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<t>DNA</t> <t>annealing</t> evaluation by 4-20% <t>TBE</t> gel Lane 1: LSP (−43 to +20) single strand (ss) template (T) DNA, Lane 2: LSP (−43 to +20) ss non-template (NT) DNA, Lane 3: LSP (−43 to +20) duplex DNA.
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<t>DNA</t> <t>annealing</t> evaluation by 4-20% <t>TBE</t> gel Lane 1: LSP (−43 to +20) single strand (ss) template (T) DNA, Lane 2: LSP (−43 to +20) ss non-template (NT) DNA, Lane 3: LSP (−43 to +20) duplex DNA.
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<t>DNA</t> <t>annealing</t> evaluation by 4-20% <t>TBE</t> gel Lane 1: LSP (−43 to +20) single strand (ss) template (T) DNA, Lane 2: LSP (−43 to +20) ss non-template (NT) DNA, Lane 3: LSP (−43 to +20) duplex DNA.
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DNA annealing evaluation by 4-20% TBE gel Lane 1: LSP (−43 to +20) single strand (ss) template (T) DNA, Lane 2: LSP (−43 to +20) ss non-template (NT) DNA, Lane 3: LSP (−43 to +20) duplex DNA.

Journal: STAR Protocols

Article Title: Protocol to investigate human mitochondrial transcription initiation by integrating biochemical and cryo-EM approaches

doi: 10.1016/j.xpro.2025.104347

Figure Lengend Snippet: DNA annealing evaluation by 4-20% TBE gel Lane 1: LSP (−43 to +20) single strand (ss) template (T) DNA, Lane 2: LSP (−43 to +20) ss non-template (NT) DNA, Lane 3: LSP (−43 to +20) duplex DNA.

Article Snippet: Maintain the duplex DNA concentration above 10 μM. c. Verify the annealing of the DNA strands by running a sample on a 4-20% TBE gel (Invitrogen) at 100 V for 4 h at 4°C, using TBE as the running buffer ( ).

Techniques: