termipol dna polymerase  (Solis BioDyne)


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    Solis BioDyne termipol dna polymerase
    The principal scheme of sport transformation . Amplified <t>DNA</t> fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.
    Termipol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/termipol dna polymerase/product/Solis BioDyne
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    termipol dna polymerase - by Bioz Stars, 2022-07
    86/100 stars

    Images

    1) Product Images from "Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae"

    Article Title: Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2013.00186

    The principal scheme of sport transformation . Amplified DNA fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.
    Figure Legend Snippet: The principal scheme of sport transformation . Amplified DNA fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.

    Techniques Used: Transformation Assay, Amplification, Mutagenesis, Incubation, Single-particle Tracking

    2) Product Images from "A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation"

    Article Title: A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation

    Journal: Epigenetics & Chromatin

    doi: 10.1186/1756-8935-3-12

    MR-SNuPE assay design for SEPT9 and general performance . (A) SEPT9 amplicon sequence with indicated primer (boxes) and blocker (line above) positions. (B) Electropherograms of separated single nucleotide primer extension (SNuPE) products from PCR products obtained without blocker on (a) completely methylated and (b) unmethylated DNA templates or (c-f) mixed DNA templates (methylated:unmethylated DNA ratios/dilution series). (g) Effect when performing a heavy methyl (HM)-PCR; that is, when the blocker is included, on a dilution shown in (f) . Vertical dashed lines indicate the positions of unextended primer, methylated and unmethylated signals, respectively, NTC = no template control, that is, SNuPE reaction without PCR template.
    Figure Legend Snippet: MR-SNuPE assay design for SEPT9 and general performance . (A) SEPT9 amplicon sequence with indicated primer (boxes) and blocker (line above) positions. (B) Electropherograms of separated single nucleotide primer extension (SNuPE) products from PCR products obtained without blocker on (a) completely methylated and (b) unmethylated DNA templates or (c-f) mixed DNA templates (methylated:unmethylated DNA ratios/dilution series). (g) Effect when performing a heavy methyl (HM)-PCR; that is, when the blocker is included, on a dilution shown in (f) . Vertical dashed lines indicate the positions of unextended primer, methylated and unmethylated signals, respectively, NTC = no template control, that is, SNuPE reaction without PCR template.

    Techniques Used: Snupe Assay, Amplification, Sequencing, Polymerase Chain Reaction, Methylation

    Assay performance tested on samples from patients with colorectal cancer . Electropherograms after separation of methylation-restricted single nucleotide primer extension (MR-SNuPE) products obtained from plasma taken from patients with colonoscopy-verified colorectal cancer. Plasma from (a-c) patient with cancer, obtained from Proteogenex (PRO6, PRO20) or Oncomatrix (OMA19); (d) healthy individual, obtained from Oncomatrix (OMA8). (e) Normal blood plasma (5 ml) spiked with 12.5 ng methylated DNA (Chem). Peaks were assessed by the relative signal retention times as described in the legend to Figure 2. UP = unextended primer, M = methylated signal; NTC = SNuPE reaction with water.
    Figure Legend Snippet: Assay performance tested on samples from patients with colorectal cancer . Electropherograms after separation of methylation-restricted single nucleotide primer extension (MR-SNuPE) products obtained from plasma taken from patients with colonoscopy-verified colorectal cancer. Plasma from (a-c) patient with cancer, obtained from Proteogenex (PRO6, PRO20) or Oncomatrix (OMA19); (d) healthy individual, obtained from Oncomatrix (OMA8). (e) Normal blood plasma (5 ml) spiked with 12.5 ng methylated DNA (Chem). Peaks were assessed by the relative signal retention times as described in the legend to Figure 2. UP = unextended primer, M = methylated signal; NTC = SNuPE reaction with water.

    Techniques Used: Methylation

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    Solis BioDyne dna polymerases
    Examples of Silhouette scores. Examples of genotype clusters from nine SNP assays, each with the results from 16 samples genotyped in duplicate using Tag-array minisequencing with the calculated Silhouette scores shown in the right hand upper corner of each panel. The blue circles represent homozygotes for allele 2, the red triangles are heterozygotes and the green squares are homozygotes for allele 1. The SNPs are denoted by their dbSNP identification number, and the <t>DNA</t> polarities analyzed are indicated by
    Dna Polymerases, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerases/product/Solis BioDyne
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Solis BioDyne termipol dna polymerase
    The principal scheme of sport transformation . Amplified <t>DNA</t> fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.
    Termipol Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/termipol dna polymerase/product/Solis BioDyne
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    termipol dna polymerase - by Bioz Stars, 2022-07
    86/100 stars
      Buy from Supplier

    86
    Solis BioDyne sequenase
    Schematic representation of the FlexiChip analysis method . A) SNPs are detected by Single Base Extension (SBE) using <t>Sequenase</t> and ddNTP labelled with Cyanine 3 or 5 using a specific probe that hybridizes one nucleotide upstream of the SNP site. B) Products of the SBE reaction are hybridized on FlexiChip by their Zip-code oligonucleotides. After washing and drying, the slides are scanned at two wave lengths. C) Analysis algorithm is based on a mixture model and allows accurate SNP identification. The results are stored in Excel file.
    Sequenase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequenase/product/Solis BioDyne
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequenase - by Bioz Stars, 2022-07
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    Examples of Silhouette scores. Examples of genotype clusters from nine SNP assays, each with the results from 16 samples genotyped in duplicate using Tag-array minisequencing with the calculated Silhouette scores shown in the right hand upper corner of each panel. The blue circles represent homozygotes for allele 2, the red triangles are heterozygotes and the green squares are homozygotes for allele 1. The SNPs are denoted by their dbSNP identification number, and the DNA polarities analyzed are indicated by

    Journal: BMC Genomics

    Article Title: Silhouette scores for assessment of SNP genotype clusters

    doi: 10.1186/1471-2164-6-35

    Figure Lengend Snippet: Examples of Silhouette scores. Examples of genotype clusters from nine SNP assays, each with the results from 16 samples genotyped in duplicate using Tag-array minisequencing with the calculated Silhouette scores shown in the right hand upper corner of each panel. The blue circles represent homozygotes for allele 2, the red triangles are heterozygotes and the green squares are homozygotes for allele 1. The SNPs are denoted by their dbSNP identification number, and the DNA polarities analyzed are indicated by "cod" or "nc".

    Article Snippet: The DNA polymerases were; TERMIPol (Solis BioDyne, Tartu, Estonia), Therminator (New England BioLabs Inc., Beverly, MA, USA), KlenThermase (Gene Craft, Lüdinghausen, Germany), or ThermoSequenase (Amersham Biosciences, Uppsala, Sweden).

    Techniques:

    Distribution of Silhouette scores from minisequencing assays using four DNA polymerases. The Silhouette score is given on the y-axis. Each black diamond represents the Silhouette score for one SNP assay. The light blue rectangular boxes indicate those 75% of the scatter plots that yielded the highest silhouette scores for each enzyme. Quartiles are indicated by the black horizontal lines.

    Journal: BMC Genomics

    Article Title: Silhouette scores for assessment of SNP genotype clusters

    doi: 10.1186/1471-2164-6-35

    Figure Lengend Snippet: Distribution of Silhouette scores from minisequencing assays using four DNA polymerases. The Silhouette score is given on the y-axis. Each black diamond represents the Silhouette score for one SNP assay. The light blue rectangular boxes indicate those 75% of the scatter plots that yielded the highest silhouette scores for each enzyme. Quartiles are indicated by the black horizontal lines.

    Article Snippet: The DNA polymerases were; TERMIPol (Solis BioDyne, Tartu, Estonia), Therminator (New England BioLabs Inc., Beverly, MA, USA), KlenThermase (Gene Craft, Lüdinghausen, Germany), or ThermoSequenase (Amersham Biosciences, Uppsala, Sweden).

    Techniques:

    The principal scheme of sport transformation . Amplified DNA fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.

    Journal: Frontiers in Microbiology

    Article Title: Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae

    doi: 10.3389/fmicb.2013.00186

    Figure Lengend Snippet: The principal scheme of sport transformation . Amplified DNA fragments carried mutant (mut) and wild type (wt) of rpsE gene cording the ribosomal protein S5 (RPS5) were placed in spots on a GC base agar plate. Then piliated colonies of recipient strains (NG7 and NG94) were streaked across the plate through the DNA spots. After overnight incubation, bacterial cells were swabbed from the spots into a liquid medium, diluted and plated on both GC base agar alone, and GC base agar supplemented with 64 mg/L of spectinomycin (SPT). The individual transformants emerged on SPT-supplemented plates were picked up for further examination. Colony-forming units (CFUs) were counted on each plate.

    Article Snippet: After that amplicon was used as template in thermocyclic primer extension reaction, carried out in 20 μ L of the mixture of 66 mM Tris-HCl pH 9.0; 16.6 mM (NH4 )2 SO4 ; 2.5 mM MgCl2; 0.2 mM of each dGTP and ddTTP, 10 pmol of internal primer (see Table ) and 2 units of TermiPol DNA Polymerase (Solis Biodyne, Estonia) according to the followed profiling: 94°C for 20 s, 58°C for 20 s, and 72°C for 15 s, in 70 cycles.

    Techniques: Transformation Assay, Amplification, Mutagenesis, Incubation, Single-particle Tracking

    MR-SNuPE assay design for SEPT9 and general performance . (A) SEPT9 amplicon sequence with indicated primer (boxes) and blocker (line above) positions. (B) Electropherograms of separated single nucleotide primer extension (SNuPE) products from PCR products obtained without blocker on (a) completely methylated and (b) unmethylated DNA templates or (c-f) mixed DNA templates (methylated:unmethylated DNA ratios/dilution series). (g) Effect when performing a heavy methyl (HM)-PCR; that is, when the blocker is included, on a dilution shown in (f) . Vertical dashed lines indicate the positions of unextended primer, methylated and unmethylated signals, respectively, NTC = no template control, that is, SNuPE reaction without PCR template.

    Journal: Epigenetics & Chromatin

    Article Title: A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation

    doi: 10.1186/1756-8935-3-12

    Figure Lengend Snippet: MR-SNuPE assay design for SEPT9 and general performance . (A) SEPT9 amplicon sequence with indicated primer (boxes) and blocker (line above) positions. (B) Electropherograms of separated single nucleotide primer extension (SNuPE) products from PCR products obtained without blocker on (a) completely methylated and (b) unmethylated DNA templates or (c-f) mixed DNA templates (methylated:unmethylated DNA ratios/dilution series). (g) Effect when performing a heavy methyl (HM)-PCR; that is, when the blocker is included, on a dilution shown in (f) . Vertical dashed lines indicate the positions of unextended primer, methylated and unmethylated signals, respectively, NTC = no template control, that is, SNuPE reaction without PCR template.

    Article Snippet: For SEPT9, to the PCR product/Exo-SAP mix, 2 μl of 10× buffer C (Solis BioDyne), 2.4 μl of 30 μM SNuPE primer (oligos 27, 28 and 45), 1 μl of 1 mM ddCTP and ddTTP or ddGTP and ddATP, respectively, and 0.5 μl of Termipol DNA polymerase (5 U/μl, Solis BioDyne) were added to reach a final volume of 20 μl.

    Techniques: Snupe Assay, Amplification, Sequencing, Polymerase Chain Reaction, Methylation

    Assay performance tested on samples from patients with colorectal cancer . Electropherograms after separation of methylation-restricted single nucleotide primer extension (MR-SNuPE) products obtained from plasma taken from patients with colonoscopy-verified colorectal cancer. Plasma from (a-c) patient with cancer, obtained from Proteogenex (PRO6, PRO20) or Oncomatrix (OMA19); (d) healthy individual, obtained from Oncomatrix (OMA8). (e) Normal blood plasma (5 ml) spiked with 12.5 ng methylated DNA (Chem). Peaks were assessed by the relative signal retention times as described in the legend to Figure 2. UP = unextended primer, M = methylated signal; NTC = SNuPE reaction with water.

    Journal: Epigenetics & Chromatin

    Article Title: A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation

    doi: 10.1186/1756-8935-3-12

    Figure Lengend Snippet: Assay performance tested on samples from patients with colorectal cancer . Electropherograms after separation of methylation-restricted single nucleotide primer extension (MR-SNuPE) products obtained from plasma taken from patients with colonoscopy-verified colorectal cancer. Plasma from (a-c) patient with cancer, obtained from Proteogenex (PRO6, PRO20) or Oncomatrix (OMA19); (d) healthy individual, obtained from Oncomatrix (OMA8). (e) Normal blood plasma (5 ml) spiked with 12.5 ng methylated DNA (Chem). Peaks were assessed by the relative signal retention times as described in the legend to Figure 2. UP = unextended primer, M = methylated signal; NTC = SNuPE reaction with water.

    Article Snippet: For SEPT9, to the PCR product/Exo-SAP mix, 2 μl of 10× buffer C (Solis BioDyne), 2.4 μl of 30 μM SNuPE primer (oligos 27, 28 and 45), 1 μl of 1 mM ddCTP and ddTTP or ddGTP and ddATP, respectively, and 0.5 μl of Termipol DNA polymerase (5 U/μl, Solis BioDyne) were added to reach a final volume of 20 μl.

    Techniques: Methylation

    Schematic representation of the FlexiChip analysis method . A) SNPs are detected by Single Base Extension (SBE) using Sequenase and ddNTP labelled with Cyanine 3 or 5 using a specific probe that hybridizes one nucleotide upstream of the SNP site. B) Products of the SBE reaction are hybridized on FlexiChip by their Zip-code oligonucleotides. After washing and drying, the slides are scanned at two wave lengths. C) Analysis algorithm is based on a mixture model and allows accurate SNP identification. The results are stored in Excel file.

    Journal: Malaria Journal

    Article Title: FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance

    doi: 10.1186/1475-2875-8-229

    Figure Lengend Snippet: Schematic representation of the FlexiChip analysis method . A) SNPs are detected by Single Base Extension (SBE) using Sequenase and ddNTP labelled with Cyanine 3 or 5 using a specific probe that hybridizes one nucleotide upstream of the SNP site. B) Products of the SBE reaction are hybridized on FlexiChip by their Zip-code oligonucleotides. After washing and drying, the slides are scanned at two wave lengths. C) Analysis algorithm is based on a mixture model and allows accurate SNP identification. The results are stored in Excel file.

    Article Snippet: Sequenase (Termipol® , Solis, Tartu, Estonia) extension reaction, reaction mixture and final denaturation were done for ResMalChip and FlexiChip as described by Crameri et al [ ].

    Techniques: