Structured Review

Cappel Laboratories tbst
Tbst, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Cappel Laboratories
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Incubation:

Article Title: Loss of Matrix Adhesion Triggers Rapid Transformation-Selective Apoptosis in Fibroblasts
Article Snippet: .. 800 ng of recombinant PARP was added to 40 μl of 10,000 g supernatant and incubated at 37°C for 1 h. Samples were resolved on SDS-PAGE minigels, transferred to nitrocellulose for 40 min at 50 V, blocked overnight at 4°C in 150 mM NaCl, 50 mM Tris (pH 8.0), and 5% powdered milk, washed with Tris-buffered saline and Tween 20 (TBST), incubated with C2-10 αPARP monoclonal antibody (gift of Guy Poirier Université Lavat, Quebec, Canada) 1:10,000 in TBST for 2 h at room temperature, washed with TBST, and finally incubated with Peroxidase-conjugated goat anti–mouse antibody 1:10,000 in TBST (Cappel Laboratories, Cochranville, PA). .. ECL (Renaissance; Dupont NEN , Boston, MA) was then performed for 1 min at room temperature according to the manufacturer's recommendations.

Recombinant:

Article Title: Loss of Matrix Adhesion Triggers Rapid Transformation-Selective Apoptosis in Fibroblasts
Article Snippet: .. 800 ng of recombinant PARP was added to 40 μl of 10,000 g supernatant and incubated at 37°C for 1 h. Samples were resolved on SDS-PAGE minigels, transferred to nitrocellulose for 40 min at 50 V, blocked overnight at 4°C in 150 mM NaCl, 50 mM Tris (pH 8.0), and 5% powdered milk, washed with Tris-buffered saline and Tween 20 (TBST), incubated with C2-10 αPARP monoclonal antibody (gift of Guy Poirier Université Lavat, Quebec, Canada) 1:10,000 in TBST for 2 h at room temperature, washed with TBST, and finally incubated with Peroxidase-conjugated goat anti–mouse antibody 1:10,000 in TBST (Cappel Laboratories, Cochranville, PA). .. ECL (Renaissance; Dupont NEN , Boston, MA) was then performed for 1 min at room temperature according to the manufacturer's recommendations.

SDS Page:

Article Title: Loss of Matrix Adhesion Triggers Rapid Transformation-Selective Apoptosis in Fibroblasts
Article Snippet: .. 800 ng of recombinant PARP was added to 40 μl of 10,000 g supernatant and incubated at 37°C for 1 h. Samples were resolved on SDS-PAGE minigels, transferred to nitrocellulose for 40 min at 50 V, blocked overnight at 4°C in 150 mM NaCl, 50 mM Tris (pH 8.0), and 5% powdered milk, washed with Tris-buffered saline and Tween 20 (TBST), incubated with C2-10 αPARP monoclonal antibody (gift of Guy Poirier Université Lavat, Quebec, Canada) 1:10,000 in TBST for 2 h at room temperature, washed with TBST, and finally incubated with Peroxidase-conjugated goat anti–mouse antibody 1:10,000 in TBST (Cappel Laboratories, Cochranville, PA). .. ECL (Renaissance; Dupont NEN , Boston, MA) was then performed for 1 min at room temperature according to the manufacturer's recommendations.

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    Cappel Laboratories tbst
    Tbst, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Cappel Laboratories
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
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