT-PCR of Varroa -extracted RNA. Numbers represent days from the beginning of dsRNA feeding. M = size markers. C = positive control (GFP-carrying plasmid). + indicates samples collected from dsRNA-GFP-treated hives. – indicates samples collected from untreated, control hives. (B) DsRNA transmission from Varroa to bee. RT-PCR was performed on RNA extracted from a dsRNA-GFP-carrying Varroa (V+) and from Varroa devoid of dsRNA-GFP (V−). B+ represents amplification of RNA from bees infested with dsRNA-GFP-carrying Varroa and B– represents amplification from bees infested with dsRNA-GFP-devoid Varroa . M and C: as in legend for A.

    Article Snippet: RNA extraction and analysis Total RNA for dsRNA-GFP detection experiments was isolated from a single honey bee or from 10 Varroa mites, using phenol-chloroform extraction (peqGOLD Trifast, Peqlab).

    Techniques: Transmission Assay, Reverse Transcription Polymerase Chain Reaction, Positive Control, Plasmid Preparation, Amplification

    NaHS decreases secretion of IL-6, IL-8 and RANTES and diminishes expression of MMP-2 and MMP-14 in IL-1β -stimulated FLS. FLS were either ( A ) treated simultaneously with IL-1β + NaHS or ( B and C ) were pre-treated with NaHS for 1 hr following 1 hr stimulation with IL-1β. PBS and IL-1β-treated FLS were used as negative and positive control, respectively. Cells were further incubated in sulphur-free medium for 1, 3, 6 or 12 hrs. ( A and B ) IL-6 secretion was analysed in cell culture supernatants by ELISA; ( C ) cells were harvested and the expression of MMP-2 and MMP-14 was determined by qRT-PCR. Data were normalised to GAPDH expression. ( D ) For measuring IL-8 and RANTES secretion cells were pre-treated with NaHS or PBS for 1 hr, stimulated with IL-1β for an additional h and incubated in fresh medium for 3 hrs. In all experiments FLS from three different patients were tested three times in duplicates or two times in triplicates. ( A ) * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Hydrogen sulphide decreases IL-1β-induced activation of fibroblast-like synoviocytes from patients with osteoarthritis

    doi: 10.1111/jcmm.12405

    Figure Lengend Snippet: NaHS decreases secretion of IL-6, IL-8 and RANTES and diminishes expression of MMP-2 and MMP-14 in IL-1β -stimulated FLS. FLS were either ( A ) treated simultaneously with IL-1β + NaHS or ( B and C ) were pre-treated with NaHS for 1 hr following 1 hr stimulation with IL-1β. PBS and IL-1β-treated FLS were used as negative and positive control, respectively. Cells were further incubated in sulphur-free medium for 1, 3, 6 or 12 hrs. ( A and B ) IL-6 secretion was analysed in cell culture supernatants by ELISA; ( C ) cells were harvested and the expression of MMP-2 and MMP-14 was determined by qRT-PCR. Data were normalised to GAPDH expression. ( D ) For measuring IL-8 and RANTES secretion cells were pre-treated with NaHS or PBS for 1 hr, stimulated with IL-1β for an additional h and incubated in fresh medium for 3 hrs. In all experiments FLS from three different patients were tested three times in duplicates or two times in triplicates. ( A ) * P

    Article Snippet: qRT-PCR Total RNA was isolated from FLS using TriFast (Peqlab, Erlangen, Germany) and reverse transcribed with random hexamers (Finnzymes, Espoo, Finland). qRT-PCR was performed with a DyNAmo SYBR Green qPCR kit (Finnzymes) under the following thermal conditions: 95°C for 7 min. and 40 cycles of 95°C for 20 sec., 60°C for 30 sec. and 72°C for 15 sec. GAPDH was used as a normalizing control.

    Techniques: Expressing, Positive Control, Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    Effects of S9 mutations in the brz and brb genes on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brz S9, strain brz S9; brb S9, strain brb S9; brz S9 brb S9, strain brz S9 brb S9. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brzS9 were: 59 ± 4% (OD 600 = 07.-0.8), not detectable (OD 600 = 1.1-1.2) of WT in the dark, and 65 ± 4% (OD 600 = 07.-0.8), 25 ± 1% (OD 600 = 1.1-1.2) in the light; from strain brbS9 they were: 88 ± 4% (OD 600 = 07.-0.8), 119 ± 6% (OD 600 = 1.1-1.2) of WT in the dark, and 135 ± 5% (OD 600 = 07.-0.8), 147 ± 6% (OD 600 = 1.1-1.2) in the light; and from strain brzS9brbS9 they were: 81 ± 5% (OD 600 = 07.-0.8), 83 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 86 ± 5% (OD 600 = 07.-0.8), 115 ± 6% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Journal: BMC Molecular Biology

    Article Title: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

    doi: 10.1186/1471-2199-12-42

    Figure Lengend Snippet: Effects of S9 mutations in the brz and brb genes on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brz S9, strain brz S9; brb S9, strain brb S9; brz S9 brb S9, strain brz S9 brb S9. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brzS9 were: 59 ± 4% (OD 600 = 07.-0.8), not detectable (OD 600 = 1.1-1.2) of WT in the dark, and 65 ± 4% (OD 600 = 07.-0.8), 25 ± 1% (OD 600 = 1.1-1.2) in the light; from strain brbS9 they were: 88 ± 4% (OD 600 = 07.-0.8), 119 ± 6% (OD 600 = 1.1-1.2) of WT in the dark, and 135 ± 5% (OD 600 = 07.-0.8), 147 ± 6% (OD 600 = 1.1-1.2) in the light; and from strain brzS9brbS9 they were: 81 ± 5% (OD 600 = 07.-0.8), 83 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 86 ± 5% (OD 600 = 07.-0.8), 115 ± 6% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Article Snippet: DNA-Microarray Total RNA was prepared using peqGold RNAPure solution (Peqlab Biotechnology) from cells grown to an OD600 of 0.5.

    Techniques: Agarose Gel Electrophoresis, Staining, Northern Blot, Labeling

    Effects of CBD domain insertion in the brb gene on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brb CBD, brb CBD gene fusion strain. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from the brb CBD mutant corresponded to 67 ± 2% (OD 600 = 07.-0.8), 70 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 85 ± 3% (OD 600 = 07.-0.8), 85 ± 2% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Journal: BMC Molecular Biology

    Article Title: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

    doi: 10.1186/1471-2199-12-42

    Figure Lengend Snippet: Effects of CBD domain insertion in the brb gene on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brb CBD, brb CBD gene fusion strain. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from the brb CBD mutant corresponded to 67 ± 2% (OD 600 = 07.-0.8), 70 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 85 ± 3% (OD 600 = 07.-0.8), 85 ± 2% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Article Snippet: DNA-Microarray Total RNA was prepared using peqGold RNAPure solution (Peqlab Biotechnology) from cells grown to an OD600 of 0.5.

    Techniques: Agarose Gel Electrophoresis, Staining, Northern Blot, Labeling, Mutagenesis

    Effects of inactivation of the brb gene translation on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brb stop1, strain brb stop1; brb stop2, strain brb stop2. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brb stop1 were: 117 ± 5% (OD 600 = 07.-0.8), 86 ± 4% (OD 600 = 1.1-1.2) of WT in the dark, and 106 ± 2% (OD 600 = 07.-0.8), 132 ± 9% (OD 600 = 1.1-1.2) in the light; from strain brb stop2 they were: 175 ± 6% (OD 600 = 07.-0.8), 95 ± 4% (OD 600 = 1.1-1.2) of WT in the dark, and 124 ± 9% (OD 600 = 07.-0.8), 118 ± 11% (OD 600 = 1.1-1.2) in the light. Panel B shows locations of the mutations in the brz and brb genes.

    Journal: BMC Molecular Biology

    Article Title: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

    doi: 10.1186/1471-2199-12-42

    Figure Lengend Snippet: Effects of inactivation of the brb gene translation on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brb stop1, strain brb stop1; brb stop2, strain brb stop2. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brb stop1 were: 117 ± 5% (OD 600 = 07.-0.8), 86 ± 4% (OD 600 = 1.1-1.2) of WT in the dark, and 106 ± 2% (OD 600 = 07.-0.8), 132 ± 9% (OD 600 = 1.1-1.2) in the light; from strain brb stop2 they were: 175 ± 6% (OD 600 = 07.-0.8), 95 ± 4% (OD 600 = 1.1-1.2) of WT in the dark, and 124 ± 9% (OD 600 = 07.-0.8), 118 ± 11% (OD 600 = 1.1-1.2) in the light. Panel B shows locations of the mutations in the brz and brb genes.

    Article Snippet: DNA-Microarray Total RNA was prepared using peqGold RNAPure solution (Peqlab Biotechnology) from cells grown to an OD600 of 0.5.

    Techniques: Agarose Gel Electrophoresis, Staining, Northern Blot, Labeling

    Effects of M1 and M2 mutations in the brb genes on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brz S9, strain brz S9; brb M1, strain brb M1; brz S9 brb M2, strain brz S9 brb M2. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brzS9 were: 58 ± 1% (OD 600 = 07.-0.8), 20 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 46 ± 3% (OD 600 = 07.-0.8), 10 ± 1% (OD 600 = 1.1-1.2) in the light; from strain brbM1 they were: 95 ± 2% (OD 600 = 07.-0.8), 129 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 82 ± 2% (OD 600 = 07.-0.8), 105 ± 7% (OD 600 = 1.1-1.2) in the light; and from the brzS9brbM2 they were: 74 ± 2% (OD 600 = 07.-0.8), 47 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 64 ± 3% (OD 600 = 07.-0.8), 33 ± 3% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Journal: BMC Molecular Biology

    Article Title: A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

    doi: 10.1186/1471-2199-12-42

    Figure Lengend Snippet: Effects of M1 and M2 mutations in the brb genes on bop transcription . In panel A, the upper blocks represent the 16S rRNA bands on the agarose gel stained by ethidium bromide. The lower blocks show northern blots of total cell RNA after probing with labeled DNA fragments containing the bop gene. Cells were grown in the dark and light. Culture OD 600 at which RNA was extracted is given at right. WT R1, wild-type strain R1; brz S9, strain brz S9; brb M1, strain brb M1; brz S9 brb M2, strain brz S9 brb M2. According to densitometry, the levels of bop mRNA (normalized to 16S rRNA) from strain brzS9 were: 58 ± 1% (OD 600 = 07.-0.8), 20 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 46 ± 3% (OD 600 = 07.-0.8), 10 ± 1% (OD 600 = 1.1-1.2) in the light; from strain brbM1 they were: 95 ± 2% (OD 600 = 07.-0.8), 129 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 82 ± 2% (OD 600 = 07.-0.8), 105 ± 7% (OD 600 = 1.1-1.2) in the light; and from the brzS9brbM2 they were: 74 ± 2% (OD 600 = 07.-0.8), 47 ± 2% (OD 600 = 1.1-1.2) of WT in the dark, and 64 ± 3% (OD 600 = 07.-0.8), 33 ± 3% (OD 600 = 1.1-1.2) in the light. Panel B shows the locations of mutations in the brz and brb genes.

    Article Snippet: DNA-Microarray Total RNA was prepared using peqGold RNAPure solution (Peqlab Biotechnology) from cells grown to an OD600 of 0.5.

    Techniques: Agarose Gel Electrophoresis, Staining, Northern Blot, Labeling