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Sangon Biotech tbst
Tbst, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Sangon Biotech
Average 92 stars, based on 27 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Incubation:

Article Title: High blood sugar levels but not diabetes mellitus significantly enhance oxaliplatin chemoresistance in patients with stage III colorectal cancer receiving adjuvant FOLFOX6 chemotherapy
Article Snippet: .. Membranes were washed three times with TBST and incubated with a goat anti-rabbit secondary antibody (Sangon Biotech, Shanghai, China) for 2 h and washed three times with TBST. .. Immunoblot results were quantified using AlphaImager software (Alpha Innotech, San Leandro, CA, USA).

Article Title: Phosphorus Deficiency Inhibits Cell Division But Not Growth in the Dinoflagellate Amphidinium carterae
Article Snippet: .. The resolved proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immun-Blot® PVDF Membrane for Protein Blotting, Cat. #162-0177, Bio-Rad, USA) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA) at 25 V for 30 min. After the transfer, the membranes were blocked in 5% defatted milk–TBST solution [Tris buffered saline (TBS) containing 0.1% Tween-20] at 4°C overnight, and then incubated for 1 h at room temperature, respectively, with dinoflagellate Form II RuBisCO antiserum ( ) at 2500-fold dilution in TBST and GAPDH antiserum (Cat NO. D261392, BBI Life Science, Sangon Biotech, Shanghai, China) at 2500-fold dilution in TBST. ..

Article Title: Effect and mechanism of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain and spinal microglia in a rat model of chronic constriction injury
Article Snippet: .. Next to the incubation in the primary antibody was TBST (short for Tris-HCl buffer with Tween 20) rinse (5 minutes × 3 times) and then incubated (room temperature, 1 hour) with the horseradish peroxidase (HRP)-labeled secondary goat-anti-rabbit (1:5000; Shanghai Sangon Biological Engineering Technology and Services Co, Ltd, Shanghai, China). .. After that, the PVDF membrane was rinsed (5 min × 3 times) for development in chemiluminescent agent on a Bio-rad GEL DOC EZ (Bio-rad, California, USA).

Article Title: Molecular Characterization, Phylogenetic, Expression, and Protective Immunity Analysis of OmpF, a Promising Candidate Immunogen Against Yersinia ruckeri Infection in Channel Catfish
Article Snippet: .. After washing three times with TBST, the membranes were incubated with goat-anti-rabbit IgG-HRP (1:5000, Sangon Biotech) for 1h at 37°C. .. The reaction was visualized using DAB (Sigma) for 5 to 15 min, and terminated by rinsing with distilled water.

other:

Article Title: YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization
Article Snippet: After being washed in TBST 3 times, membranes were probed with corresponding secondary antibodies (Sangon Biotech, D110058/D110087/D110117) for 1 h at 25°C.

Article Title: DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma
Article Snippet: The PVDF membranes were blocked with 5% skim milk in TBST (Sangon Biotech, Shanghai, China) for 1 hour at room temperature (RT).

Article Title: Construction of MC1R and ASIP Eukaryotic Expression Vector and its Regulation of Plumage Color in Japanese Quail (Coturnix japonica)
Article Snippet: After washing 5 times with 1× TBST, the proteins of MCIR, ASIP, and β -actin were detected on the membrane with the ultra-sensitive horseradish from the catalase DAB color kit (Sangon Biotech, China).

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    Sangon Biotech tbst
    Tbst, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Sangon Biotech
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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