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Becton Dickinson tbst
Tbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Becton Dickinson
Average 94 stars, based on 89 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
94/100 stars

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Incubation:

Article Title: SOD and inhaled nitric oxide normalize phosphodiesterase 5 expression and activity in neonatal lambs with persistent pulmonary hypertension
Article Snippet: .. Briefly, membranes were blocked for 1 h at room temperature with 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 (1× TBST) and incubated overnight at 4°C with the primary antibody in 5% milk + 1× TBST at an appropriate dilution [1:500 for mouse anti-PDE5 (BD Transduction Laboratories, San Jose, CA), 1:500 for rabbit anti-sGCβ (BD Transduction Laboratories), and 1:2,000 for mouse β-actin (Sigma)]. .. The membranes were washed and incubated with the appropriate secondary antibody conjugated to horseradish peroxidase (Pierce, Rockford, IL) diluted 1:1,000 in 5% milk + 1× TBST.

Article Title: Plectin-targeted liposomes enhance the therapeutic efficacy of a PARP inhibitor in the treatment of ovarian cancer
Article Snippet: .. The slides were washed three times in TBST, followed by incubation with HRP-conjugated goat anti-rabbit secondary antibody (BD Biosciences, San Jose, CA). ..

Article Title: Integration of Kinase and Calcium Signaling at the Level of Chromatin Underlies Inducible Gene Activation in T Cells
Article Snippet: .. The blots were then washed in TBST and incubated overnight with anti-NFATc2 (NFAT1) (BD Transduction Laboratories) or anti–phospho-p65 (S536) (Cell Signaling Technology) primary Abs (BD Transduction Laboratories) at 1:5000 and 1:1000 dilution, respectively, in 5% (w/v) BSA in TBST. .. Blots were washed in TBST and then incubated for a further hour with a 1:2000 dilution of HRP-conjugated anti-IgG (Cell Signaling Technology).

Article Title: Efficacy and Safety of A Liposome-Based Vaccine against Protein Tau, Assessed in Tau.P301L Mice That Model Tauopathy
Article Snippet: .. Non-specific binding to membranes was blocked by incubation in 5% non-fat dry milk in TBST (0.1% Tween-20, 50 mM Tris.HCl, pH 7.6, 150 mM NaCl) prior to overnight incubation at 4°C with primary antibody as indicated: Tau5 (anti-pan Tau; BD bioscience, USA), HT7 (anti-human Tau; Thermo Scientific, USA), pS396 (Invitrogen, UK), AT180 (anti-pT231, Innogenetics, Belgium), pS404 (Sigma, USA) and GFAP (DAKO, Denmark) all diluted in the same blocking buffer. .. After washing in TBST, blots were incubated for 1 hour at RT with secondary goat anti-mouse or anti-rabbit, HRP-conjugated (DAKO, Denmark) diluted 1/10 000 in blocking buffer.

Article Title: High Expression of Human AugminComplex Submit 3 Indicates Poor Prognosis and Associates with Tumor Progression in Hepatocellular Carcinoma
Article Snippet: .. The membrane was blocked with TBST (0.05% Tween 20 in TBS) containing 5% skim milk (BD) and then incubated overnight with the Haus3 antibody (ab170808, abcam, USA)at 4°C. .. The membrane was washed three times in TBST for 10 min and then incubated with an HRP-conjugated secondary antibody (Pierce) (1:5000) for 1 h at room temperature.

Article Title: Circadian Regulation of Myocardial Sarcomeric Titin-cap (Tcap, Telethonin): Identification of Cardiac Clock-Controlled Genes Using Open Access Bioinformatics Data
Article Snippet: .. The membranes were blocked for 1 h at room temperature with 5% dried non-fat milk in 5% TBST (10 mM Tris [pH 8.0], 150 mM NaCl, and 0.05% Tween-20), then washed 2x in 5% TBST, and incubated overnight at 4°C with monoclonal mouse anti-TCAP antibody (1∶2000; BD, Cat #612328). .. Immunoreactive bands were visualized with horseradish peroxidase-conjugated goat anti-mouse IgG (1∶5000; Sigma, A2304) and Clarity reagent (BioRad) on a Chemidoc MP system (BioRad).

Binding Assay:

Article Title: Efficacy and Safety of A Liposome-Based Vaccine against Protein Tau, Assessed in Tau.P301L Mice That Model Tauopathy
Article Snippet: .. Non-specific binding to membranes was blocked by incubation in 5% non-fat dry milk in TBST (0.1% Tween-20, 50 mM Tris.HCl, pH 7.6, 150 mM NaCl) prior to overnight incubation at 4°C with primary antibody as indicated: Tau5 (anti-pan Tau; BD bioscience, USA), HT7 (anti-human Tau; Thermo Scientific, USA), pS396 (Invitrogen, UK), AT180 (anti-pT231, Innogenetics, Belgium), pS404 (Sigma, USA) and GFAP (DAKO, Denmark) all diluted in the same blocking buffer. .. After washing in TBST, blots were incubated for 1 hour at RT with secondary goat anti-mouse or anti-rabbit, HRP-conjugated (DAKO, Denmark) diluted 1/10 000 in blocking buffer.

Stripping Membranes:

Article Title: T cell Immunoglobulin Mucin Protein (TIM)-4 binds phosphatidylserine and mediates uptake of apoptotic cells
Article Snippet: .. The PIP strip was then washed three times in TBST (140 mM NaCl, 10 mM Tris-HCl, 0.1% Tween20) with 0.5 mM CaCl2 before incubating with HRP-conjugated rat anti-mouse IgG2a mAb (BD Pharmingen). .. Antibody binding was detected using ECL plus Western blotting detection reagents (Amersham Bioscience) and visualized using Kodak X-Omat 2000A processor.

Blocking Assay:

Article Title: Efficacy and Safety of A Liposome-Based Vaccine against Protein Tau, Assessed in Tau.P301L Mice That Model Tauopathy
Article Snippet: .. Non-specific binding to membranes was blocked by incubation in 5% non-fat dry milk in TBST (0.1% Tween-20, 50 mM Tris.HCl, pH 7.6, 150 mM NaCl) prior to overnight incubation at 4°C with primary antibody as indicated: Tau5 (anti-pan Tau; BD bioscience, USA), HT7 (anti-human Tau; Thermo Scientific, USA), pS396 (Invitrogen, UK), AT180 (anti-pT231, Innogenetics, Belgium), pS404 (Sigma, USA) and GFAP (DAKO, Denmark) all diluted in the same blocking buffer. .. After washing in TBST, blots were incubated for 1 hour at RT with secondary goat anti-mouse or anti-rabbit, HRP-conjugated (DAKO, Denmark) diluted 1/10 000 in blocking buffer.

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    Becton Dickinson tbst
    Tbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Becton Dickinson
    Average 94 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    94
    Becton Dickinson tween 20 tbst
    Tween 20 Tbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 tbst/product/Becton Dickinson
    Average 94 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    tween 20 tbst - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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