top10 electrocomp kit  (Thermo Fisher)


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    Name:
    TOP10 Electrocomp Kit
    Description:
    TOP10 E coli are provided at a transformation efficiency of 1 × 1010 cfu µg supercoiled DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids The genotype of TOP10 cells is similar to the DH10B strain and offers the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non specific digestion by Endonuclease I• recA1 for reduced occurrence of non specific recombination in cloned DNAOne Shot TOP10 Kits are available with either chemically competent or electrocompetent E coli to fit your specific transformation needs TOP10 E coli are also available in the high throughput MultiShot format Note This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns Please see the product manual for reaction scale recommendations Genotype F mcrA Δ mrr hsdRMS mcrBC φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL endA1 nupG
    Catalog Number:
    c66411
    Price:
    None
    Applications:
    Cloning|Electrocompetent Cells for Cloning|Transformation
    Category:
    Competent Cells Strains
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    Structured Review

    Thermo Fisher top10 electrocomp kit
    TOP10 E coli are provided at a transformation efficiency of 1 × 1010 cfu µg supercoiled DNA and are ideal for high efficiency cloning and plasmid propagation They allow stable replication of high copy number plasmids The genotype of TOP10 cells is similar to the DH10B strain and offers the following features • hsdR for efficient transformation of unmethylated DNA from PCR amplifications• mcrA for efficient transformation of methylated DNA from genomic preparations• lacZΔM15 for blue white color screening of recombinant clones• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non specific digestion by Endonuclease I• recA1 for reduced occurrence of non specific recombination in cloned DNAOne Shot TOP10 Kits are available with either chemically competent or electrocompetent E coli to fit your specific transformation needs TOP10 E coli are also available in the high throughput MultiShot format Note This kit contains enough electrocompetent cells for 25 x 40 µL rxns or 50 x 20 µL rxns Please see the product manual for reaction scale recommendations Genotype F mcrA Δ mrr hsdRMS mcrBC φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ ara leu 7697 galU galK rpsL endA1 nupG
    https://www.bioz.com/result/top10 electrocomp kit/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    top10 electrocomp kit - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion
    Article Snippet: .. All cloning was conducted using TOP10 E. coli (Invitrogen, #C664-11). .. Three medias were used: LB Miller (Difco, #244510), L Broth (Difco, #241210) and SPI-1 Inducing Media (IM) (LB, 0.3 M NaCl [ ]).

    Transformation Assay:

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. The product resulting from this ligation, containing the bla CTX-M-15 SNP library named pZS3-CTX-M-15-Mut2Vol2, was transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455) and selected on kanamycin (50 µg/ml). .. The second library was constructed similarly to the first library, except that 8 instead of 20 cycles were run on the error-prone PCR product with primers containing restriction enzyme tails.

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. Each of the resulting plasmids pZS3-CTX-M-15N135D , pZS3-CTX-M-15S133G and pZS3-CTX-M-15G239S were transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455). .. Mutations were verified with Sanger sequencing.

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. Each of the resulting plasmids pZS3-CTX-M-15N135D , pZS3-CTX-M-15S133G and pZS3-CTX-M-15G239S were transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455). .. Mutations were verified with Sanger sequencing.

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. The product resulting from this ligation, containing the bla CTX-M-15 SNP library named pZS3-CTX-M-15-Mut2Vol2, was transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455) and selected on kanamycin (50 µg/ml). .. The second library was constructed similarly to the first library, except that 8 instead of 20 cycles were run on the error-prone PCR product with primers containing restriction enzyme tails.

    Ligation:

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. The product resulting from this ligation, containing the bla CTX-M-15 SNP library named pZS3-CTX-M-15-Mut2Vol2, was transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455) and selected on kanamycin (50 µg/ml). .. The second library was constructed similarly to the first library, except that 8 instead of 20 cycles were run on the error-prone PCR product with primers containing restriction enzyme tails.

    Article Title: Collateral sensitivity constrains resistance evolution of the CTX-M-15 β-lactamase
    Article Snippet: .. The product resulting from this ligation, containing the bla CTX-M-15 SNP library named pZS3-CTX-M-15-Mut2Vol2, was transformed into electrocompetent E. coli TOP10 (ThermoFisher #C66455) and selected on kanamycin (50 µg/ml). .. The second library was constructed similarly to the first library, except that 8 instead of 20 cycles were run on the error-prone PCR product with primers containing restriction enzyme tails.

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  • 94
    Thermo Fisher top10 electrocomp kit
    Top10 Electrocomp Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 electrocomp kit/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    top10 electrocomp kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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