tbars (tca method) assay kit cayman 700870  (Cayman Chemical)

Journal: Communications Biology

Article Title: Extended supercooled storage of red blood cells

doi: 10.1038/s42003-024-06463-4

Figure Lengend Snippet: a The samples were stored for 6 weeks, and hemolysis was measured. b The samples were stored for 10 weeks, and hemolysis was measured. c Data shows lactate level corrected for hemolysis after 6 weeks. Measured total lactate level was normalized by fraction of unlysed RBCs. d Data shows TBARS levels after 6 weeks. e Data shows MCHC levels at both temperatures after 6 weeks. f Data shows MCH levels at both temperatures after 6 weeks. Colored and dashed lines represent the mean day 1 levels following 3x washing, overnight storage at +4 °C, and final 1x washing in the respective solutions. Data represent mean ± standard deviation from 3 biological replicates ( N = 3) and three technical replicates ( n = 3, n = 1-3 for lactate, n = 2 for TBARS) for each biological replicate. Each biological replicate was from a pool of 3 donor samples. A two-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was performed to evaluate significant differences between conditions: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Comparisons were shown across the two temperatures for each solution and for E-Sol 5 against the other solutions. TBARS Thiobarbituric Acid Reactive Substances. MCHC mean cell hemoglobin concentration. MCH mean corpuscular hemoglobin. See Supplementary Fig. for comparisons across the solutions at each temperature.

Article Snippet: Lipid peroxidation was quantified by a Thiobarbituric Acid Reactive Substances (TBARS) assay kit (700870, Cayman Chemical, MI, USA).

Techniques: Standard Deviation, Concentration Assay

Journal: Communications Biology

Article Title: Extended supercooled storage of red blood cells

doi: 10.1038/s42003-024-06463-4

Figure Lengend Snippet: a Hemolysis increased with increasing time of storage and decreasing temperature. b Data shows lactate levels normalized by fraction of unlysed red blood cells after 3, 7, and 10 weeks. Measured total lactate level was normalized by percent of unlysed RBCs. c Data shows TBARS levels after 3, 7, and 10 weeks. d Data shows TAS levels after 3, 7, and 10 weeks. Dashed lines represent the mean day 1 levels following 3x washing, overnight storage at +4 °C, and final 1x washing in E-Sol 5. Data represent mean ± standard deviation from 3 biological replicates ( N = 3) and 1-3 technical replicates for each biological replicate ( n = 3 for hemolysis, n = 1-3 for lactate, n = 2 for TBARS and TAS). Each biological replicate was from a pool of 3 donor samples. A two-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was performed to evaluate significant differences between conditions: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Comparisons were shown across the three temperatures at each time point. See Supplementary Fig. for comparisons across the time points at each temperature. TBARS Thiobarbituric Acid Reactive Substances. TAC Total Antioxidant Capacity.

Article Snippet: Lipid peroxidation was quantified by a Thiobarbituric Acid Reactive Substances (TBARS) assay kit (700870, Cayman Chemical, MI, USA).

Techniques: Standard Deviation