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InGex tgirt iii reverse transcriptase
Tgirt Iii Reverse Transcriptase, supplied by InGex, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tgirt iii reverse transcriptase/product/InGex
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
tgirt iii reverse transcriptase - by Bioz Stars, 2020-04
93/100 stars

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Sequencing:

Article Title: Resetting the yeast epigenome with human nucleosomes
Article Snippet: Sequencing of tRNAs was adapted from ( ) ( ). .. 2 μg total RNA from humanized yeasts was mixed with 100 nM template-primer and 500 nM TGIRT-III reverse-transcriptase (InGex), in reaction medium (450 mM NaCl, 5 mM MgCl2 , 20 mM Tris-HCl pH 7.5, and 5 mM DTT) for 30 min at room temperature.

Purification:

Article Title: Resetting the yeast epigenome with human nucleosomes
Article Snippet: 2 μg total RNA from humanized yeasts was mixed with 100 nM template-primer and 500 nM TGIRT-III reverse-transcriptase (InGex), in reaction medium (450 mM NaCl, 5 mM MgCl2 , 20 mM Tris-HCl pH 7.5, and 5 mM DTT) for 30 min at room temperature. .. Product was purified using a DNA clean and concentrator kit (Zymo).

Concentration Assay:

Article Title: Resetting the yeast epigenome with human nucleosomes
Article Snippet: 2 μg total RNA from humanized yeasts was mixed with 100 nM template-primer and 500 nM TGIRT-III reverse-transcriptase (InGex), in reaction medium (450 mM NaCl, 5 mM MgCl2 , 20 mM Tris-HCl pH 7.5, and 5 mM DTT) for 30 min at room temperature. .. Reactions were terminated by adding 5 M NaOH to a final concentration of 0.25 M, incubating at 95°C for 3 min, and then neutralizing with 5 M HCl.

Incubation:

Article Title: RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications
Article Snippet: RNA was then heat-denatured at 70°C for 5 min and incubated at room temperature for 5 min. .. Reverse transcription was carried out in a final volume of 10 μl, in the presence of 1 mM dNTPs, 10 pmol of SR RT primer (NEBNext® Small RNA Library Prep Set kit), 20 U RNaseOUT™ Recombinant Ribonuclease Inhibitor, and 100 U TGIRT™-III Reverse Transcriptase (InGex), by incubating at 50°C for 5 min, followed by 2 h at 57°C.

Polymerase Chain Reaction:

Article Title: RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications
Article Snippet: Reverse transcription was carried out in a final volume of 10 μl, in the presence of 1 mM dNTPs, 10 pmol of SR RT primer (NEBNext® Small RNA Library Prep Set kit), 20 U RNaseOUT™ Recombinant Ribonuclease Inhibitor, and 100 U TGIRT™-III Reverse Transcriptase (InGex), by incubating at 50°C for 5 min, followed by 2 h at 57°C. .. Template RNA was degraded by adding 1 μl of 5 M NaOH and incubating at 95°C for 3 min. After reaction cleanup, cDNA was eluted in 20 μl nuclease-free water, and barcodes were introduced by 15 cycles of PCR in the presence of 25 pmol of each primer, and 25 μl NEBNext® High-Fidelity 2× PCR Master Mix.

Recombinant:

Article Title: RNA Framework: an all-in-one toolkit for the analysis of RNA structures and post-transcriptional modifications
Article Snippet: .. Reverse transcription was carried out in a final volume of 10 μl, in the presence of 1 mM dNTPs, 10 pmol of SR RT primer (NEBNext® Small RNA Library Prep Set kit), 20 U RNaseOUT™ Recombinant Ribonuclease Inhibitor, and 100 U TGIRT™-III Reverse Transcriptase (InGex), by incubating at 50°C for 5 min, followed by 2 h at 57°C. .. Template RNA was degraded by adding 1 μl of 5 M NaOH and incubating at 95°C for 3 min. After reaction cleanup, cDNA was eluted in 20 μl nuclease-free water, and barcodes were introduced by 15 cycles of PCR in the presence of 25 pmol of each primer, and 25 μl NEBNext® High-Fidelity 2× PCR Master Mix.

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  • 91
    InGex tigrt iii reverse transcriptase
    Tigrt Iii Reverse Transcriptase, supplied by InGex, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tigrt iii reverse transcriptase/product/InGex
    Average 91 stars, based on 1 article reviews
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    InGex tgirt iii reverse transcriptase
    Tgirt Iii Reverse Transcriptase, supplied by InGex, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgirt iii reverse transcriptase/product/InGex
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tgirt iii reverse transcriptase - by Bioz Stars, 2020-04
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    InGex processive thermostable group ii reverse transcriptase enzyme
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