thioglycolate broth  (Millipore)


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    Name:
    Thioglycolate Broth
    Description:

    Catalog Number:
    90404
    Price:
    None
    Applications:
    For sterility testing of biologicals and for cultivation of aerobic and anaerobic organisms. Any increase in the oxygen content is indicated by a color change of redox indicator resazurin to red.
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    Structured Review

    Millipore thioglycolate broth
    Peritoneal macrophage phagocytosis in mice fed a calorie-restricted diet. Six-month-old C57BL/6 mice were fed a semipurified diet containing 5% (wt/wt) corn oil AL or the same diet whose calories were restricted 40% (CR). Peritoneal macrophages were harvested 96 h after injection of <t>thioglycolate</t> intraperitoneally. Phagocytosis of latex beads and zymosan was assessed before and 4 h after LPS stimulation (1 μg/ml) ( n = 6). ∗, significant differences between the group fed AL and the group fed a calorie-restricted diet ( P

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    Images

    1) Product Images from "Effects of Calorie Restriction on Polymicrobial Peritonitis Induced by Cecum Ligation and Puncture in Young C57BL/6 Mice"

    Article Title: Effects of Calorie Restriction on Polymicrobial Peritonitis Induced by Cecum Ligation and Puncture in Young C57BL/6 Mice

    Journal: Clinical and Diagnostic Laboratory Immunology

    doi: 10.1128/CDLI.8.5.1003-1011.2001

    Peritoneal macrophage phagocytosis in mice fed a calorie-restricted diet. Six-month-old C57BL/6 mice were fed a semipurified diet containing 5% (wt/wt) corn oil AL or the same diet whose calories were restricted 40% (CR). Peritoneal macrophages were harvested 96 h after injection of thioglycolate intraperitoneally. Phagocytosis of latex beads and zymosan was assessed before and 4 h after LPS stimulation (1 μg/ml) ( n = 6). ∗, significant differences between the group fed AL and the group fed a calorie-restricted diet ( P
    Figure Legend Snippet: Peritoneal macrophage phagocytosis in mice fed a calorie-restricted diet. Six-month-old C57BL/6 mice were fed a semipurified diet containing 5% (wt/wt) corn oil AL or the same diet whose calories were restricted 40% (CR). Peritoneal macrophages were harvested 96 h after injection of thioglycolate intraperitoneally. Phagocytosis of latex beads and zymosan was assessed before and 4 h after LPS stimulation (1 μg/ml) ( n = 6). ∗, significant differences between the group fed AL and the group fed a calorie-restricted diet ( P

    Techniques Used: Mouse Assay, Injection

    2) Product Images from "LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBPε in granulocytes"

    Article Title: LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBPε in granulocytes

    Journal: Scientific Reports

    doi: 10.1038/srep46440

    Trem1 expression in BM and PEC. ( A ) Surface expression of Trem1 on Gr-1hi granulocytes from the BM was analyzed by flow cytometry using antibodies against murine Trem1, Gr-1, and Mac-1. Contour plots and histograms show a representative staining in WT and C/EBPε KO mice. Histograms indicate Trem1 expression compared to negative control (IgG antibody; dotted line/open histogram) in Gr-1hi granulocytes (solid line/tinted histogram) in WT (upper panel) and C/EBPε KO (lower panel) from the BM. The bar graph shows a summary of Trem1 expression (%) on Gr-1hi BM cells of WT and C/EBPε KO mice (n = 6). ( B ) Number of PECs in the WT and C/EBPε KO mice were counted at 4 hours (1.1 ± 0.3 vs 0.16 ± 0.03 × 10 7 cells, WT vs KO respectively, n = 6, p = 0.002) and 18 hours (1.6 ± 0.14 vs 1.3 ± 0.07 × 10 7 cells, respectively, n = 6, p = 0.33) after intraperitoneal thioglycolate injection, * p
    Figure Legend Snippet: Trem1 expression in BM and PEC. ( A ) Surface expression of Trem1 on Gr-1hi granulocytes from the BM was analyzed by flow cytometry using antibodies against murine Trem1, Gr-1, and Mac-1. Contour plots and histograms show a representative staining in WT and C/EBPε KO mice. Histograms indicate Trem1 expression compared to negative control (IgG antibody; dotted line/open histogram) in Gr-1hi granulocytes (solid line/tinted histogram) in WT (upper panel) and C/EBPε KO (lower panel) from the BM. The bar graph shows a summary of Trem1 expression (%) on Gr-1hi BM cells of WT and C/EBPε KO mice (n = 6). ( B ) Number of PECs in the WT and C/EBPε KO mice were counted at 4 hours (1.1 ± 0.3 vs 0.16 ± 0.03 × 10 7 cells, WT vs KO respectively, n = 6, p = 0.002) and 18 hours (1.6 ± 0.14 vs 1.3 ± 0.07 × 10 7 cells, respectively, n = 6, p = 0.33) after intraperitoneal thioglycolate injection, * p

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Staining, Mouse Assay, Negative Control, Injection

    3) Product Images from "Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells"

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells

    Journal: Annals of neurology

    doi: 10.1002/ana.24020

    Macrophage responses to TLR stimulation in p38CKO Lysm mice Female and male WT and p38CKO Lysm mice were immunized using the 2×MOG 35-55 /CFA protocol. On day 6 thioglycolate was injected i.p., and elicited peritoneal macrophages were isolated on day 10 post-immunization. Adherent macrophages were stimulated for 30 min with 100 ng/ml LPS ( A ) or 50 μg/ml heat killed MTB ( B ), lysed, and analyzed by immunoblot for phosphorylation of p38 MAPK (p-p38). GAPDH is shown as a loading control. Panel ( B ) is a composite image of two different parts of the same membrane image, as indicated by the lines, treated identically and shown at the same exposure. Alternatively, adherent macrophages were stimulated for 4 or 24 hrs (as indicated) with LPS ( C and D ) or MTB ( E and F ), supernatants were collected and analyzed by ELISA for the presence of TNFα or IL-6. Data are representative of 3 independent experiments. * ≤ 0.05; ** ≤ 0.01. WT female (n=6), p38CKO Lysm female (n=9), WT male (n=8), p38CKO Lysm male (n=8).
    Figure Legend Snippet: Macrophage responses to TLR stimulation in p38CKO Lysm mice Female and male WT and p38CKO Lysm mice were immunized using the 2×MOG 35-55 /CFA protocol. On day 6 thioglycolate was injected i.p., and elicited peritoneal macrophages were isolated on day 10 post-immunization. Adherent macrophages were stimulated for 30 min with 100 ng/ml LPS ( A ) or 50 μg/ml heat killed MTB ( B ), lysed, and analyzed by immunoblot for phosphorylation of p38 MAPK (p-p38). GAPDH is shown as a loading control. Panel ( B ) is a composite image of two different parts of the same membrane image, as indicated by the lines, treated identically and shown at the same exposure. Alternatively, adherent macrophages were stimulated for 4 or 24 hrs (as indicated) with LPS ( C and D ) or MTB ( E and F ), supernatants were collected and analyzed by ELISA for the presence of TNFα or IL-6. Data are representative of 3 independent experiments. * ≤ 0.05; ** ≤ 0.01. WT female (n=6), p38CKO Lysm female (n=9), WT male (n=8), p38CKO Lysm male (n=8).

    Techniques Used: Mouse Assay, Injection, Isolation, Enzyme-linked Immunosorbent Assay

    4) Product Images from "The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines"

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.052702999

    Production of NO from mouse peritoneal macrophages. Thioglycolate-elicited peritoneal macrophages were incubated in vitro with increasing concentrations of SAHA for 1 h and then stimulated with the combination of human TNF-α (10 ng/ml) plus murine IFN-γ (100 units/ml). After 24 h, the supernatants were assayed for NO. Mean ± SE of four separate experiments. *, P
    Figure Legend Snippet: Production of NO from mouse peritoneal macrophages. Thioglycolate-elicited peritoneal macrophages were incubated in vitro with increasing concentrations of SAHA for 1 h and then stimulated with the combination of human TNF-α (10 ng/ml) plus murine IFN-γ (100 units/ml). After 24 h, the supernatants were assayed for NO. Mean ± SE of four separate experiments. *, P

    Techniques Used: Incubation, In Vitro

    Related Articles

    Centrifugation:

    Article Title: Cytadherence of Mycoplasma pneumoniae Induces Inflammatory Responses through Autophagy and Toll-Like Receptor 4
    Article Snippet: Thioglycolate broth (2 ml) (Sigma-Aldrich, St. Louis, MO) was injected into the peritoneal cavities of C57BL, TLR2 KO, TLR4 KO, and TLR2/4 double-KO mice. .. After 72 h later, peritoneal exudate macrophages were harvested by centrifugation.

    Recombinant:

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines
    Article Snippet: Lipopolysaccharide (LPS, Escherichia coli 055:B5), Griess reagent, ficoll, polyvinylpyrolidone, dextran sulfate, trypsin, thioglycolate broth, Con A, and salmon sperm DNA were from Sigma (or Sigma, Milan). .. Human recombinant IL-18 was purchased from R & D Systems, and recombinant human IL-12, IFN-γ, TNF-α, IL-8, and IL-1-β as well as recombinant murine IFN-γ were from PeproTech (Rocky Hill, NJ).

    Proton NMR:

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines
    Article Snippet: After synthesis, the structure of SAHA was confirmed by mass spectrometry and proton NMR spectroscopy. .. Lipopolysaccharide (LPS, Escherichia coli 055:B5), Griess reagent, ficoll, polyvinylpyrolidone, dextran sulfate, trypsin, thioglycolate broth, Con A, and salmon sperm DNA were from Sigma (or Sigma, Milan).

    Expressing:

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Expression plasmids for LacZ, PAK1, and the kinase-dead mutant of PAK1 were described previously ( ). .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich).

    Mutagenesis:

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Expression plasmids for LacZ, PAK1, and the kinase-dead mutant of PAK1 were described previously ( ). .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich).

    Article Title: Characterization of Clostridium perfringens TpeL Toxin Gene Carriage, Production, Cytotoxic Contributions, and Trypsin Sensitivity
    Article Snippet: FTG medium (fluid thioglycolate medium; Difco Laboratories), TH medium (Bacto Todd Hewitt broth [Becton-Dickinson], with 0.1% sodium thioglycolate [Sigma-Aldrich]), TGY medium (3% tryptic soy broth [Becton-Dickinson] containing 2% glucose [Fisher Scientific], 1% yeast extract [Becton-Dickinson], and 0.1% thioglycolate [Sigma-Aldrich]), or TY medium (3% tryptic soy broth [Becton-Dickinson], 1% yeast extract [Becton-Dickinson], and 0.1% thioglycolate [Sigma-Aldrich]) was used to grow broth cultures of C. perfringens . .. E. coli strain TOP 10 was grown in Luria-Bertani (LB) broth and used for constructing toxin gene mutagenesis plasmids.

    Isolation:

    Article Title: Effects of Calorie Restriction on Polymicrobial Peritonitis Induced by Cecum Ligation and Puncture in Young C57BL/6 Mice
    Article Snippet: Paragraph title: Peritoneal macrophage isolation and culture. ... Ninety-six hours before the mice were killed, the mice received an intraperitoneal injection of 2.0 ml of thioglycolate broth (Sigma Chemical Co.).

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells
    Article Snippet: Paragraph title: Isolation and stimulation of thioglycolate-elicited macrophages ... On day 6 post-immunization, mice were injected with 1ml of a 4% solution of thioglycolate broth (Sigma-Adrich, USA) i.p. 96 hours later mice were sacrificed and the peritoneal cavity was flushed with 15 ml of cold PBS.

    Article Title: Cytadherence of Mycoplasma pneumoniae Induces Inflammatory Responses through Autophagy and Toll-Like Receptor 4
    Article Snippet: Paragraph title: Isolation of mouse peritoneal macrophages. ... Thioglycolate broth (2 ml) (Sigma-Aldrich, St. Louis, MO) was injected into the peritoneal cavities of C57BL, TLR2 KO, TLR4 KO, and TLR2/4 double-KO mice.

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich). .. Generally, 90% of the cells isolated after 72 hours were F4/80 positive, and more than 60% were F4/80 positive after 24-hour induction.

    Article Title: Sinomenine Hydrochloride Protects against Polymicrobial Sepsis via Autophagy
    Article Snippet: Paragraph title: 4.7. Isolation of Peritoneal Macrophages and Cell Treatments ... Mice were injected intraperitoneal injection (i.p.) with 3 mL thioglycolate broth (Sigma-Aldrich); and three days later, PMs were recovered by peritoneal lavage with Dulbecco phosphate-buffered saline.

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: Paragraph title: Isolation and culture of mouse peritoneal macrophages, bone marrow-derived macrophages and lung endothelial cells ... For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate.

    Article Title: LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBPε in granulocytes
    Article Snippet: Paragraph title: Isolation of mouse peritoneal granulocytes ... Briefly, 2 ml of 4% thioglycolate broth (Sigma Chemical Co., St. Louis, MO) was intraperitoneally injected.

    Nick Translation:

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines
    Article Snippet: Lipopolysaccharide (LPS, Escherichia coli 055:B5), Griess reagent, ficoll, polyvinylpyrolidone, dextran sulfate, trypsin, thioglycolate broth, Con A, and salmon sperm DNA were from Sigma (or Sigma, Milan). .. Guanidine isothiocyanate, SDS, formamide, and 2-mercaptoethanol were purchased Bio-Rad, 3 H-thymidine and [α-32 P]dCTP from Amersham Pharmacia and nick translation kit from Promega.

    Transfection:

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Raw264.7 cells were transfected using Lipofectamine (Invitrogen). .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich).

    Cell Culture:

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells
    Article Snippet: On day 6 post-immunization, mice were injected with 1ml of a 4% solution of thioglycolate broth (Sigma-Adrich, USA) i.p. 96 hours later mice were sacrificed and the peritoneal cavity was flushed with 15 ml of cold PBS. .. Cells were washed and cultured overnight in RPMI + 5% FBS, then washed to remove non-adherent cells.

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Raw264.7 cells were cultured in DMEM (Cellgro) supplemented with 10% FCS (Hyclone; heat-inactivated), penicillin and streptomycin. .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich).

    Article Title: Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement *
    Article Snippet: Cells Thioglycolate-elicited Mφ (thio-Mφ) were obtained by intraperitoneal injection of 4% (w/v) brewers' thioglycolate broth (Sigma) 4 days before harvest. .. Mφ were cultured overnight on 6-well tissue culture plates (1.25 × 106 cells/well; BD Biosciences) in RPMI 1640 medium (Sigma) supplemented with R-10 medium (10% heat-inactivated (56 °C at 30 min) FBS (Sigma), 2 mm glutamine (Sigma), 100 units/ml penicillin, 100 μg/ml streptomycin (Sigma), and 10 mm Hepes (Invitrogen)) and washed three times with PBS prior use.

    Article Title: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage
    Article Snippet: Cell isolation and stimulation Mouse bone marrow cells were collected from femurs and tibias by flushing with Dulbecco’s modified Eagle medium (DMEM; Gibco) containing 10% FBS through a 25-G needle and cultured in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 10 ng/mL rmM-CSF (PeproTech, Rocky Hill, NJ, USA) for 5 days to generate BMDMs. .. PMs were obtained by intraperitoneal injection of 2 mL sterile 3% thioglycolate broth (Millipore, Billerica, MA, USA).

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate. .. For bone marrow-derived M1 or M2 macrophages, bone marrow cells were harvested from the femur and tibia of male Rap1+/+ and Rap1−/− mice (8 weeks old) and cultured in RPMI1640 medium supplemented with 10% FBS, 0.05 mM 2-mercaptoethanol and 1% penicillin/streptomycin (100 U/ml).

    Mouse Assay:

    Article Title: Effects of Calorie Restriction on Polymicrobial Peritonitis Induced by Cecum Ligation and Puncture in Young C57BL/6 Mice
    Article Snippet: .. Ninety-six hours before the mice were killed, the mice received an intraperitoneal injection of 2.0 ml of thioglycolate broth (Sigma Chemical Co.). .. The mice were killed by carbon dioxide inhalation.

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells
    Article Snippet: .. On day 6 post-immunization, mice were injected with 1ml of a 4% solution of thioglycolate broth (Sigma-Adrich, USA) i.p. 96 hours later mice were sacrificed and the peritoneal cavity was flushed with 15 ml of cold PBS. .. Cells were washed and cultured overnight in RPMI + 5% FBS, then washed to remove non-adherent cells.

    Article Title: Cytadherence of Mycoplasma pneumoniae Induces Inflammatory Responses through Autophagy and Toll-Like Receptor 4
    Article Snippet: .. Thioglycolate broth (2 ml) (Sigma-Aldrich, St. Louis, MO) was injected into the peritoneal cavities of C57BL, TLR2 KO, TLR4 KO, and TLR2/4 double-KO mice. .. After 72 h later, peritoneal exudate macrophages were harvested by centrifugation.

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich). .. Peritoneal cavities were lavaged with 10 ml HBSS (Gibco; Invitrogen), and an aliquot of cells was stained with the anti-F4/80 antibody.

    Article Title: Sinomenine Hydrochloride Protects against Polymicrobial Sepsis via Autophagy
    Article Snippet: .. Mice were injected intraperitoneal injection (i.p.) with 3 mL thioglycolate broth (Sigma-Aldrich); and three days later, PMs were recovered by peritoneal lavage with Dulbecco phosphate-buffered saline. ..

    Article Title: Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement *
    Article Snippet: Cells Thioglycolate-elicited Mφ (thio-Mφ) were obtained by intraperitoneal injection of 4% (w/v) brewers' thioglycolate broth (Sigma) 4 days before harvest. .. Mice were killed, and the peritoneal cavities were rinsed with PBS (Sigma) containing 5 mm EDTA.

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: .. For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate. .. Cells (3×10 /ml/well) resuspended in Dulbecco's Modified Eagle medium (Cat. no. 11965–092, Invitrogen) supplemented with 10% FBS and 1% penicillin/streptomycin (100 U/ml) were seeded onto 24-wells plate.

    Spectroscopy:

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines
    Article Snippet: After synthesis, the structure of SAHA was confirmed by mass spectrometry and proton NMR spectroscopy. .. Lipopolysaccharide (LPS, Escherichia coli 055:B5), Griess reagent, ficoll, polyvinylpyrolidone, dextran sulfate, trypsin, thioglycolate broth, Con A, and salmon sperm DNA were from Sigma (or Sigma, Milan).

    Incubation:

    Article Title: Investigation of adherent-invasive E. coli in patients with Crohn's disease
    Article Snippet: Microbial identification Samples were immediately transferred into sterile vials containing either thioglycolate broth or saline (Sigma-Aldrich, Hi Media) and stored at -20˚C. .. The tissue biopsy were homogenized and inoculated into Hi Chrome E. coli agar (Sigma-Aldrich, Hi Media) and incubated for 18-24h at 35±2°C.

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate. .. After four hours of incubation at 37°C, adhered macrophages were treated with LPS (5ng/ml, 4 hours) before harvesting.

    Mass Spectrometry:

    Article Title: The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines
    Article Snippet: After synthesis, the structure of SAHA was confirmed by mass spectrometry and proton NMR spectroscopy. .. Lipopolysaccharide (LPS, Escherichia coli 055:B5), Griess reagent, ficoll, polyvinylpyrolidone, dextran sulfate, trypsin, thioglycolate broth, Con A, and salmon sperm DNA were from Sigma (or Sigma, Milan).

    Western Blot:

    Article Title: In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system, et al. In vitro assessment of quality of citrate‐phosphate‐dextrose‐adenine‐1 preserved feline blood collected by a commercial closed system
    Article Snippet: Paragraph title: 2.2. WB CPDA‐1 analysis ... In addition, 1 mL of blood was added to 9 mL of nutrient broth (Heart Infusion broth Oxoid, Basingstoke, UK) and Thioglycolate broth (THG broth, Sigma Aldrich, Milan, Italy).

    Modification:

    Article Title: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage
    Article Snippet: Cell isolation and stimulation Mouse bone marrow cells were collected from femurs and tibias by flushing with Dulbecco’s modified Eagle medium (DMEM; Gibco) containing 10% FBS through a 25-G needle and cultured in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 10 ng/mL rmM-CSF (PeproTech, Rocky Hill, NJ, USA) for 5 days to generate BMDMs. .. PMs were obtained by intraperitoneal injection of 2 mL sterile 3% thioglycolate broth (Millipore, Billerica, MA, USA).

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate. .. Cells (3×10 /ml/well) resuspended in Dulbecco's Modified Eagle medium (Cat. no. 11965–092, Invitrogen) supplemented with 10% FBS and 1% penicillin/streptomycin (100 U/ml) were seeded onto 24-wells plate.

    Staining:

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich). .. Peritoneal cavities were lavaged with 10 ml HBSS (Gibco; Invitrogen), and an aliquot of cells was stained with the anti-F4/80 antibody.

    Injection:

    Article Title: Effects of Calorie Restriction on Polymicrobial Peritonitis Induced by Cecum Ligation and Puncture in Young C57BL/6 Mice
    Article Snippet: .. Ninety-six hours before the mice were killed, the mice received an intraperitoneal injection of 2.0 ml of thioglycolate broth (Sigma Chemical Co.). .. The mice were killed by carbon dioxide inhalation.

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells
    Article Snippet: .. On day 6 post-immunization, mice were injected with 1ml of a 4% solution of thioglycolate broth (Sigma-Adrich, USA) i.p. 96 hours later mice were sacrificed and the peritoneal cavity was flushed with 15 ml of cold PBS. .. Cells were washed and cultured overnight in RPMI + 5% FBS, then washed to remove non-adherent cells.

    Article Title: Cytadherence of Mycoplasma pneumoniae Induces Inflammatory Responses through Autophagy and Toll-Like Receptor 4
    Article Snippet: .. Thioglycolate broth (2 ml) (Sigma-Aldrich, St. Louis, MO) was injected into the peritoneal cavities of C57BL, TLR2 KO, TLR4 KO, and TLR2/4 double-KO mice. .. After 72 h later, peritoneal exudate macrophages were harvested by centrifugation.

    Article Title: Phospholipase C ?3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
    Article Snippet: .. Peritoneal macrophages were prepared 24 hours or 72 hours after mice were injected with 1 ml of 3% thioglycolate broth (Sigma-Aldrich). .. Peritoneal cavities were lavaged with 10 ml HBSS (Gibco; Invitrogen), and an aliquot of cells was stained with the anti-F4/80 antibody.

    Article Title: Sinomenine Hydrochloride Protects against Polymicrobial Sepsis via Autophagy
    Article Snippet: .. Mice were injected intraperitoneal injection (i.p.) with 3 mL thioglycolate broth (Sigma-Aldrich); and three days later, PMs were recovered by peritoneal lavage with Dulbecco phosphate-buffered saline. ..

    Article Title: Fungal Recognition Enhances Mannose Receptor Shedding through Dectin-1 Engagement *
    Article Snippet: .. Cells Thioglycolate-elicited Mφ (thio-Mφ) were obtained by intraperitoneal injection of 4% (w/v) brewers' thioglycolate broth (Sigma) 4 days before harvest. .. Mice were killed, and the peritoneal cavities were rinsed with PBS (Sigma) containing 5 mm EDTA.

    Article Title: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage
    Article Snippet: .. PMs were obtained by intraperitoneal injection of 2 mL sterile 3% thioglycolate broth (Millipore, Billerica, MA, USA). .. 3 days after injection, cells were harvested by peritoneal lavage with 5 mL cold PBS.

    Article Title: Rap1 induces cytokine production in pro-inflammatory macrophages through NFκB signaling and is highly expressed in human atherosclerotic lesions
    Article Snippet: .. For peritoneal macrophages, 8 weeks old male Rap1+/+ and Rap1−/− mice were injected with 2ml of 4% thioglycolate broth (Cat. no. 90404, Sigma) into their peritoneal cavity 3 d prior to collection of the peritoneal exudate. .. Cells (3×10 /ml/well) resuspended in Dulbecco's Modified Eagle medium (Cat. no. 11965–092, Invitrogen) supplemented with 10% FBS and 1% penicillin/streptomycin (100 U/ml) were seeded onto 24-wells plate.

    Article Title: LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBPε in granulocytes
    Article Snippet: .. Briefly, 2 ml of 4% thioglycolate broth (Sigma Chemical Co., St. Louis, MO) was intraperitoneally injected. .. After 4 and 18 hours of injection, the peritoneal lavage was collected with cold phosphate buffered saline containing 0.2% albumin.

    Purification:

    Article Title: Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells
    Article Snippet: On day 6 post-immunization, mice were injected with 1ml of a 4% solution of thioglycolate broth (Sigma-Adrich, USA) i.p. 96 hours later mice were sacrificed and the peritoneal cavity was flushed with 15 ml of cold PBS. .. The remaining adherent cells were stimulated with purified LPS (Sigma) or heat-killed Mycobacterium tuberculosis H37Ra (Difco, USA).

    Article Title: Sinomenine Hydrochloride Protects against Polymicrobial Sepsis via Autophagy
    Article Snippet: Isolation of Peritoneal Macrophages and Cell Treatments The murine PMs were isolated and purified as described previously [ ]. .. Mice were injected intraperitoneal injection (i.p.) with 3 mL thioglycolate broth (Sigma-Aldrich); and three days later, PMs were recovered by peritoneal lavage with Dulbecco phosphate-buffered saline.

    Cell Isolation:

    Article Title: Regulation and biological role of the peptide/histidine transporter SLC15A3 in Toll-like receptor-mediated inflammatory responses in macrophage
    Article Snippet: Paragraph title: Cell isolation and stimulation ... PMs were obtained by intraperitoneal injection of 2 mL sterile 3% thioglycolate broth (Millipore, Billerica, MA, USA).

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    Millipore thioglycolate broth
    Thioglycolate Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thioglycolate broth/product/Millipore
    Average 99 stars, based on 1 article reviews
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    thioglycolate broth - by Bioz Stars, 2020-04
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