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Fig. 4. Inhibition of galectin-3 represses microglial activation in KA-treated microglia. a–f BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM <t>TD139</t> for 24 h. a–c The Iba1 expression was analyzed by immunofluorescence. d–f The protein expression of galectin-3 and Iba1 was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.
Ep Td139 Group, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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td-2131 (phenol novolac resin, hydroxyl group equivalent: 104 g/eq)  (Dainippon Sumitomo)
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Fig. 4. Inhibition of galectin-3 represses microglial activation in KA-treated microglia. a–f BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM <t>TD139</t> for 24 h. a–c The Iba1 expression was analyzed by immunofluorescence. d–f The protein expression of galectin-3 and Iba1 was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.
Td 2131 (Phenol Novolac Resin, Hydroxyl Group Equivalent: 104 G/Eq), supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. Inhibition of galectin-3 represses microglial activation in KA-treated microglia. a–f BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM TD139 for 24 h. a–c The Iba1 expression was analyzed by immunofluorescence. d–f The protein expression of galectin-3 and Iba1 was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Neuroimmunomodulation

Article Title: Inhibition of Galectin-3 in a Rat Model of Epilepsy and Kainate-Activated BV2 Cells Limits Microglial Activation Through the NLRP3/Pyroptosis Pathway.

doi: 10.1159/000534833

Figure Lengend Snippet: Fig. 4. Inhibition of galectin-3 represses microglial activation in KA-treated microglia. a–f BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM TD139 for 24 h. a–c The Iba1 expression was analyzed by immunofluorescence. d–f The protein expression of galectin-3 and Iba1 was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The EP model was successfully established when the seizure activity reached ≥ grade IV and continued for >30 min. For the EP + TD139 group, EP rats were injected intraperitoneally with TD139 (purity >98%; dissolve in dimethylsulfoxide; S0471, Selleck) at 8 mg/kg/d for 10 days starting 4 h after a seizure.

Techniques: Inhibition, Activation Assay, Transfection, shRNA, Expressing, Western Blot

Fig. 5. Inhibition of galectin-3 represses the activation of NLRP3 inflammasome in KA-treated microglia. a–c BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM TD139 for 24 h. The expression of NLRP3, ASC, C-caspase-1, and GSDMD-N was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Neuroimmunomodulation

Article Title: Inhibition of Galectin-3 in a Rat Model of Epilepsy and Kainate-Activated BV2 Cells Limits Microglial Activation Through the NLRP3/Pyroptosis Pathway.

doi: 10.1159/000534833

Figure Lengend Snippet: Fig. 5. Inhibition of galectin-3 represses the activation of NLRP3 inflammasome in KA-treated microglia. a–c BV2 cells or primary microglia were treated with 100 μM KA for 24 h. For inhibition of galectin-3, BV2 cells or primary microglia were transfected with lentivirus vectors carrying Lgals3 shRNA or treated with 20 μM TD139 for 24 h. The expression of NLRP3, ASC, C-caspase-1, and GSDMD-N was analyzed by Western blot (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The EP model was successfully established when the seizure activity reached ≥ grade IV and continued for >30 min. For the EP + TD139 group, EP rats were injected intraperitoneally with TD139 (purity >98%; dissolve in dimethylsulfoxide; S0471, Selleck) at 8 mg/kg/d for 10 days starting 4 h after a seizure.

Techniques: Inhibition, Activation Assay, Transfection, shRNA, Expressing, Western Blot