topo cloning strategy  (Thermo Fisher)


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    Structured Review

    Thermo Fisher topo cloning strategy
    Topo Cloning Strategy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo cloning strategy/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    topo cloning strategy - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
    Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

    Clone Assay:

    Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
    Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

    Plasmid Preparation:

    Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
    Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

    Article Title: The Mitochondrial Translocator Protein, TSPO, Inhibits HIV-1 Envelope Glycoprotein Biosynthesis via the Endoplasmic Reticulum-Associated Protein Degradation Pathway
    Article Snippet: .. The TSPO gene was then subcloned into the pcDNA3.1/V5-His-TOPO vector by a TOPO-cloning strategy (Invitrogen). .. The pcDNA3.3-TOPO vector expressing human codon-optimized Cas9 was obtained from the G. M. Church laboratory through Addgene ( ).

    Expressing:

    Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
    Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

    Construct:

    Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
    Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

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    Thermo Fisher gateway cloning strategy
    Gateway Cloning Strategy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway cloning strategy/product/Thermo Fisher
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gateway cloning strategy - by Bioz Stars, 2020-09
    90/100 stars
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