taq gold dna polymerase  (Thermo Fisher)


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    Name:
    Taq DNA Polymerase Brasil
    Description:
    Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single stranded templates in the presence of dNTPs and a primer The enzyme consists of a single polypeptide with a molecular weight of 94 kDa It has a 5 →3 DNA polymerase activity and a 5 →3 exonuclease activity With our Taq DNA Polymerase you get • Your choice of recombinant or native enzyme• Amplification of PCR products up to 5 kb in size• An enzyme that is licensed and qualified for PCRApplicationsAmplification of DNA from complex genomic viral and plasmid templates RT PCR sequencing ssDNA and cycle sequencingSourceNative enzyme is purified from Thermus aquaticus YT1 Unit definitionOne unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C
    Catalog Number:
    11615010
    Price:
    None
    Applications:
    ChIP-on-Chip|Chromatin Immunoprecipitation (ChIP)|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Chromatin Biology
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher taq gold dna polymerase
    PCR amplification of UV-damaged <t>DNA.</t> ( A ) Human genomic DNA (K562) was damaged by exposure to UVC at a flux rate of 0.197 J/cm 2 /min. Two nanograms of damaged genomic DNA were amplified with primers specific for the Major and Precise Alu subfamilies ( 21 ). The PCR contained, inter alia, 2.5 U <t>Taq</t> Gold DNA Polymerase, 20 pmol each of the forward and reverse primers and, if applicable, 100 nM Dpo4. The forward primer was labeled at its 5′ end with the reactive fluorescent dye 6-FAM. Amplified fragments were separated by capillary electrophoresis and detected by laser-induced fluorescence of the incorporated dye-labeled primer. The electropherograms depict Alu element amplification products of the UVC damaged genomic substrate with Taq Gold DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Dpo4 ( 2 ). ( B ) Experimental details are the same as (A) except that the DNA was exposed to UVC at the same flux rate for 30 min and PCR was performed at 85°C, as noted in Materials and Methods. Results show the Alu element products obtained with AmpliTaq DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Ste ( 2 ) or Ain ( 3 ).
    Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single stranded templates in the presence of dNTPs and a primer The enzyme consists of a single polypeptide with a molecular weight of 94 kDa It has a 5 →3 DNA polymerase activity and a 5 →3 exonuclease activity With our Taq DNA Polymerase you get • Your choice of recombinant or native enzyme• Amplification of PCR products up to 5 kb in size• An enzyme that is licensed and qualified for PCRApplicationsAmplification of DNA from complex genomic viral and plasmid templates RT PCR sequencing ssDNA and cycle sequencingSourceNative enzyme is purified from Thermus aquaticus YT1 Unit definitionOne unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C
    https://www.bioz.com/result/taq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    taq gold dna polymerase - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs"

    Article Title: Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkj512

    PCR amplification of UV-damaged DNA. ( A ) Human genomic DNA (K562) was damaged by exposure to UVC at a flux rate of 0.197 J/cm 2 /min. Two nanograms of damaged genomic DNA were amplified with primers specific for the Major and Precise Alu subfamilies ( 21 ). The PCR contained, inter alia, 2.5 U Taq Gold DNA Polymerase, 20 pmol each of the forward and reverse primers and, if applicable, 100 nM Dpo4. The forward primer was labeled at its 5′ end with the reactive fluorescent dye 6-FAM. Amplified fragments were separated by capillary electrophoresis and detected by laser-induced fluorescence of the incorporated dye-labeled primer. The electropherograms depict Alu element amplification products of the UVC damaged genomic substrate with Taq Gold DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Dpo4 ( 2 ). ( B ) Experimental details are the same as (A) except that the DNA was exposed to UVC at the same flux rate for 30 min and PCR was performed at 85°C, as noted in Materials and Methods. Results show the Alu element products obtained with AmpliTaq DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Ste ( 2 ) or Ain ( 3 ).
    Figure Legend Snippet: PCR amplification of UV-damaged DNA. ( A ) Human genomic DNA (K562) was damaged by exposure to UVC at a flux rate of 0.197 J/cm 2 /min. Two nanograms of damaged genomic DNA were amplified with primers specific for the Major and Precise Alu subfamilies ( 21 ). The PCR contained, inter alia, 2.5 U Taq Gold DNA Polymerase, 20 pmol each of the forward and reverse primers and, if applicable, 100 nM Dpo4. The forward primer was labeled at its 5′ end with the reactive fluorescent dye 6-FAM. Amplified fragments were separated by capillary electrophoresis and detected by laser-induced fluorescence of the incorporated dye-labeled primer. The electropherograms depict Alu element amplification products of the UVC damaged genomic substrate with Taq Gold DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Dpo4 ( 2 ). ( B ) Experimental details are the same as (A) except that the DNA was exposed to UVC at the same flux rate for 30 min and PCR was performed at 85°C, as noted in Materials and Methods. Results show the Alu element products obtained with AmpliTaq DNA polymerase alone ( 1 ) or with a cocktail of Taq DNA polymerase and 100 nM Ste ( 2 ) or Ain ( 3 ).

    Techniques Used: Polymerase Chain Reaction, Amplification, Labeling, Electrophoresis, Fluorescence

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: 14-3-3? antagonizes FoxO to control growth, apoptosis and longevity in Drosophila
    Article Snippet: .. Real-time PCR was performed using SYBR Green and Invitrogen Taq Polymerase on a Bio-Rad MyiQ Detection System (Hercules, CA, USA). .. All reactions were done in triplicates and normalized to rp49 as internal control as well as to the expression levels of wild-type control material.

    Article Title: Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood
    Article Snippet: .. Detection by quantitative PCR For the qPCR detection, 4 µl of the extension products was transferred to a qPCR plate and mixed with 6 µl qPCR mix; 25 mM Tris–HCl, 7.5 mM magnesium chloride, 50 mM potassium chloride, 8.3 mM ammonium sulfate, 8.3% Trehalose (Acros Organics), 333 µM (each) dNTP's, 1.67 mM dithiothreitol, 833 nM of each primer (forward: 5′-TCGTGAGCCCAAGTGTTAATTTGCTTCACGA-3′ and reverse: 5′-TGCAGTCTGTAGCGAAGTTCTCATACTGCA-3′; or the hairpin primers forward: 5′-TCGTGAGCCCAAGTGTTAATTTGCTTCACGA-3′ and reverse 5′-TGCAGTCTGTAGCGAAGTTCTCATACTGCA-3′), 417 nM Molecular Beacon (FAM-CCCGCTCGCTTATGCTACCGTGACCTGCGAATCCCGAGCGGG-DABSYL, Biomers), 41.7 U/ml recombinant Taq polymerase (Fermentas) and 1.33 µM ROX reference (ROX-TTTTTTT, Biomers). .. A two-step qPCR was run with initial denaturation at 95°C for 5 min, followed by 15 s denaturation at 95°C; and 1 min annealing/extension at 60°C for 45 cycles.

    Amplification:

    Article Title: Amelogenesis Imperfecta and Screening of Mutation in Amelogenin Gene
    Article Snippet: .. All amelogenin protein coding exons sequences (exons 2, 3, 4, 5, 6, and exon 7) were amplified by Polymerase Chain Reaction (PCR) Kit (Taq DNA Polymerase, 11615-010, Invitrogen, Brazil). .. PCR amplification products were purified by use of the QIAquick PCR Purification Kit (28106, Qiagen, Germany).

    Article Title: Heliothis zea Nudivirus 1 Gene hhi1 Induces Apoptosis Which Is Blocked by the Hz-iap2 Gene and a Noncoding Gene, pag1 ▿ ▿ †
    Article Snippet: .. Briefly, amplification was carried out by adding 1 μl of cDNA to the Taq polymerase master mix (MBI Fermentas, Vilnius, Lithuania). ..

    Article Title: Microbiological Quality of Ready-to-Eat Vegetables Collected in Mexico City: Occurrence of Aerobic-Mesophilic Bacteria, Fecal Coliforms, and Potentially Pathogenic Nontuberculous Mycobacteria
    Article Snippet: .. Briefly, 3 μ L aliquots of bacterial lysates were subjected to amplification, using a standard Taq polymerase (Life Technologies, Rockville, MD) in a total volume of 50 μ L of PCR mixture; RAC1 and RAC8 and MTB-F and MTB-R primers [ ] were used for identification of the Mycobacterium genus and of the strains belonging to the M. tuberculosis complex, respectively. .. The amplicon produced by the primer combination of RAC1 and RAC8 contains the last 99 codons of the mur A gene, the promoter region of the rrn A operon, and 360 nucleotides from the 5′ end of the 16S rRNA gene.

    SYBR Green Assay:

    Article Title: 14-3-3? antagonizes FoxO to control growth, apoptosis and longevity in Drosophila
    Article Snippet: .. Real-time PCR was performed using SYBR Green and Invitrogen Taq Polymerase on a Bio-Rad MyiQ Detection System (Hercules, CA, USA). .. All reactions were done in triplicates and normalized to rp49 as internal control as well as to the expression levels of wild-type control material.

    Expressing:

    Article Title: Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
    Article Snippet: .. To verify AMP gene expression, PCR reactions were conducted with 20X diluted cDNA, 1X reaction buffer, 200 nM of each oligonucleotide, 250 uM dNTPs, 2.5 mM MgCl2 and 2.5 U of recombinant Taq polymerase (Invitrogen). .. The PCR program used was an initial step of 95 °C for 3 minutes, followed by 35 cycles of 95 °C for 10 seconds, 55 °C for 10 seconds and 72 °C for 15 seconds, finishing with 72 °C for 5 minutes.

    Polymerase Chain Reaction:

    Article Title: Amelogenesis Imperfecta and Screening of Mutation in Amelogenin Gene
    Article Snippet: .. All amelogenin protein coding exons sequences (exons 2, 3, 4, 5, 6, and exon 7) were amplified by Polymerase Chain Reaction (PCR) Kit (Taq DNA Polymerase, 11615-010, Invitrogen, Brazil). .. PCR amplification products were purified by use of the QIAquick PCR Purification Kit (28106, Qiagen, Germany).

    Article Title: Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
    Article Snippet: .. To verify AMP gene expression, PCR reactions were conducted with 20X diluted cDNA, 1X reaction buffer, 200 nM of each oligonucleotide, 250 uM dNTPs, 2.5 mM MgCl2 and 2.5 U of recombinant Taq polymerase (Invitrogen). .. The PCR program used was an initial step of 95 °C for 3 minutes, followed by 35 cycles of 95 °C for 10 seconds, 55 °C for 10 seconds and 72 °C for 15 seconds, finishing with 72 °C for 5 minutes.

    Article Title: Microbiological Quality of Ready-to-Eat Vegetables Collected in Mexico City: Occurrence of Aerobic-Mesophilic Bacteria, Fecal Coliforms, and Potentially Pathogenic Nontuberculous Mycobacteria
    Article Snippet: .. Briefly, 3 μ L aliquots of bacterial lysates were subjected to amplification, using a standard Taq polymerase (Life Technologies, Rockville, MD) in a total volume of 50 μ L of PCR mixture; RAC1 and RAC8 and MTB-F and MTB-R primers [ ] were used for identification of the Mycobacterium genus and of the strains belonging to the M. tuberculosis complex, respectively. .. The amplicon produced by the primer combination of RAC1 and RAC8 contains the last 99 codons of the mur A gene, the promoter region of the rrn A operon, and 360 nucleotides from the 5′ end of the 16S rRNA gene.

    Recombinant:

    Article Title: Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood
    Article Snippet: .. Detection by quantitative PCR For the qPCR detection, 4 µl of the extension products was transferred to a qPCR plate and mixed with 6 µl qPCR mix; 25 mM Tris–HCl, 7.5 mM magnesium chloride, 50 mM potassium chloride, 8.3 mM ammonium sulfate, 8.3% Trehalose (Acros Organics), 333 µM (each) dNTP's, 1.67 mM dithiothreitol, 833 nM of each primer (forward: 5′-TCGTGAGCCCAAGTGTTAATTTGCTTCACGA-3′ and reverse: 5′-TGCAGTCTGTAGCGAAGTTCTCATACTGCA-3′; or the hairpin primers forward: 5′-TCGTGAGCCCAAGTGTTAATTTGCTTCACGA-3′ and reverse 5′-TGCAGTCTGTAGCGAAGTTCTCATACTGCA-3′), 417 nM Molecular Beacon (FAM-CCCGCTCGCTTATGCTACCGTGACCTGCGAATCCCGAGCGGG-DABSYL, Biomers), 41.7 U/ml recombinant Taq polymerase (Fermentas) and 1.33 µM ROX reference (ROX-TTTTTTT, Biomers). .. A two-step qPCR was run with initial denaturation at 95°C for 5 min, followed by 15 s denaturation at 95°C; and 1 min annealing/extension at 60°C for 45 cycles.

    Article Title: Comparative transcriptomic analysis indicates genes associated with local and systemic resistance to Colletotrichum graminicola in maize
    Article Snippet: .. To verify AMP gene expression, PCR reactions were conducted with 20X diluted cDNA, 1X reaction buffer, 200 nM of each oligonucleotide, 250 uM dNTPs, 2.5 mM MgCl2 and 2.5 U of recombinant Taq polymerase (Invitrogen). .. The PCR program used was an initial step of 95 °C for 3 minutes, followed by 35 cycles of 95 °C for 10 seconds, 55 °C for 10 seconds and 72 °C for 15 seconds, finishing with 72 °C for 5 minutes.

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