timp 1  (R&D Systems)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Recombinant Human TIMP 1 Western Blot Standard Protein
    Description:
    The Recombinant Human TIMP 1 Western Blot Standard Protein has been validated for the following applications Western Blot
    Catalog Number:
    wbc021
    Price:
    109
    Applications:
    Western Blot
    Conjugate:
    Unconjugated
    Size:
    1 Vial
    Category:
    Proteins and Enzymes
    Buy from Supplier


    Structured Review

    R&D Systems timp 1
    Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and <t>TIMP-1</t> ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p
    The Recombinant Human TIMP 1 Western Blot Standard Protein has been validated for the following applications Western Blot
    https://www.bioz.com/result/timp 1/product/R&D Systems
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    timp 1 - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice"

    Article Title: Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-28261-9

    Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and TIMP-1 ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p
    Figure Legend Snippet: Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and TIMP-1 ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p

    Techniques Used: Cell Counting, Mouse Assay

    2) Product Images from "Effects of collagen-derived bioactive peptides and natural antioxidant compounds on proliferation and matrix protein synthesis by cultured normal human dermal fibroblasts"

    Article Title: Effects of collagen-derived bioactive peptides and natural antioxidant compounds on proliferation and matrix protein synthesis by cultured normal human dermal fibroblasts

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-28492-w

    The effect of collagen peptides alone and in combination with all other bioactives on MMP-1, MMP-3 and TIMP-1 levels and elastin breakdown in NHDF cultures. MMP-1, MMP-3, TIMP-1 and desmosine were measured in supernatants of NHDF grown in 24 well plates and incubated in media plus collagen peptides alone (C) and in combination with the other constituents listed in Table 1 (All) of ACTIVE ( a,c,e,g ) or FORTE ( b,d,f,h ) for 48 hours. Data was expressed as % of media control, normalised to 100% in each experiment and is presented as mean ± SEM of 3 independent experiments. * /† Indicates P
    Figure Legend Snippet: The effect of collagen peptides alone and in combination with all other bioactives on MMP-1, MMP-3 and TIMP-1 levels and elastin breakdown in NHDF cultures. MMP-1, MMP-3, TIMP-1 and desmosine were measured in supernatants of NHDF grown in 24 well plates and incubated in media plus collagen peptides alone (C) and in combination with the other constituents listed in Table 1 (All) of ACTIVE ( a,c,e,g ) or FORTE ( b,d,f,h ) for 48 hours. Data was expressed as % of media control, normalised to 100% in each experiment and is presented as mean ± SEM of 3 independent experiments. * /† Indicates P

    Techniques Used: Incubation

    3) Product Images from "Altered Cerebrospinal Fluid Proteins in Smith-Lemli-Opitz Syndrome Patients"

    Article Title: Altered Cerebrospinal Fluid Proteins in Smith-Lemli-Opitz Syndrome Patients

    Journal: American journal of medical genetics. Part A

    doi: 10.1002/ajmg.a.37720

    TIMP-1 and TIMP-2 levels in CSF of SLOS patients. Concentrations of (a) TIMP-1 (n=9) and (b) TIMP-2 (n=12) were assessed by ELISA. Both were significantly elevated in SLOS CSF compared to age- and gender-matched controls. (c) TIMP-1 and TIMP-2 levels positively correlated with MMP-2 levels in CSF of SLOS patients. Error bars represent +/− S.D. from the mean.
    Figure Legend Snippet: TIMP-1 and TIMP-2 levels in CSF of SLOS patients. Concentrations of (a) TIMP-1 (n=9) and (b) TIMP-2 (n=12) were assessed by ELISA. Both were significantly elevated in SLOS CSF compared to age- and gender-matched controls. (c) TIMP-1 and TIMP-2 levels positively correlated with MMP-2 levels in CSF of SLOS patients. Error bars represent +/− S.D. from the mean.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    4) Product Images from "Elevated matrix metalloproteinase-9 in patients with systemic sclerosis"

    Article Title: Elevated matrix metalloproteinase-9 in patients with systemic sclerosis

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar1454

    The production of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) from cultured dermal fibroblasts. Dermal fibroblasts were obtained from affected skin of two patients with systemic sclerosis (SSc) and two healthy controls. Second- or third-passage fibroblasts (5 × 10 4 cells) were cultured for 24 hours in Dulbecco's modified Eagle's medium alone and in the presence of IL-1β (10 ng/ml), tumor necrosis factor (TNF)-α (10 ng/ml), transforming growth factor β (TGFβ) (10 ng/ml), IL-1β (10 ng/ml) plus cyclosporin A (CsA) (500 ng/ml), or TNF-α (10 ng/ml) plus CsA (500 ng/ml). The concentrations of MMP-9 in the supernatants were determined by ELISA. Data are expressed as means ± standard error of the mean (SEM) of two independent experiments performed in triplicate using different cell lines. * P
    Figure Legend Snippet: The production of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) from cultured dermal fibroblasts. Dermal fibroblasts were obtained from affected skin of two patients with systemic sclerosis (SSc) and two healthy controls. Second- or third-passage fibroblasts (5 × 10 4 cells) were cultured for 24 hours in Dulbecco's modified Eagle's medium alone and in the presence of IL-1β (10 ng/ml), tumor necrosis factor (TNF)-α (10 ng/ml), transforming growth factor β (TGFβ) (10 ng/ml), IL-1β (10 ng/ml) plus cyclosporin A (CsA) (500 ng/ml), or TNF-α (10 ng/ml) plus CsA (500 ng/ml). The concentrations of MMP-9 in the supernatants were determined by ELISA. Data are expressed as means ± standard error of the mean (SEM) of two independent experiments performed in triplicate using different cell lines. * P

    Techniques Used: Cell Culture, Modification, Enzyme-linked Immunosorbent Assay

    Comparison of serum concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in patients with systemic sclerosis versus healthy controls. Data are presented as means ± standard error of the mean (Mann–Whitney rank sum test).
    Figure Legend Snippet: Comparison of serum concentrations of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in patients with systemic sclerosis versus healthy controls. Data are presented as means ± standard error of the mean (Mann–Whitney rank sum test).

    Techniques Used: MANN-WHITNEY

    5) Product Images from "TLR2 and AP-1/NF-kappaB are involved in the regulation of MMP-9 elicited by heat killed Listeria monocytogenes in human monocytic THP-1 cells"

    Article Title: TLR2 and AP-1/NF-kappaB are involved in the regulation of MMP-9 elicited by heat killed Listeria monocytogenes in human monocytic THP-1 cells

    Journal: Journal of Inflammation (London, England)

    doi: 10.1186/s12950-015-0077-0

    HKLM up-regulates MMP-9 expression in THP-1 cells. THP-1 cells were stimulated with HKLM different concentrations (1-9x10 7 particles/ml). Cells and culture supernatants were collected. Total cellular RNA was isolated and MMP-9 mRNA was quantified by real time PCR. Relative mRNA expression was expressed as fold expression over average of gene expression in vehicle-treated cells. The average gene expression level in vehicle-treated cells was assumed to be 1 (A) . Secreted MMP-9 levels were measured in supernatants by ELISA (B) . THP-1 cells were stimulated with HKLM (3x10 7 /ml), TNF-alphalph (25 ng/ml; positive control) and vehicle (H 2 O; 2ul/ml) for 24 hrs. Cells and culture supernatants were collected. MMP-9 mRNA was quantified by real-time PCR (D) and secreted levels of TIMP-1 and MMP-9 were measured in the supernatants by ELISA (C E) . SEAP reporter activity (degree of NF-κB /AP-1 activation) was determined in supernatants as described in materials and methods (F) . The results obtained from three independent experiments are shown. The data are presented as mean ± SE. An asterisk (*) represents P -value of
    Figure Legend Snippet: HKLM up-regulates MMP-9 expression in THP-1 cells. THP-1 cells were stimulated with HKLM different concentrations (1-9x10 7 particles/ml). Cells and culture supernatants were collected. Total cellular RNA was isolated and MMP-9 mRNA was quantified by real time PCR. Relative mRNA expression was expressed as fold expression over average of gene expression in vehicle-treated cells. The average gene expression level in vehicle-treated cells was assumed to be 1 (A) . Secreted MMP-9 levels were measured in supernatants by ELISA (B) . THP-1 cells were stimulated with HKLM (3x10 7 /ml), TNF-alphalph (25 ng/ml; positive control) and vehicle (H 2 O; 2ul/ml) for 24 hrs. Cells and culture supernatants were collected. MMP-9 mRNA was quantified by real-time PCR (D) and secreted levels of TIMP-1 and MMP-9 were measured in the supernatants by ELISA (C E) . SEAP reporter activity (degree of NF-κB /AP-1 activation) was determined in supernatants as described in materials and methods (F) . The results obtained from three independent experiments are shown. The data are presented as mean ± SE. An asterisk (*) represents P -value of

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Positive Control, Activity Assay, Activation Assay

    6) Product Images from "Matrix metalloproteinases and diabetic foot ulcers: the ratio of MMP-1 to TIMP-1 is a predictor of wound healing"

    Article Title: Matrix metalloproteinases and diabetic foot ulcers: the ratio of MMP-1 to TIMP-1 is a predictor of wound healing

    Journal: Diabetic Medicine

    doi: 10.1111/j.1464-5491.2008.02414.x

    Levels of MMP-1 and TIMP-1 for good and poor healers during the 12-week follow-up period (results are expressed as medians with 25th and 75th percentiles).
    Figure Legend Snippet: Levels of MMP-1 and TIMP-1 for good and poor healers during the 12-week follow-up period (results are expressed as medians with 25th and 75th percentiles).

    Techniques Used:

    The ratio of MMP-1/TIMP-1 is a predictive factor for healing. The ROC analysis gives an area under the curve of 0.821 [confidence interval (CI) 0.6–1.04]. A ratio of 0.39 at week 0 has a sensitivity of 71% and a specificity of 87.5% for detecting a wound area reduction of at least 82% at week 4 (and thus predicting wound healing at week 12).
    Figure Legend Snippet: The ratio of MMP-1/TIMP-1 is a predictive factor for healing. The ROC analysis gives an area under the curve of 0.821 [confidence interval (CI) 0.6–1.04]. A ratio of 0.39 at week 0 has a sensitivity of 71% and a specificity of 87.5% for detecting a wound area reduction of at least 82% at week 4 (and thus predicting wound healing at week 12).

    Techniques Used:

    7) Product Images from "CCR9/CCL25 expression in non-small cell lung cancer correlates with aggressive disease and mediates key steps of metastasis"

    Article Title: CCR9/CCL25 expression in non-small cell lung cancer correlates with aggressive disease and mediates key steps of metastasis

    Journal: Oncotarget

    doi:

    CCL25-induced expression of MMPs and TIMPs in LuCa cells Cells were tested for their capacity to express mRNA and protein for MMP-2 and -9 and TIMP-1 and -2. [A] SCC (NCI-H520) and AC (NCI-H2126) cells were treated for 30 min with 0 or 100 ng/mL of CCL25. Total RNA was isolated, and quantitative real time-PCR analysis was performed for mRNA expression of MMP-2 and -9 and TIMP-1 and -2. Transcript copies were presented relative to copies of 18S rRNA. [B] Active gelatinases (MMP-2 and -9) in culture supernatants were quantified by gelatin zymography. Cells were stimulated with CCL25 (0 or 100 ng/ml) for 24 h. Top: Graph represents densitometric analysis of zymography for control and treated samples, presented as band area, analyzed by ImageJ software. Bottom: Representative zymography. [C] TIMP-1 and TIMP-2 in culture supernatants were quantified by ELISA. Bars represent the concentration (pg/ml) of TIMP-1 and TIMP-2 in culture supernatants collected from cells treated with 0 or 100 ng/ml of CCL25 for 24 h. Asterisks show significant differences between untreated and CCL25-treated LuCa cells. Data was analyzed by non-parametric two tailed t-test and presented as mean +/− S.D., n=2. * p
    Figure Legend Snippet: CCL25-induced expression of MMPs and TIMPs in LuCa cells Cells were tested for their capacity to express mRNA and protein for MMP-2 and -9 and TIMP-1 and -2. [A] SCC (NCI-H520) and AC (NCI-H2126) cells were treated for 30 min with 0 or 100 ng/mL of CCL25. Total RNA was isolated, and quantitative real time-PCR analysis was performed for mRNA expression of MMP-2 and -9 and TIMP-1 and -2. Transcript copies were presented relative to copies of 18S rRNA. [B] Active gelatinases (MMP-2 and -9) in culture supernatants were quantified by gelatin zymography. Cells were stimulated with CCL25 (0 or 100 ng/ml) for 24 h. Top: Graph represents densitometric analysis of zymography for control and treated samples, presented as band area, analyzed by ImageJ software. Bottom: Representative zymography. [C] TIMP-1 and TIMP-2 in culture supernatants were quantified by ELISA. Bars represent the concentration (pg/ml) of TIMP-1 and TIMP-2 in culture supernatants collected from cells treated with 0 or 100 ng/ml of CCL25 for 24 h. Asterisks show significant differences between untreated and CCL25-treated LuCa cells. Data was analyzed by non-parametric two tailed t-test and presented as mean +/− S.D., n=2. * p

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction, Zymography, Software, Enzyme-linked Immunosorbent Assay, Concentration Assay, Two Tailed Test

    8) Product Images from "Ginseng extract and ginsenoside Rb1 attenuate carbon tetrachloride-induced liver fibrosis in rats"

    Article Title: Ginseng extract and ginsenoside Rb1 attenuate carbon tetrachloride-induced liver fibrosis in rats

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/1472-6882-14-415

    Effects of ginsenosides extract and ginsenoside Rb1 on liver fibrosis markers in rats. A : hepatic hydroxyproline level, B : MMP-2 level; C : TIMP-1 level. Data are presented as mean ± SD (n =10). Values not sharing the same letter differ significantly ( p
    Figure Legend Snippet: Effects of ginsenosides extract and ginsenoside Rb1 on liver fibrosis markers in rats. A : hepatic hydroxyproline level, B : MMP-2 level; C : TIMP-1 level. Data are presented as mean ± SD (n =10). Values not sharing the same letter differ significantly ( p

    Techniques Used:

    9) Product Images from "Elevated plasma levels of TIMP-1 in patients with rotator cuff tear"

    Article Title: Elevated plasma levels of TIMP-1 in patients with rotator cuff tear

    Journal: Acta Orthopaedica

    doi: 10.3109/17453674.2012.736174

    Plasma levels (in ng/mL) of TIMP-1 in controls, in patients with partial-thickness tears (PTT), and in patients with full-thickness tears (FTT), as measured by ELISA. â—� Outlier (more than one and a half box lengths away).
    Figure Legend Snippet: Plasma levels (in ng/mL) of TIMP-1 in controls, in patients with partial-thickness tears (PTT), and in patients with full-thickness tears (FTT), as measured by ELISA. â—� Outlier (more than one and a half box lengths away).

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Plasma levels (in ng/mL) of TIMP-1, TIMP-3, and MMP-9 in controls, in patients with partial-thickness tears (PTT), and in patients with full-thickness tears (FTT), as measured by multiplex analysis. â—� Outlier (more than one and a half box lengths away). â—� Extreme outlier (more than three box lengths away).
    Figure Legend Snippet: Plasma levels (in ng/mL) of TIMP-1, TIMP-3, and MMP-9 in controls, in patients with partial-thickness tears (PTT), and in patients with full-thickness tears (FTT), as measured by multiplex analysis. â—� Outlier (more than one and a half box lengths away). â—� Extreme outlier (more than three box lengths away).

    Techniques Used: Multiplex Assay

    10) Product Images from "Tissue inhibitor of metalloproteinase 1 (TIMP-1) deficiency exacerbates carbon tetrachloride-induced liver injury and fibrosis in mice: involvement of hepatocyte STAT3 in TIMP-1 production"

    Article Title: Tissue inhibitor of metalloproteinase 1 (TIMP-1) deficiency exacerbates carbon tetrachloride-induced liver injury and fibrosis in mice: involvement of hepatocyte STAT3 in TIMP-1 production

    Journal: Cell & Bioscience

    doi: 10.1186/2045-3701-1-14

    TIMP-1 is a survival factor for hepatocytes in vitro . Hepatocytes (2 × 10 5 cells/per well) were cultured in 6-well plates and incubated with or without cycloheximide (CHX) (100 μM) in the absence or presence of TIMP-1(100 ng/ml or 200 ng/ml). Hepatocyte death was determined by measurement of AST levels in the supernatants. *P
    Figure Legend Snippet: TIMP-1 is a survival factor for hepatocytes in vitro . Hepatocytes (2 × 10 5 cells/per well) were cultured in 6-well plates and incubated with or without cycloheximide (CHX) (100 μM) in the absence or presence of TIMP-1(100 ng/ml or 200 ng/ml). Hepatocyte death was determined by measurement of AST levels in the supernatants. *P

    Techniques Used: In Vitro, Cell Culture, Incubation, AST Assay

    TIMP-1 production is reduced in STAT3 Hep-/- mice during chronic liver injury . A , Mice were treated with CCl 4 for 4 weeks and euthanized at various time points. Liver tissues were collected and analyzed for TIMP-1, TIMP-2, TIMP-3 and MMP-9. Value from wild-type mice without CCl 4 treatment was set as 1. B , Serum levels of TIMP-1 protein at various time points post 4-week-CCl 4 treatment. * P
    Figure Legend Snippet: TIMP-1 production is reduced in STAT3 Hep-/- mice during chronic liver injury . A , Mice were treated with CCl 4 for 4 weeks and euthanized at various time points. Liver tissues were collected and analyzed for TIMP-1, TIMP-2, TIMP-3 and MMP-9. Value from wild-type mice without CCl 4 treatment was set as 1. B , Serum levels of TIMP-1 protein at various time points post 4-week-CCl 4 treatment. * P

    Techniques Used: Mouse Assay

    TIMP-1 -/- mice are more susceptible to CCl 4 -induced acute liver injury . A, B , Wild-type and TIMP-1 -/- mice were treated with CCl 4 for 12 or 24 hours. Serum ALT levels were assayed (A). The liver tissue sections were stained with H E. Representative pictures are shown (original magnification × 100) (B). C, D , TUNEL positive hepatocytes were counted (C) and representative pictures of TUNEL staining are shown (D). Values represent means ± SEM (n = 6-10) * P
    Figure Legend Snippet: TIMP-1 -/- mice are more susceptible to CCl 4 -induced acute liver injury . A, B , Wild-type and TIMP-1 -/- mice were treated with CCl 4 for 12 or 24 hours. Serum ALT levels were assayed (A). The liver tissue sections were stained with H E. Representative pictures are shown (original magnification × 100) (B). C, D , TUNEL positive hepatocytes were counted (C) and representative pictures of TUNEL staining are shown (D). Values represent means ± SEM (n = 6-10) * P

    Techniques Used: Mouse Assay, Staining, TUNEL Assay

    TIMP-1 -/- mice have greater liver fibrosis than wild-type mice post 4-week-CCl4 treatment . Mice were treated with CCl 4 for 4 weeks and euthanized 24 hours following the last injection. A, B , Liver tissues were collected for Sirius red staining (A) and immunohistochemical staining with α-SMA antibodies (B). The surface areas stained with Sirius red or α-SMA were quantified and shown in the right panel. C , Western blot analyses of α-SMA from liver tissue protein extracts. The values represent values ± SEM (n = 5-8). * P
    Figure Legend Snippet: TIMP-1 -/- mice have greater liver fibrosis than wild-type mice post 4-week-CCl4 treatment . Mice were treated with CCl 4 for 4 weeks and euthanized 24 hours following the last injection. A, B , Liver tissues were collected for Sirius red staining (A) and immunohistochemical staining with α-SMA antibodies (B). The surface areas stained with Sirius red or α-SMA were quantified and shown in the right panel. C , Western blot analyses of α-SMA from liver tissue protein extracts. The values represent values ± SEM (n = 5-8). * P

    Techniques Used: Mouse Assay, Injection, Staining, Immunohistochemistry, Western Blot

    TIMP-1 -/- mice are more susceptible to CCl4-induced chronic liver injury . Wild-type and TIMP-1 -/- mice were treated with CCl 4 for 4 weeks, and euthanized at various time points after the last injection. A , Serum ALT levels were assayed. B , The liver sections were stained with H E and representative pictures are shown (original magnification × 100). C, D , TUNEL positive hepatocytes were counted (C) and representative pictures of TUNEL staining are shown (D). * P
    Figure Legend Snippet: TIMP-1 -/- mice are more susceptible to CCl4-induced chronic liver injury . Wild-type and TIMP-1 -/- mice were treated with CCl 4 for 4 weeks, and euthanized at various time points after the last injection. A , Serum ALT levels were assayed. B , The liver sections were stained with H E and representative pictures are shown (original magnification × 100). C, D , TUNEL positive hepatocytes were counted (C) and representative pictures of TUNEL staining are shown (D). * P

    Techniques Used: Mouse Assay, Injection, Staining, TUNEL Assay

    IL-6 induction of TIMP-1 in primary hepatocytes is mediated via a STAT3-dependent mechanism . A , Hepatocytes (2 × 10 5 cells/per well) from wide-type or STAT3 Hep-/- mice were cultured in 6-well plates and incubated with or without IL-6 (50 ng/ml) for 6 and 24 h, followed by real-time PCR analysis of TIMP-1 mRNA, B , or cultured for 24 and 48 h, followed by collection of the supernatants for measurement of TIMP-1 protein. Values are means ± SE from 4 independent experiments. * P
    Figure Legend Snippet: IL-6 induction of TIMP-1 in primary hepatocytes is mediated via a STAT3-dependent mechanism . A , Hepatocytes (2 × 10 5 cells/per well) from wide-type or STAT3 Hep-/- mice were cultured in 6-well plates and incubated with or without IL-6 (50 ng/ml) for 6 and 24 h, followed by real-time PCR analysis of TIMP-1 mRNA, B , or cultured for 24 and 48 h, followed by collection of the supernatants for measurement of TIMP-1 protein. Values are means ± SE from 4 independent experiments. * P

    Techniques Used: Mouse Assay, Cell Culture, Incubation, Real-time Polymerase Chain Reaction

    Up-regulation of hepatic and serum TIMP-1 levels following CCl 4 -induced acute or chronic liver injury in mice . A , Mice were treated with a single dose of CCl 4 injection or vehicle (olive oil) injection. Liver tissues were then collected and subject to real-time PCR analysis of hepatic TIMP-1 mRNA (top panel). Sera were collected for measurement of TIMP-1 protein by ELISA (low panel). B , Mice were treated chronically with CCl 4 or vehicle (olive oil) for 4 weeks. Liver tissues and sera were collected 24 and 48 h after the last injection and subject to real-time PCR (top panel) and ELISA analyses (low panel) of TIMP-1. The values from vehicle-treated group were set as 1 fold in real-time PCR analyses. Values represent means ± SEM (n = 4). * P
    Figure Legend Snippet: Up-regulation of hepatic and serum TIMP-1 levels following CCl 4 -induced acute or chronic liver injury in mice . A , Mice were treated with a single dose of CCl 4 injection or vehicle (olive oil) injection. Liver tissues were then collected and subject to real-time PCR analysis of hepatic TIMP-1 mRNA (top panel). Sera were collected for measurement of TIMP-1 protein by ELISA (low panel). B , Mice were treated chronically with CCl 4 or vehicle (olive oil) for 4 weeks. Liver tissues and sera were collected 24 and 48 h after the last injection and subject to real-time PCR (top panel) and ELISA analyses (low panel) of TIMP-1. The values from vehicle-treated group were set as 1 fold in real-time PCR analyses. Values represent means ± SEM (n = 4). * P

    Techniques Used: Mouse Assay, Injection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    11) Product Images from "MicroRNA-495 inhibits the high glucose-induced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1"

    Article Title: MicroRNA-495 inhibits the high glucose-induced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

    Journal: Cellular & Molecular Biology Letters

    doi: 10.1186/s11658-018-0089-x

    Effects of miR-495 on high glucose-induced imbalance of MMP-TIMP and collagen synthesis in human CFs. CFs were transfected with miR-495 inhibitor or mimic for 48 h, and then treated with 5.5 or 25 mM glucose for 24 h. HG + miR-NC indicates CFs transfected with the miR-495 inhibitor before treatment with high glucose; HG + miR-495 mimic indicates CFs transfected with the miR-495 mimic before treatment with high glucose. a The secreted MMP-2, MMP-9 and TIMP-1 proteins in the supernatants of CFs were detected using ELISA assays. b The mRNA expressions of collagen I and III were determined via quantitative RT-PCR. The data shown are means ± SEM ( n = 6). ** p
    Figure Legend Snippet: Effects of miR-495 on high glucose-induced imbalance of MMP-TIMP and collagen synthesis in human CFs. CFs were transfected with miR-495 inhibitor or mimic for 48 h, and then treated with 5.5 or 25 mM glucose for 24 h. HG + miR-NC indicates CFs transfected with the miR-495 inhibitor before treatment with high glucose; HG + miR-495 mimic indicates CFs transfected with the miR-495 mimic before treatment with high glucose. a The secreted MMP-2, MMP-9 and TIMP-1 proteins in the supernatants of CFs were detected using ELISA assays. b The mRNA expressions of collagen I and III were determined via quantitative RT-PCR. The data shown are means ± SEM ( n = 6). ** p

    Techniques Used: Transfection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

    12) Product Images from "Photobiomodulation of extracellular matrix enzymes in human nucleus pulposus cells as a potential treatment for intervertebral disk degeneration"

    Article Title: Photobiomodulation of extracellular matrix enzymes in human nucleus pulposus cells as a potential treatment for intervertebral disk degeneration

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-30185-3

    Effects of potential contributing factors, derived from macrophages, on human NP cells with/without BAY11-7082 as an inhibitor of the nuclear factor kappa B (NF-κB) activity. ( A ) Production of IL-1β and ( B ) TNF-α; ( C ) total collagen secretion; ( D ) production of MMP-1 and ( E ) MMP-3. ( F ) Gene expression of MMP1 and ( G ) MMP3 . ( H ) Production of TIMP-1 and ( I ) TIMP-2 as endogenous inhibitors of MMP-3 and MMP-1, respectively. Values are mean ± SE of three or four independent experiments. *p
    Figure Legend Snippet: Effects of potential contributing factors, derived from macrophages, on human NP cells with/without BAY11-7082 as an inhibitor of the nuclear factor kappa B (NF-κB) activity. ( A ) Production of IL-1β and ( B ) TNF-α; ( C ) total collagen secretion; ( D ) production of MMP-1 and ( E ) MMP-3. ( F ) Gene expression of MMP1 and ( G ) MMP3 . ( H ) Production of TIMP-1 and ( I ) TIMP-2 as endogenous inhibitors of MMP-3 and MMP-1, respectively. Values are mean ± SE of three or four independent experiments. *p

    Techniques Used: Derivative Assay, Activity Assay, Expressing

    Gene and protein expression of MMP-3 and production of TIMP-1 as endogenous inhibitor of MMP-3, in NPM treated with PBM. ( A ) Production of MMP-3 at 630 nm, ( B ) 525 nm, and ( C ) 465 nm. ( D ) Relative gene expression of MMP3 at 630 nm, ( E ) 525 nm, and ( F ) 465 nm. ( G ) Production of TIMP-1 at 630 nm, ( H ) 525 nm, and ( I ) 465 nm. Values are mean ± SE of three or four independent experiments. *p
    Figure Legend Snippet: Gene and protein expression of MMP-3 and production of TIMP-1 as endogenous inhibitor of MMP-3, in NPM treated with PBM. ( A ) Production of MMP-3 at 630 nm, ( B ) 525 nm, and ( C ) 465 nm. ( D ) Relative gene expression of MMP3 at 630 nm, ( E ) 525 nm, and ( F ) 465 nm. ( G ) Production of TIMP-1 at 630 nm, ( H ) 525 nm, and ( I ) 465 nm. Values are mean ± SE of three or four independent experiments. *p

    Techniques Used: Expressing

    13) Product Images from "uPA and uPAR shRNA Inhibit Angiogenesis via Enhanced Secretion of SVEGFR1 Independent of GM-CSF but Dependent on TIMP-1 in Endothelial and Glioblastoma Cells"

    Article Title: uPA and uPAR shRNA Inhibit Angiogenesis via Enhanced Secretion of SVEGFR1 Independent of GM-CSF but Dependent on TIMP-1 in Endothelial and Glioblastoma Cells

    Journal: Molecular Oncology

    doi: 10.1016/j.molonc.2011.11.008

    shRNA against uPA and uPAR enhances secretion of TIMP‐1 from HMEC, U87MG and 4910 cells by Western Blot and ELISA. (A) (Upper panel) 1.5×105 HMEC, U87MG and 4910 cells were transfected as described earlier. Conditioned medium was collected
    Figure Legend Snippet: shRNA against uPA and uPAR enhances secretion of TIMP‐1 from HMEC, U87MG and 4910 cells by Western Blot and ELISA. (A) (Upper panel) 1.5×105 HMEC, U87MG and 4910 cells were transfected as described earlier. Conditioned medium was collected

    Techniques Used: shRNA, Western Blot, Enzyme-linked Immunosorbent Assay, Transfection

    14) Product Images from "Fibroblast Response to Gadolinium: Role for Platelet-Derived Growth Factor Receptor"

    Article Title: Fibroblast Response to Gadolinium: Role for Platelet-Derived Growth Factor Receptor

    Journal: Investigative radiology

    doi: 10.1097/RLI.0b013e3181e943d2

    Effects of U0126 and LY294002 on proliferation, MMP-1 production and TIMP-1 production in Gd 3+ - treated fibroblasts
    Figure Legend Snippet: Effects of U0126 and LY294002 on proliferation, MMP-1 production and TIMP-1 production in Gd 3+ - treated fibroblasts

    Techniques Used:

    15) Product Images from "CIGARETTE SMOKE ALTERS TIMP-1 AND MMP-9 LEVELS IN THE BASOLATERAL SECRETIONS OF HUMAN ASTHMATIC BRONCHIAL EPITHELIUM IN VITRO"

    Article Title: CIGARETTE SMOKE ALTERS TIMP-1 AND MMP-9 LEVELS IN THE BASOLATERAL SECRETIONS OF HUMAN ASTHMATIC BRONCHIAL EPITHELIUM IN VITRO

    Journal: Journal of Investigative Medicine

    doi: 10.231/JIM.0b013e3181db874e

    Comparison of TIMP-1 and MMP-9 protein expression in basolateral secretions of primary human differentiated normal (n=4) and asthmatic (n=4) respiratory epithelium in vitro following exposure to H 2 O 2 or CSC. Epithelia were cultured for 23 hours after a 1 h exposure to H 2 O 2 or PBS (vehicle) and CSC or DMSO (vehicle). TIMP-1 (A) and MMP-9 (B) protein levels in basolateral secretions were measured by ELISA and the data used to determine the MMP-9:TIMP-1 ratio (C). Horizontal lines indicate statistical comparisons between underlying groups for which p-values are *p≤0.05 and ** p≤0.01. Experiments were done in tissues from 4 pairs of patients and run in triplicate wells.
    Figure Legend Snippet: Comparison of TIMP-1 and MMP-9 protein expression in basolateral secretions of primary human differentiated normal (n=4) and asthmatic (n=4) respiratory epithelium in vitro following exposure to H 2 O 2 or CSC. Epithelia were cultured for 23 hours after a 1 h exposure to H 2 O 2 or PBS (vehicle) and CSC or DMSO (vehicle). TIMP-1 (A) and MMP-9 (B) protein levels in basolateral secretions were measured by ELISA and the data used to determine the MMP-9:TIMP-1 ratio (C). Horizontal lines indicate statistical comparisons between underlying groups for which p-values are *p≤0.05 and ** p≤0.01. Experiments were done in tissues from 4 pairs of patients and run in triplicate wells.

    Techniques Used: Expressing, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay

    16) Product Images from "Therapeutic administration of orlistat, rosiglitazone or the chemokine receptor antagonist RS102895 fails to improve the severity of acute pancreatitis in obese mice"

    Article Title: Therapeutic administration of orlistat, rosiglitazone or the chemokine receptor antagonist RS102895 fails to improve the severity of acute pancreatitis in obese mice

    Journal: Pancreas

    doi: 10.1097/MPA.0000000000000115

    Concentrations of A) adiponectin and B) TIMP-1 at day 3 of acute pancreatitis in obese mice administered DMSO (vehicle), orlistat, rosiglitazone, or RS102895. Data are expressed means ± SEM of 7 mice per group. * p
    Figure Legend Snippet: Concentrations of A) adiponectin and B) TIMP-1 at day 3 of acute pancreatitis in obese mice administered DMSO (vehicle), orlistat, rosiglitazone, or RS102895. Data are expressed means ± SEM of 7 mice per group. * p

    Techniques Used: Mouse Assay

    17) Product Images from "Toll-Like Receptors 2 and 4 Are Potential Therapeutic Targets in Peritoneal Dialysis–Associated Fibrosis"

    Article Title: Toll-Like Receptors 2 and 4 Are Potential Therapeutic Targets in Peritoneal Dialysis–Associated Fibrosis

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2015080923

    Synergism between TLRs and C5aR in peritoneal macrophages but not in peritoneal mesothelial cells enhances pro– and anti–inflammatory and fibrotic mediator release. Levels of proinflammatory cytokines and fibrotic markers in the culture supernatants of (A, C, and D) PDE–isolated resident peritoneal leukocytes or (B) peritoneal mesothelial cells (from omentum) stimulated overnight with the indicated concentrations of Pam 3 Cys (or 250 ng/ml), LPS (or 1 ng/ml), or heat–killed E. coli in the presence or absence of increasing concentrations of C5a. In C, cells were preincubated with an anti–C5L2 blocking mAb (1D9; 5 μ g/ml). IL-8, IL-13, TGF- β , and TIMP-1 levels were determined by ELISA, and IL-6, IL-10, TNF- α , MMP-1, MMP-3, and MMP-9 levels were determined by multiplex ELISA (Meso Scale Discovery). Results are from one experiment (±SD) representative of (A and B) 10 or (C and D) five performed with cells from different donors. The P values indicate statistical significance for the comparison between the additive response to a TLR agonist alone and C5a alone and the response to the combination of TLR agonist and C5a. * P
    Figure Legend Snippet: Synergism between TLRs and C5aR in peritoneal macrophages but not in peritoneal mesothelial cells enhances pro– and anti–inflammatory and fibrotic mediator release. Levels of proinflammatory cytokines and fibrotic markers in the culture supernatants of (A, C, and D) PDE–isolated resident peritoneal leukocytes or (B) peritoneal mesothelial cells (from omentum) stimulated overnight with the indicated concentrations of Pam 3 Cys (or 250 ng/ml), LPS (or 1 ng/ml), or heat–killed E. coli in the presence or absence of increasing concentrations of C5a. In C, cells were preincubated with an anti–C5L2 blocking mAb (1D9; 5 μ g/ml). IL-8, IL-13, TGF- β , and TIMP-1 levels were determined by ELISA, and IL-6, IL-10, TNF- α , MMP-1, MMP-3, and MMP-9 levels were determined by multiplex ELISA (Meso Scale Discovery). Results are from one experiment (±SD) representative of (A and B) 10 or (C and D) five performed with cells from different donors. The P values indicate statistical significance for the comparison between the additive response to a TLR agonist alone and C5a alone and the response to the combination of TLR agonist and C5a. * P

    Techniques Used: Isolation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Multiplex Assay

    18) Product Images from "Matrix Metalloproteinases and Their Inhibitors: Correlation with Invasion and Metastasis in Oral Cancer"

    Article Title: Matrix Metalloproteinases and Their Inhibitors: Correlation with Invasion and Metastasis in Oral Cancer

    Journal: Indian Journal of Clinical Biochemistry

    doi: 10.1007/s12291-010-0060-8

    ROC curve for MMP-2, MMP-9, TIMP-1 and TIMP-2 between oral cancer patients and healthy controls for the results obtained by ELISA
    Figure Legend Snippet: ROC curve for MMP-2, MMP-9, TIMP-1 and TIMP-2 between oral cancer patients and healthy controls for the results obtained by ELISA

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Representative pattern for mRNA expression of MMP-2, MMP-9, TIMP-2 and TIMP-1 by RT-PCR. Lanes 1 , 4 , 7 adjacent normal tissues; Lanes 2 , 5 , 8 malignant tissues; Lane 6 molecular weight marker; Lane 3 , 9 negative control
    Figure Legend Snippet: Representative pattern for mRNA expression of MMP-2, MMP-9, TIMP-2 and TIMP-1 by RT-PCR. Lanes 1 , 4 , 7 adjacent normal tissues; Lanes 2 , 5 , 8 malignant tissues; Lane 6 molecular weight marker; Lane 3 , 9 negative control

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker, Negative Control

    19) Product Images from "Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism"

    Article Title: Chondroprotective effect of curcumin and lecithin complex in human chondrocytes stimulated by IL-1β via an anti-inflammatory mechanism

    Journal: Food Science and Biotechnology

    doi: 10.1007/s10068-018-0470-6

    Comparison of COL2 ( A ), PG ( B ), HA ( C ), and TIMP-1 ( D ) in HCH-c cells stimulated with IL-1β. Cells were seeded at 1.0 × 10 5 cells/mL, then stimulated with IL-1β and sample for 48 h. * Significant different from the IL-1β values by ANOVA followed by Dunnett’s test ( p
    Figure Legend Snippet: Comparison of COL2 ( A ), PG ( B ), HA ( C ), and TIMP-1 ( D ) in HCH-c cells stimulated with IL-1β. Cells were seeded at 1.0 × 10 5 cells/mL, then stimulated with IL-1β and sample for 48 h. * Significant different from the IL-1β values by ANOVA followed by Dunnett’s test ( p

    Techniques Used:

    20) Product Images from "TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice"

    Article Title: TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-00671-1

    TIMP-1 deficiency does not prevent hepatic fibrogenesis following CCl 4 treatment. ( a ) Collagen content of livers following chronic CCl 4 treatment was assessed by Sirius Red staining. TIMP-1 deficiency does not alter collagen content compared to TIMP-1 wt, as shown in representative images and ( c ) in quantification of Sirius Red positive stained area. ( d ) Supportively hepatic hydroxyproline content also did not differ between TIMP-1 deficient and wildtype animals following CCl 4 treatment ( b , e ) αSMA IHC indicates hepatic stellate cell activation and was increased in livers following CCl 4 administration. TIMP-1 ko have the same amount of αSMA positive cells compared to TIMP-1 wt. ( f ) Fibrosis-related genes were upregulated in livers after CCl 4 treatment, but no differences could be observed between TIMP-1 wt and TIMP-1 ko mice. Not only MMP-9 gene expression was not altered, but ( g ) also Western blot analysis of MMP-9 protein showed no differences in TIMP-1 ko livers compared to wildtypes. Displayed are cropped images. Full-length blots are presented in Supplementary Figure 3 . Bar columns represent mean ± standard error of the mean. **p
    Figure Legend Snippet: TIMP-1 deficiency does not prevent hepatic fibrogenesis following CCl 4 treatment. ( a ) Collagen content of livers following chronic CCl 4 treatment was assessed by Sirius Red staining. TIMP-1 deficiency does not alter collagen content compared to TIMP-1 wt, as shown in representative images and ( c ) in quantification of Sirius Red positive stained area. ( d ) Supportively hepatic hydroxyproline content also did not differ between TIMP-1 deficient and wildtype animals following CCl 4 treatment ( b , e ) αSMA IHC indicates hepatic stellate cell activation and was increased in livers following CCl 4 administration. TIMP-1 ko have the same amount of αSMA positive cells compared to TIMP-1 wt. ( f ) Fibrosis-related genes were upregulated in livers after CCl 4 treatment, but no differences could be observed between TIMP-1 wt and TIMP-1 ko mice. Not only MMP-9 gene expression was not altered, but ( g ) also Western blot analysis of MMP-9 protein showed no differences in TIMP-1 ko livers compared to wildtypes. Displayed are cropped images. Full-length blots are presented in Supplementary Figure 3 . Bar columns represent mean ± standard error of the mean. **p

    Techniques Used: Staining, Immunohistochemistry, Activation Assay, Mouse Assay, Expressing, Western Blot

    TIMP-1 deficient mice are not protected from hepatic carcinogenesis. Liver carcinogenesis was induced via juvenile DEN injection. ( a ) No differences between TIMP-1 wt and ko could be observed in tumor number or size. ( b ) TIMP-1 wt and ko show the same liverweight/bodyweight ratio. ( c ) ALT level were not induced following DEN treatment and did not differ between TIMP-1 wt and TIMP-1 ko. ( d ) Representative pictures of excised livers and ( e ) in vivo MRI images show equal tumor load of livers. Bar columns represent mean ± standard error of the mean. (n = 16–22).
    Figure Legend Snippet: TIMP-1 deficient mice are not protected from hepatic carcinogenesis. Liver carcinogenesis was induced via juvenile DEN injection. ( a ) No differences between TIMP-1 wt and ko could be observed in tumor number or size. ( b ) TIMP-1 wt and ko show the same liverweight/bodyweight ratio. ( c ) ALT level were not induced following DEN treatment and did not differ between TIMP-1 wt and TIMP-1 ko. ( d ) Representative pictures of excised livers and ( e ) in vivo MRI images show equal tumor load of livers. Bar columns represent mean ± standard error of the mean. (n = 16–22).

    Techniques Used: Mouse Assay, Injection, In Vivo, Magnetic Resonance Imaging

    Confirmation of TIMP-1 protein deletion in hepatic stellate cells from TIMP-1 ko mice . ( a ) Cropped Western Blots showing no TIMP-1 protein in quiescent stellate cell (qHSC) lysates and activated stellate cells (aHSC) isolated from TIMP-1 ko mice, whereas activated stellate cells from TIMP-1 wt livers show elevated TIMP-1 protein level. Activation status of hepatic stellate cells is indicated by αSMA expression. Each lane represents lysed HSCs pooled after isolation from 3 mice. Full-length blots are presented in Supplementary Figure 3 .
    Figure Legend Snippet: Confirmation of TIMP-1 protein deletion in hepatic stellate cells from TIMP-1 ko mice . ( a ) Cropped Western Blots showing no TIMP-1 protein in quiescent stellate cell (qHSC) lysates and activated stellate cells (aHSC) isolated from TIMP-1 ko mice, whereas activated stellate cells from TIMP-1 wt livers show elevated TIMP-1 protein level. Activation status of hepatic stellate cells is indicated by αSMA expression. Each lane represents lysed HSCs pooled after isolation from 3 mice. Full-length blots are presented in Supplementary Figure 3 .

    Techniques Used: Mouse Assay, Western Blot, Isolation, Activation Assay, Expressing

    TIMP-1 deficient mice are not protected from fibrosis-driven hepatic carcinogenesis. ( a ) TIMP-1 expression increased in HCC tissue compared to adjacent paired normal tissue and control liver tissue following juvenile DEN administration and chronic CCl 4 injection. ( b ) Tumorload does not differ between TIMP-1 wt and ko as assessed by tumor number and size and ( e ) visualized by representative pictures of excised livers and ( f ) in vivo MRI images. ( c ) TIMP-1-deficiency does not alter the liverweight/bodyweight ratio. ( d ) A combination of juvenile DEN administration and chronic CCl 4 treatment led to overall increased ALT level, whereas elevated level could be measured in serum from TIMP-1 ko mice as compared with TIMP-1 wt. Bar columns represent mean ± standard error of the mean. **p
    Figure Legend Snippet: TIMP-1 deficient mice are not protected from fibrosis-driven hepatic carcinogenesis. ( a ) TIMP-1 expression increased in HCC tissue compared to adjacent paired normal tissue and control liver tissue following juvenile DEN administration and chronic CCl 4 injection. ( b ) Tumorload does not differ between TIMP-1 wt and ko as assessed by tumor number and size and ( e ) visualized by representative pictures of excised livers and ( f ) in vivo MRI images. ( c ) TIMP-1-deficiency does not alter the liverweight/bodyweight ratio. ( d ) A combination of juvenile DEN administration and chronic CCl 4 treatment led to overall increased ALT level, whereas elevated level could be measured in serum from TIMP-1 ko mice as compared with TIMP-1 wt. Bar columns represent mean ± standard error of the mean. **p

    Techniques Used: Mouse Assay, Expressing, Injection, In Vivo, Magnetic Resonance Imaging

    TIMP-1 deficiency does not prevent hepatic fibrogenesis in the BDL model. ( a ) Collagen content of livers 21d post BDL was assessed by Sirius Red staining. TIMP-1 deficiency does not alter Collagen content compared to TIMP-1 wt, as shown in representative images and in ( b ) quantification of Sirius Red positive stained area. ( c ) Hepatic hydroxyproline content shows also an induction after BDL treatment, but no differences between TIMP-1 ko and wt. ( d , e ) αSMA IHC indicates hepatic stellate cell activation and was increased in livers following 21d of BDL. TIMP-1 ko have the same amount of αSMA positive cells compared to TIMP-1 wt. ( f ) Liver transaminases (ALT) were elevated following BDL but did not differ significantly between TIMP-1 wt and TIMP-1 ko . ( g ) Fibrosis-related genes were upregulated in BDL livers, but no differences could be observed between TIMP-1 wt and TIMP-1 ko mice. Bar columns represent mean ± standard error of the mean. **p
    Figure Legend Snippet: TIMP-1 deficiency does not prevent hepatic fibrogenesis in the BDL model. ( a ) Collagen content of livers 21d post BDL was assessed by Sirius Red staining. TIMP-1 deficiency does not alter Collagen content compared to TIMP-1 wt, as shown in representative images and in ( b ) quantification of Sirius Red positive stained area. ( c ) Hepatic hydroxyproline content shows also an induction after BDL treatment, but no differences between TIMP-1 ko and wt. ( d , e ) αSMA IHC indicates hepatic stellate cell activation and was increased in livers following 21d of BDL. TIMP-1 ko have the same amount of αSMA positive cells compared to TIMP-1 wt. ( f ) Liver transaminases (ALT) were elevated following BDL but did not differ significantly between TIMP-1 wt and TIMP-1 ko . ( g ) Fibrosis-related genes were upregulated in BDL livers, but no differences could be observed between TIMP-1 wt and TIMP-1 ko mice. Bar columns represent mean ± standard error of the mean. **p

    Techniques Used: Staining, Immunohistochemistry, Activation Assay, Mouse Assay

    21) Product Images from "Hypoxia induces a phenotypic switch of fibroblasts to myofibroblasts through a MMP-2/TIMP mediated pathway: Implications for venous neointimal hyperplasia in hemodialysis access"

    Article Title: Hypoxia induces a phenotypic switch of fibroblasts to myofibroblasts through a MMP-2/TIMP mediated pathway: Implications for venous neointimal hyperplasia in hemodialysis access

    Journal: Journal of vascular and interventional radiology : JVIR

    doi: 10.1016/j.jvir.2010.02.030

    Immunoprecipitation followed by Western blot analysis of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) by hypoxic and normoxic fibroblasts at different time points. Upper panel is representative blots of TIMP-1 by normoxic (N), 24 hour (24 h), 48 hour (48 h), and 72 hour (72 h) fibroblasts and controls with IgG loading. Lower panel shows pooled data for TIMP-1. The expression of TIMP-1 (*) was significantly higher by the hypoxic fibroblasts than the normoxic fibroblasts at all time points (P
    Figure Legend Snippet: Immunoprecipitation followed by Western blot analysis of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) by hypoxic and normoxic fibroblasts at different time points. Upper panel is representative blots of TIMP-1 by normoxic (N), 24 hour (24 h), 48 hour (48 h), and 72 hour (72 h) fibroblasts and controls with IgG loading. Lower panel shows pooled data for TIMP-1. The expression of TIMP-1 (*) was significantly higher by the hypoxic fibroblasts than the normoxic fibroblasts at all time points (P

    Techniques Used: Immunoprecipitation, Western Blot, Expressing

    22) Product Images from "Lymphocyte/Macrophage Interactions: Biomaterial Surface-Dependent Cytokine, Chemokine, and Matrix Protein Production"

    Article Title: Lymphocyte/Macrophage Interactions: Biomaterial Surface-Dependent Cytokine, Chemokine, and Matrix Protein Production

    Journal:

    doi: 10.1002/jbm.a.31630

    Ratio of MMP-9/TIMP-1 in co-cultures over (A) 3 days, (C) 7 days, and (E) 10 days. Ratio of MMP-9/TIMP-2 in co-cultures over (B) 3 days, (D) 7 days, and (F) 10 days. Data represents the mean ± standard error of the mean (SEM), n = 4. *Significance
    Figure Legend Snippet: Ratio of MMP-9/TIMP-1 in co-cultures over (A) 3 days, (C) 7 days, and (E) 10 days. Ratio of MMP-9/TIMP-2 in co-cultures over (B) 3 days, (D) 7 days, and (F) 10 days. Data represents the mean ± standard error of the mean (SEM), n = 4. *Significance

    Techniques Used:

    MMP-9 production from (A) low and (B) high monocyte co-cultures over 10 days. TIMP-1 production from (C) low and (D) high monocyte co-cultures over 10 days. TIMP-2 production from (E) low and (F) high monocyte co-cultures over 10 days. Data represents
    Figure Legend Snippet: MMP-9 production from (A) low and (B) high monocyte co-cultures over 10 days. TIMP-1 production from (C) low and (D) high monocyte co-cultures over 10 days. TIMP-2 production from (E) low and (F) high monocyte co-cultures over 10 days. Data represents

    Techniques Used:

    23) Product Images from "Serum concentrations of endothelial cell adhesion molecules and their shedding enzymes and early onset sepsis in newborns in Suriname"

    Article Title: Serum concentrations of endothelial cell adhesion molecules and their shedding enzymes and early onset sepsis in newborns in Suriname

    Journal: BMJ Paediatrics Open

    doi: 10.1136/bmjpo-2018-000312

    Circulating levels of MMP-9 and TIMP-1, and TIMP-1/MMP-9 ratios in Surinamese newborns. (A) MMP-9; (B) TIMP-1; (C) TIMP-1/MMP-9 ratios. Bars represent median values and error bars 95% CI. P
    Figure Legend Snippet: Circulating levels of MMP-9 and TIMP-1, and TIMP-1/MMP-9 ratios in Surinamese newborns. (A) MMP-9; (B) TIMP-1; (C) TIMP-1/MMP-9 ratios. Bars represent median values and error bars 95% CI. P

    Techniques Used:

    24) Product Images from "Hyperphosphataemia sensitizes renally impaired rats to the profibrotic effects of gadodiamide"

    Article Title: Hyperphosphataemia sensitizes renally impaired rats to the profibrotic effects of gadodiamide

    Journal: British Journal of Pharmacology

    doi: 10.1111/j.1476-5381.2011.01585.x

    Histological (haematoxylin-eosin-saffron [HES]), and immunohistochemical (ED-1, TGFβ 1 , prolyl-4-hydroxylase, TIMP-1) analysis of dorsal skin in SNx rats fed a high-phosphate diet (typical examples from Study 2). Bands of inflammatory dermal fibrosis
    Figure Legend Snippet: Histological (haematoxylin-eosin-saffron [HES]), and immunohistochemical (ED-1, TGFβ 1 , prolyl-4-hydroxylase, TIMP-1) analysis of dorsal skin in SNx rats fed a high-phosphate diet (typical examples from Study 2). Bands of inflammatory dermal fibrosis

    Techniques Used: Immunohistochemistry

    25) Product Images from "A Collagen-based Scaffold Delivering Exogenous MicroRNA-29B to Modulate Extracellular Matrix Remodeling"

    Article Title: A Collagen-based Scaffold Delivering Exogenous MicroRNA-29B to Modulate Extracellular Matrix Remodeling

    Journal: Molecular Therapy

    doi: 10.1038/mt.2013.288

    Quantitation of wound healing factors following treatments with RNA releasing collagen scaffolds in vivo. ( a ) Fold change of TGF-β 1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. ( b ) Fold change of TIMP-1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. ( c ) Fold change of MMP-8 expression in excised tissue at day 28. ( d ) Ratios of MMP-8 to TIMP-1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. NT indicates an excisional wound with no treatment applied (NT). Data presented is the mean of n = 4 ± SD analyzed by one-way ANOVA and Tukey's post hoc test. * indicates statistical significance between the groups indicated ( P
    Figure Legend Snippet: Quantitation of wound healing factors following treatments with RNA releasing collagen scaffolds in vivo. ( a ) Fold change of TGF-β 1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. ( b ) Fold change of TIMP-1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. ( c ) Fold change of MMP-8 expression in excised tissue at day 28. ( d ) Ratios of MMP-8 to TIMP-1 expression in excised tissue at day 28, data normalized to healthy unwounded skin. NT indicates an excisional wound with no treatment applied (NT). Data presented is the mean of n = 4 ± SD analyzed by one-way ANOVA and Tukey's post hoc test. * indicates statistical significance between the groups indicated ( P

    Techniques Used: Quantitation Assay, In Vivo, Expressing

    26) Product Images from "Study of Bone Marrow and Embryonic Stem Cell-Derived Human Mesenchymal Stem Cells for Treatment of Escherichia coli Endotoxin-Induced Acute Lung Injury in Mice"

    Article Title: Study of Bone Marrow and Embryonic Stem Cell-Derived Human Mesenchymal Stem Cells for Treatment of Escherichia coli Endotoxin-Induced Acute Lung Injury in Mice

    Journal: Stem Cells Translational Medicine

    doi: 10.5966/sctm.2015-0006

    Effect of human BM-MSCs and ES-MSCs on the expression of human and mouse MMP-9 and TIMP-1 levels. (A – C): Endotoxin instillation increased BAL fluid levels of mouse total MMP-9 (both pro-MMP-9 and active-MMP-9). Mouse MMP-9 level was significantly
    Figure Legend Snippet: Effect of human BM-MSCs and ES-MSCs on the expression of human and mouse MMP-9 and TIMP-1 levels. (A – C): Endotoxin instillation increased BAL fluid levels of mouse total MMP-9 (both pro-MMP-9 and active-MMP-9). Mouse MMP-9 level was significantly

    Techniques Used: Expressing

    27) Product Images from "High levels of serum VEGF and TIMP-1 are correlated with colon cancer liver metastasis and intrahepatic recurrence after liver resection"

    Article Title: High levels of serum VEGF and TIMP-1 are correlated with colon cancer liver metastasis and intrahepatic recurrence after liver resection

    Journal: Oncology Letters

    doi: 10.3892/ol.2012.691

    Disease-free survival curves according to the levels of (A) VEGF and (B) TIMP-1.
    Figure Legend Snippet: Disease-free survival curves according to the levels of (A) VEGF and (B) TIMP-1.

    Techniques Used:

    (A) Bar chart of serum levels of MMP-7, E-selectin and osteopontin. (B) Bar chart of serum levels of VEGF and HGF. (C) Bar chart of serum levels of CEA and TIMP-1. Numbers in rectangles are the P-values.
    Figure Legend Snippet: (A) Bar chart of serum levels of MMP-7, E-selectin and osteopontin. (B) Bar chart of serum levels of VEGF and HGF. (C) Bar chart of serum levels of CEA and TIMP-1. Numbers in rectangles are the P-values.

    Techniques Used:

    28) Product Images from "Role of Angiogenic Factors in Airway Remodeling in an Allergic Rhinitis Murine Model"

    Article Title: Role of Angiogenic Factors in Airway Remodeling in an Allergic Rhinitis Murine Model

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2012.4.1.37

    Anti-angiogenic drug treatment significantly reduces tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in the nasal septum. Nasal septum sections harvested from mice at (A) 0 or (B) 3 months were subjected to immunohistochemical staining for TIMP-1, which was detected using confocal microscopy. Images were taken at ×400 magnification. Blue, 4',6-diamidino-2-phenylindole; red, TIMP-1. (C) Areas that stained positive for TIMP-1 are reported for each group of mice as percentages of positive-stained area/whole area. Group A, phosphate-buffered saline treated; group B, inhalation of ovalbumin (OVA) treated; group C, dimethyl sulfoxide/OVA treated; group D1, OVA/SU1498 treated; group D2, OVA/AG1296 treated; group D3, OVA/SU1498/AG1296 treated. * P
    Figure Legend Snippet: Anti-angiogenic drug treatment significantly reduces tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in the nasal septum. Nasal septum sections harvested from mice at (A) 0 or (B) 3 months were subjected to immunohistochemical staining for TIMP-1, which was detected using confocal microscopy. Images were taken at ×400 magnification. Blue, 4',6-diamidino-2-phenylindole; red, TIMP-1. (C) Areas that stained positive for TIMP-1 are reported for each group of mice as percentages of positive-stained area/whole area. Group A, phosphate-buffered saline treated; group B, inhalation of ovalbumin (OVA) treated; group C, dimethyl sulfoxide/OVA treated; group D1, OVA/SU1498 treated; group D2, OVA/AG1296 treated; group D3, OVA/SU1498/AG1296 treated. * P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Confocal Microscopy

    29) Product Images from "Role of Angiogenic Factors in Airway Remodeling in an Allergic Rhinitis Murine Model"

    Article Title: Role of Angiogenic Factors in Airway Remodeling in an Allergic Rhinitis Murine Model

    Journal: Allergy, Asthma & Immunology Research

    doi: 10.4168/aair.2012.4.1.37

    Anti-angiogenic drug treatment significantly reduces tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in the nasal septum. Nasal septum sections harvested from mice at (A) 0 or (B) 3 months were subjected to immunohistochemical staining for TIMP-1, which was detected using confocal microscopy. Images were taken at ×400 magnification. Blue, 4',6-diamidino-2-phenylindole; red, TIMP-1. (C) Areas that stained positive for TIMP-1 are reported for each group of mice as percentages of positive-stained area/whole area. Group A, phosphate-buffered saline treated; group B, inhalation of ovalbumin (OVA) treated; group C, dimethyl sulfoxide/OVA treated; group D1, OVA/SU1498 treated; group D2, OVA/AG1296 treated; group D3, OVA/SU1498/AG1296 treated. * P
    Figure Legend Snippet: Anti-angiogenic drug treatment significantly reduces tissue inhibitor of metalloproteinase-1 (TIMP-1) expression in the nasal septum. Nasal septum sections harvested from mice at (A) 0 or (B) 3 months were subjected to immunohistochemical staining for TIMP-1, which was detected using confocal microscopy. Images were taken at ×400 magnification. Blue, 4',6-diamidino-2-phenylindole; red, TIMP-1. (C) Areas that stained positive for TIMP-1 are reported for each group of mice as percentages of positive-stained area/whole area. Group A, phosphate-buffered saline treated; group B, inhalation of ovalbumin (OVA) treated; group C, dimethyl sulfoxide/OVA treated; group D1, OVA/SU1498 treated; group D2, OVA/AG1296 treated; group D3, OVA/SU1498/AG1296 treated. * P

    Techniques Used: Expressing, Mouse Assay, Immunohistochemistry, Staining, Confocal Microscopy

    30) Product Images from "Circulating TIMP-1 is associated with hematoma volume in patients with spontaneous intracranial hemorrhage"

    Article Title: Circulating TIMP-1 is associated with hematoma volume in patients with spontaneous intracranial hemorrhage

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-67250-9

    Fixed effect meta-analysis to assess the effect of admission TIMP-1 and hematoma volume in ICH patients. Beta coefficients were adjusted for location, hypertension and NIHSS in both cohorts (Cohort 1 = CHN and Cohort 2 = VdH).
    Figure Legend Snippet: Fixed effect meta-analysis to assess the effect of admission TIMP-1 and hematoma volume in ICH patients. Beta coefficients were adjusted for location, hypertension and NIHSS in both cohorts (Cohort 1 = CHN and Cohort 2 = VdH).

    Techniques Used:

    TIMP-1 effects in mice experimental tail bleeding. ( a ) Bleeding time of animals treated with saline (n = 5), TIMP-1 (0.05 mg/Kg, n = 4) and TIMP-1 (0.2 mg/Kg, n = 3). ( b ) Blood lost obtained after experimental bleeding model. **p
    Figure Legend Snippet: TIMP-1 effects in mice experimental tail bleeding. ( a ) Bleeding time of animals treated with saline (n = 5), TIMP-1 (0.05 mg/Kg, n = 4) and TIMP-1 (0.2 mg/Kg, n = 3). ( b ) Blood lost obtained after experimental bleeding model. **p

    Techniques Used: Mouse Assay

    31) Product Images from "Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *"

    Article Title: Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.093799

    Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p
    Figure Legend Snippet: Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p

    Techniques Used: Western Blot, Immunoprecipitation

    TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p
    Figure Legend Snippet: TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p

    Techniques Used: Western Blot, Incubation, Activity Assay

    32) Product Images from "Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *"

    Article Title: Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.093799

    Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p
    Figure Legend Snippet: Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p

    Techniques Used: Western Blot, Immunoprecipitation

    TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p
    Figure Legend Snippet: TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p

    Techniques Used: Western Blot, Incubation, Activity Assay

    33) Product Images from "Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *"

    Article Title: Matrix Metalloproteinase-3 Is Increased and Participates in Neuronal Apoptotic Signaling Downstream of Caspase-12 during Endoplasmic Reticulum Stress *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.093799

    Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p
    Figure Legend Snippet: Caspase-12 can degrade TIMP-1 that is bound to MMP-3. A , typical Western blot against MMP-3 on lysate of CATH.a cells that has been exposed to caspase-12 and immunoprecipitated with TIMP-1 antibody. The intensity of the MMP-3 band was determined by densitometry and expressed as -fold induction relative to the untreated control. ***, p

    Techniques Used: Western Blot, Immunoprecipitation

    TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p
    Figure Legend Snippet: TIMP-1 is a substrate of caspase-12. A , typical Western blot against TIMP-1 following incubation of 5 ng of caspase-12 with 3 ng of TIMP-1 for various time periods. B , MMP-3 enzyme activity of 5 ng of cMMP-3 measured in the presence of 3 ng of TIMP-1 with or without prior overnight exposure to 5 ng of caspase-12. **, p

    Techniques Used: Western Blot, Incubation, Activity Assay

    34) Product Images from "The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue"

    Article Title: The Ras guanine nucleotide exchange factor RasGRF1 promotes matrix metalloproteinase-3 production in rheumatoid arthritis synovial tissue

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar2785

    Effect of RasGRF1 overexpression on rheumatoid arthritis fibroblast-like synoviocyte matrix metalloproteinase and cytokine production. Tissue culture supernatants from rheumatoid arthritis fibroblast-like synoviocytes transfected with empty vector or with Ras guanine nucleotide-releasing factor 1 (RasGRF1) were harvested and assessed for production of (a) matrix metalloproteinase (MMP)-1, (b) TIMP-1, (c) the ratio of TIMP-1 to MMP-1, (d) MMP-3, (e) IL-6 (n = 4 each) and (f) IL-8 (n = 3) by ELISA. * P
    Figure Legend Snippet: Effect of RasGRF1 overexpression on rheumatoid arthritis fibroblast-like synoviocyte matrix metalloproteinase and cytokine production. Tissue culture supernatants from rheumatoid arthritis fibroblast-like synoviocytes transfected with empty vector or with Ras guanine nucleotide-releasing factor 1 (RasGRF1) were harvested and assessed for production of (a) matrix metalloproteinase (MMP)-1, (b) TIMP-1, (c) the ratio of TIMP-1 to MMP-1, (d) MMP-3, (e) IL-6 (n = 4 each) and (f) IL-8 (n = 3) by ELISA. * P

    Techniques Used: Over Expression, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

    Effect of RasGRF1 gene silencing on rheumatoid arthritis fibroblast-like synoviocyte matrix metalloproteinase and cytokine production. Tissue culture supernatants from rheumatoid arthritis fibroblast-like synoviocytes treated with transfection reagent alone (mock) or transfected with control or Ras guanine nucleotide-releasing factor 1 (RasGRF1) locked nucleic acid (LNA) were harvested and assessed for production of (a) matrix metalloproteinase (MMP)-1, (b) TIMP-1, (c) the ratio of TIMP-1 to MMP-1, (d) MMP-3, (e) IL-6 (n = 4 each) and (f) IL-8 (n = 3) by ELISA. * P
    Figure Legend Snippet: Effect of RasGRF1 gene silencing on rheumatoid arthritis fibroblast-like synoviocyte matrix metalloproteinase and cytokine production. Tissue culture supernatants from rheumatoid arthritis fibroblast-like synoviocytes treated with transfection reagent alone (mock) or transfected with control or Ras guanine nucleotide-releasing factor 1 (RasGRF1) locked nucleic acid (LNA) were harvested and assessed for production of (a) matrix metalloproteinase (MMP)-1, (b) TIMP-1, (c) the ratio of TIMP-1 to MMP-1, (d) MMP-3, (e) IL-6 (n = 4 each) and (f) IL-8 (n = 3) by ELISA. * P

    Techniques Used: Transfection, Enzyme-linked Immunosorbent Assay

    35) Product Images from "Kinetics of Serum Cytokines after Primary or Repeat Vaccination with the Smallpox Vaccine"

    Article Title: Kinetics of Serum Cytokines after Primary or Repeat Vaccination with the Smallpox Vaccine

    Journal: The Journal of infectious diseases

    doi: 10.1086/651453

    Percentage of vaccinees with greater than a 1.5-fold increase in cytokine levels from baseline after receiving the smallpox vaccine. Serum levels of IFN-γ, IP-10, MIG, TNF-α, sTNFR1, sTNRF2, IL-6, G-CSF (A), and levels of GM-CSF, TIMP-1,
    Figure Legend Snippet: Percentage of vaccinees with greater than a 1.5-fold increase in cytokine levels from baseline after receiving the smallpox vaccine. Serum levels of IFN-γ, IP-10, MIG, TNF-α, sTNFR1, sTNRF2, IL-6, G-CSF (A), and levels of GM-CSF, TIMP-1,

    Techniques Used:

    36) Product Images from "Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion *"

    Article Title: Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.598011

    TIMP-1 Protein Parallels Adiponectin Levels in Patients with NASH
    Figure Legend Snippet: TIMP-1 Protein Parallels Adiponectin Levels in Patients with NASH

    Techniques Used:

    Adiponectin-stimulated TIMP-1 inhibits HSC migration and FAK activity. In migration assays rat HSCs were treated with combinations of fAd (5 μg/ml), IgG, CD63, and TIMP-1 antibodies (0.25 μg/ml). A , fAd inhibited invasion 4-fold. The blocking
    Figure Legend Snippet: Adiponectin-stimulated TIMP-1 inhibits HSC migration and FAK activity. In migration assays rat HSCs were treated with combinations of fAd (5 μg/ml), IgG, CD63, and TIMP-1 antibodies (0.25 μg/ml). A , fAd inhibited invasion 4-fold. The blocking

    Techniques Used: Migration, Activity Assay, Blocking Assay

    Adiponectin promotes TIMP-1 expression and inhibits FAK activity in vivo . fAd injection into mice increased hepatic TIMP-1 expression 3-fold ( A ), elevated serum TIMP-1 in WT (not significant) ( B ), and increased the hepatic p-AMPK/AMPK ratio and reduced
    Figure Legend Snippet: Adiponectin promotes TIMP-1 expression and inhibits FAK activity in vivo . fAd injection into mice increased hepatic TIMP-1 expression 3-fold ( A ), elevated serum TIMP-1 in WT (not significant) ( B ), and increased the hepatic p-AMPK/AMPK ratio and reduced

    Techniques Used: Expressing, Activity Assay, In Vivo, Injection, Mouse Assay

    Positive correlation between serum TIMP-1 and adiponectin levels. A , ELISA revealed increased serum TIMP-1 in patients with high versus low serum adiponectin ( > 10 μg/ml serum adiponectin; n = 14; TIMP-1: 372 ± 15 ng/ml, versus
    Figure Legend Snippet: Positive correlation between serum TIMP-1 and adiponectin levels. A , ELISA revealed increased serum TIMP-1 in patients with high versus low serum adiponectin ( > 10 μg/ml serum adiponectin; n = 14; TIMP-1: 372 ± 15 ng/ml, versus

    Techniques Used: Enzyme-linked Immunosorbent Assay

    37) Product Images from "Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells"

    Article Title: Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065251

    TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Staining

    CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Quantitative RT-PCR, SDS Page, Zymography, Expressing, Immunohistochemistry, Staining

    38) Product Images from "Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells"

    Article Title: Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065251

    TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Staining

    CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Quantitative RT-PCR, SDS Page, Zymography, Expressing, Immunohistochemistry, Staining

    39) Product Images from "Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells"

    Article Title: Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065251

    TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Staining

    CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Quantitative RT-PCR, SDS Page, Zymography, Expressing, Immunohistochemistry, Staining

    40) Product Images from "Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells"

    Article Title: Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065251

    TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: TIMP-1 deficiency reduced liver fibrosis after BDL. TIMP-1 +/+ and TIMP-1 −/− mice received CBDL+CDL (A, B) or PBDL (C). The animals were sacrificed at the indicated times. (A) The injured lesions in the livers were assessed by H E staining (original magnification: 40×, left panel). Serum ALT levels were compared (right panel). (B) Survival curves for animals with CBDL+CDL. (C) Collagen deposition in the ligated left robes was assessed by Sirius red staining (original magnification: 40×). Sirius red positive area was compared (right panel). Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Staining

    CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P
    Figure Legend Snippet: CBDL+CDL increased TIMP-1 in a TNF-α dependent manner. TNF-α +/+ and TNF-α −/− mice received CBDL+CDL. The animals were sacrificed at the indicated times. (A) mRNA levels of TIMP-1 in the livers were determined by quantitative real time RT-PCR. (B) The protein extracts from the livers were analyzed by SDS-PAGE, and immunoblotting was performed with anti-TIMP-1 and -GAPDH antibodies. The results shown are representative of at least 5 independent experiments. Relative densitometric intensity of TIMP-1 was determined for each protein band and normalized to GAPDH (bottom panels). (C) Collagenase activities in the protein extracts were measured by gelatin zymography. (D) Expression of TIMP-1 in the livers was examined by immunohistochemistry (original magnification: 400×). (E) Expression of desmin (green) and TIMP-1 (red) around the interstitial space around dilated bile ducts was examined by immunofluorescent staining. The results shown are representative of at least 3 independent experiments. Data are mean ± SD from at least 5 independent experiments. *, P

    Techniques Used: Mouse Assay, Quantitative RT-PCR, SDS Page, Zymography, Expressing, Immunohistochemistry, Staining

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of Tissue Inhibitor of Metalloproteinase-1 by Astrocytes
    Article Snippet: .. In order to establish the validity of differences in the levels of GFAP or TIMP-1, results were normalized to GAPDH transcript levels as determined by the quantikine mRNA ELISA supplied by R & D Systems. .. Amplified DNAs were identified by Southern blotting.

    Article Title: Photobiomodulation of extracellular matrix enzymes in human nucleus pulposus cells as a potential treatment for intervertebral disk degeneration
    Article Snippet: .. The concentrations of IL-1β, TNF-α, MMP-1, MMP-3, TIMP-1, TIMP-2, ADAMTS-4, and ADAMTS-5 were measured in the supernatant using commercially available ELISA kits (R & D Systems) according to the manufacturers’ protocols. .. Human NP cells were lysed with Trizol reagent (Invitrogen), RNA was extracted, and cDNA synthesized (Life Technologies) according to the manufacturer’s instructions.

    Sandwich ELISA:

    Article Title: IL-6 and High Glucose Synergistically Upregulate MMP-1 Expression by U937 Mononuclear Phagocytes via ERK1/2 and JNK Pathways and c-Jun
    Article Snippet: .. MMP-1 and TIMP-1 in conditioned medium was quantified using sandwich ELISA kits according to the protocol provided by the manufacturer (R & D System, Minneapolis, MN). .. Total RNA was isolated from cells using the RNeasy minikit (Qiagen, Santa Clarita, CA).

    Incubation:

    Article Title: TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice
    Article Snippet: .. Membranes were incubated with antibodies to TIMP-1 and MMP-9 (R & D Systems, Minneapolis, MN), αSMA (Sigma-Aldrich, St. Louis, MO, USA) and, as a loading control, total actin or αTubulin (Santa Cruz, Dallas, TX, USA) followed by incubation with horseradish peroxidase-conjugated secondary antibodies and enhanced chemoluminescence detection with ChemiDoc (Bio-Rad, Hercules, CA, USA). .. Measurement of hepatic hydroxyproline content Hydroxyproline assay was performed as described previously  .

    Article Title: TGF-β1 Induces the Dual Regulation of Hepatic Progenitor Cells with Both Anti- and Proliver Fibrosis
    Article Snippet: .. The blots were blocked using 5% nonfat dry milk in tris-buffered saline containing Tween 20 for 2 h at room temperature followed by incubation with the primary antibodies against albumin (ALB, diluted 1 : 500, Abcam, Cambridge, UK), α -fetoprotein (AFP, diluted 1 : 1000, R & D Systems, USA), α -smooth muscle actin (SMA, diluted 1 : 1000, R & D Systems, USA), TIMP-1 (diluted 1 : 1000, R & D Systems, USA), and PCNA (diluted 1 : 1000, Abcam, Cambridge, UK) at 4°C overnight. .. After washing, the blots were incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (ZSGB Bio, China) for 1 h at room temperature, and reactivity was detected by the Enhanced Chemiluminescence Kit (Thermo Fisher Scientific Inc., USA).

    Staining:

    Article Title: Tumor Necrosis Factor-? Promotes Cholestasis-Induced Liver Fibrosis in the Mouse through Tissue Inhibitor of Metalloproteinase-1 Production in Hepatic Stellate Cells
    Article Snippet: .. F4/80, CD3, Ki67, TIMP-1, and desmin were stained with anti-F4/80 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-52664), CD3 (Abcam, Cambridge, MA, USA, ab16669), Ki67 (Thermo scientific, RM-9106), TIMP-1 (R & D Systems, Minneapolis, MN, USA), desmin (Lab Vision, Fremont, CA, USA) antibodies using the Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    R&D Systems timp 1
    Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and <t>TIMP-1</t> ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p
    Timp 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/timp 1/product/R&D Systems
    Average 94 stars, based on 356 article reviews
    Price from $9.99 to $1999.99
    timp 1 - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and TIMP-1 ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p

    Journal: Scientific Reports

    Article Title: Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice

    doi: 10.1038/s41598-018-28261-9

    Figure Lengend Snippet: Cytokines, lung function and tissue remodeling after ozone exposure Total cell count in BAL ( A ), epithelial cell desquamation ( B ) and protein measurement of MMP-9 and TIMP-1 ( C ). Lung function measurement ( D ) and tissue remodeling: epithelial damage and infiltration score ( E ) with representative histological images of analyzed small bronchi ( F ). Data were pooled from 3 independent experiments with 5–6 mice per group. Comparison of the ozone-exposed groups with air group. *p

    Article Snippet: TIMP-1 and MMP-9 in BAL supernatant were determined by ELISA (R & D systems, Abingdon , UK ) following the manufacturer’s instructions.

    Techniques: Cell Counting, Mouse Assay

    The effect of collagen peptides alone and in combination with all other bioactives on MMP-1, MMP-3 and TIMP-1 levels and elastin breakdown in NHDF cultures. MMP-1, MMP-3, TIMP-1 and desmosine were measured in supernatants of NHDF grown in 24 well plates and incubated in media plus collagen peptides alone (C) and in combination with the other constituents listed in Table 1 (All) of ACTIVE ( a,c,e,g ) or FORTE ( b,d,f,h ) for 48 hours. Data was expressed as % of media control, normalised to 100% in each experiment and is presented as mean ± SEM of 3 independent experiments. * /† Indicates P

    Journal: Scientific Reports

    Article Title: Effects of collagen-derived bioactive peptides and natural antioxidant compounds on proliferation and matrix protein synthesis by cultured normal human dermal fibroblasts

    doi: 10.1038/s41598-018-28492-w

    Figure Lengend Snippet: The effect of collagen peptides alone and in combination with all other bioactives on MMP-1, MMP-3 and TIMP-1 levels and elastin breakdown in NHDF cultures. MMP-1, MMP-3, TIMP-1 and desmosine were measured in supernatants of NHDF grown in 24 well plates and incubated in media plus collagen peptides alone (C) and in combination with the other constituents listed in Table 1 (All) of ACTIVE ( a,c,e,g ) or FORTE ( b,d,f,h ) for 48 hours. Data was expressed as % of media control, normalised to 100% in each experiment and is presented as mean ± SEM of 3 independent experiments. * /† Indicates P

    Article Snippet: Total TGF-β, total MMP-1, total MMP-3, TIMP-1 and PAI-1 ELISA kits from R & D systems (Abingdon, UK) were used according to the manufacturer’s instructions.

    Techniques: Incubation

    TIMP-1 and TIMP-2 levels in CSF of SLOS patients. Concentrations of (a) TIMP-1 (n=9) and (b) TIMP-2 (n=12) were assessed by ELISA. Both were significantly elevated in SLOS CSF compared to age- and gender-matched controls. (c) TIMP-1 and TIMP-2 levels positively correlated with MMP-2 levels in CSF of SLOS patients. Error bars represent +/− S.D. from the mean.

    Journal: American journal of medical genetics. Part A

    Article Title: Altered Cerebrospinal Fluid Proteins in Smith-Lemli-Opitz Syndrome Patients

    doi: 10.1002/ajmg.a.37720

    Figure Lengend Snippet: TIMP-1 and TIMP-2 levels in CSF of SLOS patients. Concentrations of (a) TIMP-1 (n=9) and (b) TIMP-2 (n=12) were assessed by ELISA. Both were significantly elevated in SLOS CSF compared to age- and gender-matched controls. (c) TIMP-1 and TIMP-2 levels positively correlated with MMP-2 levels in CSF of SLOS patients. Error bars represent +/− S.D. from the mean.

    Article Snippet: Concentrations of MMP-2, TIMP-1, TIMP-2, and MCP-1 in CSF were measured using ELISA kits from R & D Systems according to the manufacturer’s instructions (Product #DMP2F0, #DTM100, #DTM200, #DCP00, respectively).

    Techniques: Enzyme-linked Immunosorbent Assay

    The effect of preincubation of HSCs with 10 μM butein on parameters related to extracellular matrix remodeling induced by ethanol. Western blot analyses for tissue inhibitor of metalloproteinase-1 ( TIMP-1 ) and TIMP-2 were performed on cell lysates derived from cells preincubated for 24 h with 10 μM butein and subsequently incubated for 24 h with the indicated ethanol and acetaldehyde concentrations. The upper panels show representative blots from three independent experiments each with four separate cell cultures, the middle panels show densitometry analysis of bands, and the lower panels show the TIMP-1 ELISA assay. *Significantly different from respective controls ( C cells not treated, C-butein treated only with butein), p ≤ 0.01. # Statistically significant in comparison to cells treated with ethanol or acetaldehyde alone, # p ≤ 0.05, ## p ≤ 0.001. Butein significantly changed the ethanol ( p ≤ 0.01) and acetaldehyde ( p ≤ 0.1) effect (two-way ANOVA)

    Journal: Journal of Gastroenterology

    Article Title: Butein inhibits ethanol-induced activation of liver stellate cells through TGF-?, NF?B, p38, and JNK signaling pathways and inhibition of oxidative stress

    doi: 10.1007/s00535-012-0619-7

    Figure Lengend Snippet: The effect of preincubation of HSCs with 10 μM butein on parameters related to extracellular matrix remodeling induced by ethanol. Western blot analyses for tissue inhibitor of metalloproteinase-1 ( TIMP-1 ) and TIMP-2 were performed on cell lysates derived from cells preincubated for 24 h with 10 μM butein and subsequently incubated for 24 h with the indicated ethanol and acetaldehyde concentrations. The upper panels show representative blots from three independent experiments each with four separate cell cultures, the middle panels show densitometry analysis of bands, and the lower panels show the TIMP-1 ELISA assay. *Significantly different from respective controls ( C cells not treated, C-butein treated only with butein), p ≤ 0.01. # Statistically significant in comparison to cells treated with ethanol or acetaldehyde alone, # p ≤ 0.05, ## p ≤ 0.001. Butein significantly changed the ethanol ( p ≤ 0.01) and acetaldehyde ( p ≤ 0.1) effect (two-way ANOVA)

    Article Snippet: Additionally the cell culture supernatants were centrifuged and frozen immediately at −80 °C for further cytokine (TGF-β), MMP-2, and TIMP-1 level measurements, using a sandwich enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions; ELISA kits for the detection of rat proteins were purchased from R & D Systems.

    Techniques: Western Blot, Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay