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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of <t>normal</t> <t>THLE-2</t> cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).
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In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of normal THLE-2 cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).

Journal: International Journal of Nanomedicine

Article Title: Sequential-Delivery Gel Microspheres Integrating HAase-Mediated ECM Remodeling and GA-Active Targeting for Deep Tumor Penetration in TACE-Treated HCC

doi: 10.2147/IJN.S634549

Figure Lengend Snippet: In vitro assessment of HAase/BUF NPs-GM. ( A ) Flow cytometry analysis of Huh-7 cell uptake after incubation with the indicated formulations for 2, 6, 12, 24, 48, and 72 h. ( B ) Quantification of mean fluorescence intensity from ( A ). ( C ) Cell viability of Huh-7 cells treated with free BUF, BUF NPs, GA single load, GA dual load, No GA dual load, or No GA and HYD dual load at BUF concentrations of 0–200 nM for 72 h. ( D ) IC 5 0 values derived by nonlinear fitting. ( E ) Viability of normal THLE-2 cells treated with BUF NPs, GA single load, or GA dual load at 104.97 nM BUF for 72 h. Data are mean ± SD from three independent experiments (n = 3). **p < 0.01, *p < 0.05 vs the indicated group (one-way ANOVA with LSD post-hoc test, SPSS version 19).

Article Snippet: Huh-7 hepatocellular carcinoma cells (BNCC337690) and normal THLE-2 liver cells (CRL-2638) were purchased from BeNa Culture Collection and ATCC, respectively.

Techniques: In Vitro Evaluation, Flow Cytometry, Analysis, Incubation, Fluorescence, Derivative Assay