thermostart taq polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher thermostart taq polymerase
    Thermostart Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermostart taq polymerase/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    thermostart taq polymerase - by Bioz Stars, 2020-05
    85/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Dissecting Epigenetic Silencing Complexity in the Mouse Lung Cancer Suppressor Gene Cadm1
    Article Snippet: .. Semi-quantitative RT-PCR was performed using Thermostart Taq polymerase (ABgene) on T3 thermocyclers (Biometra), whereas quantitative RT-PCR on Light Cycler (Roche) using Absolute qPCR Sybr Green Capillary (ThermoFisher). ..

    Multiplex Assay:

    Article Title: Spatial and temporal genetic structure of a river-resident Atlantic salmon (Salmo salar) after millennia of isolation
    Article Snippet: .. The multiplex reactions were carried out in a total volume of 10 μ L, containing 11 μ mol/L and 6.5 μ mol/L of total primers, in multiplex 1 and 2, respectively, but with different concentrations for each pair, 1 mmol/L of total dNTP, 1X reaction buffer, 2.25 mmol/L MgCl2 , and 0.75 units of Thermostart taq polymerase (Thermo Scientific Inc., Waltham, MA). .. The following PCR program was run on a Quattro Cycler (VWR): denaturation for 15 min at 95°C: six cycles touchdown PCR of denaturation at 94°C, annealing temperature from 58°C to 52 °C, and extension at 72°C for 60 s. The last 24 cycles were run with denaturation at 94°C for 30 s, annealing at 52°C for 30 s, and extension at 72°C for 60 s; a final step of extension at 72°C for 10 min. Fragments from each multiplex were separated and visualized separately on an ABI 3130xl DNA analyser (Applied Biosystems) and sizing using genemapper ver.

    Amplification:

    Article Title: Identification of the Main Promoter Directing Cereulide Biosynthesis in Emetic Bacillus cereus and Its Application for Real-Time Monitoring of ces Gene Expression in Foods ▿ Gene Expression in Foods ▿ †
    Article Snippet: .. The 50-μl PCR mixture (10 ng DNA, 0.5 μM each primer, 1.5 mM MgCl2 , 0.4 mM each deoxynucleoside triphosphate [dNTP], 1.25 U ThermoStart Taq polymerase [all from ABgene]) was activated (95°C for 15 min), followed by 30 amplification cycles (95°C for 30 s, 60°C for 45 s, and 72°C for 1 min) and an elongation step (72°C for 5 min). .. Total DNA was isolated as described previously , and plasmid DNA was prepared using standard procedures.

    Article Title: PTCH mutations in basal cell carcinomas from azathioprine-treated organ transplant recipients
    Article Snippet: .. PCRs for MCA contained PCR buffer, 200 μ M dNTPs, MgCl2 as required ( online), 1 μ l LCGreen+ (all Idaho Technologies), 0.25 U of ThermoStart Taq polymerase (ABgene, Epsom, UK), primers as required, DMSO to a final concentration of 5%, 20 ng of genomic DNA and sufficient water to make a final volume of 10 μ l. Thermal cycling conditions were 95°C for 12 min to activate the Taq polymerase followed by 45 cycles of 94°C for 10 s, annealing temperature ( online) for 15 s and 72°C for 15 s. To ensure maximum incorporation of LCGreen+ into double-stranded amplified DNA, PCR products were heated to 94°C for 30 s and allowed to cool to 25°C at 0.1°C per second. .. PCR primer sequences and amplification conditions for each amplified fragment are shown in online.

    Polymerase Chain Reaction:

    Article Title: Identification of the Main Promoter Directing Cereulide Biosynthesis in Emetic Bacillus cereus and Its Application for Real-Time Monitoring of ces Gene Expression in Foods ▿ Gene Expression in Foods ▿ †
    Article Snippet: .. The 50-μl PCR mixture (10 ng DNA, 0.5 μM each primer, 1.5 mM MgCl2 , 0.4 mM each deoxynucleoside triphosphate [dNTP], 1.25 U ThermoStart Taq polymerase [all from ABgene]) was activated (95°C for 15 min), followed by 30 amplification cycles (95°C for 30 s, 60°C for 45 s, and 72°C for 1 min) and an elongation step (72°C for 5 min). .. Total DNA was isolated as described previously , and plasmid DNA was prepared using standard procedures.

    Article Title: PTCH mutations in basal cell carcinomas from azathioprine-treated organ transplant recipients
    Article Snippet: .. PCRs for MCA contained PCR buffer, 200 μ M dNTPs, MgCl2 as required ( online), 1 μ l LCGreen+ (all Idaho Technologies), 0.25 U of ThermoStart Taq polymerase (ABgene, Epsom, UK), primers as required, DMSO to a final concentration of 5%, 20 ng of genomic DNA and sufficient water to make a final volume of 10 μ l. Thermal cycling conditions were 95°C for 12 min to activate the Taq polymerase followed by 45 cycles of 94°C for 10 s, annealing temperature ( online) for 15 s and 72°C for 15 s. To ensure maximum incorporation of LCGreen+ into double-stranded amplified DNA, PCR products were heated to 94°C for 30 s and allowed to cool to 25°C at 0.1°C per second. .. PCR primer sequences and amplification conditions for each amplified fragment are shown in online.

    Article Title: A t(1;11) translocation linked to schizophrenia and affective disorders gives rise to aberrant chimeric DISC1 transcripts that encode structurally altered, deleterious mitochondrial proteins
    Article Snippet: .. PCR, to detect novel DISC1 chimeric transcripts, was carried out using Thermostart Taq polymerase (Abgene). .. A typical reaction contains 2 μl of 10× PCR reaction buffer, 1.2 μl of 25 mm magnesium chloride, 0.5 μl of dNTPs (of a 10 mm stock), 0.25 μl of forward and reverse primers (of a 300 ng/μl stock), 0.1 μl of Taq polymerase, 2 μl of DNA template and 13.7 μl of sterile water to a final volume of 20 μl.

    Quantitative RT-PCR:

    Article Title: Dissecting Epigenetic Silencing Complexity in the Mouse Lung Cancer Suppressor Gene Cadm1
    Article Snippet: .. Semi-quantitative RT-PCR was performed using Thermostart Taq polymerase (ABgene) on T3 thermocyclers (Biometra), whereas quantitative RT-PCR on Light Cycler (Roche) using Absolute qPCR Sybr Green Capillary (ThermoFisher). ..

    SYBR Green Assay:

    Article Title: Dissecting Epigenetic Silencing Complexity in the Mouse Lung Cancer Suppressor Gene Cadm1
    Article Snippet: .. Semi-quantitative RT-PCR was performed using Thermostart Taq polymerase (ABgene) on T3 thermocyclers (Biometra), whereas quantitative RT-PCR on Light Cycler (Roche) using Absolute qPCR Sybr Green Capillary (ThermoFisher). ..

    Concentration Assay:

    Article Title: PTCH mutations in basal cell carcinomas from azathioprine-treated organ transplant recipients
    Article Snippet: .. PCRs for MCA contained PCR buffer, 200 μ M dNTPs, MgCl2 as required ( online), 1 μ l LCGreen+ (all Idaho Technologies), 0.25 U of ThermoStart Taq polymerase (ABgene, Epsom, UK), primers as required, DMSO to a final concentration of 5%, 20 ng of genomic DNA and sufficient water to make a final volume of 10 μ l. Thermal cycling conditions were 95°C for 12 min to activate the Taq polymerase followed by 45 cycles of 94°C for 10 s, annealing temperature ( online) for 15 s and 72°C for 15 s. To ensure maximum incorporation of LCGreen+ into double-stranded amplified DNA, PCR products were heated to 94°C for 30 s and allowed to cool to 25°C at 0.1°C per second. .. PCR primer sequences and amplification conditions for each amplified fragment are shown in online.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Dissecting Epigenetic Silencing Complexity in the Mouse Lung Cancer Suppressor Gene Cadm1
    Article Snippet: .. Semi-quantitative RT-PCR was performed using Thermostart Taq polymerase (ABgene) on T3 thermocyclers (Biometra), whereas quantitative RT-PCR on Light Cycler (Roche) using Absolute qPCR Sybr Green Capillary (ThermoFisher). ..

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  • 99
    Thermo Fisher thermostart taq dna polymerase
    Thermostart Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermostart taq dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    thermostart taq dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
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