Termipol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae"
Article Title: Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae
Journal: Frontiers in Microbiology
Figure Legend Snippet: The principal scheme of sport transformation . Amplified DNA fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.
Techniques Used: Transformation Assay, Amplification, Mutagenesis, Incubation, Single-particle Tracking
2) Product Images from "A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation"
Article Title: A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation
Journal: Epigenetics & Chromatin
Figure Legend Snippet: MR-SNuPE assay design for SEPT9 and general performance . (A) SEPT9 amplicon sequence with indicated primer (boxes) and blocker (line above) positions. (B) Electropherograms of separated single nucleotide primer extension (SNuPE) products from PCR products obtained without blocker on (a) completely methylated and (b) unmethylated DNA templates or (c-f) mixed DNA templates (methylated:unmethylated DNA ratios/dilution series). (g) Effect when performing a heavy methyl (HM)-PCR; that is, when the blocker is included, on a dilution shown in (f) . Vertical dashed lines indicate the positions of unextended primer, methylated and unmethylated signals, respectively, NTC = no template control, that is, SNuPE reaction without PCR template.
Techniques Used: Snupe Assay, Amplification, Sequencing, Polymerase Chain Reaction, Methylation
Figure Legend Snippet: Assay performance tested on samples from patients with colorectal cancer . Electropherograms after separation of methylation-restricted single nucleotide primer extension (MR-SNuPE) products obtained from plasma taken from patients with colonoscopy-verified colorectal cancer. Plasma from (a-c) patient with cancer, obtained from Proteogenex (PRO6, PRO20) or Oncomatrix (OMA19); (d) healthy individual, obtained from Oncomatrix (OMA8). (e) Normal blood plasma (5 ml) spiked with 12.5 ng methylated DNA (Chem). Peaks were assessed by the relative signal retention times as described in the legend to Figure 2. UP = unextended primer, M = methylated signal; NTC = SNuPE reaction with water.
Techniques Used: Methylation