taq polymerase  (Millipore)


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    Structured Review

    Millipore taq polymerase
    Comparison of SD polymerase and <t>Taq</t> DNA polymerase with respect to amplification efficiency ( a ) and influence of annealing temperature ( b ). a Superscript 1: <t>T-ARMS</t> PCR with SD polymerase. Superscript 2: T-ARMS PCR with Taq DNA polymerase. M molecular ladder of 100 bp, NTC no template control. b (i) SD polymerase at various TA on heterozygous (H) genotype (ii) Taq DNA Polymerase at various TA on heterozygous (H) and Wild (W) genotypes (Ge) in presence of DMSO (5%). M 100 bp ladder, NTC non-template control
    Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq polymerase/product/Millipore
    Average 99 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase"

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase

    Journal: BMC Research Notes

    doi: 10.1186/s13104-018-3236-6

    Comparison of SD polymerase and Taq DNA polymerase with respect to amplification efficiency ( a ) and influence of annealing temperature ( b ). a Superscript 1: T-ARMS PCR with SD polymerase. Superscript 2: T-ARMS PCR with Taq DNA polymerase. M molecular ladder of 100 bp, NTC no template control. b (i) SD polymerase at various TA on heterozygous (H) genotype (ii) Taq DNA Polymerase at various TA on heterozygous (H) and Wild (W) genotypes (Ge) in presence of DMSO (5%). M 100 bp ladder, NTC non-template control
    Figure Legend Snippet: Comparison of SD polymerase and Taq DNA polymerase with respect to amplification efficiency ( a ) and influence of annealing temperature ( b ). a Superscript 1: T-ARMS PCR with SD polymerase. Superscript 2: T-ARMS PCR with Taq DNA polymerase. M molecular ladder of 100 bp, NTC no template control. b (i) SD polymerase at various TA on heterozygous (H) genotype (ii) Taq DNA Polymerase at various TA on heterozygous (H) and Wild (W) genotypes (Ge) in presence of DMSO (5%). M 100 bp ladder, NTC non-template control

    Techniques Used: Amplification, Polymerase Chain Reaction

    2) Product Images from "T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase"

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase

    Journal: BMC Research Notes

    doi: 10.1186/s13104-018-3236-6

    Comparison of SD polymerase and Taq DNA polymerase with respect to amplification efficiency ( a ) and influence of annealing temperature ( b ). a Superscript 1: T-ARMS PCR with SD polymerase. Superscript 2: T-ARMS PCR with Taq DNA polymerase. M molecular ladder of 100 bp, NTC no template control. b (i) SD polymerase at various TA on heterozygous (H) genotype (ii) Taq DNA Polymerase at various TA on heterozygous (H) and Wild (W) genotypes (Ge) in presence of DMSO (5%). M 100 bp ladder, NTC non-template control
    Figure Legend Snippet: Comparison of SD polymerase and Taq DNA polymerase with respect to amplification efficiency ( a ) and influence of annealing temperature ( b ). a Superscript 1: T-ARMS PCR with SD polymerase. Superscript 2: T-ARMS PCR with Taq DNA polymerase. M molecular ladder of 100 bp, NTC no template control. b (i) SD polymerase at various TA on heterozygous (H) genotype (ii) Taq DNA Polymerase at various TA on heterozygous (H) and Wild (W) genotypes (Ge) in presence of DMSO (5%). M 100 bp ladder, NTC non-template control

    Techniques Used: Amplification, Polymerase Chain Reaction

    T-ARMS PCR strategy for SNP rs445709131 . a Conceptual diagram of T-ARMS using Taq polymerase (i) and SD polymerase (ii). b Genotyping pattern by Taq polymerase (i) and SD polymerase (ii). The outer primers (OF and OR) amplified a 354 bp product. The IF primer generated wild allele with amplicon size of 179 bp while the IR primer generated mutated allele with an amplicon size of 230 bp. N wild genotype, C carrier genotype, M molecular ladder of 100 bp
    Figure Legend Snippet: T-ARMS PCR strategy for SNP rs445709131 . a Conceptual diagram of T-ARMS using Taq polymerase (i) and SD polymerase (ii). b Genotyping pattern by Taq polymerase (i) and SD polymerase (ii). The outer primers (OF and OR) amplified a 354 bp product. The IF primer generated wild allele with amplicon size of 179 bp while the IR primer generated mutated allele with an amplicon size of 230 bp. N wild genotype, C carrier genotype, M molecular ladder of 100 bp

    Techniques Used: Polymerase Chain Reaction, Amplification, Generated

    Related Articles

    Amplification:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Chromatography:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Purification:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Concentration Assay:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Generated:

    Article Title: Evasion of myofibroblasts from immune surveillance: A mechanism for tissue fibrosis
    Article Snippet: .. Rat KM81 (IgG2b), anti-mouse pan CD44 mAbs (constant region-specific), and rat 2C11a (IgG1) anti-mouse CD3 mAb were generated from American Type Culture Collection hybridomas and further purified by protein S -Sepharose chromatography , Tri reagent (T9424; Sigma–Aldrich), a reverse transcription system (Promega, Madison, WI), and Taq DNA polymerase and ethidium bromide (Sigma–Aldrich). .. The 11- to 12-week-old male C57BL/6 and BALB/c mice were purchased from Harlan–Sprague–Dawley.

    Polymerase Chain Reaction:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Molecular characterization of leaf spot fungi using internal transcribed spacer (ITS) based phylogenetic inference
    Article Snippet: .. The PCR amplification was performed in a Bio-RAD instrument with a total 25 µl reaction comprised of 20 ng of genomic DNA template, 10X buffer with 25mM MgCl2, 10mM dNTP's, 2U of Taq DNA polymerase and 10 pmol of each primer (Sigma -Aldrich). .. The following PCR reaction conditions were used: 4 min at 94°C for denaturation, 30 cycles each of 30 sec at 94°C for denaturation, 1min at 58.2°C for annealing, 2 min at 72°C for extension followed by the final extension at 72°C for 7 min.

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
    Article Snippet: .. We have reported the standard procedure of T-ARMS PCR for genotyping of the SNP rs445709131 using Taq polymerase (Sigma-Aldrich, USA, Cat.No.D6677) [ ]. .. The SD polymerase (Bioron GmbH, Germany, Cat. No. 108702) T-ARMS PCR reaction mix was different from the standard T-ARMS (Table ).

    Plasmid Preparation:

    Article Title: Vaccine Efficacy against Malaria by the Combination of Porcine Parvovirus-Like Particles and Vaccinia Virus Vectors Expressing CS of Plasmodium
    Article Snippet: .. For quantification of parasite-specific rRNA by quantitative-competitive reverse transcription-PCR (RT-PCR) , parasite-specific rRNA was amplified from 5 µl of the cDNA mixture in a PCR master mix containing 46 µl of PCR Supermix (Gibco/BRL), 1 µl each of parasite-specific primers PB1 and PB2 (1) (12 µM, final concentration), 0.2 µl (1 U) of Taq DNA poly merase (Sigma, St. Louis, Mo.), and 1 µl of a known concentration of competitor plasmid. .. Then 35 cycles of amplification in a PCR Express (Hybaid, Middlesex, United Kingdom) thermocycler were performed under the following conditions: 94°C for 1 min, 60°C for 2 min, and 72°C for 1 min. An initial denaturation step at 94°C for 2 min and a terminal elongation step at 72°C for 10 min were also included.

    Article Title: Diagnostic potential of Brucella melitensis Rev1 native Omp28 precursor in human brucellosis
    Article Snippet: .. GenElute™ Plasmid Miniprep Kit, Lysozyme from chicken egg white, dNTP Mix, Taq DNA Polymerase, ampicillin, kanamycin sulphate, and albumin from bovine serum (BSA) were purchased from Sigma-Aldrich, USA. .. Antigen of RBPT was obtained from Pendik Veterinary Control and Research Institute.

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  • 96
    Millipore taq dna polymerase
    Taq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ex Taq Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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