tofacitinib mce  (MedChemExpress)


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    Name:
    Tofacitinib
    Description:
    Tofacitinib is an orally available JAK3 2 1 inhibitor with IC50s of 1 20 and 112 nM respectively
    Catalog Number:
    HY-40354
    Price:
    132.0
    Category:
    Reference Compound
    Applications:
    COVID-19-immunoregulation
    Purity:
    98.14%
    Size:
    100mg
    Formula:
    C16H20N6O
    Molecular Weight:
    312.37
    Molecule Name:
    Tofacitinib
    Buy from Supplier


    Structured Review

    MedChemExpress tofacitinib mce
    Tofacitinib is an orally available JAK3 2 1 inhibitor with IC50s of 1 20 and 112 nM respectively
    https://www.bioz.com/result/tofacitinib mce/product/MedChemExpress
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tofacitinib mce - by Bioz Stars, 2021-07
    96/100 stars

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    other:

    Article Title: Paeoniflorin-6'-o-benzene sulfonate (CP-25) improves vasculitis through inhibiting IL-17A/JAK/STAT3 signaling pathway in endothelial cells of HFD CIA rats.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease that affects not only joints but also multiple organ systems including cardiovascular system.. Endothelial dysfunction plays an important role in cardiovascular diseases (CVD).

    Recombinant:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

    SYBR Green Assay:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

    Reporter Assay:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

    Transfection:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

    Polyacrylamide Gel Electrophoresis:

    Article Title: The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis.
    Article Snippet: KEY RESOURCES TABLEREAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-F4/80 antibody [CI:A3-1] Abcam Cat# ab6640; RRID:AB_1140040 Anti-iNOS antibody Abcam Cat# ab15323; RRID:AB_301857 Phospho-IKKa (Ser176)/IKKb (Ser177) Rabbit mAb Cell Signaling Technology Cat# 2078; RRID:AB_2079379 Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb Cell Signaling Technology Cat# 3033; RRID:AB_33128 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology Cat# 4370; RRID:AB_2315112 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP Rabbit mAb Cell Signaling Technology Cat# 4511; RRID:AB_2139682 b-actin (8H10D10) Mouse mAb (HRP Conjugate) Cell Signaling Technology Cat# 12262; RRID:AB_2566811 IkBa Antibody Cell Signaling Technology Cat# 9242; RRID:AB_331623 Phospho-Jak1 (Tyr1034/1035) Antibody Cell Signaling Technology Cat# 3331; RRID:AB_2265057 Phospho-Jak2 (Tyr1007/1008) Antibody Cell Signaling Technology Cat# 3771; RRID:AB_330403 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat# 9145; RRID:AB_2491009 Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb Cell Signaling Technology Cat# 4322; RRID:AB_10544692 HRP-GAPDH Proteintech Cat# 60004-1-Ig; RRID:AB_2107436 Rabbit anti-HA (clone C29F4) Cell Signaling Technology Cat# 3724; RRID:AB_1549585 Flag-Tag Rabbit mAb Cell Signaling Technology Cat# 14793; RRID:AB_2572291 a-Tubulin (11H10) Rabbit mAb Cell Signaling Technology Cat# 2125; RRID:AB_2619646 Jak2 (D2E12) XP Rabbit mAb Cell Signaling Technology Cat# 3230; RRID:AB_2128522 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID:AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat anti-rabbit IgG H (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 Goat Anti-Mouse IgG H (Cy3 ) preadsorbed Abcam Cat# ab97035; RRID:AB_10680176 Anti-Biotin antibody Abcam Cat# ab53494; RRID:AB_867860 Goat-anti-Rat IgG (Alexa Fluor 647) Abcam Cat# ab150167 Goat-Anti-Rabbit IgG (Alexa Fluor 488) Abcam Cat# ab150077; RRID:AB_2630356 PE-Stat6-pY641 BD Biosciences Cat# 612701; RRID:AB_399940 APC-Stat3-pY705 BD Biosciences Cat# 557815; RRID:AB_647144 PE-Stat5-pY694 BD Biosciences Cat# 612567; RRID:AB_399858 FITC-Stat1-pY701 BD Biosciences Cat# 612596; RRID:AB_399879 Annexin V eBioscience Cat# BMS306APC-20 Bacterial and Virus Strains Trans5a Chemically Competent Cell TransGen Biotech Cat# CD201 Transetta(DE3) Chemically Competent Cell TransGen Biotech Cat# CD801 Biological Samples Healthy adult Peripheral blood Human studies were approved by LongHua Hospital, Shanghai University of Traditional Chinese Medicine (approval number 2018LCSY059) N/A (Continued on next page) Cell Reports 32, 108158, September 15, 2020 e1. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Notopterol ChemFaces Biochemical Cat# CFN98563, CAS: 88206-46-6 Tofacitinib MCE Cat# HY-40354 Ruxolitinib MCE Cat# HY-50856 SD1029 Sigma Cat# 573098 SH-4-54 MCE Cat# HY-16975 DAPI (4’,6-diamidino-2-phenylindole Sigma-Aldrich Cat# D9564 Lipofectamine RNAiMAX Thermo Fisher Cat# 13778030 Lipofectamine 2000 Life Technologies Cat# 11668019 SYBR Green Master Mix Yeasen biotech Cat# 11201ES03 M-MLV transcriptase Takara Cat# 2640A Recombinant Human IFN-b PeproTech Cat# 300-02BC Recombinant Human IFN-g PeproTech Cat# 300-02 Recombinant Human IL-6 PeproTech Cat# 200-06 Recombinant Human G-CSF Novoprotein Cat# C002 Recombinant Human GM-CSF Novoprotein Cat# C003 Recombinant Human IL-2 PeproTech Cat# 200-02 Recombinant Human IL-4 PeproTech Cat# 200-04 Recombinant Human IL-13 PeproTech Cat# 200-13 Recombinant Human Growth hormone/GH Novoprotein Cat# C725 Recombinant Human Prolactin/PRL Novoprotein Cat# C736 Recombinant Murine Thrombopoietin PeproTech Cat# 315-14 Recombinant Murine M-CSF PeproTech cat # 315-02 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat# E1910 ELISA kits for murine IL-1b NeoBioscience Cat# EMC001b ELISA kits for murine IL-6 NeoBioscience Cat# EMC004 ELISA kits for murine TNF-a NeoBioscience Cat# EMC102a PrimeScriptTM RT reagent Kit Takara Cat# RR047A Lipofectamine 2000 reagent Invitrogen Cat# 11668-027 polybrene Sigma Cat# TR-1003 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat# 13778075 mMESSAGE mMACHINE T7 Transcription Kit Invitrogen Cat# AM1344 Deposited Data Raw and analyzed data This paper SRA accession: PRJNA656866 Experimental Models: Cell Lines HEK293T cells ATCC TIB-71 iBMDMs Laboratory of Feng Shao N/A RAW264.7 macrophages ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57/BL6 Shanghai Slack Laboratory Animals N/A Mouse: DBA/1J Shanghai Slack Laboratory Animals N/A Zebrafish Zebrafish Technology Platform, SIBCB N/A Oligonucleotides See Table S1 for oligonucleotide information This paper N/A (Continued on next page) e2 Cell Reports 32, 108158, September 15, 2020. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA STAT5 luciferase reporter plasmids (pGMSTAT5-Lu) Shanghai Yeasen Biological Technology Co., Ltd. Cat# 11554ES03 pRL-SV40-C Beyotime Biotechnology Cat# D2768-1 mg pMIGR1-3 3 HA-JAK1 This paper N/A pMIGR1-3 3 HA-JAK2 This paper N/A pMIGR1-3 3 HA-JAK3 This paper N/A pMIGR1-3 3 HA-TYK2 This paper N/A pMIGR1-3 3 HA-JH1JH2 This paper N/A pMIGR1-3 3 HA-JH1JH2-3A This paper N/A pMIGR1-3 3 HA-JH1-3A This paper N/A pMIGR1-3 3 HA-JH1-932A This paper N/A pMIGR1-3 3 HA-JH1-980A This paper N/A pMIGR1-3 3 HA-JH1-981A This paper N/A pMIGR1-3 3 HA-JH1-932/980A This paper N/A pMIGR1-3 3 HA-JH1-932/981A This paper N/A pMIGR1-3 3 HA-JH1-980/981A This paper N/A pET-28a-His-JH1 This paper N/A pET-28a-His-JH1-3A This paper N/A MIGR-HA-JAK1 This paper N/A pCL-10A1 Retrovirus Packaging Vector Novus Biologicals NBP2-29542 Software and Algorithms micro-CT Evaluation program V6.6 Scanco Medical AG, Switzerland N/A GraphPad Prism version 6.0 GraphPad Software https://www.graphpad.com/ FlowJo v10 FlowJo https://www.flowjo.com/solutions/flowjo Surflex-Dock version 2.6 Surflex-Dock https://www.scientific-computing.com/ press-releases/sybyl-x-21 SYBYL 2.0 N/A https://sybyl-x.software.informer.com/2.0/ Image-Pro 6.0 NIH https://imagej.nih.gov/ij CFX ManagerTM version 2.1 Bio-Rad N/A Other Sequence data, analyses This paper https://www.ncbi.nlm.nih.gov/sra/ PRJNA656866 chicken type II collagen Chondrex Cat# 20011 complete Freund’s adjuvant Chondrex Cat# 7001 incomplete Freund’s adjuvant Chondrex Cat# 7002 bovine type II collagen Chondrex Cat# 20021 BeyoGold His-tag Purification Resin Beyotime Biotechnology cat # P2210 AKATA anion exchange column Thermo Scientific cat # 088765 Phosflow Fix Buffer I BD PharMingen Cat# 557870 Phosflow Perm Buffer III BD PharMingen Cat# 558050.

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