Journal: BMC Cancer

Article Title: Role of Cancer Associated Fibroblast (CAF) derived miRNAs on head and neck malignancies microenvironment: a systematic review

doi: 10.1186/s12885-025-13965-9

Figure Lengend Snippet: Summary characteristics of included studies

Article Snippet: Koji Tanaka – et al. [ ] , 2015 , Japan , in vivo (human) and In vitro (human) , esophageal squamous cell carcinoma (escc) , An in vivo case The study involved 64 patients who received neoadjuvant chemotherapy and underwent surgery for primary esophageal squamous cell carcinoma at the Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University In vivo control sample: 27 healthy participants Sample case in vitro: The verified human esophageal squamous cell line TE10 was acquired from the Riken Bioresource Center Cell Bank in Tsukuba, Japan , 1. Assessment of the reaction to chemotherapy 2. Isolation and characterization of fibroblasts 3. Cell lines and culture conditions 4. RNA isolation 5. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for measuring miRNA levels 6. Quantitative real-time polymerase chain reaction 7. miRNA transfection 8. microRNA array 9. Immunofluorescence staining 10. Conducting growth inhibition experiments using cisplatin therapy and apoptosis assay 11. ELISA 12. Western blotting 13. Statistical analysis , • Increased levels of miR-27a/b expression correlated with a poor response to chemotherapy in patients with esophageal cancer • Introducing miR-27a/b into cancer cells did not substantially impact their reaction to chemotherapy • Esophageal cancer cells cultured in the medium surrounding normal fibroblasts transfected with miR-27a/b exhibited reduced responsiveness to cisplatin compared to cells cultured in the medium surrounding normal fibroblasts • Transfected normal fibroblasts with MiR-27a/b showed elevated α-smooth muscle actin (α-SMA) expression, a characteristic of cancer-associated fibroblasts (CAF), and increased levels of transforming growth factor-β (TGF-β) , miR-27a/b contributes to chemotherapy resistance in esophageal cancer by inducing the conversion of normal fibroblasts into cancer-associated fibroblasts (CAFs).

Techniques: In Vitro, Control, Formalin-fixed Paraffin-Embedded, Expressing, Quantitative RT-PCR, Cell Culture, Transfection, Western Blot, Migration, Biomarker Discovery, Isolation, Purification, Reverse Transcription, Polymerase Chain Reaction, Over Expression, Labeling, Transmission Assay, Electron Microscopy, RNA Extraction, Sequencing, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Immunofluorescence, Immunohistochemistry, Luciferase, Gene Expression, Disruption, Reporter Assay, Immunodepletion, Amplification, Immunocytochemistry, Flow Cytometry, Derivative Assay, Immunohistochemical staining, Extraction, Microarray, Contraction Assay, Comparison, In Vivo, Ex Vivo, Staining, Invasion Assay, Tube Formation Assay, Fluorescence, In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Inhibition, Apoptosis Assay, Enzyme-linked Immunosorbent Assay, Produced, Phospho-proteomics, Transformation Assay, Activation Assay, Cell Isolation, Transduction, Stable Transfection, DNA Methylation Assay, Cell Migration Assay, Plasmid Preparation, Zymography, Functional Assay, Translocation Assay, Clinical Proteomics, Construct, MTT Assay, Co-culture Assay, Pull Down Assay, Immunoprecipitation, Tumorigenicity Assay, Transferring, RNA Sequencing, Knockdown, Small Interfering RNA, Immunostaining, Protein-Protein interactions, Gradient Centrifugation, CCK-8 Assay, Injection, Protein Extraction