Structured Review

Applygen Technologies tbst
Tbst, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Applygen Technologies
Average 93 stars, based on 69 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
93/100 stars

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Incubation:

Article Title: Celecoxib regulates apoptosis and autophagy via the PI3K/Akt signaling pathway in SGC-7901 gastric cancer cells
Article Snippet: .. After blocking overnight at 4°C using 5% BSA, the membranes were incubated with primary antibodies (anti-procaspase-8 1:2,500 and procaspase-9 1:2,000; both from Abcam, Cambridge, MA, USA), p-Akt 1:800 (Bioworld, St. Louis Park, MN, USA) for 2 h at room temperature, washed by TBST and incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2,000 dilution for 2 h. Bands were visualized using enhanced chemiluminescence (ECL; Applygen, Beijing, China) detection reagents and scanned images were quantified using ImageJ software. ..

Article Title: Combined treatment with artesunate and bromocriptine has synergistic anticancer effects in pituitary adenoma cell lines
Article Snippet: .. Following overnight incubation of the membranes with the primary antibodies (anti-GAPDH, 1:1000; anti-PTEN 1:1000) at 4°C, three washes with TBST were performed and the membranes were incubated with an HRP-conjugated secondary antibody at room temperature for 2 h. Following three washes with TBST, the antibody-bound proteins were detected with a Super ECL Plus Detection Reagent (Applygen Technologies Inc., China). .. The protein band density was determined with ImageJ 1.38X software.

Article Title: Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats
Article Snippet: .. After three washes in TBST, the membrane was incubated with corresponding secondary antibodies conjugated to horseradish peroxidase at room temperature for 40 min and chemiluminescence detection was performed using Super ECL Plus (Applygen Technologies). ..

Blocking Assay:

Article Title: Celecoxib regulates apoptosis and autophagy via the PI3K/Akt signaling pathway in SGC-7901 gastric cancer cells
Article Snippet: .. After blocking overnight at 4°C using 5% BSA, the membranes were incubated with primary antibodies (anti-procaspase-8 1:2,500 and procaspase-9 1:2,000; both from Abcam, Cambridge, MA, USA), p-Akt 1:800 (Bioworld, St. Louis Park, MN, USA) for 2 h at room temperature, washed by TBST and incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2,000 dilution for 2 h. Bands were visualized using enhanced chemiluminescence (ECL; Applygen, Beijing, China) detection reagents and scanned images were quantified using ImageJ software. ..

Software:

Article Title: Celecoxib regulates apoptosis and autophagy via the PI3K/Akt signaling pathway in SGC-7901 gastric cancer cells
Article Snippet: .. After blocking overnight at 4°C using 5% BSA, the membranes were incubated with primary antibodies (anti-procaspase-8 1:2,500 and procaspase-9 1:2,000; both from Abcam, Cambridge, MA, USA), p-Akt 1:800 (Bioworld, St. Louis Park, MN, USA) for 2 h at room temperature, washed by TBST and incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibody at 1:2,000 dilution for 2 h. Bands were visualized using enhanced chemiluminescence (ECL; Applygen, Beijing, China) detection reagents and scanned images were quantified using ImageJ software. ..

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  • 93
    Applygen Technologies tbst
    Tbst, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Applygen Technologies
    Average 93 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    88
    Applygen Technologies tbst solution thrice
    Tbst Solution Thrice, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst solution thrice/product/Applygen Technologies
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tbst solution thrice - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

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