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Beijing CWBio tbst
Tbst, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Beijing CWBio
Average 94 stars, based on 98 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-08
94/100 stars

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Related Articles

Western Blot:

Article Title: Hemopexin alleviates cognitive dysfunction after focal cerebral ischemia-reperfusion injury in rats
Article Snippet: .. The membranes were washed in TBST (Tris-buffered saline Tween-20) 3 times (5 min each time), followed by incubation with the corresponding goat anti-rabbit secondary antibodies (11,000 dilution, CWBIO, Peking, China) to recognize the bound antibodies at room temperature for 2 h. The immunoblots were immersed in enhanced chemiluminescent (ECL) reagent to visualize the specific protein bands, followed by exposure to ECL-hyperfilm (Amersham Biosciences, Piscataway, NJ, USA). .. Immunofluorescence staining Rats were sacrificed after being intraperitoneally anesthetized at 7 days after reperfusion.

Article Title: Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)
Article Snippet: .. For western blot, separated proteins were electrotransferred to a PVDF membrane (Millipore), and the membrane was subsequently blocked with 5% skim milk powder in TBST for 1 h. Target proteins were verified with mouse anti-His mouse monoclonal antibodies (CWBIO) followed by staining with goat-anti-mouse IgG (CWBIO). .. Blots were visualized using WesternBright ECL kit (Advasta), and signal was detected using a chemiluminescence imaging system (Clinx Science Instruments, Shanghai, China).

Incubation:

Article Title: TRP-2 mediates coat color pigmentation in sheep skin
Article Snippet: .. Horse radish peroxidase-conjugated anti-mouse IgG (1:10,000; cat. no. BA1001; Wuhan Boster Biological Technology, Ltd.) was added to cover the membrane and incubated at 37°C with horizontal shaking for 1 h. Following washing six times with TBST for 5 min each, the membrane was developed using the highly sensitive, enhanced chemiluminescence solution (cat. no. CW00495; CW Biotech) consisting of solutions A and B at 1:1 ratio followed by film exposure. .. The TRP-2, MITF and GAPDH immunoblot results were analyzed using the Image-ProPlus version 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA) to measure the area and gray value of each target band.

Article Title: Mitochondrial Pyruvate Carriers Prevent Cadmium Toxicity by Sustaining the TCA Cycle and Glutathione Synthesis 1Mitochondrial Pyruvate Carriers Prevent Cadmium Toxicity by Sustaining the TCA Cycle and Glutathione Synthesis 1 [OPEN]
Article Snippet: .. The membranes were washed with TBST (20 m m Tris-Cl, 150 m m NaCl, and 0.05% [v/v] Tween 20), blocked with TBST containing 5% (w/v) nonfat milk (TBSTM) for 2 h, and incubated with anti-FLAG antibody (1:5,000; catalog no. CW0287M; CWBio) or anti-C-MYC antibody (1:5,000; catalog no. CW0259M; CWBio) in TBSTM for 5 h at room temperature. .. The membranes were washed four times (5 min each) and then incubated with goat anti-mouse IgG (catalog no. CW0110s; CWBio) in fresh TBSTM for about 2 h. After the membranes were washed six times with TBST, the bound antibodies were monitored with the enhanced chemiluminescence substrate (catalog no. 32132; Thermo).

Article Title: Bavachinin Induces Oxidative Damage in HepaRG Cells through p38/JNK MAPK Pathways
Article Snippet: .. The membranes were subsequently washed with TBST and incubated with the appropriate secondary antibody at room temperature for 2 h. Following washes with TBST, the protein bands were visualized using an ECL system (CW Biotech, Beijing, China) and imaged using a Bio-Rad imaging system (Bio-Rad, Hercules, CA, USA). .. Cell Viability Cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium, Amresco, 0973) assay as previously described [ ].

Article Title: Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice
Article Snippet: .. Following three washes in TBST, the sections were incubated with goat anti-mouse IgG-HRP (Cat. CW0102 CWBIO, Beijing, China), diluted 1/200 in 1% w/v skimmed milk, for 1 h at room temperature. .. Following three washes with TBST, the sections were treated with 3,3′-diaminobenzidine solution before observation by light microscopy.

Article Title: Hemopexin alleviates cognitive dysfunction after focal cerebral ischemia-reperfusion injury in rats
Article Snippet: .. The membranes were washed in TBST (Tris-buffered saline Tween-20) 3 times (5 min each time), followed by incubation with the corresponding goat anti-rabbit secondary antibodies (11,000 dilution, CWBIO, Peking, China) to recognize the bound antibodies at room temperature for 2 h. The immunoblots were immersed in enhanced chemiluminescent (ECL) reagent to visualize the specific protein bands, followed by exposure to ECL-hyperfilm (Amersham Biosciences, Piscataway, NJ, USA). .. Immunofluorescence staining Rats were sacrificed after being intraperitoneally anesthetized at 7 days after reperfusion.

Article Title: AIM2 Mediates Inflammation-Associated Renal Damage in Hepatitis B Virus-Associated Glomerulonephritis by Regulating Caspase-1, IL-1β, and IL-18
Article Snippet: .. After washing in TBST, the membranes were incubated with horseradish peroxidase- (HRP-) conjugated secondary antibody (CWBio, Beijing, China) diluted 1 : 10,000 in TBST and incubated for 1 h at room temperature. .. After washing extensively in TBST, membranes were immersed in ECL detection reagent (Beyotime Institute Biotechnology, Nantong, China) followed by exposure to ChemiDoc XRS + system (Bio-Rad, California, USA).

Article Title: Screening common signaling pathways associated with drug resistance in non‐small cell lung cancer via gene expression profile analysis, et al. Screening common signaling pathways associated with drug resistance in non‐small cell lung cancer via gene expression profile analysis
Article Snippet: .. Following incubation, the membranes were washed thrice with TBST and treated with an enhanced chemiluminescence (ECL) substrate (CWBIO, Beijing, China, #CW00495). .. FluorChem E System (Protein Simple, USA) was used to evaluate protein expression.

Imaging:

Article Title: Bavachinin Induces Oxidative Damage in HepaRG Cells through p38/JNK MAPK Pathways
Article Snippet: .. The membranes were subsequently washed with TBST and incubated with the appropriate secondary antibody at room temperature for 2 h. Following washes with TBST, the protein bands were visualized using an ECL system (CW Biotech, Beijing, China) and imaged using a Bio-Rad imaging system (Bio-Rad, Hercules, CA, USA). .. Cell Viability Cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium, Amresco, 0973) assay as previously described [ ].

Staining:

Article Title: Functional Analysis of a Carboxylesterase Gene Associated With Isoprocarb and Cyhalothrin Resistance in Rhopalosiphum padi (L.)
Article Snippet: .. For western blot, separated proteins were electrotransferred to a PVDF membrane (Millipore), and the membrane was subsequently blocked with 5% skim milk powder in TBST for 1 h. Target proteins were verified with mouse anti-His mouse monoclonal antibodies (CWBIO) followed by staining with goat-anti-mouse IgG (CWBIO). .. Blots were visualized using WesternBright ECL kit (Advasta), and signal was detected using a chemiluminescence imaging system (Clinx Science Instruments, Shanghai, China).

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    Beijing CWBio tbst
    Tbst, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Beijing CWBio
    Average 94 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Beijing CWBio tween 20 tbst
    Tween 20 Tbst, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 tbst/product/Beijing CWBio
    Average 93 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    tween 20 tbst - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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