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Thermo Fisher top10 f
Top10 F, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/top10 f/product/Thermo Fisher
Average 88 stars, based on 6 article reviews
Price from $9.99 to $1999.99
top10 f - by Bioz Stars, 2020-07
88/100 stars

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Related Articles

Clone Assay:

Article Title: Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap
Article Snippet: .. The separated fragment was ligated into the p-Tet-On vector by using T4 DNA Ligase (invitrogen, USA), Ligation reaction was transformed into the Top10 f (Invitrogen, USA) by heat shock method and the accuracy of clones was confirmed by double digestion. ..

Article Title: Discovery of Calcium as a Biofilm-Promoting Signal for Vibrio fischeri Reveals New Phenotypes and Underlying Regulatory Complexity
Article Snippet: .. A variety of E. coli host strains, including GT115 (Invivogen, San Diego, CA), CC118 λ pir , TAM1 or TAM1 λ pir (Active Motif, Carlsbad, CA), DH5α , DH5α λ pir , Top10 F′ (Invitrogen, now Thermo Fisher Scientific), S17-1 λ pir , and π3813 , were used for the purposes of cloning, plasmid maintenance, and conjugation. ..

Article Title: Multiple factors contribute to keeping levels of the symbiosis regulator RscS low
Article Snippet: .. In addition to the V. fischeri strains, the following strains of Escherichia coli were used for the purposes of cloning and conjugation: DH5α , TOP10 F′ (Invitrogen, Carlsbad, CA) and Tam 1 (Active Motif, Carlsbad, CA). .. Most plasmids were constructed using standard molecular biology techniques with restriction enzymes or polymerase chain reaction.

Article Title: Inhibition of SypG-Induced Biofilms and Host Colonization by the Negative Regulator SypE in Vibrio fischeri
Article Snippet: .. E. coli strains TAM1 (Active Motif, Carlsbad, CA), TOP10 F (Invitrogen, Carlsbad, CA), and GT115 (InvivoGen, San Diego, CA) were used for cloning. ..

Conjugation Assay:

Article Title: Discovery of Calcium as a Biofilm-Promoting Signal for Vibrio fischeri Reveals New Phenotypes and Underlying Regulatory Complexity
Article Snippet: .. A variety of E. coli host strains, including GT115 (Invivogen, San Diego, CA), CC118 λ pir , TAM1 or TAM1 λ pir (Active Motif, Carlsbad, CA), DH5α , DH5α λ pir , Top10 F′ (Invitrogen, now Thermo Fisher Scientific), S17-1 λ pir , and π3813 , were used for the purposes of cloning, plasmid maintenance, and conjugation. ..

Article Title: Multiple factors contribute to keeping levels of the symbiosis regulator RscS low
Article Snippet: .. In addition to the V. fischeri strains, the following strains of Escherichia coli were used for the purposes of cloning and conjugation: DH5α , TOP10 F′ (Invitrogen, Carlsbad, CA) and Tam 1 (Active Motif, Carlsbad, CA). .. Most plasmids were constructed using standard molecular biology techniques with restriction enzymes or polymerase chain reaction.

Ligation:

Article Title: Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap
Article Snippet: .. The separated fragment was ligated into the p-Tet-On vector by using T4 DNA Ligase (invitrogen, USA), Ligation reaction was transformed into the Top10 f (Invitrogen, USA) by heat shock method and the accuracy of clones was confirmed by double digestion. ..

Construct:

Article Title: An Allosteric Interaction Network Promotes Conformation State-Dependent Eviction of the Nas6 Assembly Chaperone from Nascent 26S Proteasomes
Article Snippet: .. Plasmids All plasmids were constructed using standard molecular cloning techniques using TOP10 F’ (Invitrogen) as a host strain. .. QuikChange (Agilent) was used for site-directed mutagenesis and the resultant plasmids were sequenced prior to use.

Purification:

Article Title: Non-pathogenic pemphigus foliaceus (PF) IgG acts synergistically with a directly pathogenic PF IgG to increase blistering by p38MAPK-dependent desmoglein 1 clustering
Article Snippet: .. Soluble scFv mAbs were produced in the Top10 F’ strain of E. coli (Invitrogen, Carlsbad, CA) and purified by Talon metal affinity resin (Clontech, Mountain View, CA) as previously described [ ]. .. Divalent IgG1 mAbs were generated from anti-Dsg1 scFv mAbs as described previously [ ].

Produced:

Article Title: Non-pathogenic pemphigus foliaceus (PF) IgG acts synergistically with a directly pathogenic PF IgG to increase blistering by p38MAPK-dependent desmoglein 1 clustering
Article Snippet: .. Soluble scFv mAbs were produced in the Top10 F’ strain of E. coli (Invitrogen, Carlsbad, CA) and purified by Talon metal affinity resin (Clontech, Mountain View, CA) as previously described [ ]. .. Divalent IgG1 mAbs were generated from anti-Dsg1 scFv mAbs as described previously [ ].

Molecular Cloning:

Article Title: An Allosteric Interaction Network Promotes Conformation State-Dependent Eviction of the Nas6 Assembly Chaperone from Nascent 26S Proteasomes
Article Snippet: .. Plasmids All plasmids were constructed using standard molecular cloning techniques using TOP10 F’ (Invitrogen) as a host strain. .. QuikChange (Agilent) was used for site-directed mutagenesis and the resultant plasmids were sequenced prior to use.

other:

Article Title: Improved microtitre plate production of single chain Fv fragments in Escherichia coli.
Article Snippet: The new era of functional genomics demands several antibodies as specific detection reagents for proteins, their complexes and post-translational modifications.

Transformation Assay:

Article Title: Protein evolution with an expanded genetic code
Article Snippet: .. Each of these pCDF plasmids were then transformed into Top10 F' (Invitrogen) to create Keto-X- E. coli , Bpy-X- E. coli , SY-X- E. coli , and Boro-X- E. coli and maintained on the antibiotics chloramphenicol (30 μg/ml) and tetracycline (30 μg/ml). .. A Fab-format antibody containing VH 3–23 and VL A27 with a TAG in the VH CDR3 loop was synthesized by Blue Heron.

Article Title: Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap
Article Snippet: .. The separated fragment was ligated into the p-Tet-On vector by using T4 DNA Ligase (invitrogen, USA), Ligation reaction was transformed into the Top10 f (Invitrogen, USA) by heat shock method and the accuracy of clones was confirmed by double digestion. ..

Plasmid Preparation:

Article Title: Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap
Article Snippet: .. The separated fragment was ligated into the p-Tet-On vector by using T4 DNA Ligase (invitrogen, USA), Ligation reaction was transformed into the Top10 f (Invitrogen, USA) by heat shock method and the accuracy of clones was confirmed by double digestion. ..

Article Title: Discovery of Calcium as a Biofilm-Promoting Signal for Vibrio fischeri Reveals New Phenotypes and Underlying Regulatory Complexity
Article Snippet: .. A variety of E. coli host strains, including GT115 (Invivogen, San Diego, CA), CC118 λ pir , TAM1 or TAM1 λ pir (Active Motif, Carlsbad, CA), DH5α , DH5α λ pir , Top10 F′ (Invitrogen, now Thermo Fisher Scientific), S17-1 λ pir , and π3813 , were used for the purposes of cloning, plasmid maintenance, and conjugation. ..

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    Thermo Fisher competent e coli one shot top10 cells
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    Thermo Fisher top10 f component e
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