Structured Review

Agilent technologies tbst
Tbst, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbst/product/Agilent technologies
Average 99 stars, based on 727 article reviews
Price from $9.99 to $1999.99
tbst - by Bioz Stars, 2020-05
99/100 stars

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Staining:

Article Title: Microarray Analyses Demonstrate the Involvement of Type I Interferons in Psoriasiform Pathology Development in D6-deficient Mice *
Article Snippet: .. Excess antibody was removed by washing twice in TBST, and staining was detected using the Dako Envision kit according to the manufacturer's instructions. .. The slides were dehydrated to xylene, mounted in DPX, and visualized on a light microscope (Carl Zeiss).

Article Title: Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity
Article Snippet: .. The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution, S2023); 2) wash in TBST; 3) blocking using serum for 20 min; 4) primary anti-F4/80 antibody incubation for 60 min; 5) wash in TBST for 5 min; 6) 30 min incubation with MOM ImmPress Polymer Reagent (MP-2400); 7) wash in TBST; 8) DAB solution (5–10 min) prepared according to the manufacturer’s instruction (DAB Peroxidase substrate kit, SK-4100); 9) wash in TBST; 10) 1 min incubation with Dako REAL Haematoxylin (S2020). ..

Incubation:

Article Title: The Anti-Tumor Effects and Molecular Mechanisms of Suberoylanilide Hydroxamic Acid (SAHA) on the Aggressive Phenotypes of Ovarian Carcinoma Cells
Article Snippet: .. For immunoblotting, the membranes were incubated for 1 h with the primary antibody (Table S2 in ), rinsed with TBST, and incubated with anti-rabbit, anti-mouse or anti-goat IgG antibodies conjugated to horseradish peroxidase (HRP; Dako, Carpinteria CA, USA) at a dilution of 1:1000. .. After applying electrochemiluminescent (ECL)-Plus detection reagents (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the protein bands were visualized using X-ray film (Fujifilm, Tokyo, Japan).

Article Title: Translational Signalling, Atrogenic and Myogenic Gene Expression during Unloading and Reloading of Skeletal Muscle in Myostatin-Deficient Mice
Article Snippet: .. After incubating with the primary antibody, membranes were then washed in TBST, incubated with HRP-conjugated goat anti-rabbit secondary antibody (# PO448, DakoCytomation, Med-Bio, Christchurch, New Zealand) at 1∶5,000 for 2 h, then washed again and developed with enhanced chemiluminescence. .. The optical densities of each immunoreactive band were captured with a densitometer (GS 800, Bio-Rad Laboratories (NZ) Pty Ltd, Auckland, NZ) and analysed using Quantity One software (Bio-Rad Laboratories (NZ) Pty Ltd).

Article Title: Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity
Article Snippet: .. The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution, S2023); 2) wash in TBST; 3) blocking using serum for 20 min; 4) primary anti-F4/80 antibody incubation for 60 min; 5) wash in TBST for 5 min; 6) 30 min incubation with MOM ImmPress Polymer Reagent (MP-2400); 7) wash in TBST; 8) DAB solution (5–10 min) prepared according to the manufacturer’s instruction (DAB Peroxidase substrate kit, SK-4100); 9) wash in TBST; 10) 1 min incubation with Dako REAL Haematoxylin (S2020). ..

Article Title: Cytotoxic G-rich oligodeoxynucleotides: putative protein targets and required sequence motif
Article Snippet: .. Membranes were washed 3 × 5 min in TBST and incubated for 1 h at room temperature with horseradish peroxidase conjugated antibodies (1:2000 in 5% skim milk-TBST; DakoCytomation). .. Membranes were washed 3 × 5 min in TBST and visualized by chemiluminescence using ECL (Amersham) and Hypersensitive film (Amersham).

Article Title: Comparative Expression Profiling of Distinct T Cell Subsets Undergoing Oxidative Stress
Article Snippet: .. Following at least 3 washing steps in TBST the membranes were incubated with suited horseradish peroxidase (HRP)-conjugated secondary antibodies (P0217, P0260, P0449, DAKO, Hamburg, Germany; ab6877, Abcam), washed again at least 3 times in TBST prior to the visualization of the respective immunostainings using a chemiluminescent detection kit (LumiLight Western blot substrate, Roche, Germany) according to the manufacturer's recommendations. .. The quantification of the respective protein expression levels was determined by applying the advanced image data analyzer (AIDA) software package (Image analyser V. 4.11, raytest Isotopenmessgeraete GmbH, Berlin, Germany).

Article Title: High frequency of tumor cells with nuclear Egr-1 protein expression in human bladder cancer is associated with disease progression
Article Snippet: .. The TMA was washed twice in TBST and blocked in protein block for 5 minutes, followed by 15 minutes incubation with anti-Egr-1 antibody in antibody diluent (Dako A/S, Denmark, catalogue number S3022). .. The TMA were washed 3 times in TBST and underwent 5 sequential incubations with 3× washes in TBST between each step: 15 minutes with biotinylated goat anti-rabbit IgG (diluted in TBST); 15 minutes with streptavidin-biotin complex; 15 minutes with amplification reagent; 15 minutes with streptavidin-peroxidase; and 5 minutes with substrate chromagen solution.

Blocking Assay:

Article Title: Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity
Article Snippet: .. The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution, S2023); 2) wash in TBST; 3) blocking using serum for 20 min; 4) primary anti-F4/80 antibody incubation for 60 min; 5) wash in TBST for 5 min; 6) 30 min incubation with MOM ImmPress Polymer Reagent (MP-2400); 7) wash in TBST; 8) DAB solution (5–10 min) prepared according to the manufacturer’s instruction (DAB Peroxidase substrate kit, SK-4100); 9) wash in TBST; 10) 1 min incubation with Dako REAL Haematoxylin (S2020). ..

Article Title: High frequency of tumor cells with nuclear Egr-1 protein expression in human bladder cancer is associated with disease progression
Article Snippet: .. The TMA was washed twice in TBST and blocked in protein block for 5 minutes, followed by 15 minutes incubation with anti-Egr-1 antibody in antibody diluent (Dako A/S, Denmark, catalogue number S3022). .. The TMA were washed 3 times in TBST and underwent 5 sequential incubations with 3× washes in TBST between each step: 15 minutes with biotinylated goat anti-rabbit IgG (diluted in TBST); 15 minutes with streptavidin-biotin complex; 15 minutes with amplification reagent; 15 minutes with streptavidin-peroxidase; and 5 minutes with substrate chromagen solution.

Western Blot:

Article Title: Comparative Expression Profiling of Distinct T Cell Subsets Undergoing Oxidative Stress
Article Snippet: .. Following at least 3 washing steps in TBST the membranes were incubated with suited horseradish peroxidase (HRP)-conjugated secondary antibodies (P0217, P0260, P0449, DAKO, Hamburg, Germany; ab6877, Abcam), washed again at least 3 times in TBST prior to the visualization of the respective immunostainings using a chemiluminescent detection kit (LumiLight Western blot substrate, Roche, Germany) according to the manufacturer's recommendations. .. The quantification of the respective protein expression levels was determined by applying the advanced image data analyzer (AIDA) software package (Image analyser V. 4.11, raytest Isotopenmessgeraete GmbH, Berlin, Germany).

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    Agilent technologies tbst
    Tbst, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Agilent technologies
    Average 99 stars, based on 742 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

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