thermo resistant hotstar taq dna polymerase  (Qiagen)


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    Structured Review

    Qiagen thermo resistant hotstar taq dna polymerase
    Thermo Resistant Hotstar Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo resistant hotstar taq dna polymerase/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermo resistant hotstar taq dna polymerase - by Bioz Stars, 2020-04
    90/100 stars

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    30-cycle amplification reactions

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    Related Articles

    Clone Assay:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: Paragraph title: 2.2. Cloning and recombinant expression of the CnMT1 and CnMT2 cDNAs ... The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation).

    Amplification:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: .. The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    Agarose Gel Electrophoresis:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    DNA Ligation:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    Ligation:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    Activation Assay:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: .. The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    Polymerase Chain Reaction:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The two restriction sites were added to the cDNA sequences by PCR amplification, using the following oligonucleotides as primers: 5′-AAAAGGATCCATGGCTTGCAACTGCCCTCCCCAGA-3′ (forward) and 5′-AAAACTCGAGTCAGGCAGCGCCAG-3′ (reverse) for CnMT1; and 5′-GGGAGAATTCATGGCTTTCAACCC-3′ (forward) and 5′-GGGGCTCGAGTTATTTAGCCTTGGCCG-3′ (reverse) for CnMT2. .. The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation).

    DNA Sequencing:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The recombinant plasmids were transformed into the E. coli MachI strain for DNA sequencing, using the Big Dye Terminator 3.1 Cycle Sequencing Kit in an ABIPRISM 310 Automatic Sequencer (Applied Biosystems).

    Expressing:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: Paragraph title: 2.2. Cloning and recombinant expression of the CnMT1 and CnMT2 cDNAs ... The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation).

    Sequencing:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The recombinant plasmids were transformed into the E. coli MachI strain for DNA sequencing, using the Big Dye Terminator 3.1 Cycle Sequencing Kit in an ABIPRISM 310 Automatic Sequencer (Applied Biosystems).

    Gel Extraction:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The final products were analyzed by 0.8% agarose gel electrophoresis and the expected bands were excised (Genelute™ Gel Extraction Kit, Sigma Aldrich). pGEX-4T1 and the amplified inserts were digested with BamH I/ Xho I for CnMT1 and Eco RI/ Xho I for CnMT2, followed, in each case, by a ligation reaction (DNA Ligation Kit 2.1, Takara Bio Inc.).

    Transformation Assay:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation). .. The recombinant plasmids were transformed into the E. coli MachI strain for DNA sequencing, using the Big Dye Terminator 3.1 Cycle Sequencing Kit in an ABIPRISM 310 Automatic Sequencer (Applied Biosystems).

    Recombinant:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: Paragraph title: 2.2. Cloning and recombinant expression of the CnMT1 and CnMT2 cDNAs ... The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation).

    Plasmid Preparation:

    Article Title: Full characterization of the Cu-, Zn-, and Cd-binding properties of CnMT1 and CnMT2, two metallothioneins of the pathogenic fungus Cryptococcus neoformans acting as virulence factors
    Article Snippet: From there, they were subcloned into the BamH I/ Xho I sites for CnMT1, and Eco RI/ Xho I sites for CnMT2 (owing to the presence of an internal BamH I site in CnMT2 ) of the pGEX-4T1 expression vector (GE Healthcare), in order to obtain a GST-MT fusion protein. .. The 30-cycle amplification reactions were performed with the thermo resistant Hotstar Taq DNA polymerase (Qiagen) under the following conditions: 15 min at 95 °C (activation of the DNA polymerase), 30 s at 94 °C (denaturation), 30 s at 55 °C (annealing) and 1 min at 72 °C (elongation).

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  • 90
    Qiagen thermo resistant hotstar taq dna polymerase
    Thermo Resistant Hotstar Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermo resistant hotstar taq dna polymerase/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thermo resistant hotstar taq dna polymerase - by Bioz Stars, 2020-04
    90/100 stars
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