Structured Review

Beijing Solarbio Science tbst
Tbst, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Beijing Solarbio Science
Average 94 stars, based on 16 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
94/100 stars

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Related Articles

Western Blot:

Article Title: Gambogenic acid exerts anticancer effects in cisplatin-resistant non-small cell lung cancer cells
Article Snippet: .. Following washing in TBST three times, immunoblots were visualized using an enhanced chemiluminescent substrate kit (Beijing Solarbio Science & Technology Co., Ltd.) under an Amersham Imager 600 system (GE Healthcare Life Sciences). .. Expression levels were quantified using ImageJ version 1.52 software (National Institutes of Health).

Article Title: Hydrogen-rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus-activated kinase 2/signal transducer and activator of transcription 3 signaling pathway
Article Snippet: .. Following four washes with TBST [Beijing Solarbio Science & Technology Co., Ltd.) with 20% Tween-20 (cat. no. T8220; Beijing Solarbio Science & Technology Co., Ltd.)], the membranes were developed with an enhanced chemiluminescence kit (Pierce™ ECL Plus Western Blotting Substrate; cat no. 32132; Thermo Fisher Scientific, Inc,) and visualized with a Gel Doc™ EZ System (cat. no. 1708270; Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

Incubation:

Article Title: Inactivated Tianjin strain, a novel genotype of Sendai virus, induces apoptosis in HeLa, NCI-H446 and Hep3B cells
Article Snippet: .. The membranes were then incubated with anti-mouse IgG HRP-linked antibody or anti-rabbit IgG HRP-linked antibody for 1 h at room temperature and washed 3 times for 5 min each in TBST, and the protein levels were measured using a chemiluminescence kit (Beijing Solarbio Science & Technology Co., Ltd.). .. Protein signals were analyzed by ImageJ version 1.43 software (National Institutes of Health, Bethesda, MA, USA). β-actin served as the internal control.

Article Title: Effect of haptoglobin on the treatment of chronic hepatitis B with interferon
Article Snippet: .. The membranes were blocked at room temperature for 1 h with 5% non-fat milk in Tris-buffered saline solution with Tween-20 (TBS-T) and incubated overnight with the primary antibodies (all 1:500) in TBST plus 5% bovine serum albumin (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) at 4°C. .. After washing three times with TBS-T buffer for 5 min, the membranes were incubated with a horseradish peroxidase-conjugated goat anti-rabbit IgG as the secondary antibody (1:5,000) for 1 h at room temperature.

Article Title: Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model
Article Snippet: .. The membranes were blocked in TBST containing 0.1% Tween-20 (Solarbio Life Sciences, China) and 5% non-fat dry milk for 2 h at room temperature, and incubated with antibody to c-Kit (in 1:500 dilution SC-168, Santa Cruz Biotechnology, USA) and β-actin (in 1:2000 dilution, Cell Signaling Technology, USA) overnight at 4°C. .. Then, membranes were washed with TBST followed by 30 min incubation with horseradish peroxidase-conjugated secondary antibody (1:2000 dilution; Proteintech, China) at room temperature and detected using enhanced chemiluminescence (Thermofisher Scientific, USA).

Article Title: Sufentanil impairs autophagic degradation and inhibits cell migration in NCI-H460 in vitro
Article Snippet: .. The primary antibodies incubated membranes were washed 5 times with TBST (Beijing Solarbio Science & Technology Co., Ltd.) and then incubated with relevant secondary antibodies for 1 h at 37°C. .. Eventually, bands were detected using enhanced chemiluminescence and visualized using a chemiluminescence imaging instrument (GE Image Quant LAS 4000; GE Healthcare Life Sciences).

other:

Article Title: Identification of a protein associated with the activity of cytokine-induced killer cells
Article Snippet: Corp., Luoyang, China) at a dilution of 1:1,000 in TBST containing 5% skim milk at room temperature for 40 min. Next, the membrane was washed two times with TBS for 20 min and analyzed using the ECL Plus (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) chemiluminescence detection method.

Article Title: Exploring the Antioxidant Effects and Periodic Regulation of Cancer Cells by Polyphenols Produced by the Fermentation of Grape Skin by Lactobacillus plantarum KFY02
Article Snippet: After washing with 1× TBST (Solarbio Life Sciences, Beijing, China) five times, the blots were treated with a second antibody (Cell Signaling Technology Inc., Danvers, MA, USA) for 1 h and washed with 1× TBST five times.

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  • 94
    Beijing Solarbio Science tbst
    Tbst, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Beijing Solarbio Science
    Average 94 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    92
    Beijing Solarbio Science tbst buffer
    Tbst Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst buffer/product/Beijing Solarbio Science
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tbst buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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