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Thermo Fisher tol2kit
Tol2kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tol2kit/product/Thermo Fisher
Average 88 stars, based on 13 article reviews
Price from $9.99 to $1999.99
tol2kit - by Bioz Stars, 2020-09
88/100 stars

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Clone Assay:

Article Title: Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression
Article Snippet: .. Generation of the Tg(hsp70 :hmx3a) line The zebrafish hmx3a cDNA sequence (RefSeq NM_131634.2), including the complete open reading frame, endogenous Kozak sequence and 15 bp of 3’ UTR, was cloned into a Tol2 -containing ubi :tdTomato destination vector, flanked by a 5’ hsp70 promoter and a 3’ SV40 late polyadenylation signal sequence, using the Tol2kit [ ] (Invitrogen). .. 50 ng of this construct were injected into one-cell stage embryos together with 50 ng of in vitro-transcribed transposase RNA.

Article Title: A familial congenital heart disease with a possible multigenic origin involving a mutation in BMPR1A
Article Snippet: .. Cloning Plasmids were generated using the Tol2kit and the Gateway system by Invitrogen . .. Expression clones used for RNA transcription contain a CMV/SP6 promoter.

Article Title: Asymmetric inhibition of Ulk2 causes left-right differences in habenular neuropil formation
Article Snippet: .. All transgenic animals generated for this study were created using the Tol2kit ( ) and built using the Multisite Gateway (Invitrogen) cloning system in the pDestTol2CG2 transgenesis vector. .. For stable germline transgenics (Tg[UAS: kctd12.1:mt ]vu260/264 , Tg[UAS: kctd12.1:pA ]vu302 , Tg[UAS: kctd12.2:mt ]vu431 ), AB embryos were injected at late one-cell stage, screened for cardiac GFP at 3dpf, raised to adulthood, and outcrossed to AB animals.

Sequencing:

Article Title: Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression
Article Snippet: .. Generation of the Tg(hsp70 :hmx3a) line The zebrafish hmx3a cDNA sequence (RefSeq NM_131634.2), including the complete open reading frame, endogenous Kozak sequence and 15 bp of 3’ UTR, was cloned into a Tol2 -containing ubi :tdTomato destination vector, flanked by a 5’ hsp70 promoter and a 3’ SV40 late polyadenylation signal sequence, using the Tol2kit [ ] (Invitrogen). .. 50 ng of this construct were injected into one-cell stage embryos together with 50 ng of in vitro-transcribed transposase RNA.

In Vitro:

Article Title: Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish
Article Snippet: .. The pCS2FA-transposase clone (Tol2kit; #396) was digested with PmeI , and Tol2 transposase mRNA was transcribed, capped and polyadenylated in vitro using the mMessage SP6 kit (Ambion; AM1340) and the Poly(A) Tailing Kit (Ambion; AM1350). mRNA was purified by precipitation using 2.5 M LiCl. .. Transposase mRNA (37 ng µl−1 ) and each of the Tg(Hemgn:mCherry,myl7:EGFP) expression clones (25 ng µl−1 ) were co-injected into one-cell wild-type zebrafish embryos.

Purification:

Article Title: Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish
Article Snippet: .. The pCS2FA-transposase clone (Tol2kit; #396) was digested with PmeI , and Tol2 transposase mRNA was transcribed, capped and polyadenylated in vitro using the mMessage SP6 kit (Ambion; AM1340) and the Poly(A) Tailing Kit (Ambion; AM1350). mRNA was purified by precipitation using 2.5 M LiCl. .. Transposase mRNA (37 ng µl−1 ) and each of the Tg(Hemgn:mCherry,myl7:EGFP) expression clones (25 ng µl−1 ) were co-injected into one-cell wild-type zebrafish embryos.

Transgenic Assay:

Article Title: Asymmetric inhibition of Ulk2 causes left-right differences in habenular neuropil formation
Article Snippet: .. All transgenic animals generated for this study were created using the Tol2kit ( ) and built using the Multisite Gateway (Invitrogen) cloning system in the pDestTol2CG2 transgenesis vector. .. For stable germline transgenics (Tg[UAS: kctd12.1:mt ]vu260/264 , Tg[UAS: kctd12.1:pA ]vu302 , Tg[UAS: kctd12.2:mt ]vu431 ), AB embryos were injected at late one-cell stage, screened for cardiac GFP at 3dpf, raised to adulthood, and outcrossed to AB animals.

Article Title: Genetic and neuronal regulation of sleep by neuropeptide VF
Article Snippet: .. Tg(UAS:TRPV1-TagRFP-T;cmlc2:EGFP) We generated the Tg(UAS:TRPV1-TagRFP-T;cmlc2:EGFP) transgenic line (ct851Tg, RRID: ZDB-ALT-170927-7 ) using the Tol2kit ( ) and multisite Gateway Technology (Invitrogen). .. The middle entry clone (pME-TRPV1-TagRFP-T) was generated using a BP reaction by combining a donor vector (pDONR221) and TRPV1-TagRFP-T ( ).

Generated:

Article Title: A familial congenital heart disease with a possible multigenic origin involving a mutation in BMPR1A
Article Snippet: .. Cloning Plasmids were generated using the Tol2kit and the Gateway system by Invitrogen . .. Expression clones used for RNA transcription contain a CMV/SP6 promoter.

Article Title: Asymmetric inhibition of Ulk2 causes left-right differences in habenular neuropil formation
Article Snippet: .. All transgenic animals generated for this study were created using the Tol2kit ( ) and built using the Multisite Gateway (Invitrogen) cloning system in the pDestTol2CG2 transgenesis vector. .. For stable germline transgenics (Tg[UAS: kctd12.1:mt ]vu260/264 , Tg[UAS: kctd12.1:pA ]vu302 , Tg[UAS: kctd12.2:mt ]vu431 ), AB embryos were injected at late one-cell stage, screened for cardiac GFP at 3dpf, raised to adulthood, and outcrossed to AB animals.

Article Title: Live imaging of collagen deposition during skin development and repair in a collagen I – GFP fusion transgenic zebrafish line
Article Snippet: .. A destination vector, pDEST-krt19:col1a2-GFP , was generated utilising pME-Zcol1a2GFP, p5E-krtt1c19e ; , p3E-pA, pDESTTol2pA2 from the Tol2kit , in a LR Clonase II Plus enzyme mediated LR Gateway recombination reaction (Invitrogen). .. 3.3 Generation of collagen I α2 – GFP/mCherry expressing zebrafish A 1 nl volume of expression vector pDEST-krt19:col1a2GFP construct at 62.5 ng μl−1 , together with 50 ng μl−1 purified Tol2 mRNA was microinjected into Casper zebrafish one cell stage embryos, as previously described ( ).

Article Title: Genetic and neuronal regulation of sleep by neuropeptide VF
Article Snippet: .. Tg(UAS:TRPV1-TagRFP-T;cmlc2:EGFP) We generated the Tg(UAS:TRPV1-TagRFP-T;cmlc2:EGFP) transgenic line (ct851Tg, RRID: ZDB-ALT-170927-7 ) using the Tol2kit ( ) and multisite Gateway Technology (Invitrogen). .. The middle entry clone (pME-TRPV1-TagRFP-T) was generated using a BP reaction by combining a donor vector (pDONR221) and TRPV1-TagRFP-T ( ).

Polymerase Chain Reaction:

Article Title: Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction
Article Snippet: .. The PCR product was recombined with the donor vector pDONR221 from the Tol2Kit via BP clonase (Invitrogen). .. This plasmid was then recombined with p5E-hsp70l, p3E-EGFPpA, and pDestTol2pA2 from the Tol2Kit, via LR clonase (Invitrogen) to create the final Tg(HSP:mmp11-EGFP) construct.

Plasmid Preparation:

Article Title: Anteroposterior patterning of the zebrafish ear through Fgf- and Hh-dependent regulation of hmx3a expression
Article Snippet: .. Generation of the Tg(hsp70 :hmx3a) line The zebrafish hmx3a cDNA sequence (RefSeq NM_131634.2), including the complete open reading frame, endogenous Kozak sequence and 15 bp of 3’ UTR, was cloned into a Tol2 -containing ubi :tdTomato destination vector, flanked by a 5’ hsp70 promoter and a 3’ SV40 late polyadenylation signal sequence, using the Tol2kit [ ] (Invitrogen). .. 50 ng of this construct were injected into one-cell stage embryos together with 50 ng of in vitro-transcribed transposase RNA.

Article Title: Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction
Article Snippet: .. The PCR product was recombined with the donor vector pDONR221 from the Tol2Kit via BP clonase (Invitrogen). .. This plasmid was then recombined with p5E-hsp70l, p3E-EGFPpA, and pDestTol2pA2 from the Tol2Kit, via LR clonase (Invitrogen) to create the final Tg(HSP:mmp11-EGFP) construct.

Article Title: Asymmetric inhibition of Ulk2 causes left-right differences in habenular neuropil formation
Article Snippet: .. All transgenic animals generated for this study were created using the Tol2kit ( ) and built using the Multisite Gateway (Invitrogen) cloning system in the pDestTol2CG2 transgenesis vector. .. For stable germline transgenics (Tg[UAS: kctd12.1:mt ]vu260/264 , Tg[UAS: kctd12.1:pA ]vu302 , Tg[UAS: kctd12.2:mt ]vu431 ), AB embryos were injected at late one-cell stage, screened for cardiac GFP at 3dpf, raised to adulthood, and outcrossed to AB animals.

Article Title: Live imaging of collagen deposition during skin development and repair in a collagen I – GFP fusion transgenic zebrafish line
Article Snippet: .. A destination vector, pDEST-krt19:col1a2-GFP , was generated utilising pME-Zcol1a2GFP, p5E-krtt1c19e ; , p3E-pA, pDESTTol2pA2 from the Tol2kit , in a LR Clonase II Plus enzyme mediated LR Gateway recombination reaction (Invitrogen). .. 3.3 Generation of collagen I α2 – GFP/mCherry expressing zebrafish A 1 nl volume of expression vector pDEST-krt19:col1a2GFP construct at 62.5 ng μl−1 , together with 50 ng μl−1 purified Tol2 mRNA was microinjected into Casper zebrafish one cell stage embryos, as previously described ( ).

Article Title: Disruption of Trim9 function abrogates macrophage motility in vivo
Article Snippet: .. The pDestTol2pA2 vector and pCS2FA‐transposase were obtained in the Tol2kit [ ] and from Koichi Kawakami. pDestTol2pA2 was restriction digested with Xho I and Cla I (Thermo Fisher Scientific). .. Amplicons were cloned into the linearized pDestTol2pA2 vector using Gibson Assembly Master Mix (New England BioLabs, Ipswich, MA, USA) according to manufacturer instructions.

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    Thermo Fisher p5 uas vector
    P5 Uas Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5 uas vector/product/Thermo Fisher
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    Thermo Fisher tol2kit
    Tol2kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tol2kit/product/Thermo Fisher
    Average 88 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    tol2kit - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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