tnf alpha cdna orf clone mouse c dykddddk tag  (Sino Biological)


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    Name:
    TNF alpha cDNA ORF Clone Mouse C DYKDDDDK tag
    Description:
    Full length Clone DNA of Mouse tumor necrosis factor with C terminal Flag tag
    Catalog Number:
    MG50349-CF
    Price:
    195.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    DIF cDNA ORF Clone Mouse, TNF-a cDNA ORF Clone Mouse, TNF-alpha cDNA ORF Clone Mouse, Tnfa cDNA ORF Clone Mouse, TNFalpha cDNA ORF Clone Mouse, Tnfsf1a cDNA ORF Clone Mouse, TNFSF2 cDNA ORF Clone Mouse
    Molecule Name:
    TNF,TNFSF2,TNF-alpha,
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    Structured Review

    Sino Biological tnf alpha cdna orf clone mouse c dykddddk tag
    TNF alpha cDNA ORF Clone Mouse C DYKDDDDK tag
    Full length Clone DNA of Mouse tumor necrosis factor with C terminal Flag tag
    https://www.bioz.com/result/tnf alpha cdna orf clone mouse c dykddddk tag/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf alpha cdna orf clone mouse c dykddddk tag - by Bioz Stars, 2021-05
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ
    Article Snippet: The murine TNF-α-coding gene (Tnfa ) (GenBank No. NM_013693.2) fused to a Flag-tag sequence was inserted into the pSFV1-NruI vector under the control of the SFV 26S subgenomic promoter. .. The Tnfa-Flag fragment was amplified from the template plasmid pCMV3-mTNF-Flag (Cat. No. MG50349-CF; Sino Biological Inc., Beijing, China) using PCR with the following primers (synthesized by Microsynth AG, Balgach, Switzerland): 5′-GC GGATCC ATGAGCACAGAAAGCATGATC-3′ (forward) and 5′-TG CCCGGG TTTACTTATCGTCGTCATCCTTG-3′ (reverse). .. BamH I and SmaI restriction sites (shown here in bold) were introduced to facilitate the ligation of the PCR fragment into the pSFV1-NruI vector.

    Plasmid Preparation:

    Article Title: Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ
    Article Snippet: The murine TNF-α-coding gene (Tnfa ) (GenBank No. NM_013693.2) fused to a Flag-tag sequence was inserted into the pSFV1-NruI vector under the control of the SFV 26S subgenomic promoter. .. The Tnfa-Flag fragment was amplified from the template plasmid pCMV3-mTNF-Flag (Cat. No. MG50349-CF; Sino Biological Inc., Beijing, China) using PCR with the following primers (synthesized by Microsynth AG, Balgach, Switzerland): 5′-GC GGATCC ATGAGCACAGAAAGCATGATC-3′ (forward) and 5′-TG CCCGGG TTTACTTATCGTCGTCATCCTTG-3′ (reverse). .. BamH I and SmaI restriction sites (shown here in bold) were introduced to facilitate the ligation of the PCR fragment into the pSFV1-NruI vector.

    Polymerase Chain Reaction:

    Article Title: Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ
    Article Snippet: The murine TNF-α-coding gene (Tnfa ) (GenBank No. NM_013693.2) fused to a Flag-tag sequence was inserted into the pSFV1-NruI vector under the control of the SFV 26S subgenomic promoter. .. The Tnfa-Flag fragment was amplified from the template plasmid pCMV3-mTNF-Flag (Cat. No. MG50349-CF; Sino Biological Inc., Beijing, China) using PCR with the following primers (synthesized by Microsynth AG, Balgach, Switzerland): 5′-GC GGATCC ATGAGCACAGAAAGCATGATC-3′ (forward) and 5′-TG CCCGGG TTTACTTATCGTCGTCATCCTTG-3′ (reverse). .. BamH I and SmaI restriction sites (shown here in bold) were introduced to facilitate the ligation of the PCR fragment into the pSFV1-NruI vector.

    Synthesized:

    Article Title: Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ
    Article Snippet: The murine TNF-α-coding gene (Tnfa ) (GenBank No. NM_013693.2) fused to a Flag-tag sequence was inserted into the pSFV1-NruI vector under the control of the SFV 26S subgenomic promoter. .. The Tnfa-Flag fragment was amplified from the template plasmid pCMV3-mTNF-Flag (Cat. No. MG50349-CF; Sino Biological Inc., Beijing, China) using PCR with the following primers (synthesized by Microsynth AG, Balgach, Switzerland): 5′-GC GGATCC ATGAGCACAGAAAGCATGATC-3′ (forward) and 5′-TG CCCGGG TTTACTTATCGTCGTCATCCTTG-3′ (reverse). .. BamH I and SmaI restriction sites (shown here in bold) were introduced to facilitate the ligation of the PCR fragment into the pSFV1-NruI vector.

    Construct:

    Article Title: Generation and Functional In Vitro Analysis of Semliki Forest Virus Vectors Encoding TNF-α and IFN-γ
    Article Snippet: Xma I and Apa I (shown here in bold) restriction sites were incorporated to facilitate cloning into the pSFV1/Enh-Luc vector. .. The sequences Tnfa-Flag and Enh-2A-Ifng of the constructs were verified by GATC Biotech AG (Constance, Germany) and found to share 100% identity with the reference sequences Tnfa (GenBank No. NM_013693.2), Flag (shown in the product description of pCMV3-mTNF-Flag (Cat. No. MG50349-CF; Sino Biological)), Enh-2A [shown in Ref. ( )] and Ifng (NM_008337.3). .. RNA Synthesis and Production of Viral Particles RNAs were synthesized and transfected into BHK-21 cells using electroporation as previously described ( ).

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  • 90
    Sino Biological tnf alpha cdna orf clone mouse c dykddddk tag
    Tnf Alpha Cdna Orf Clone Mouse C Dykddddk Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf alpha cdna orf clone mouse c dykddddk tag/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf alpha cdna orf clone mouse c dykddddk tag - by Bioz Stars, 2021-05
    90/100 stars
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