torula type ix yeast rna  (Millipore)


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    Name:
    Ribonucleic acid diethylaminoethanol salt
    Description:

    Catalog Number:
    r3629
    Price:
    None
    Applications:
    Ribonucleic acid (RNA) from torula yeast may be used as a substrate for studying ribonuclease activities of enzymes such as ribonuclease-A, ribonuclease T1 (RNAase) and bougainvillea xbuttiana antiviral protein 1 (BBAP1).
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    Structured Review

    Millipore torula type ix yeast rna

    https://www.bioz.com/result/torula type ix yeast rna/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    torula type ix yeast rna - by Bioz Stars, 2020-07
    91/100 stars

    Related Products / Commonly Used Together

    hybridization buffer

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    Related Articles

    Concentration Assay:

    Article Title: RNA-binding protein QKI-5 inhibits the proliferation of clear cell renal cell carcinoma via post-transcriptional stabilization of RASA1 mRNA
    Article Snippet: .. RNase (Sigma, R3629) was added at a final concentration of 2 mg/ml, and the cells were incubated at 37°C for 15 min, followed by incubation in 15 mg/ml propidium iodide (PI, Sigma, P4170) for 15 min at room temperature. .. At least 10000 cells per sample were collected and analyzed using a flow cytometer (Beckman Coulter, Cytomics FC500).

    Article Title: RASSF6 promotes p21Cip1/Waf1-dependent cell cycle arrest and apoptosis through activation of the JNK/SAPK pathway in clear cell renal cell carcinoma
    Article Snippet: .. RNase (Sigma, R3629) was added at a final concentration of 2 mg/ml, and the cells were incubated at 37 °C for 15 min, followed by incubation in 15 mg/ml propidium iodide (PI, Sigma, P4170) for 15 min at room temperature. .. At least 10 000 cells per sample were collected and analyzed on a flow cytometer (Beckman Coulter, Cytomics FC 500).

    Incubation:

    Article Title: Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus
    Article Snippet: .. Embryos were then incubated in hybridization buffer [50% formamide, 5× SSC (750 mM NaCl, 75 mM sodium citrate, pH 7.0), 1 mg/mL torula type IX yeast RNA (R3629-25G; Sigma-Aldrich, St. Louis, MO), 1× Denhardt’s solution [0.02% BSA, 0.02% Ficoll, 0.02% polyvinylpyrrolidone (PVP), 10 mM EDTA, 0.1% SDS, 100 μg/mL heparin, 0.1% Tween 20] for 3 h at 65°C. .. Riboprobes for the gene spib.a , which encodes an ETS transcription factor expressed in primitive myeloid cells in the anterior ventral blood island (aVBI), and the myeloperoxidase gene ( mpo ), which encodes a peroxidase produced by neutrophilic granulocytes arising in the aVBI, were synthesized with digoxygenin-11-UTP (DIG) and fluorescein-12-UTP (FL) labels (Roche Diagnostics, Indianapolis, IN), respectively ( ).

    Article Title: RNA-binding protein QKI-5 inhibits the proliferation of clear cell renal cell carcinoma via post-transcriptional stabilization of RASA1 mRNA
    Article Snippet: .. RNase (Sigma, R3629) was added at a final concentration of 2 mg/ml, and the cells were incubated at 37°C for 15 min, followed by incubation in 15 mg/ml propidium iodide (PI, Sigma, P4170) for 15 min at room temperature. .. At least 10000 cells per sample were collected and analyzed using a flow cytometer (Beckman Coulter, Cytomics FC500).

    Article Title: RASSF6 promotes p21Cip1/Waf1-dependent cell cycle arrest and apoptosis through activation of the JNK/SAPK pathway in clear cell renal cell carcinoma
    Article Snippet: .. RNase (Sigma, R3629) was added at a final concentration of 2 mg/ml, and the cells were incubated at 37 °C for 15 min, followed by incubation in 15 mg/ml propidium iodide (PI, Sigma, P4170) for 15 min at room temperature. .. At least 10 000 cells per sample were collected and analyzed on a flow cytometer (Beckman Coulter, Cytomics FC 500).

    Hybridization:

    Article Title: Two-color fluorescence in situ hybridization using chromogenic substrates in Xenopus
    Article Snippet: .. Embryos were then incubated in hybridization buffer [50% formamide, 5× SSC (750 mM NaCl, 75 mM sodium citrate, pH 7.0), 1 mg/mL torula type IX yeast RNA (R3629-25G; Sigma-Aldrich, St. Louis, MO), 1× Denhardt’s solution [0.02% BSA, 0.02% Ficoll, 0.02% polyvinylpyrrolidone (PVP), 10 mM EDTA, 0.1% SDS, 100 μg/mL heparin, 0.1% Tween 20] for 3 h at 65°C. .. Riboprobes for the gene spib.a , which encodes an ETS transcription factor expressed in primitive myeloid cells in the anterior ventral blood island (aVBI), and the myeloperoxidase gene ( mpo ), which encodes a peroxidase produced by neutrophilic granulocytes arising in the aVBI, were synthesized with digoxygenin-11-UTP (DIG) and fluorescein-12-UTP (FL) labels (Roche Diagnostics, Indianapolis, IN), respectively ( ).

    Article Title: Differentiation of human pluripotent stem cells into colonic organoids via transient activation of BMP signaling
    Article Snippet: .. Embryos were then re-fixed in PBS+3.7% formaldehyde for 20 minutes, and pre-hybridized in hybridization buffer (50% Formamide, 5X SSC pH7,1 mg/mL Torula Yeast RNA (Sigma R3629-5G), 100 ug/mL heparin (Sigma H3393-50KU) , 1X Denhardt’s , 0.1% Tween-20, 10mM EDTA pH 8) for 3 hours at 65°C. .. Embryos were then incubated in DIG-probe solution (0.5ug/mL) overnight at 65°C, washed extensively in 2X and 0.2X SSC buffers, blocked in blocking buffer (maleic acid buffer, MAB, 100mM maleic acid,150mM NaCl, 20% Heat-inactivated lamb serum (Gibco 16070-096), 2% Roche Blocking reagent (Sigma 11-096-176-001)) for 2 hours at room temperature, and incubated in anti-DIG-alkaline phosphatase (Sigma11093274910) diluted 1:5,000 in blocking buffer overnight at 4°C.

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