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GE Healthcare tbst
Tbst, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/GE Healthcare
Average 99 stars, based on 3540 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
99/100 stars

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Incubation:

Article Title: Immobilization contributes to exaggerated neuropeptide signaling, inflammatory changes, and nociceptive sensitization after fracture in rats
Article Snippet: .. After washing in TBST three times, the blot was then incubated in ECL plus chemoluminescence reagents (Amersham) and scanned by PhosphoImager (Typhoon, GE Healthcare) and the band intensity was analyzed using ImageQuant 5.2 software (Molecular Dynamics) and normalized with the corresponding internal loading control band, β-actin. ..

Article Title: Connexin45-containing neuronal gap junctions in rodent retina also contain connexin36 in both apposing hemiplaques, forming bi-homotypic gap junctions, with scaffolding contributed by zonula occludens-1
Article Snippet: .. Membranes were washed in TBSTw for 40 min, incubated with horseradish peroxidase-conjugated donkey anti-rabbit IgG or anti-mouse IgG diluted 1:3000-1:5000 (Sigma-Aldrich Canada) in TBSTw containing 1% non-fat milk powder, washed in TBST for 40 min and resolved by chemiluminescence (ECL, Amersham PB, Baie d'Urfe, Quebec, Canada). .. Mouse retinas and HeLa cell lysates were homogenized in IP buffer, sonicated and centrifuged at 20,000 x g for 10 min at 4°C.

Article Title: Cleavage and Sub-Cellular Redistribution of Nuclear Pore Protein 98 by Coxsackievirus B3 Protease 2A Impairs Cardioprotection
Article Snippet: .. Membranes were subsequently washed in TBST three times for 10 min each, followed by incubation with the appropriate secondary antibody (goat anti-mouse or donkey anti-rabbit) conjugated to horseradish peroxidase (Amersham) and visualized using the enhanced chemiluminescence method as per the manufacturer's instructions (Amersham). .. Protein expression was quantified by densitometry using the NIH ImageJ software package ( http://imagej.nih.gov/ij/index.html ) and normalized to GAPDH or β-actin as indicated based upon an n of 3 for each experiment and depicted graphically next to each blot in the figure.

Article Title: Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Is a Coregulator of K-Rta: Physical Association and Promoter-Dependent Transcriptional Repression
Article Snippet: .. After incubation with the secondary antibodies, the membranes were washed three times with TBST and visualized with enhanced chemiluminescence reagents (Amersham Pharmacia). ..

Article Title: The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila
Article Snippet: .. Blots were washed in TBST and then incubated with ECL reagent (GE Healthcare) and exposed with Hyperfilm ECL (GE Healthcare). .. Online supplemental material Fig. S1 displays the localization of early endosomal markers in wild-type and Vps34 mutant cells.

Article Title: Selective manipulation of the inositol metabolic pathway for induction of salt-tolerance in indica rice variety
Article Snippet: .. Then the membrane was immuno-probed with a secondary antibody raised as goat anti-rabbit at 1:5000 (v/v) dilution in TBST [1XTBS and 0.1% (v/v) Tween 20] for 2 hrs. at room temperature, washed with large volumes of TBST 2 times with fresh changes of wash buffer every 10 min and incubated with horseradish peroxidase (HRP)-linked luminescence in dark for few minutes and developed with a florescent ECL detection kit reagent (Amersham Pharmacia Biotech, Little Chalfont, Bucks, UK) following manufacturer’s instruction. ..

Imaging:

Article Title: Hybrid topoisomerase I and HDAC inhibitors as dual action anticancer agents
Article Snippet: .. After three washes with TBST the blots were developed with ECL substrate (GE Healthcare) and chemiluminescence detected by ChemiDoc Imaging System (Biorad). .. The optical density of the protein bands detected by western blotting was normalized on α -tubulin levels and analyzed by ChemiDoc software (Biorad).

Software:

Article Title: Immobilization contributes to exaggerated neuropeptide signaling, inflammatory changes, and nociceptive sensitization after fracture in rats
Article Snippet: .. After washing in TBST three times, the blot was then incubated in ECL plus chemoluminescence reagents (Amersham) and scanned by PhosphoImager (Typhoon, GE Healthcare) and the band intensity was analyzed using ImageQuant 5.2 software (Molecular Dynamics) and normalized with the corresponding internal loading control band, β-actin. ..

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  • 99
    GE Healthcare tbst
    Tbst, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/GE Healthcare
    Average 99 stars, based on 3540 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare tween tbst
    Tween Tbst, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween tbst/product/GE Healthcare
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    tween tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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