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(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with <t>TLQP-21</t> treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.
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(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with <t>TLQP-21</t> treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.
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(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with <t>TLQP-21</t> treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.
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(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with <t>TLQP-21</t> treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.
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murine purified tlqp-21  (Millipore)
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(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with <t>TLQP-21</t> treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.
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https://www.bioz.com/result/murine purified tlqp-21/product/Millipore
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murine purified tlqp-21 - by Bioz Stars, 2025-12
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Image Search Results


(A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with TLQP-21 treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.

Journal: PLoS ONE

Article Title: TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression

doi: 10.1371/journal.pone.0079760

Figure Lengend Snippet: (A) Annexin-V-PI staining to assess the apoptotic ratios of HUVECs with TLQP-21 treated alone or under high glucose (HG) concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM). (B) Cell viability of HUVECs under HG concentration (30 mmol/L) incubation for 72 h with or without TLQP-21(10–100 nM) protection.* p<.05,** p<.01 vs. saline treated group. (C) Cell viability of HUVECs with etoposide (0.2 mM) treatment for 72 h with or without TLQP-21(10–100 nM) protection. * p<.05, ** p<.01 vs. saline treated group. All data were collected from three independent experiments and are presented as the mean ±SD.

Article Snippet: Human-derived TLQP-21 (TLGPPSALAAAHTHHALPPSA) was obtained from Phoenix pharmaceuticals (USA), and the scrambled peptide Scr-21 (LRPSHTRPAHQSFARPLHRPA) was synthesized by Apeptide (Shanghai, China)

Techniques: Staining, Concentration Assay, Incubation

(A) The absorbance value of MTS assay with different treatments; the mannitol group was set as hyperosmosis control. *p<.05, **p<.01 vs. NC group; # p<.05, ## p<.01 vs. HG group. (B) Cell proliferation curve of HUVECs with different treatments. # p<.05, ## p<.01 NC&TLQP-21 group vs. HG group; *p<.05, **p<.01 HG+TLQP-21 group vs. HG group. (C) NADPH level of HUVECs with different treatments. **p<.01 vs. NC group; ## p<.01 vs. TLQP-21(10 nM) group. (D) Comparison of the NADPH level of HUVECs with and without TLQP-21(50 nM) treatment; the mannitol group was set as a hyperosmosis control. *p<.05, *p<.01 vs. TLQP-21 withdraw group. All data were collected from three independent experiments and are presented as the mean ±SD.

Journal: PLoS ONE

Article Title: TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression

doi: 10.1371/journal.pone.0079760

Figure Lengend Snippet: (A) The absorbance value of MTS assay with different treatments; the mannitol group was set as hyperosmosis control. *p<.05, **p<.01 vs. NC group; # p<.05, ## p<.01 vs. HG group. (B) Cell proliferation curve of HUVECs with different treatments. # p<.05, ## p<.01 NC&TLQP-21 group vs. HG group; *p<.05, **p<.01 HG+TLQP-21 group vs. HG group. (C) NADPH level of HUVECs with different treatments. **p<.01 vs. NC group; ## p<.01 vs. TLQP-21(10 nM) group. (D) Comparison of the NADPH level of HUVECs with and without TLQP-21(50 nM) treatment; the mannitol group was set as a hyperosmosis control. *p<.05, *p<.01 vs. TLQP-21 withdraw group. All data were collected from three independent experiments and are presented as the mean ±SD.

Article Snippet: Human-derived TLQP-21 (TLGPPSALAAAHTHHALPPSA) was obtained from Phoenix pharmaceuticals (USA), and the scrambled peptide Scr-21 (LRPSHTRPAHQSFARPLHRPA) was synthesized by Apeptide (Shanghai, China)

Techniques: MTS Assay

(A) G6PD activity of HUVECs with different treatments. **p<.01 vs. NC group. (B) mRNA level of G6PD with different treatments. **p<.01 vs. NC group (C) Western-blot analysis shows that G6PD expression decreased with G6PD siRNA. (D) G6PD activity decreases with G6PD siRNA. **p<.01 vs. scramble siRNA group. (E) G6PD siRNA attenuates the upregulation of NADPH when treated with TLQP-21. **p<.01 vs. scramble siRNA group. All data were collected from three independent experiments and are presented as the mean ±SD.

Journal: PLoS ONE

Article Title: TLQP-21 Protects Human Umbilical Vein Endothelial Cells against High-Glucose-Induced Apoptosis by Increasing G6PD Expression

doi: 10.1371/journal.pone.0079760

Figure Lengend Snippet: (A) G6PD activity of HUVECs with different treatments. **p<.01 vs. NC group. (B) mRNA level of G6PD with different treatments. **p<.01 vs. NC group (C) Western-blot analysis shows that G6PD expression decreased with G6PD siRNA. (D) G6PD activity decreases with G6PD siRNA. **p<.01 vs. scramble siRNA group. (E) G6PD siRNA attenuates the upregulation of NADPH when treated with TLQP-21. **p<.01 vs. scramble siRNA group. All data were collected from three independent experiments and are presented as the mean ±SD.

Article Snippet: Human-derived TLQP-21 (TLGPPSALAAAHTHHALPPSA) was obtained from Phoenix pharmaceuticals (USA), and the scrambled peptide Scr-21 (LRPSHTRPAHQSFARPLHRPA) was synthesized by Apeptide (Shanghai, China)

Techniques: Activity Assay, Western Blot, Expressing