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FUJIFILM tbst
Tbst, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/FUJIFILM
Average 93 stars, based on 16 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
93/100 stars

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Related Articles

Immunohistochemistry:

Article Title: Investigating the neuroprotective effect of AAV-mediated β-synuclein overexpression in a transgenic model of synucleinopathy
Article Snippet: .. For the detection phosphorylated at S129 α-syn we used a rabbit antibody (ref: ab51253, Abcam, Cambridge, GB) diluted at 1:300 in TBST for immunohistochemistry, and a mouse antibody (ref: pSyn#64, FUJIFILM Wako Pure Chemical Corporation, China) diluted at 1:1000 in TBST for immunofluorescence analysis (with the rabbit anti-β-syn antibody, Supplementary Fig. ), at 4 °C overnight. .. After another blocking step 30 minutes at RT, for immunohistochemistry, sections were incubated 1 h at RT with anti-rabbit IgG HRP conjugate (ref. 4010-05, SouthernBiotech, Birmingham, AL, USA) diluted at 1:250 in PBST or TBST.

Labeling:

Article Title: Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing, Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing
Article Snippet: .. The membranes were washed three times for 15 min with TBST (Tris‐buffered saline, 0.1% Tween 20) and incubated for 1 h at room temperature in a blocking solution with horseradish peroxidase tagged second antibodies the labeled proteins were visualized with LAS‐3000 Luminescent Image Analyzer (FujiFilm, Tokyo, Japan). .. The antibodies used in this study are as follows: CD86 (abcam, ab112490, 1:200), iNOS (abcam, ab210823, 1:200), Arginase (CST, #93668, 1:200), CD206 (Santa Cruz Biotechnology, SC‐376108, 1:200), β‐actin (abcam, ab8227, 1:1000), CD63 (Santa Cruz Biotechnology, SC‐15363, 1:200), Alix (abcam, ab117600, 1:200), MMP2 (CST, #4022, 1:200), and VEGF (Santa Cruz Biotechnology, SC‐7269, 1:200).

Incubation:

Article Title: Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing, Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing
Article Snippet: .. The membranes were washed three times for 15 min with TBST (Tris‐buffered saline, 0.1% Tween 20) and incubated for 1 h at room temperature in a blocking solution with horseradish peroxidase tagged second antibodies the labeled proteins were visualized with LAS‐3000 Luminescent Image Analyzer (FujiFilm, Tokyo, Japan). .. The antibodies used in this study are as follows: CD86 (abcam, ab112490, 1:200), iNOS (abcam, ab210823, 1:200), Arginase (CST, #93668, 1:200), CD206 (Santa Cruz Biotechnology, SC‐376108, 1:200), β‐actin (abcam, ab8227, 1:1000), CD63 (Santa Cruz Biotechnology, SC‐15363, 1:200), Alix (abcam, ab117600, 1:200), MMP2 (CST, #4022, 1:200), and VEGF (Santa Cruz Biotechnology, SC‐7269, 1:200).

Article Title: Robust amyloid clearance in a mouse model of AD provides novel insights into the mechanism of A? immunotherapy
Article Snippet: .. Sections were rinsed, treated with 0.9% hydrogen peroxide in TBST (TBS + 0.02% Triton-X), and blocked with TBST plus 1.5% goat serum before overnight incubation at room temperature with primary antibody diluted in TBST (Wako Rb anti-Iba1 #019-19741, 1:15,000 or Serotec biotinylated Rt anti-CD68 #MCA1957BT, 1:400). .. Binding was detected with biotinconjugated secondary antibodies followed by HRP-avidin and developed with DAB.

Article Title: Metabolic profiling of isolated mitochondria and cytoplasm reveals compartment-specific metabolic responses
Article Snippet: .. After three 5 min washing steps in TBST, membranes were incubated in ECL solution (Haan and Behrmann ) and chemiluminescence signals were detected by a LAS-4000 camera system (Fujifilm). ..

Blocking Assay:

Article Title: Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing, Exosome‐Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing
Article Snippet: .. The membranes were washed three times for 15 min with TBST (Tris‐buffered saline, 0.1% Tween 20) and incubated for 1 h at room temperature in a blocking solution with horseradish peroxidase tagged second antibodies the labeled proteins were visualized with LAS‐3000 Luminescent Image Analyzer (FujiFilm, Tokyo, Japan). .. The antibodies used in this study are as follows: CD86 (abcam, ab112490, 1:200), iNOS (abcam, ab210823, 1:200), Arginase (CST, #93668, 1:200), CD206 (Santa Cruz Biotechnology, SC‐376108, 1:200), β‐actin (abcam, ab8227, 1:1000), CD63 (Santa Cruz Biotechnology, SC‐15363, 1:200), Alix (abcam, ab117600, 1:200), MMP2 (CST, #4022, 1:200), and VEGF (Santa Cruz Biotechnology, SC‐7269, 1:200).

Western Blot:

Article Title: Combined treatment with dipeptidyl peptidase 4 (DPP4) inhibitor sitagliptin and elemental diets reduced indomethacin-induced intestinal injury in rats via the increase of mucosal glucagon-like peptide-2 concentration
Article Snippet: .. Blots were washed 3 times with TBST and developed using an enhanced chemiluminescence system (ECL Prime Western Blotting Detection System, Buckinghamshire, UK, and FUJIFILM Imaging System Application Note LAS-3000, FUJIFILM, Tokyo, Japan). ..

Immunofluorescence:

Article Title: Investigating the neuroprotective effect of AAV-mediated β-synuclein overexpression in a transgenic model of synucleinopathy
Article Snippet: .. For the detection phosphorylated at S129 α-syn we used a rabbit antibody (ref: ab51253, Abcam, Cambridge, GB) diluted at 1:300 in TBST for immunohistochemistry, and a mouse antibody (ref: pSyn#64, FUJIFILM Wako Pure Chemical Corporation, China) diluted at 1:1000 in TBST for immunofluorescence analysis (with the rabbit anti-β-syn antibody, Supplementary Fig. ), at 4 °C overnight. .. After another blocking step 30 minutes at RT, for immunohistochemistry, sections were incubated 1 h at RT with anti-rabbit IgG HRP conjugate (ref. 4010-05, SouthernBiotech, Birmingham, AL, USA) diluted at 1:250 in PBST or TBST.

Imaging:

Article Title: Combined treatment with dipeptidyl peptidase 4 (DPP4) inhibitor sitagliptin and elemental diets reduced indomethacin-induced intestinal injury in rats via the increase of mucosal glucagon-like peptide-2 concentration
Article Snippet: .. Blots were washed 3 times with TBST and developed using an enhanced chemiluminescence system (ECL Prime Western Blotting Detection System, Buckinghamshire, UK, and FUJIFILM Imaging System Application Note LAS-3000, FUJIFILM, Tokyo, Japan). ..

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    FUJIFILM tbst
    Tbst, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/FUJIFILM
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    93/100 stars
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