Structured Review

TaKaRa technology kit
Technology Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/technology kit/product/TaKaRa
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
technology kit - by Bioz Stars, 2020-04
93/100 stars

Images

Related Articles

Clone Assay:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. Cells expressing the MBP-fused zCcd1 DIX domain were suspended in 50 mM Na HEPES buffer (pH 7.5) containing 150 mM NaCl, 1 mM dithiothreitol (DTT), and 1% TritonX-100 and disrupted by ultrasonication at 277 K. The supernatant was loaded onto an amylose resin column (New England Biolabs) and the MBP-fused zCcd1 DIX was eluted with 50 mM HEPES-Na (pH 7.5) containing 150 mM NaCl, 1 mM DTT, and 20 mM maltose.

Amplification:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. Cells expressing the MBP-fused zCcd1 DIX domain were suspended in 50 mM Na HEPES buffer (pH 7.5) containing 150 mM NaCl, 1 mM dithiothreitol (DTT), and 1% TritonX-100 and disrupted by ultrasonication at 277 K. The supernatant was loaded onto an amylose resin column (New England Biolabs) and the MBP-fused zCcd1 DIX was eluted with 50 mM HEPES-Na (pH 7.5) containing 150 mM NaCl, 1 mM DTT, and 20 mM maltose.

Sample Prep:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Paragraph title: Sample preparation ... Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) .

Mutagenesis:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Mutants were constructed using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) and confirmed by DNA sequencing. .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) .

Construct:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Mutants were constructed using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) and confirmed by DNA sequencing. .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) .

Purification:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: DIX domains of mCcd1 was purified and crystallized as described earlier , . .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) .

Incubation:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. To cleave the MBP tag, the purified MBP-fused zCcd1 DIX domain was incubated with an excess molar ratio of the GST-fused HRV3C protease at 284 K overnight and applied to a HiTrap Q high performance column (GE Healthcare) equilibrated with a loading buffer consisting of 20 mM HEPES-Na (pH 7.5), 150 mM NaCl, and 1 mM DTT.

DNA Sequencing:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Mutants were constructed using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) and confirmed by DNA sequencing. .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) .

Expressing:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. Cells expressing the MBP-fused zCcd1 DIX domain were suspended in 50 mM Na HEPES buffer (pH 7.5) containing 150 mM NaCl, 1 mM dithiothreitol (DTT), and 1% TritonX-100 and disrupted by ultrasonication at 277 K. The supernatant was loaded onto an amylose resin column (New England Biolabs) and the MBP-fused zCcd1 DIX was eluted with 50 mM HEPES-Na (pH 7.5) containing 150 mM NaCl, 1 mM DTT, and 20 mM maltose.

Polymerase Chain Reaction:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. Cells expressing the MBP-fused zCcd1 DIX domain were suspended in 50 mM Na HEPES buffer (pH 7.5) containing 150 mM NaCl, 1 mM dithiothreitol (DTT), and 1% TritonX-100 and disrupted by ultrasonication at 277 K. The supernatant was loaded onto an amylose resin column (New England Biolabs) and the MBP-fused zCcd1 DIX was eluted with 50 mM HEPES-Na (pH 7.5) containing 150 mM NaCl, 1 mM DTT, and 20 mM maltose.

Plasmid Preparation:

Article Title: Structural basis for Ccd1 auto-inhibition in the Wnt pathway through homomerization of the DIX domain
Article Snippet: .. Nucleotide fragments encoding the DIX domain of zCcd1 were amplified by PCR using primers designed based on cDNA (Gene ID 360137) and the amplified fragment was subcloned into the pOPINM plasmid (Addgene) using the in-Fusion HD cloning technology kit (Clontech Laboratories, Inc.) . .. Cells expressing the MBP-fused zCcd1 DIX domain were suspended in 50 mM Na HEPES buffer (pH 7.5) containing 150 mM NaCl, 1 mM dithiothreitol (DTT), and 1% TritonX-100 and disrupted by ultrasonication at 277 K. The supernatant was loaded onto an amylose resin column (New England Biolabs) and the MBP-fused zCcd1 DIX was eluted with 50 mM HEPES-Na (pH 7.5) containing 150 mM NaCl, 1 mM DTT, and 20 mM maltose.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa template switching technology smart seq v4 ultra low input rna kit
    Template Switching Technology Smart Seq V4 Ultra Low Input Rna Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template switching technology smart seq v4 ultra low input rna kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    template switching technology smart seq v4 ultra low input rna kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    TaKaRa technology kit
    Technology Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/technology kit/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    technology kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    85
    TaKaRa smart technology kit
    Smart Technology Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smart technology kit/product/TaKaRa
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    smart technology kit - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results