top10 supercompetent cells  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Thermo Fisher top10 supercompetent cells
    Top10 Supercompetent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 supercompetent cells/product/Thermo Fisher
    Average 96 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    top10 supercompetent cells - by Bioz Stars, 2020-04
    96/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: Platinum Pfx DNA polymerase (Invitrogen) was used for DNA amplification with a RoboCycler Gradient 96 Temperature Cycler (Stratagene). .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: Platinum Pfx DNA polymerase (Invitrogen) was used for DNA amplification with a RoboCycler Gradient 96 Temperature Cycler (Stratagene). .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Ligation:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Mutagenesis:

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: Single missense mutations p.Cys1099Tyr, p.Cys2771Arg, p.Cys2773Arg and p.Cys2811Ala were inserted into the same vector by site-directed mutagenesis employing the QuikChange kit (Stratagene) and the resulting vectors were sequenced to confirm insertion of the mutations and absence of any additional unwanted mutations. .. Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN).

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: .. The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. Plasmids were purified by the Endofree Plasmid Maxi Kit (QIAGEN).

    Isolation:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: After digestion, the fragments were isolated from a 0.8 % NuSieve GTG agarose (ISC BioExpress) tris-borate-EDTA gel using a Qiagen, Qiaex II Gel Extraction Kit. .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: After digestion, the fragments were isolated from a 0.8 % NuSieve GTG agarose (ISC BioExpress) tris-borate-EDTA gel using a Qiagen, Qiaex II Gel Extraction Kit. .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Polymerase Chain Reaction:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The PCR products were cleaned using Wizard PCR Preps (Promega). .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The PCR products were cleaned using Wizard PCR Preps (Promega). .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    TA Cloning:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Cell Culture:

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: Paragraph title: Cell culture and VWF expression in HEK293 cells ... Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN).

    Purification:

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: .. Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN). .. 4 μg vector DNA were used to transfect HEK293 cells (2*106 ) employing Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. Plasmids were purified by the Endofree Plasmid Maxi Kit (QIAGEN).

    Sequencing:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. .. The sequences around Xho I and BsiW I were confirmed by sequencing (Lone Star Labs, Inc.), and the plasmid was named pSCP102.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. .. The sequences around Xho I and BsiW I were confirmed by sequencing (Lone Star Labs, Inc.), and the plasmid was named pSCP102.

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. The human VWF propeptide (VWFpp), propeptide-deleted VWF (Δpro), and myc-his–tagged wild-type VWF expression vectors were constructed as previously described., Briefly, pCIneo-VWFpp expression vectors contain signal peptide and VWFpp sequence followed by a stop codon with mature VWF sequence deleted.

    Selection:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. .. After selection, plasmid was isolated from LB broth cultures and screened with Hpa I.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. .. After selection, plasmid was isolated from LB broth cultures and screened with Hpa I.

    Construct:

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. The human VWF propeptide (VWFpp), propeptide-deleted VWF (Δpro), and myc-his–tagged wild-type VWF expression vectors were constructed as previously described., Briefly, pCIneo-VWFpp expression vectors contain signal peptide and VWFpp sequence followed by a stop codon with mature VWF sequence deleted.

    Expressing:

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: Paragraph title: Cell culture and VWF expression in HEK293 cells ... Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN).

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: .. The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. Plasmids were purified by the Endofree Plasmid Maxi Kit (QIAGEN).

    Modification:

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN). .. The cells were selected for stable expression for 2 weeks by adding 500 μg/ml G418 (Thermo Fisher Scientific) to the Dulbecco modified Eagle medium (Thermo Fisher Scientific) with 10% [vol/vol] fetal bovine serum.

    Gel Extraction:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: After digestion, the fragments were isolated from a 0.8 % NuSieve GTG agarose (ISC BioExpress) tris-borate-EDTA gel using a Qiagen, Qiaex II Gel Extraction Kit. .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: After digestion, the fragments were isolated from a 0.8 % NuSieve GTG agarose (ISC BioExpress) tris-borate-EDTA gel using a Qiagen, Qiaex II Gel Extraction Kit. .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Transformation Assay:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: .. The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit. ..

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: .. Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN). .. 4 μg vector DNA were used to transfect HEK293 cells (2*106 ) employing Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Recombinant:

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: VWF mutagenesis and recombinant expression of the mutation identified in the patients were performed as described previously. .. The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen).

    Plasmid Preparation:

    Article Title: Environmentally-Modulated Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics
    Article Snippet: Paragraph title: Plasmid construction for main experiments ... The ligation reaction was transformed into TOP10 SuperCompetent Cells (Invitrogen) using the “One-Shot Transformation Reaction” procedure in TOPO TA Cloning Kit.

    Article Title: Advancing multimer analysis of von Willebrand factor by single-molecule AFM imaging
    Article Snippet: .. Top10 supercompetent cells (Invitrogen) were transformed with these vectors and plasmid purification was performed using the Endofree Plasmid Maxi Kit (QIAGEN). .. 4 μg vector DNA were used to transfect HEK293 cells (2*106 ) employing Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.

    Article Title: The mutation N528S in the von Willebrand factor (VWF) propeptide causes defective multimerization and storage of VWF
    Article Snippet: .. The expression vector pRC.CMV containing full-length mutant and wild-type (wt) VWF cDNA was used to transform Top10 supercompetent cells (Invitrogen). .. Plasmids were purified by the Endofree Plasmid Maxi Kit (QIAGEN).