total genomic dna input  (Millipore)


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    Genomic DNA
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    Catalog Number:
    irmm447
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    Structured Review

    Millipore total genomic dna input

    https://www.bioz.com/result/total genomic dna input/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total genomic dna input - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Amplification:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Agarose Gel Electrophoresis:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Expressing:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Southern Blot:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Methylation:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Isolation:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Purification:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Mouse Assay:

    Article Title: A NOVEL, DIRECT NO DONOR REGULATES OSTEOBLAST AND OSTEOCLAST FUNCTIONS AND INCREASES BONE MASS IN OVARIECTOMIZED MICE
    Article Snippet: .. To generate PRKG2f/f mice, we PCR-amplified genomic DNA fragments encoding prkg2 exon III with flanking sequences from 129/SvJ ES cells using KOD polymerase (EMD Millipore Corporation). .. The prkg2 -floxed construct was assembled in the vector pDLNL (gift from Ju Chen of UCSD) and consisted of a 4.2 kb 5′ arm, a 0.55 kb fragment including exon III flanked by LoxP sites, a 1.3 kb neo cassette flanked by FRT sites, and a 3kb 3′ arm.

    Polymerase Chain Reaction:

    Article Title: A NOVEL, DIRECT NO DONOR REGULATES OSTEOBLAST AND OSTEOCLAST FUNCTIONS AND INCREASES BONE MASS IN OVARIECTOMIZED MICE
    Article Snippet: .. To generate PRKG2f/f mice, we PCR-amplified genomic DNA fragments encoding prkg2 exon III with flanking sequences from 129/SvJ ES cells using KOD polymerase (EMD Millipore Corporation). .. The prkg2 -floxed construct was assembled in the vector pDLNL (gift from Ju Chen of UCSD) and consisted of a 4.2 kb 5′ arm, a 0.55 kb fragment including exon III flanked by LoxP sites, a 1.3 kb neo cassette flanked by FRT sites, and a 3kb 3′ arm.

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Article Title: A refined Panax ginseng karyotype based on an ultra-high copy 167-bp tandem repeat and ribosomal DNAs
    Article Snippet: .. The PCR mixture (total volume of 50 μL) consisted of 2.5 U ExTaq DNA polymerase, 5 μL 10× ExTaq buffer, 2.5mM each dNTP (RR001A; TaKaRa Bio Inc., Kusatsu, Shiga, Japan), 0.3μM each primer, < 500 ng BAC PgH005J07 or P. ginseng gDNA template, and DNase-free water (W4502; Sigma–Aldrich, St. Louis, MO, USA). ..

    Incubation:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Positron Emission Tomography:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    BAC Assay:

    Article Title: A refined Panax ginseng karyotype based on an ultra-high copy 167-bp tandem repeat and ribosomal DNAs
    Article Snippet: .. The PCR mixture (total volume of 50 μL) consisted of 2.5 U ExTaq DNA polymerase, 5 μL 10× ExTaq buffer, 2.5mM each dNTP (RR001A; TaKaRa Bio Inc., Kusatsu, Shiga, Japan), 0.3μM each primer, < 500 ng BAC PgH005J07 or P. ginseng gDNA template, and DNase-free water (W4502; Sigma–Aldrich, St. Louis, MO, USA). ..

    Modification:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Transformation Assay:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Recombinant:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Plasmid Preparation:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

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  • 99
    Millipore total genomic dna input
    Total Genomic Dna Input, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total genomic dna input/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    total genomic dna input - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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