tetramethylrhodamine conjugated transferrin  (Thermo Fisher)


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    Name:
    Tetramethylrhodamine TAMRA Azide Tetramethylrhodamine 5 Carboxamido 6 Azidohexanyl
    Description:
    The red fluorescent tetramethylrhodamine TAMRA azide can be reacted with terminal alkynes via a copper catalyzed click reaction Click chemistry describes a class of chemical reactions that use bio orthogonal or biologically unique moities to label and detect a molecule of interest using a two step procedure The two step reaction procedure involves a copper catalyzed triazole formation of an azide and an alkyne Click reactions have several characteristics the reaction between the detection moieties is efficient no extreme temperatures or solvents are required the reaction product is stable the components of the reaction are bioinert and perhaps most importantly no side reactions occur the label and detection tags react selectively and specifically with one another Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls functional groups that are not unique click chemistry labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background
    Catalog Number:
    t10182
    Price:
    None
    Applications:
    Cell Analysis|Cell Proliferation with Click Chemistry|Click Chemistries|Metabolic Labeling & Nascent Protein Synthesis with Click Chemistry|Labeling Chemistry
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher tetramethylrhodamine conjugated transferrin
    <t>Transferrin</t> internalization in 293/MCAT-1-GFP cells. 293/MCAT-1-GFP cells were bound with <t>tetramethylrhodamine-conjugated</t> transferrin and incubated at 37°C for 30 min. Cells were fixed, and color photomicrographs were produced with a Sony printer connected to the video output of the Zeiss confocal microscope. (Left panel) Transferrin internalization at 37°C for 30 min. (Right panel) SU staining after virus incubation with 293/MCAT-1-GFP cells at 37°C for 30 min.
    The red fluorescent tetramethylrhodamine TAMRA azide can be reacted with terminal alkynes via a copper catalyzed click reaction Click chemistry describes a class of chemical reactions that use bio orthogonal or biologically unique moities to label and detect a molecule of interest using a two step procedure The two step reaction procedure involves a copper catalyzed triazole formation of an azide and an alkyne Click reactions have several characteristics the reaction between the detection moieties is efficient no extreme temperatures or solvents are required the reaction product is stable the components of the reaction are bioinert and perhaps most importantly no side reactions occur the label and detection tags react selectively and specifically with one another Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls functional groups that are not unique click chemistry labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background
    https://www.bioz.com/result/tetramethylrhodamine conjugated transferrin/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tetramethylrhodamine conjugated transferrin - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "Receptor-Mediated Moloney Murine Leukemia Virus Entry Can Occur Independently of the Clathrin-Coated-Pit-Mediated Endocytic Pathway"

    Article Title: Receptor-Mediated Moloney Murine Leukemia Virus Entry Can Occur Independently of the Clathrin-Coated-Pit-Mediated Endocytic Pathway

    Journal: Journal of Virology

    doi:

    Transferrin internalization in 293/MCAT-1-GFP cells. 293/MCAT-1-GFP cells were bound with tetramethylrhodamine-conjugated transferrin and incubated at 37°C for 30 min. Cells were fixed, and color photomicrographs were produced with a Sony printer connected to the video output of the Zeiss confocal microscope. (Left panel) Transferrin internalization at 37°C for 30 min. (Right panel) SU staining after virus incubation with 293/MCAT-1-GFP cells at 37°C for 30 min.
    Figure Legend Snippet: Transferrin internalization in 293/MCAT-1-GFP cells. 293/MCAT-1-GFP cells were bound with tetramethylrhodamine-conjugated transferrin and incubated at 37°C for 30 min. Cells were fixed, and color photomicrographs were produced with a Sony printer connected to the video output of the Zeiss confocal microscope. (Left panel) Transferrin internalization at 37°C for 30 min. (Right panel) SU staining after virus incubation with 293/MCAT-1-GFP cells at 37°C for 30 min.

    Techniques Used: Incubation, Produced, Microscopy, Staining

    Related Articles

    Flow Cytometry:

    Article Title: Mitochondrial pyruvate dehydrogenase phosphatase 1 regulates the early differentiation of cardiomyocytes from mouse embryonic stem cells
    Article Snippet: .. Flow cytometry analyses Cells were stained with 0.1 nM tetramethylrhodamine, methyl ester (TMRM) and 5 μM MitoSox-Red (Invitrogen) for 30 min at 37 °C to detect mitochondrial membrane potential and mitochondrial ROS. .. To measure mitochondrial calcium levels, 5 μM Rhod2-AM (Invitrogen) was applied to dissociated EBs for 2 h on ice, followed by incubation for 30 min at 37 °C.

    Article Title: Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies
    Article Snippet: .. KL25 is an LCMV-WE, and VI10 is a VSV serotype Indiana (IND) neutralizing mAb, described in refs. and , respectively. mAbs were purified from culture supernatant by affinity chromatography (Protein G Sepharose 4 Fast Flow, Amersham Pharmacia Biosciences) and directly labeled with FITC (Fluka), tetramethylrhodamine isothiocyanate (Fluka), or NHS-LC-Biotin (Pierce). .. Generation of Gene-Targeted Mice.

    Affinity Chromatography:

    Article Title: Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies
    Article Snippet: .. KL25 is an LCMV-WE, and VI10 is a VSV serotype Indiana (IND) neutralizing mAb, described in refs. and , respectively. mAbs were purified from culture supernatant by affinity chromatography (Protein G Sepharose 4 Fast Flow, Amersham Pharmacia Biosciences) and directly labeled with FITC (Fluka), tetramethylrhodamine isothiocyanate (Fluka), or NHS-LC-Biotin (Pierce). .. Generation of Gene-Targeted Mice.

    Cytometry:

    Article Title: Mitochondrial pyruvate dehydrogenase phosphatase 1 regulates the early differentiation of cardiomyocytes from mouse embryonic stem cells
    Article Snippet: .. Flow cytometry analyses Cells were stained with 0.1 nM tetramethylrhodamine, methyl ester (TMRM) and 5 μM MitoSox-Red (Invitrogen) for 30 min at 37 °C to detect mitochondrial membrane potential and mitochondrial ROS. .. To measure mitochondrial calcium levels, 5 μM Rhod2-AM (Invitrogen) was applied to dissociated EBs for 2 h on ice, followed by incubation for 30 min at 37 °C.

    Labeling:

    Article Title: Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies
    Article Snippet: .. KL25 is an LCMV-WE, and VI10 is a VSV serotype Indiana (IND) neutralizing mAb, described in refs. and , respectively. mAbs were purified from culture supernatant by affinity chromatography (Protein G Sepharose 4 Fast Flow, Amersham Pharmacia Biosciences) and directly labeled with FITC (Fluka), tetramethylrhodamine isothiocyanate (Fluka), or NHS-LC-Biotin (Pierce). .. Generation of Gene-Targeted Mice.

    Article Title: The S1P-analog FTY720 differentially modulates T-cell homing via HEV: T-cell-expressed S1P1 amplifies integrin activation in peripheral lymph nodes but not in Peyer patches
    Article Snippet: .. For competitive homing experiments, S1P1 –/– and S1P1 +/ thymocytes or splenic B cells from FL chimeras were labeled with TRITC or carboxy-fluorescein diacetate, succinimidyl ester (CFSE; Molecular Probes) as described and coinjected intravenously into recipient mice. .. Single-cell suspensions were stained with anti–CD4–peridinin chlorophyll protein (PerCP) and anti–CD8α-allophycocyanin (APC) or anti-B220-PerCP and analyzed by fluorescence activated cell sorting (FACS).

    Purification:

    Article Title: Antiviral immune responses in gene-targeted mice expressing the immunoglobulin heavy chain of virus-neutralizing antibodies
    Article Snippet: .. KL25 is an LCMV-WE, and VI10 is a VSV serotype Indiana (IND) neutralizing mAb, described in refs. and , respectively. mAbs were purified from culture supernatant by affinity chromatography (Protein G Sepharose 4 Fast Flow, Amersham Pharmacia Biosciences) and directly labeled with FITC (Fluka), tetramethylrhodamine isothiocyanate (Fluka), or NHS-LC-Biotin (Pierce). .. Generation of Gene-Targeted Mice.

    Incubation:

    Article Title: Glial Cell Line-Derived Neurotrophic Factor Administration in Postnatal Life Results in Motor Unit Enlargement and Continuous Synaptic Remodeling at the Neuromuscular Junction
    Article Snippet: .. Sternomastoid muscles from CF1 and spinotrapezius muscles from thy1-YFP mice were incubated for 30 min at room temperature in 5 μg/ml tetramethylrhodamine-conjugated α-bungarotoxin (Molecular Probes, Eugene, OR) that was diluted in 1% bovine serum albumin (BSA) in sterile lactated Ringer's solution. ..

    Staining:

    Article Title: Mitochondrial pyruvate dehydrogenase phosphatase 1 regulates the early differentiation of cardiomyocytes from mouse embryonic stem cells
    Article Snippet: .. Flow cytometry analyses Cells were stained with 0.1 nM tetramethylrhodamine, methyl ester (TMRM) and 5 μM MitoSox-Red (Invitrogen) for 30 min at 37 °C to detect mitochondrial membrane potential and mitochondrial ROS. .. To measure mitochondrial calcium levels, 5 μM Rhod2-AM (Invitrogen) was applied to dissociated EBs for 2 h on ice, followed by incubation for 30 min at 37 °C.

    Article Title: Symbiotic Relationship between Streptococcus mutans and Candida albicans Synergizes Virulence of Plaque Biofilms In Vivo
    Article Snippet: .. Based on work from other groups ( , ), we elected to stain the yeast using concanavalin A (ConA) lectin conjugated with tetramethylrhodamine (absorbance/fluorescence emission maxima, 555/580 nm; Molecular Probes). .. We used a concentration of 40 μg/ml with an incubation time of 30 min to reduce the possibility that ConA might bind S. mutans , since ConA is capable of agglutinating several serotypes of S. mutans , but not serotype c strains ( ).

    Mouse Assay:

    Article Title: Glial Cell Line-Derived Neurotrophic Factor Administration in Postnatal Life Results in Motor Unit Enlargement and Continuous Synaptic Remodeling at the Neuromuscular Junction
    Article Snippet: .. Sternomastoid muscles from CF1 and spinotrapezius muscles from thy1-YFP mice were incubated for 30 min at room temperature in 5 μg/ml tetramethylrhodamine-conjugated α-bungarotoxin (Molecular Probes, Eugene, OR) that was diluted in 1% bovine serum albumin (BSA) in sterile lactated Ringer's solution. ..

    Article Title: The S1P-analog FTY720 differentially modulates T-cell homing via HEV: T-cell-expressed S1P1 amplifies integrin activation in peripheral lymph nodes but not in Peyer patches
    Article Snippet: .. For competitive homing experiments, S1P1 –/– and S1P1 +/ thymocytes or splenic B cells from FL chimeras were labeled with TRITC or carboxy-fluorescein diacetate, succinimidyl ester (CFSE; Molecular Probes) as described and coinjected intravenously into recipient mice. .. Single-cell suspensions were stained with anti–CD4–peridinin chlorophyll protein (PerCP) and anti–CD8α-allophycocyanin (APC) or anti-B220-PerCP and analyzed by fluorescence activated cell sorting (FACS).