taq polymerase hotstar taq dna polymerase qiagen valencia ca  (Qiagen)


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    Qiagen taq polymerase hotstar taq dna polymerase qiagen valencia ca
    Taq Polymerase Hotstar Taq Dna Polymerase Qiagen Valencia Ca, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq polymerase hotstar taq dna polymerase qiagen valencia ca - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice
    Article Snippet: Quantitative PCR Sets of qPCR primers (Table ) were used to quantitate bacterial populations, based on the universal bacterial 16S rRNA sequences [ ], C. jejuni luxS [ ], and A. baumannii oxa51 [ ]. qPCRs were performed using 3.5 mM MgCl2 , 0.4 ng/µl bovine serum albumin, 0.2 mM of each deoxynucleoside triphosphate, 10 pmol of each primer, 0.625 U Taq DNA polymerase (Qiagen, Valencia CA, USA), and 2 µl extracted DNA in a final 20-µl volume of SYBR green master mix. qPCR conditions included 5 min at 94 °C and 45 cycles of 10 s at 94 °C, 10 s at 60 °C (C. jejuni and A. baumannii ) or 56 °C (total bacteria). .. Quantitative PCR Sets of qPCR primers (Table ) were used to quantitate bacterial populations, based on the universal bacterial 16S rRNA sequences [ ], C. jejuni luxS [ ], and A. baumannii oxa51 [ ]. qPCRs were performed using 3.5 mM MgCl2 , 0.4 ng/µl bovine serum albumin, 0.2 mM of each deoxynucleoside triphosphate, 10 pmol of each primer, 0.625 U Taq DNA polymerase (Qiagen, Valencia CA, USA), and 2 µl extracted DNA in a final 20-µl volume of SYBR green master mix. qPCR conditions included 5 min at 94 °C and 45 cycles of 10 s at 94 °C, 10 s at 60 °C (C. jejuni and A. baumannii ) or 56 °C (total bacteria).

    Clone Assay:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: Plasmids were purified using a QiaPrep Spin plasmid kit, and DNA fragments were prepared for cloning by agarose gel extraction using a Qiaquick gel extraction kit (Qiagen). .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Article Title: Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence
    Article Snippet: PCR was performed using Taq polymerase (QIAGEN) or Pfu or Platinum Pfx polymerase (Stratagene) according to the manufacturers' instructions. .. All clones derived from PCR fragments were verified by sequencing.

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: The resulting PCR products were ligated to pGEM-T Easy Vector (Promega, Japan) at 4°C for overnight and then transformed in E. coli DH5α competent cells (Toyobo, Japan) and cloned. .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.).

    Amplification:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: .. Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Rob W. Briddon, Faisalabad, Pakistan, containing the sequence of ACMV-[Nigeria-Ogo]; AJ427910) as template, forward 5′-A CCCGGGTCGAC ATGTCGAAGCGACCAGGA-3′ and reverse primer 5′-A CCCGGG TTAATTGCCAATACTGTCATA-3′ adding Sma I and Sal I (underlined) restriction sites.

    Article Title: KEAP1 gene mutations and NRF2 activation are common in pulmonary papillary adenocarcinoma
    Article Snippet: .. Primers for PCR amplification and sequencing were designed in the area of exon 3 of KEAP1 [ ] and synthesized by Invitrogen (Carlsbad, California, United States), and amplification of DNA from early passage A549 cells (American Type Culture Collection, Manassas, Virginia, USA) and from primary tumor samples was performed using Taq polymerase (Qiagen, California, United States) as previously described [ ]. .. All potential genetic alterations were confirmed by bi-directional sequencing and testing a second, independent DNA sample from the tumor.

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: Paragraph title: Random amplified polymorphic DNA (RAPD) fingerprinting, PCR of bacterial marker genes, and sequencing of the transferred rpsL gene. ... RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ).

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: The internal transcribed spacer (ITS) regions were then amplified using the ITS1F and ITS4 primers (White et al. ; Gardes & Bruns ). .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada).

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA). .. The amplified products were analyzed by electrophoresis in 10% polyacrylamide gel (visualized by gel-red).

    Article Title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon
    Article Snippet: The ITS regions were amplified by means of genomic DNA as a template and universal primers ITS1 and ITS4, while the D1/D2 domains were augmented through the primers NL-1 and NL-4 on the genomic DNA. .. Twenty microlitre of PCR contained 1 µL DNA template (50 ng), 200 mM of each deoxynucleotide triphosphate, 2 µL of tenfold buffer (Taq DNA Polymerase, Qiagen, Chatsworth, CA, USA), 0.7 mM each primer, and 1.0 U Taq DNA Polymerase (Qiagen).

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.). .. Analysis of sequencing results Sequences obtained for ccgf analysis were analyzed by MUSCLE alignment and phylogenetic trees were drawn by neighbor-joining method using MEGA5.1 software .

    Synthesized:

    Article Title: KEAP1 gene mutations and NRF2 activation are common in pulmonary papillary adenocarcinoma
    Article Snippet: .. Primers for PCR amplification and sequencing were designed in the area of exon 3 of KEAP1 [ ] and synthesized by Invitrogen (Carlsbad, California, United States), and amplification of DNA from early passage A549 cells (American Type Culture Collection, Manassas, Virginia, USA) and from primary tumor samples was performed using Taq polymerase (Qiagen, California, United States) as previously described [ ]. .. All potential genetic alterations were confirmed by bi-directional sequencing and testing a second, independent DNA sample from the tumor.

    Construct:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. The correctness of the construct was confirmed using Li-Cor DNA-Sequencer Modell 4000L (Li-Cor Bioscience GmbH, Bad Homburg, Germany).

    SYBR Green Assay:

    Article Title: Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice
    Article Snippet: .. Quantitative PCR Sets of qPCR primers (Table ) were used to quantitate bacterial populations, based on the universal bacterial 16S rRNA sequences [ ], C. jejuni luxS [ ], and A. baumannii oxa51 [ ]. qPCRs were performed using 3.5 mM MgCl2 , 0.4 ng/µl bovine serum albumin, 0.2 mM of each deoxynucleoside triphosphate, 10 pmol of each primer, 0.625 U Taq DNA polymerase (Qiagen, Valencia CA, USA), and 2 µl extracted DNA in a final 20-µl volume of SYBR green master mix. qPCR conditions included 5 min at 94 °C and 45 cycles of 10 s at 94 °C, 10 s at 60 °C (C. jejuni and A. baumannii ) or 56 °C (total bacteria). .. All assays were performed using a Light Cycler 480 (Roche Diagnostic Corporation, Indianapolis IN, USA).

    Incubation:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Microglia were incubated in the presence or absence of recombinant IFN-γ, for 24 h, or infected with strain BeAn for 48 h for time periods predetermined to be optimal for gene expression under each condition. .. Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Article Title: Multiresistant Bacteria Isolated from Chicken Meat in Austria
    Article Snippet: Conditions for PCR: initial denaturation at 94 °C for 5 min; 35 cycles at 95 °C for 30 s, 52 °C for 45 s, and 72 °C for 60 s; and final incubation for 10 min at 72 °C. .. Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany) were used.

    Expressing:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: .. Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Rob W. Briddon, Faisalabad, Pakistan, containing the sequence of ACMV-[Nigeria-Ogo]; AJ427910) as template, forward 5′-A CCCGGGTCGAC ATGTCGAAGCGACCAGGA-3′ and reverse primer 5′-A CCCGGG TTAATTGCCAATACTGTCATA-3′ adding Sma I and Sal I (underlined) restriction sites.

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Microglia were incubated in the presence or absence of recombinant IFN-γ, for 24 h, or infected with strain BeAn for 48 h for time periods predetermined to be optimal for gene expression under each condition. .. Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Transformation Assay:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Fragments were inserted into pGEM-T (Promega Corporation, Mannheim, Germany), transformed into E. coli , excised with Sma I and ligated into Sma I-digested pESP1-pREP2 [ ] yielding pESP1-pREP2:AV1.

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: The resulting PCR products were ligated to pGEM-T Easy Vector (Promega, Japan) at 4°C for overnight and then transformed in E. coli DH5α competent cells (Toyobo, Japan) and cloned. .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.).

    Activated Clotting Time Assay:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. The sequences of the cytokine primers and the expected product sizes are as follows: IFN-α, 5′ primer 5′ GAC TCA TCT GCT GCT TGG AAT GCA ACC CTC C 3′ and 3′ primer 5′ GAC TCA CTC CTT CTC CTC ACT CAG TCT TGC C 3′ (294 bp); IFN-β, 5′ primer 5′ CAG CTC CAG CTC CAA GAA AGG ACG AAC ATT CG 3′ and 3′ primer 5′ CCA CCA CTC ATT CTG AGG CAT CAA CTG ACA GG 3′ (509 bp); IL-1β, 5′ primer 5′ AAG CTC TCC ACC TCA ATG GAC AG 3′ and 3′ primer 5′ CTC AAA CTC CAC TTT GCT CTT GA 3′ (260 bp); IL-6, 5′ primer 5′ CCT CTG GTC TTC TGG AGT ACC AT 3′ and 3′ primer 5′ GGC ATA ACG CAC TAG GTT TGC CG 3′ (307 bp); IL-10, 5′ primer 5′ CCA GTT TTA CCT GGT AGA AGT GAT G 3′ and 3′ primer 5′ TGT CTA GGT CCT GGA GTC CAG CAG ACT CAA 3′ (324 bp); IL-12 p40, 5′ primer 5′ ATG GCC ATG TGG GAG CTG GAG AAA G 3′ and 3′ 5′ GTG GAG CAG CAG ATG TGA GTG GCT 3′ (451 bp); IL-18, 5′ primer 5′ CTG TGT TCG AGG ATA TGA CTG 3′ and 3′ primer 5′ GTG TCC TTC ATA CAG TGA AG 3′ (283 bp); TNF-α, 5′ primer 5′ GTT CTA TGG CCC AGA CCC TCA CA 3′ and 3′ primer 5′ TAC CAG GGT TTG AGC TCA GC 3′ (364 bp); inducible no synthase (iNOS), 5′ primer 5′ TGG GAA TGG AGA CTG TCC CAG 3′ and 3′ primer 5′ GGG ATC TGA ATG TGA TGT TTG 3′ (306 bp); MIP-1α, 5′ primer 5′ ATG AAG GTC TCC ACC ACT GCC CTT G 3′ and 3′ primer 5′ GGC ATT CAG TCC AGG TCA GTG AT 3′ (276 bp); IFN-γ, 5′ primer 5′ CTT GGA TAT CTG GAG GAA CTG GC 3′ and 3′ primer 5′ GCG CTG GAC CTG TGG GTT GTT GA 3′ (271 bp); hypoxanthine phosphoribosyltransferase (HPRT), 5′ primer 5′ GTT GGA TAC AGG CCA GAC TTT GTT G 3′ and 3′ primer 5′ GAG GGT AGG CTG GCC TAT AGG CT 3′ (352 bp).

    Derivative Assay:

    Article Title: Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence
    Article Snippet: PCR was performed using Taq polymerase (QIAGEN) or Pfu or Platinum Pfx polymerase (Stratagene) according to the manufacturers' instructions. .. All clones derived from PCR fragments were verified by sequencing.

    Countercurrent Chromatography:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. The sequences of the cytokine primers and the expected product sizes are as follows: IFN-α, 5′ primer 5′ GAC TCA TCT GCT GCT TGG AAT GCA ACC CTC C 3′ and 3′ primer 5′ GAC TCA CTC CTT CTC CTC ACT CAG TCT TGC C 3′ (294 bp); IFN-β, 5′ primer 5′ CAG CTC CAG CTC CAA GAA AGG ACG AAC ATT CG 3′ and 3′ primer 5′ CCA CCA CTC ATT CTG AGG CAT CAA CTG ACA GG 3′ (509 bp); IL-1β, 5′ primer 5′ AAG CTC TCC ACC TCA ATG GAC AG 3′ and 3′ primer 5′ CTC AAA CTC CAC TTT GCT CTT GA 3′ (260 bp); IL-6, 5′ primer 5′ CCT CTG GTC TTC TGG AGT ACC AT 3′ and 3′ primer 5′ GGC ATA ACG CAC TAG GTT TGC CG 3′ (307 bp); IL-10, 5′ primer 5′ CCA GTT TTA CCT GGT AGA AGT GAT G 3′ and 3′ primer 5′ TGT CTA GGT CCT GGA GTC CAG CAG ACT CAA 3′ (324 bp); IL-12 p40, 5′ primer 5′ ATG GCC ATG TGG GAG CTG GAG AAA G 3′ and 3′ 5′ GTG GAG CAG CAG ATG TGA GTG GCT 3′ (451 bp); IL-18, 5′ primer 5′ CTG TGT TCG AGG ATA TGA CTG 3′ and 3′ primer 5′ GTG TCC TTC ATA CAG TGA AG 3′ (283 bp); TNF-α, 5′ primer 5′ GTT CTA TGG CCC AGA CCC TCA CA 3′ and 3′ primer 5′ TAC CAG GGT TTG AGC TCA GC 3′ (364 bp); inducible no synthase (iNOS), 5′ primer 5′ TGG GAA TGG AGA CTG TCC CAG 3′ and 3′ primer 5′ GGG ATC TGA ATG TGA TGT TTG 3′ (306 bp); MIP-1α, 5′ primer 5′ ATG AAG GTC TCC ACC ACT GCC CTT G 3′ and 3′ primer 5′ GGC ATT CAG TCC AGG TCA GTG AT 3′ (276 bp); IFN-γ, 5′ primer 5′ CTT GGA TAT CTG GAG GAA CTG GC 3′ and 3′ primer 5′ GCG CTG GAC CTG TGG GTT GTT GA 3′ (271 bp); hypoxanthine phosphoribosyltransferase (HPRT), 5′ primer 5′ GTT GGA TAC AGG CCA GAC TTT GTT G 3′ and 3′ primer 5′ GAG GGT AGG CTG GCC TAT AGG CT 3′ (352 bp).

    Oligonucleotide Synthesis:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen. .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Infection:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Microglia were incubated in the presence or absence of recombinant IFN-γ, for 24 h, or infected with strain BeAn for 48 h for time periods predetermined to be optimal for gene expression under each condition. .. Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Generated:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: First-strand cDNA was generated from 1 μg of total RNA from the microglia by using oligo(dT)12–18 primers and an Advantage for RT for PCR Kit (Clontech Laboratories, Palo Alto, Calif.). .. Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Polymerase Chain Reaction:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: .. Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Rob W. Briddon, Faisalabad, Pakistan, containing the sequence of ACMV-[Nigeria-Ogo]; AJ427910) as template, forward 5′-A CCCGGGTCGAC ATGTCGAAGCGACCAGGA-3′ and reverse primer 5′-A CCCGGG TTAATTGCCAATACTGTCATA-3′ adding Sma I and Sal I (underlined) restriction sites.

    Article Title: Attenuation of teratoma formation by p27 overexpression in induced pluripotent stem cells
    Article Snippet: .. Polymerase chain reaction (PCR) was performed with Taq DNA polymerase (Qiagen). ..

    Article Title: Long‐term demographic decline and late glacial divergence in a Californian paleoendemic: Sequoiadendron giganteum (giant sequoia)
    Article Snippet: .. PCRs consisted of a 10 μ L solution containing 200 μ mol/L of each dNTP (Roche), 0.5 units of TAQ DNA polymerase (Qiagen, Redwood City, CA), 1 μ L −10x PCR buffer with 15 mmol/L MgCl2 , 0.4 μ mol/L reverse, 0.16 μ mol/L FAM‐labeled M13 primer, and approximately 20 ng of extracted DNA. .. Final extension consisted of 30 min at 72 C. Then, 1.5 μ L of the PCR product was mixed with a solution of 8 μ L formamide and 0.5 μ L Genescan LIZ500 size standard (Applied Biosystems, Waltham, MA).

    Article Title: KEAP1 gene mutations and NRF2 activation are common in pulmonary papillary adenocarcinoma
    Article Snippet: .. Primers for PCR amplification and sequencing were designed in the area of exon 3 of KEAP1 [ ] and synthesized by Invitrogen (Carlsbad, California, United States), and amplification of DNA from early passage A549 cells (American Type Culture Collection, Manassas, Virginia, USA) and from primary tumor samples was performed using Taq polymerase (Qiagen, California, United States) as previously described [ ]. .. All potential genetic alterations were confirmed by bi-directional sequencing and testing a second, independent DNA sample from the tumor.

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: Paragraph title: Random amplified polymorphic DNA (RAPD) fingerprinting, PCR of bacterial marker genes, and sequencing of the transferred rpsL gene. ... RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ).

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen. .. T4 DNA ligase was from Gibco BRL, and shrimp alkaline phosphatase was from Amersham.

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada). .. An equal amount of amplified DNA from each sample was combined into a single pool and sent for pyrosequencing using Roche 454 GS FLX+ chemistry at the Genome Québec Innovation Center (McGill University, Montréal, QC, Canada).

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: .. Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. PCR cycle conditions were 30 cycles of 94°C for 40 s, 60°C for 20 s, and 72°C for 40 s, followed by a final extension at 72°C for 5 min. PCR products were separated on an ethidium bromide-containing 2% agarose gel, illuminated on a UV light source, and photographed using Polaroid type 667 film.

    Article Title: Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence
    Article Snippet: .. PCR was performed using Taq polymerase (QIAGEN) or Pfu or Platinum Pfx polymerase (Stratagene) according to the manufacturers' instructions. .. All clones derived from PCR fragments were verified by sequencing.

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: Paragraph title: DNA extraction and PCR analysis ... The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA).

    Article Title: Multiresistant Bacteria Isolated from Chicken Meat in Austria
    Article Snippet: Conditions for PCR: initial denaturation at 94 °C for 5 min; 35 cycles at 95 °C for 30 s, 52 °C for 45 s, and 72 °C for 60 s; and final incubation for 10 min at 72 °C. .. Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany) were used.

    Article Title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon
    Article Snippet: .. Twenty microlitre of PCR contained 1 µL DNA template (50 ng), 200 mM of each deoxynucleotide triphosphate, 2 µL of tenfold buffer (Taq DNA Polymerase, Qiagen, Chatsworth, CA, USA), 0.7 mM each primer, and 1.0 U Taq DNA Polymerase (Qiagen). .. PCR program for ITS regions followed: 95 °C, 3 min; 34 cycles; 94 °C, 15 s; 55 °C, 45 s; 72 °C, 55 s; 72 °C, 7 min.

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.). .. Analysis of sequencing results Sequences obtained for ccgf analysis were analyzed by MUSCLE alignment and phylogenetic trees were drawn by neighbor-joining method using MEGA5.1 software .

    DNA Sequencing:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen. .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Sequencing:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Rob W. Briddon, Faisalabad, Pakistan, containing the sequence of ACMV-[Nigeria-Ogo]; AJ427910) as template, forward 5′-A CCCGGGTCGAC ATGTCGAAGCGACCAGGA-3′ and reverse primer 5′-A CCCGGG TTAATTGCCAATACTGTCATA-3′ adding Sma I and Sal I (underlined) restriction sites.

    Article Title: KEAP1 gene mutations and NRF2 activation are common in pulmonary papillary adenocarcinoma
    Article Snippet: .. Primers for PCR amplification and sequencing were designed in the area of exon 3 of KEAP1 [ ] and synthesized by Invitrogen (Carlsbad, California, United States), and amplification of DNA from early passage A549 cells (American Type Culture Collection, Manassas, Virginia, USA) and from primary tumor samples was performed using Taq polymerase (Qiagen, California, United States) as previously described [ ]. .. All potential genetic alterations were confirmed by bi-directional sequencing and testing a second, independent DNA sample from the tumor.

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: Paragraph title: Random amplified polymorphic DNA (RAPD) fingerprinting, PCR of bacterial marker genes, and sequencing of the transferred rpsL gene. ... RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ).

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen. .. Sequencing of pCM499 was carried out using primers Bam HI CW and Bam HI CCW (New England Biolabs), which anneal near the Bam HI sites of pBR322-derived plasmids.

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: The directional GS FLX Titanium adaptors A and B (including a four‐base library key sequence) were attached at the 5′ end of the primers, and a unique 12‐bp Multiplex Identifier (MID) was added between the library key and the template‐specific sequence of the forward primer to allow sequences to be assigned to samples. .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada).

    Article Title: Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence
    Article Snippet: PCR was performed using Taq polymerase (QIAGEN) or Pfu or Platinum Pfx polymerase (Stratagene) according to the manufacturers' instructions. .. All clones derived from PCR fragments were verified by sequencing.

    Article Title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon
    Article Snippet: The strain was identified by sequencing the internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA gene and internal transcribed spacer 2 (ITS2) according to White et al. ( ) and the D1/D2 domain at the 5′ end of the LSU rRNA gene according to Kurtzman and Robnett ( ). .. Twenty microlitre of PCR contained 1 µL DNA template (50 ng), 200 mM of each deoxynucleotide triphosphate, 2 µL of tenfold buffer (Taq DNA Polymerase, Qiagen, Chatsworth, CA, USA), 0.7 mM each primer, and 1.0 U Taq DNA Polymerase (Qiagen).

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: Paragraph title: CCGF sequencing ... All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.).

    Recombinant:

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: Chromosomal DNA was prepared from wild-type and recombinant bacteria using a genomic-DNA preparation kit (QIAGEN). .. RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ).

    Cellular Antioxidant Activity Assay:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. The sequences of the cytokine primers and the expected product sizes are as follows: IFN-α, 5′ primer 5′ GAC TCA TCT GCT GCT TGG AAT GCA ACC CTC C 3′ and 3′ primer 5′ GAC TCA CTC CTT CTC CTC ACT CAG TCT TGC C 3′ (294 bp); IFN-β, 5′ primer 5′ CAG CTC CAG CTC CAA GAA AGG ACG AAC ATT CG 3′ and 3′ primer 5′ CCA CCA CTC ATT CTG AGG CAT CAA CTG ACA GG 3′ (509 bp); IL-1β, 5′ primer 5′ AAG CTC TCC ACC TCA ATG GAC AG 3′ and 3′ primer 5′ CTC AAA CTC CAC TTT GCT CTT GA 3′ (260 bp); IL-6, 5′ primer 5′ CCT CTG GTC TTC TGG AGT ACC AT 3′ and 3′ primer 5′ GGC ATA ACG CAC TAG GTT TGC CG 3′ (307 bp); IL-10, 5′ primer 5′ CCA GTT TTA CCT GGT AGA AGT GAT G 3′ and 3′ primer 5′ TGT CTA GGT CCT GGA GTC CAG CAG ACT CAA 3′ (324 bp); IL-12 p40, 5′ primer 5′ ATG GCC ATG TGG GAG CTG GAG AAA G 3′ and 3′ 5′ GTG GAG CAG CAG ATG TGA GTG GCT 3′ (451 bp); IL-18, 5′ primer 5′ CTG TGT TCG AGG ATA TGA CTG 3′ and 3′ primer 5′ GTG TCC TTC ATA CAG TGA AG 3′ (283 bp); TNF-α, 5′ primer 5′ GTT CTA TGG CCC AGA CCC TCA CA 3′ and 3′ primer 5′ TAC CAG GGT TTG AGC TCA GC 3′ (364 bp); inducible no synthase (iNOS), 5′ primer 5′ TGG GAA TGG AGA CTG TCC CAG 3′ and 3′ primer 5′ GGG ATC TGA ATG TGA TGT TTG 3′ (306 bp); MIP-1α, 5′ primer 5′ ATG AAG GTC TCC ACC ACT GCC CTT G 3′ and 3′ primer 5′ GGC ATT CAG TCC AGG TCA GTG AT 3′ (276 bp); IFN-γ, 5′ primer 5′ CTT GGA TAT CTG GAG GAA CTG GC 3′ and 3′ primer 5′ GCG CTG GAC CTG TGG GTT GTT GA 3′ (271 bp); hypoxanthine phosphoribosyltransferase (HPRT), 5′ primer 5′ GTT GGA TAC AGG CCA GAC TTT GTT G 3′ and 3′ primer 5′ GAG GGT AGG CTG GCC TAT AGG CT 3′ (352 bp).

    Transmission Electron Microscopy:

    Article Title: Multiresistant Bacteria Isolated from Chicken Meat in Austria
    Article Snippet: Detection of Resistance Genes PCR detection and gene identification were carried out for three different β-lactamase gene families, bla TEM , bla SHV and bla CTX-M (including subgroup 1, 2 and 9). .. Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany) were used.

    DNA Extraction:

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: We extracted total genomic DNA from 100 to 200 mg of root material using the NucleoMag 96 Plant DNA extraction kit (Macherey‐Nagel, D‐Mark Biosciences, Toronto, ON, Canada), and from 250 to 300 mg of soil material with the PowerSoil™ DNA Isolation Kit (MOBIO Laboratories, Carlsbad, CA) according to instructions by the manufacturer. .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada).

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: Paragraph title: DNA extraction and PCR analysis ... The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA).

    Marker:

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: Paragraph title: Random amplified polymorphic DNA (RAPD) fingerprinting, PCR of bacterial marker genes, and sequencing of the transferred rpsL gene. ... RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ).

    Mutagenesis:

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ). .. Because the streptomycin resistance in H. pylori is mediated by a single point mutation (K43R) in the rpsL gene product , the rpsL gene of the recombinant progeny was sequenced using standard procedures.

    Isolation:

    Article Title: Attenuation of teratoma formation by p27 overexpression in induced pluripotent stem cells
    Article Snippet: Paragraph title: RNA isolation and reverse transcription ... Polymerase chain reaction (PCR) was performed with Taq DNA polymerase (Qiagen).

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Paragraph title: RNA isolation and reverse transcription (RT)-PCR cytokine analysis. ... Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: DNA extraction and PCR analysis Total genomic DNA was isolated from peripheral white cells using Blood Genomic DNA extraction kit following the manufacturer’s instructions (Tiangen Beijing, China). .. The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA).

    Size-exclusion Chromatography:

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada). .. An equal amount of amplified DNA from each sample was combined into a single pool and sent for pyrosequencing using Roche 454 GS FLX+ chemistry at the Genome Québec Innovation Center (McGill University, Montréal, QC, Canada).

    Purification:

    Article Title: Attenuation of teratoma formation by p27 overexpression in induced pluripotent stem cells
    Article Snippet: RNA isolation and reverse transcription Total RNA was purified using an RNeasy kit (Qiagen); 0.2 μg of total RNA was used for the reverse transcription reaction with Omniscript RT kit (Qiagen) according to the manufacturer’s instructions. .. Polymerase chain reaction (PCR) was performed with Taq DNA polymerase (Qiagen).

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: Plasmids were purified using a QiaPrep Spin plasmid kit, and DNA fragments were prepared for cloning by agarose gel extraction using a Qiaquick gel extraction kit (Qiagen). .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada). .. An equal amount of amplified DNA from each sample was combined into a single pool and sent for pyrosequencing using Roche 454 GS FLX+ chemistry at the Genome Québec Innovation Center (McGill University, Montréal, QC, Canada).

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.). .. Analysis of sequencing results Sequences obtained for ccgf analysis were analyzed by MUSCLE alignment and phylogenetic trees were drawn by neighbor-joining method using MEGA5.1 software .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Paragraph title: RNA isolation and reverse transcription (RT)-PCR cytokine analysis. ... Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA.

    Gel Extraction:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: Plasmids were purified using a QiaPrep Spin plasmid kit, and DNA fragments were prepared for cloning by agarose gel extraction using a Qiaquick gel extraction kit (Qiagen). .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    IA:

    Article Title: Iron and Fur Regulation in Vibrio cholerae and the Role of Fur in Virulence
    Article Snippet: The oligonucleotide primers for PCR were purchased from IDT Inc. (Coralville, IA). .. PCR was performed using Taq polymerase (QIAGEN) or Pfu or Platinum Pfx polymerase (Stratagene) according to the manufacturers' instructions.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. The sequences of the cytokine primers and the expected product sizes are as follows: IFN-α, 5′ primer 5′ GAC TCA TCT GCT GCT TGG AAT GCA ACC CTC C 3′ and 3′ primer 5′ GAC TCA CTC CTT CTC CTC ACT CAG TCT TGC C 3′ (294 bp); IFN-β, 5′ primer 5′ CAG CTC CAG CTC CAA GAA AGG ACG AAC ATT CG 3′ and 3′ primer 5′ CCA CCA CTC ATT CTG AGG CAT CAA CTG ACA GG 3′ (509 bp); IL-1β, 5′ primer 5′ AAG CTC TCC ACC TCA ATG GAC AG 3′ and 3′ primer 5′ CTC AAA CTC CAC TTT GCT CTT GA 3′ (260 bp); IL-6, 5′ primer 5′ CCT CTG GTC TTC TGG AGT ACC AT 3′ and 3′ primer 5′ GGC ATA ACG CAC TAG GTT TGC CG 3′ (307 bp); IL-10, 5′ primer 5′ CCA GTT TTA CCT GGT AGA AGT GAT G 3′ and 3′ primer 5′ TGT CTA GGT CCT GGA GTC CAG CAG ACT CAA 3′ (324 bp); IL-12 p40, 5′ primer 5′ ATG GCC ATG TGG GAG CTG GAG AAA G 3′ and 3′ 5′ GTG GAG CAG CAG ATG TGA GTG GCT 3′ (451 bp); IL-18, 5′ primer 5′ CTG TGT TCG AGG ATA TGA CTG 3′ and 3′ primer 5′ GTG TCC TTC ATA CAG TGA AG 3′ (283 bp); TNF-α, 5′ primer 5′ GTT CTA TGG CCC AGA CCC TCA CA 3′ and 3′ primer 5′ TAC CAG GGT TTG AGC TCA GC 3′ (364 bp); inducible no synthase (iNOS), 5′ primer 5′ TGG GAA TGG AGA CTG TCC CAG 3′ and 3′ primer 5′ GGG ATC TGA ATG TGA TGT TTG 3′ (306 bp); MIP-1α, 5′ primer 5′ ATG AAG GTC TCC ACC ACT GCC CTT G 3′ and 3′ primer 5′ GGC ATT CAG TCC AGG TCA GTG AT 3′ (276 bp); IFN-γ, 5′ primer 5′ CTT GGA TAT CTG GAG GAA CTG GC 3′ and 3′ primer 5′ GCG CTG GAC CTG TGG GTT GTT GA 3′ (271 bp); hypoxanthine phosphoribosyltransferase (HPRT), 5′ primer 5′ GTT GGA TAC AGG CCA GAC TTT GTT G 3′ and 3′ primer 5′ GAG GGT AGG CTG GCC TAT AGG CT 3′ (352 bp).

    Plasmid Preparation:

    Article Title: Properties of African Cassava Mosaic Virus Capsid Protein Expressed in Fission Yeast
    Article Snippet: .. Construction of Expression Plasmid The ACMV AV1 open reading frame (ORF) was amplified by PCR (3 min, 94 °C; 25 cycles 30 s 94 °C, 30 s 52 °C, 1 min 72 °C; 10 min 72 °C) using Taq-DNA-polymerase (Qiagen, Hilden, Germany), pUC19:APA-9 (Dr. .. Rob W. Briddon, Faisalabad, Pakistan, containing the sequence of ACMV-[Nigeria-Ogo]; AJ427910) as template, forward 5′-A CCCGGGTCGAC ATGTCGAAGCGACCAGGA-3′ and reverse primer 5′-A CCCGGG TTAATTGCCAATACTGTCATA-3′ adding Sma I and Sal I (underlined) restriction sites.

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: Plasmids were purified using a QiaPrep Spin plasmid kit, and DNA fragments were prepared for cloning by agarose gel extraction using a Qiaquick gel extraction kit (Qiagen). .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: The plasmid DNAs purified using WizardTM Plus SV Mini-preps DNA Purification System (Promega, Japan) were commercially sequenced (Operon Bio-Technology Co., Ltd. Japan) and manually analyzed using BLASTn against NCBI mouse genome database. .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.).

    Software:

    Article Title: Long‐term demographic decline and late glacial divergence in a Californian paleoendemic: Sequoiadendron giganteum (giant sequoia)
    Article Snippet: PCRs consisted of a 10 μ L solution containing 200 μ mol/L of each dNTP (Roche), 0.5 units of TAQ DNA polymerase (Qiagen, Redwood City, CA), 1 μ L −10x PCR buffer with 15 mmol/L MgCl2 , 0.4 μ mol/L reverse, 0.16 μ mol/L FAM‐labeled M13 primer, and approximately 20 ng of extracted DNA. .. Microsatellite fragments were analyzed with Genescan 3.7 and Genotyper 3.7 software (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of antibiotic pre-treatment and pathogen challenge on the intestinal microbiota in mice
    Article Snippet: .. Quantitative PCR Sets of qPCR primers (Table ) were used to quantitate bacterial populations, based on the universal bacterial 16S rRNA sequences [ ], C. jejuni luxS [ ], and A. baumannii oxa51 [ ]. qPCRs were performed using 3.5 mM MgCl2 , 0.4 ng/µl bovine serum albumin, 0.2 mM of each deoxynucleoside triphosphate, 10 pmol of each primer, 0.625 U Taq DNA polymerase (Qiagen, Valencia CA, USA), and 2 µl extracted DNA in a final 20-µl volume of SYBR green master mix. qPCR conditions included 5 min at 94 °C and 45 cycles of 10 s at 94 °C, 10 s at 60 °C (C. jejuni and A. baumannii ) or 56 °C (total bacteria). .. All assays were performed using a Light Cycler 480 (Roche Diagnostic Corporation, Indianapolis IN, USA).

    Article Title: Multiresistant Bacteria Isolated from Chicken Meat in Austria
    Article Snippet: Taq DNA polymerase and dNTPs from QIAGEN (Hilden, Germany) were used. .. The detection of the mec A genes was performed as described previously [ ] and the detection of the vancomycin resistance genes (van A/van B) was performed by real time PCR with the Light cycler VRE Detection Kit (Roche, Branchburg, New Jersey, USA) [ ].

    Multiplex Assay:

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: The directional GS FLX Titanium adaptors A and B (including a four‐base library key sequence) were attached at the 5′ end of the primers, and a unique 12‐bp Multiplex Identifier (MID) was added between the library key and the template‐specific sequence of the forward primer to allow sequences to be assigned to samples. .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada).

    Agarose Gel Electrophoresis:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: Plasmids were purified using a QiaPrep Spin plasmid kit, and DNA fragments were prepared for cloning by agarose gel extraction using a Qiaquick gel extraction kit (Qiagen). .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. PCR cycle conditions were 30 cycles of 94°C for 40 s, 60°C for 20 s, and 72°C for 40 s, followed by a final extension at 72°C for 5 min. PCR products were separated on an ethidium bromide-containing 2% agarose gel, illuminated on a UV light source, and photographed using Polaroid type 667 film.

    Article Title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon
    Article Snippet: Twenty microlitre of PCR contained 1 µL DNA template (50 ng), 200 mM of each deoxynucleotide triphosphate, 2 µL of tenfold buffer (Taq DNA Polymerase, Qiagen, Chatsworth, CA, USA), 0.7 mM each primer, and 1.0 U Taq DNA Polymerase (Qiagen). .. Meanwhile, the program for D1/D2 domain was: 95 °C, 10 min; 30 cycles: 94 °C, 30 s; 55 °C 30 s; 72 °C, 45 s; 72 °C, 7 min. A 10 µL aliquot of PCR products from each reaction, electrophoresed in TBE buffer including 2.0 % agarose gel, was stained with SYBR SAFE (Invitrogen, Eugene, OR, USA).

    Electrophoresis:

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA). .. The amplified products were analyzed by electrophoresis in 10% polyacrylamide gel (visualized by gel-red).

    Functional Assay:

    Article Title: Aspartic Peptide Hydrolases in Salmonella enterica Serovar Typhimurium
    Article Snippet: PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen. .. PCR was performed using either Taq polymerase (Qiagen) or Pfu polymerase (Stratagene) and deoxynucleoside triphosphates from Qiagen.

    Concentration Assay:

    Article Title: High richness of ectomycorrhizal fungi and low host specificity in a coastal sand dune ecosystem revealed by network analysis
    Article Snippet: .. We performed polymerase chain reaction (PCR) in triplicates using for each sample: 0.5 U of Qiagen Taq DNA Polymerase (Qiagen, Toronto, ON, Canada), 1× of the manufacturer's reaction buffer, 0.275 μ mol/L of each primer and dNTPs, a final concentration of 2.75 μ mol/L MgCl2 , and 0.83 μ L each of 1% Tween‐20, DMSO, and BSA, as well as 2 μ L of diluted DNA (1:10) in a total volume of 20 μ L. The cycling conditions were 94°C for 5 min, followed by 32 cycles of 94°C for 45 sec, 55°C for 35 sec, and 72°C for 1 min, and a final elongation of 72 °C for 7 min. Triplicates were pooled, then purified with the NucleoMag 96 PCR clean‐up kit (Macherey‐Nagel; D‐Mark Biosciences, Toronto, ON, Canada), and quantified with the Qubit® 2.0 Fluorometer (Life Technologies, Burlington, ON, Canada). .. An equal amount of amplified DNA from each sample was combined into a single pool and sent for pyrosequencing using Roche 454 GS FLX+ chemistry at the Genome Québec Innovation Center (McGill University, Montréal, QC, Canada).

    Article Title: Relationship between LAPTM4B Gene Polymorphism and Prognosis of Patients following Tumor Resection for Colorectal and Esophageal Cancers
    Article Snippet: DNA was dissolved in elution buffer, and its concentration was measured with a Nanodrop 2000 spectrophotomer (Thermo Fisher Scientific, Wilmington, Delaware, USA) and stored at -80°C until use. .. The last cycle was followed by auto-extension 72°C for 7 min with Taq DNA polymerase (Hotstar Taq plus, Qiagen, Valencia, California, USA).

    DNA Purification:

    Article Title: KEAP1 gene mutations and NRF2 activation are common in pulmonary papillary adenocarcinoma
    Article Snippet: Paragraph title: DNA Purification, amplification, and sequence analysis ... Primers for PCR amplification and sequencing were designed in the area of exon 3 of KEAP1 [ ] and synthesized by Invitrogen (Carlsbad, California, United States), and amplification of DNA from early passage A549 cells (American Type Culture Collection, Manassas, Virginia, USA) and from primary tumor samples was performed using Taq polymerase (Qiagen, California, United States) as previously described [ ].

    Article Title: Familial clustering of mice consistent to known pedigrees enabled by the genome profiling (GP) method
    Article Snippet: The plasmid DNAs purified using WizardTM Plus SV Mini-preps DNA Purification System (Promega, Japan) were commercially sequenced (Operon Bio-Technology Co., Ltd. Japan) and manually analyzed using BLASTn against NCBI mouse genome database. .. All of the 16 samples were specifically PCR amplified using PCR mix containing 200 μM dNTPs (N = G, A, T, or C), 0.5 μM primer mcF, 0.5 μM primer mcR, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , and 0.03 U/μL Taq DNA polymerase with 30 cycles of denaturation at 94°C for 30 s, annealing at 61°C for 60 s, and extension at 72°C for 60 s. PCR products were purified using PCR product purification kit (Quiagen) and commercially sequenced (Operon Bio-Technology Co., Ltd.).

    CTG Assay:

    Article Title: Direct Activation of Innate and Antigen-Presenting Functions of Microglia following Infection with Theiler's Virus
    Article Snippet: Each PCR was conducted in a 50-μl volume containing 50 mM KCl, 10 mM Tris-Cl (pH 8.3), 5 mM MgCl2 , 2 mM deoxynucleoside triphosphates, 100 pmol of each 5′ and 3′ gene-specific primer, 1 U of Taq polymerase (Qiagen, Chatsworth, Calif.), and 10 μl of diluted cDNA. .. The sequences of the cytokine primers and the expected product sizes are as follows: IFN-α, 5′ primer 5′ GAC TCA TCT GCT GCT TGG AAT GCA ACC CTC C 3′ and 3′ primer 5′ GAC TCA CTC CTT CTC CTC ACT CAG TCT TGC C 3′ (294 bp); IFN-β, 5′ primer 5′ CAG CTC CAG CTC CAA GAA AGG ACG AAC ATT CG 3′ and 3′ primer 5′ CCA CCA CTC ATT CTG AGG CAT CAA CTG ACA GG 3′ (509 bp); IL-1β, 5′ primer 5′ AAG CTC TCC ACC TCA ATG GAC AG 3′ and 3′ primer 5′ CTC AAA CTC CAC TTT GCT CTT GA 3′ (260 bp); IL-6, 5′ primer 5′ CCT CTG GTC TTC TGG AGT ACC AT 3′ and 3′ primer 5′ GGC ATA ACG CAC TAG GTT TGC CG 3′ (307 bp); IL-10, 5′ primer 5′ CCA GTT TTA CCT GGT AGA AGT GAT G 3′ and 3′ primer 5′ TGT CTA GGT CCT GGA GTC CAG CAG ACT CAA 3′ (324 bp); IL-12 p40, 5′ primer 5′ ATG GCC ATG TGG GAG CTG GAG AAA G 3′ and 3′ 5′ GTG GAG CAG CAG ATG TGA GTG GCT 3′ (451 bp); IL-18, 5′ primer 5′ CTG TGT TCG AGG ATA TGA CTG 3′ and 3′ primer 5′ GTG TCC TTC ATA CAG TGA AG 3′ (283 bp); TNF-α, 5′ primer 5′ GTT CTA TGG CCC AGA CCC TCA CA 3′ and 3′ primer 5′ TAC CAG GGT TTG AGC TCA GC 3′ (364 bp); inducible no synthase (iNOS), 5′ primer 5′ TGG GAA TGG AGA CTG TCC CAG 3′ and 3′ primer 5′ GGG ATC TGA ATG TGA TGT TTG 3′ (306 bp); MIP-1α, 5′ primer 5′ ATG AAG GTC TCC ACC ACT GCC CTT G 3′ and 3′ primer 5′ GGC ATT CAG TCC AGG TCA GTG AT 3′ (276 bp); IFN-γ, 5′ primer 5′ CTT GGA TAT CTG GAG GAA CTG GC 3′ and 3′ primer 5′ GCG CTG GAC CTG TGG GTT GTT GA 3′ (271 bp); hypoxanthine phosphoribosyltransferase (HPRT), 5′ primer 5′ GTT GGA TAC AGG CCA GAC TTT GTT G 3′ and 3′ primer 5′ GAG GGT AGG CTG GCC TAT AGG CT 3′ (352 bp).

    Staining:

    Article Title: Conjugative Transfer of Chromosomally Encoded Antibiotic Resistance from Helicobacter pylori to Campylobacter jejuni ▿
    Article Snippet: RAPD PCRs were carried out in 25-μl mixtures that contained 20 ng genomic H. pylori or C. jejuni DNA, 3 mM MgCl2 , 250 μM deoxynucleotide triphosphates, 1 unit of Taq polymerase in 1× buffer (QIAGEN), 30 pmol of the RAPD primer D9355 under cycling conditions described previously ( ). .. The PCR products were resolved in 1.0% agarose gels and visualized by staining them with ethidium bromide.

    Article Title: Isolation and identification of resveratrol-producing endophytes from wine grape Cabernet Sauvignon
    Article Snippet: Twenty microlitre of PCR contained 1 µL DNA template (50 ng), 200 mM of each deoxynucleotide triphosphate, 2 µL of tenfold buffer (Taq DNA Polymerase, Qiagen, Chatsworth, CA, USA), 0.7 mM each primer, and 1.0 U Taq DNA Polymerase (Qiagen). .. Meanwhile, the program for D1/D2 domain was: 95 °C, 10 min; 30 cycles: 94 °C, 30 s; 55 °C 30 s; 72 °C, 45 s; 72 °C, 7 min. A 10 µL aliquot of PCR products from each reaction, electrophoresed in TBE buffer including 2.0 % agarose gel, was stained with SYBR SAFE (Invitrogen, Eugene, OR, USA).

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    Qiagen hotstar plus taq polymerase
    Hotstar Plus Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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