top10 dh5α competent cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher top10 dh5α competent cells
    Top10 Dh5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 dh5α competent cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    top10 dh5α competent cells - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: .. Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.

    Amplification:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.

    Purification:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: .. Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.

    Polymerase Chain Reaction:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: .. Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.

    Sequencing:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: Paragraph title: (iv) Cloning and sequencing. ... Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells.

    Transformation Assay:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: .. Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.

    Plasmid Preparation:

    Article Title: New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿New Genera of RNA Viruses in Subtropical Seawater, Inferred from Polymerase Gene Sequences ▿ †
    Article Snippet: .. Purified PCR products were cloned directly into the TOPO-TA vector (Invitrogen) and transformed into TOP10 DH5α competent cells (Invitrogen) according to instructions provided with the vector and cells. .. Clones were screened for inserts by PCR amplification with the universal M13 forward (−20; 5′-GTAAAACGACGGCCAGT-3′) and M13 reverse (−27; 5′-CAGGAAACAGCTATGAC-3′) primers.