Structured Review

Boston BioProducts tbst
Tbst, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Boston BioProducts
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
92/100 stars

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Incubation:

Article Title: A multiplexed system for quantitative comparisons of chromatin landscapes
Article Snippet: .. Transferred nitrocellulose membrane was incubated with 5% BSA (Sigma A2153-50g) in TBST (Boston Bioproducts IBB-181), and blotted with respective antibodies overnight. ..

Article Title: A novel method for the isolation of extracellular vesicles and RNA from urine
Article Snippet: .. The blots were blocked with 5% milk in TBST (tris buffered saline-tween 20; Boston Bioproducts, Ashland, Massachusetts, USA) for 1 h at RT, and incubated with rabbit anti-CD9 (1:1000; Cell Signaling Technologies, Danvers, Massachusetts, USA), rabbit anti-Rab5 (1:300; Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-CD63 (1:200, Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-aquaporin-2 (1:1000; Abcam, Cambridge, Massachusetts, USA), and rabbit anti-Hsp70 (1:1000; Abcam) antibodies in blocking buffer overnight at 4°C. .. After incubation, the blots were washed with TBST and incubated for 1 h at RT with goat anti-rabbit HRP antibody (1:2000; Cell Signaling Technologies) in blocking buffer.

Stripping Membranes:

Article Title: Internalized gap junctions are degraded by autophagy
Article Snippet: .. Membranes were washed in TBST for 15 min, stripped in stripping buffer (Boston Bioproducts, BP-96) at 65°C for 30 min, followed by a stringent wash in TBST. .. Membranes were re-blocked with 5% dry milk in TBST and re-probed with mouse monoclonal antibodies directed against α-tubulin (Sigma, T9026) or β-tubulin (Developmental Studies Hybridoma Bank, clone E7) (1:5000 in 5% BSA in PBS) to serve as a loading control.

Blocking Assay:

Article Title: A novel method for the isolation of extracellular vesicles and RNA from urine
Article Snippet: .. The blots were blocked with 5% milk in TBST (tris buffered saline-tween 20; Boston Bioproducts, Ashland, Massachusetts, USA) for 1 h at RT, and incubated with rabbit anti-CD9 (1:1000; Cell Signaling Technologies, Danvers, Massachusetts, USA), rabbit anti-Rab5 (1:300; Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-CD63 (1:200, Santa Cruz Biotechnology, Dallas, Texas, USA), rabbit anti-aquaporin-2 (1:1000; Abcam, Cambridge, Massachusetts, USA), and rabbit anti-Hsp70 (1:1000; Abcam) antibodies in blocking buffer overnight at 4°C. .. After incubation, the blots were washed with TBST and incubated for 1 h at RT with goat anti-rabbit HRP antibody (1:2000; Cell Signaling Technologies) in blocking buffer.

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    Boston BioProducts 1x tbst
    1x Tbst, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x tbst/product/Boston BioProducts
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x tbst - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Boston BioProducts tbst buffer
    Tbst Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst buffer/product/Boston BioProducts
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbst buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Boston BioProducts tween 20 tbst buffer
    Tween 20 Tbst Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 tbst buffer/product/Boston BioProducts
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tween 20 tbst buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Boston BioProducts tbst
    Tbst, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Boston BioProducts
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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