Structured Review

Fisher Scientific tbst
Tbst, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Fisher Scientific
Average 94 stars, based on 43 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
94/100 stars

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Diagnostic Assay:

Article Title: Neuroprotective Effect of the Marine-Derived Compound 11-Dehydrosinulariolide through DJ-1-Related Pathway in In Vitro and In Vivo Models of Parkinson’s Disease
Article Snippet: .. Cultures were rinsed again with TBST as before, mounted on glass slides (Fisher Scientific, Waltham, MA, USA) with SlowFade Light (Molecular Probes, Eugene, OR, USA), and visualized using Leica Optical’s Confocal Laser Scanning Microscopy System (Leica, DM6000B) with the SPOT program (Diagnostic, RT slider SPOT, Hamilton, CA, USA). ..

Electrophoresis:

Article Title: 6-Bromoindirubin-3′oxime (BIO) decreases proliferation and migration of canine melanoma cell lines
Article Snippet: .. Protein lysates (approximately 50 µg) were separated by electrophoresis on a 7.5% SDS polyacrylamide gel (BioRad) at 150 V for approximately 45 min. Proteins were then transferred onto a nitrocellulose membrane (Whatman) at 100 V for 1 h, then blocked with TBST containing 5% non-fat dry milk and 1% bovine serum albumin for 1 h (all reagents from Fisher Scientific). .. The membranes were probed overnight at 4 °C with either a rabbit polyclonal anti-Axin2 (Conductin) antibody (sc-20784, Santa Cruz Biotechnology) diluted 1:200 in blocking solution or a goat polyclonal anti-actin (sc-1615, Santa Cruz Biotechnology) diluted 1:200 in blocking solution.

Incubation:

Article Title: Insulin-Like Growth Factor-1 Preconditioning Accentuates Intrinsic Survival Mechanism in Stem Cells to Resist Ischemic Injury by Orchestrating Protein Kinase C?-Erk1/2 Activation
Article Snippet: .. After washing with TBST, membranes were incubated with ECL or Femto supersensitive detection reagent, and signals were exposed to Kodak Light film (Fisher Scientific). .. TUNEL was performed on paraformaldehyde fixed cells with an in situ cell death detection kit (TMR Red; Roche Applied Science) according to manufacturer's instructions.

Article Title: MicroRNA miR-128 represses LINE-1 (L1) retrotransposition by down-regulating the nuclear import factor TNPO1
Article Snippet: .. After developing, each blot was washed three times in 1× TBST (TBS (BP24711, FisherSci) and 0.05% Tween 20 (BP337-500, FisherSci)) prior to incubation with the next primary antibody. .. Immunopurification of Argonaute from HeLa cell extracts was performed using the 4F9 antibody (Santa Cruz Biotechnology) as described previously ( , ).

Article Title: An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progression
Article Snippet: .. Membranes were washed again three times with TBST (10 min/wash) and then incubated with Luminata Forte Western HRP Substrate (Fisher Scientific) for two minutes before imaging with ChemiDoc (BioRad). .. Densitometry was performed with ImageLab v4.0.1 (BioRad).

Article Title: Pentose Phosphate Shunt Modulates Reactive Oxygen Species and Nitric Oxide Production Controlling Trypanosoma cruzi in Macrophages
Article Snippet: .. The membranes were washed three times with TBST (TBS + 0.1% Tween-20; Fisher Scientific), and incubated for 1 h at room temperature with HRP-conjugated anti-mouse antibody (Southern Biotech, Birmingham, AL, USA, 1: 10,000 dilution in 3% BSA). .. Proteins with antibody conjugation were detected by using the chemiluminescence based method (GE Healthcare, Chicago, IL, USA), and imaged and analyzed by using an Image Quant LAS4000 system (GE Healthcare) ( ).

Confocal Laser Scanning Microscopy:

Article Title: Neuroprotective Effect of the Marine-Derived Compound 11-Dehydrosinulariolide through DJ-1-Related Pathway in In Vitro and In Vivo Models of Parkinson’s Disease
Article Snippet: .. Cultures were rinsed again with TBST as before, mounted on glass slides (Fisher Scientific, Waltham, MA, USA) with SlowFade Light (Molecular Probes, Eugene, OR, USA), and visualized using Leica Optical’s Confocal Laser Scanning Microscopy System (Leica, DM6000B) with the SPOT program (Diagnostic, RT slider SPOT, Hamilton, CA, USA). ..

Imaging:

Article Title: An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progression
Article Snippet: .. Membranes were washed again three times with TBST (10 min/wash) and then incubated with Luminata Forte Western HRP Substrate (Fisher Scientific) for two minutes before imaging with ChemiDoc (BioRad). .. Densitometry was performed with ImageLab v4.0.1 (BioRad).

Western Blot:

Article Title: An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progression
Article Snippet: .. Membranes were washed again three times with TBST (10 min/wash) and then incubated with Luminata Forte Western HRP Substrate (Fisher Scientific) for two minutes before imaging with ChemiDoc (BioRad). .. Densitometry was performed with ImageLab v4.0.1 (BioRad).

Sonication:

Article Title: Algal chloroplast produced camelid VHH antitoxins are capable of neutralizing botulinum neurotoxin
Article Snippet: .. Approximately one loop full of cells were resuspended in 500 μL TBST and sonicated on ice for two 10-second intervals at 25% amplitude (Fisher Scientific Sonic Dismembrator Model 500). .. Soluble protein was separated from the lysates by centrifugation in a tabletop centrifuge at 20,000 × g for 30 minutes at 4° C. SDS-PAGE was performed using Bio-Rad mini-protean TGX 10% gels and the gels were analyzed by immunoblot against the Flag tag.

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    Fisher Scientific tbst
    Tbst, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Fisher Scientific
    Average 94 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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