terrific broth medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher terrific broth medium
    Terrific Broth Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terrific broth medium/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    terrific broth medium - by Bioz Stars, 2020-04
    95/100 stars

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    Centrifugation:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C. .. After growing at 16°C overnight, the cells were pelleted down by centrifugation at 8,000 × g for 10 min at 4°C and resuspended in a lysis buffer (pH 7.5, 10 mM Na2 HPO4 , 1.8 mM KH2 PO4 , 2.7 mM KCl, 400 mM NaCl, 1 mM EDTA, 10 U/ml Benzonase nuclease [Millipore], and EDTA-free protease inhibitor cocktail [Roche]), followed by ultrasonication at 4°C.

    Protease Inhibitor:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C. .. After growing at 16°C overnight, the cells were pelleted down by centrifugation at 8,000 × g for 10 min at 4°C and resuspended in a lysis buffer (pH 7.5, 10 mM Na2 HPO4 , 1.8 mM KH2 PO4 , 2.7 mM KCl, 400 mM NaCl, 1 mM EDTA, 10 U/ml Benzonase nuclease [Millipore], and EDTA-free protease inhibitor cocktail [Roche]), followed by ultrasonication at 4°C.

    Cell Culture:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: .. The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C. .. Once the cell culture reached an optical density at 600 nm (OD600 ) of 1.0, isopropyl-β-d -thiogalactopyranoside (IPTG) was added to a final concentration of 0.5 mM to induce protein expression.

    Purification:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: Paragraph title: Expression and purification of recombinant ZIKV NS2A proteins in E. coli . ... The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C.

    Concentration Assay:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C. .. Once the cell culture reached an optical density at 600 nm (OD600 ) of 1.0, isopropyl-β-d -thiogalactopyranoside (IPTG) was added to a final concentration of 0.5 mM to induce protein expression.

    Expressing:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: Paragraph title: Expression and purification of recombinant ZIKV NS2A proteins in E. coli . ... The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C.

    Lysis:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C. .. After growing at 16°C overnight, the cells were pelleted down by centrifugation at 8,000 × g for 10 min at 4°C and resuspended in a lysis buffer (pH 7.5, 10 mM Na2 HPO4 , 1.8 mM KH2 PO4 , 2.7 mM KCl, 400 mM NaCl, 1 mM EDTA, 10 U/ml Benzonase nuclease [Millipore], and EDTA-free protease inhibitor cocktail [Roche]), followed by ultrasonication at 4°C.

    Transformation Assay:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: In brief, pGEX-4T-1-derived plasmids encoding the corresponding GST-tagged NS2A or mutants were transformed into E. coli strain Rosetta 2(DE3)pLysS cells (Novagen). .. The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C.

    Recombinant:

    Article Title: Zika Virus NS2A-Mediated Virion Assembly
    Article Snippet: Paragraph title: Expression and purification of recombinant ZIKV NS2A proteins in E. coli . ... The cells were cultured in Terrific Broth medium (Invitrogen) with 100 μg/ml ampicillin and 34 μg/ml chloramphenicol at 37°C.

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  • 99
    Thermo Fisher terrific broth medium
    Terrific Broth Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terrific broth medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    terrific broth medium - by Bioz Stars, 2020-04
    99/100 stars
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