topo xl cloning kit  (Thermo Fisher)


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    Name:
    TOPO TA Cloning Kit
    Description:
    TOPO TA Cloning Dual Promoter kits are for fast efficient cloning and subsequent in vitro transcription translation These kits includes the pCR II TOPO TA vector with dual T7 and SP6 promoters By eliminating time consuming and tedious restriction site cloning TOPO cloning is the most reliable cloning method featuring a 5 minute cloning reaction adn 3 step protocol and yielding up to 95 recombinants Convenient features of this TOPO TA Cloning Dual Promoter kit include • 3 T overhangs for direct ligation of Taq amplified PCR products • T7 and SP6 promoters for efficient in vitro transcription • M13 forward and reverse primer sites for sequencing • 16 convenient restriction sites including EcoRI flanking your insert for subsequent excision or subcloning • Kanamycin and ampicillin resistance for your choice of selection in E coli • Easy blue white colony screening for selection of recombinants Kit Options TOPO TA Cloning Dual Promoter Kits TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs • General cloning TOP10 cells Cat Nos K4600 J10 K4600 01 K4600 40 • High efficiency cloning TOP10 Electrocomp cells Cat Nos K4660 01 K4660 40 • General cloning bacteriophage T1 resistance DH5α T1R cells Cat Nos K4620 01 K4620 40 • Fast growth Mach1 T1R chemically competent E coli Cat No K4610 20 • Repressor induction needs TOP10F cells Cat Nos K4650 01 K4650 40 • Provide your own cells Cat Nos 450640 and 452640
    Catalog Number:
    450640
    Price:
    None
    Applications:
    Cloning|PCR Cloning
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher topo xl cloning kit
    Summary of the strategy adopted to obtain the M. persicae RyR cDNA sequence. Five overlapping fragments (F1a/b, F2, F3 and <t>F4)</t> were initially PCR amplified. Fragments F1a, F1b, F2 and F3 were sub-cloned into pJET1.2blunt vector (Thermo-Fermentas) and fragment F4 was sub-cloned into a <t>TOPO®TA</t> vector (Invitrogen) for sequencing.
    TOPO TA Cloning Dual Promoter kits are for fast efficient cloning and subsequent in vitro transcription translation These kits includes the pCR II TOPO TA vector with dual T7 and SP6 promoters By eliminating time consuming and tedious restriction site cloning TOPO cloning is the most reliable cloning method featuring a 5 minute cloning reaction adn 3 step protocol and yielding up to 95 recombinants Convenient features of this TOPO TA Cloning Dual Promoter kit include • 3 T overhangs for direct ligation of Taq amplified PCR products • T7 and SP6 promoters for efficient in vitro transcription • M13 forward and reverse primer sites for sequencing • 16 convenient restriction sites including EcoRI flanking your insert for subsequent excision or subcloning • Kanamycin and ampicillin resistance for your choice of selection in E coli • Easy blue white colony screening for selection of recombinants Kit Options TOPO TA Cloning Dual Promoter Kits TOPO TA Cloning Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs • General cloning TOP10 cells Cat Nos K4600 J10 K4600 01 K4600 40 • High efficiency cloning TOP10 Electrocomp cells Cat Nos K4660 01 K4660 40 • General cloning bacteriophage T1 resistance DH5α T1R cells Cat Nos K4620 01 K4620 40 • Fast growth Mach1 T1R chemically competent E coli Cat No K4610 20 • Repressor induction needs TOP10F cells Cat Nos K4650 01 K4650 40 • Provide your own cells Cat Nos 450640 and 452640
    https://www.bioz.com/result/topo xl cloning kit/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    topo xl cloning kit - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Molecular cloning, characterisation and mRNA expression of the ryanodine receptor from the peach-potato aphid, Myzus persicae"

    Article Title: Molecular cloning, characterisation and mRNA expression of the ryanodine receptor from the peach-potato aphid, Myzus persicae

    Journal: Gene

    doi: 10.1016/j.gene.2014.11.035

    Summary of the strategy adopted to obtain the M. persicae RyR cDNA sequence. Five overlapping fragments (F1a/b, F2, F3 and F4) were initially PCR amplified. Fragments F1a, F1b, F2 and F3 were sub-cloned into pJET1.2blunt vector (Thermo-Fermentas) and fragment F4 was sub-cloned into a TOPO®TA vector (Invitrogen) for sequencing.
    Figure Legend Snippet: Summary of the strategy adopted to obtain the M. persicae RyR cDNA sequence. Five overlapping fragments (F1a/b, F2, F3 and F4) were initially PCR amplified. Fragments F1a, F1b, F2 and F3 were sub-cloned into pJET1.2blunt vector (Thermo-Fermentas) and fragment F4 was sub-cloned into a TOPO®TA vector (Invitrogen) for sequencing.

    Techniques Used: Sequencing, Polymerase Chain Reaction, Amplification, Clone Assay, Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Article Title: A Hypomorphic Cystathionine ß-Synthase Gene Contributes to Cavefish Eye Loss by Disrupting Optic Vasculature
    Article Snippet: .. The PCR products were cloned into the TOPO vector using the TOPO TA Cloning Kit Dual Promotor (Life Technologies) and confirmed by sequencing. .. Sense and anti-sense DIG-labeled RNA probes were prepared with SP6 RNA and T7 RNA Polymerases (Roche Diagnostics, Indianapolis, IN).

    Article Title: Anaerobic Metabolism of Catechol by the Denitrifying Bacterium Thauera aromatica--a Result of Promiscuous Enzymes and Regulators? ▿
    Article Snippet: .. The 3-hydroxybenzoate-CoA ligase gene was cloned into the vector pEX5-CT/TOPO with a TOPO cloning kit from Invitrogen (Karlsruhe, Germany). .. The ligation mixture (1 μl of vector [10 ng of DNA], 1 μl of 6× ligation buffer, 4 μl of purified PCR product [50 ng of DNA]) was incubated at room temperature for 20 min. One microliter of the TOPO ligation reaction mixture was added to 200 μl of competent cells ( ) that were kept on ice for 10 min.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Article Title: Epigenetic and Genetic Alterations in Netrin-1 Receptors UNC5C and DCC in Human Colon Cancer
    Article Snippet: .. Each of the PCR products subsequently was cloned using the TOPO-TA cloning system (Invitrogen Life Technologies Inc). .. Nine to 11 white colonies indicating positive clones with PCR products were sequenced using ABI PRISM Big Dye Terminator v1.1 Cycle Sequencing Kits on an ABI PRISM 3100 Avant Genetic analyzer (Applied Biosystems, Foster City, CA).

    Amplification:

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    TA Cloning:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: A Hypomorphic Cystathionine ß-Synthase Gene Contributes to Cavefish Eye Loss by Disrupting Optic Vasculature
    Article Snippet: .. The PCR products were cloned into the TOPO vector using the TOPO TA Cloning Kit Dual Promotor (Life Technologies) and confirmed by sequencing. .. Sense and anti-sense DIG-labeled RNA probes were prepared with SP6 RNA and T7 RNA Polymerases (Roche Diagnostics, Indianapolis, IN).

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Purification:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Sequencing:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: A Hypomorphic Cystathionine ß-Synthase Gene Contributes to Cavefish Eye Loss by Disrupting Optic Vasculature
    Article Snippet: .. The PCR products were cloned into the TOPO vector using the TOPO TA Cloning Kit Dual Promotor (Life Technologies) and confirmed by sequencing. .. Sense and anti-sense DIG-labeled RNA probes were prepared with SP6 RNA and T7 RNA Polymerases (Roche Diagnostics, Indianapolis, IN).

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Polymerase Chain Reaction:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: A Hypomorphic Cystathionine ß-Synthase Gene Contributes to Cavefish Eye Loss by Disrupting Optic Vasculature
    Article Snippet: .. The PCR products were cloned into the TOPO vector using the TOPO TA Cloning Kit Dual Promotor (Life Technologies) and confirmed by sequencing. .. Sense and anti-sense DIG-labeled RNA probes were prepared with SP6 RNA and T7 RNA Polymerases (Roche Diagnostics, Indianapolis, IN).

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Article Title: Epigenetic and Genetic Alterations in Netrin-1 Receptors UNC5C and DCC in Human Colon Cancer
    Article Snippet: .. Each of the PCR products subsequently was cloned using the TOPO-TA cloning system (Invitrogen Life Technologies Inc). .. Nine to 11 white colonies indicating positive clones with PCR products were sequenced using ABI PRISM Big Dye Terminator v1.1 Cycle Sequencing Kits on an ABI PRISM 3100 Avant Genetic analyzer (Applied Biosystems, Foster City, CA).

    Transformation Assay:

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Plasmid Preparation:

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Article Title: A Hypomorphic Cystathionine ß-Synthase Gene Contributes to Cavefish Eye Loss by Disrupting Optic Vasculature
    Article Snippet: .. The PCR products were cloned into the TOPO vector using the TOPO TA Cloning Kit Dual Promotor (Life Technologies) and confirmed by sequencing. .. Sense and anti-sense DIG-labeled RNA probes were prepared with SP6 RNA and T7 RNA Polymerases (Roche Diagnostics, Indianapolis, IN).

    Article Title: Anaerobic Metabolism of Catechol by the Denitrifying Bacterium Thauera aromatica--a Result of Promiscuous Enzymes and Regulators? ▿
    Article Snippet: .. The 3-hydroxybenzoate-CoA ligase gene was cloned into the vector pEX5-CT/TOPO with a TOPO cloning kit from Invitrogen (Karlsruhe, Germany). .. The ligation mixture (1 μl of vector [10 ng of DNA], 1 μl of 6× ligation buffer, 4 μl of purified PCR product [50 ng of DNA]) was incubated at room temperature for 20 min. One microliter of the TOPO ligation reaction mixture was added to 200 μl of competent cells ( ) that were kept on ice for 10 min.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

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  • 99
    Thermo Fisher topo xl cloning kit
    Topo Xl Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo xl cloning kit/product/Thermo Fisher
    Average 99 stars, based on 313 article reviews
    Price from $9.99 to $1999.99
    topo xl cloning kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Thermo Fisher topo xl pcr cloning kit
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    Price from $9.99 to $1999.99
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