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Bioneer Corporation taq dna polymerase
Taq Dna Polymerase, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taq dna polymerase - by Bioz Stars, 2020-04
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Related Articles

Clone Assay:

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Paragraph title: Cloning putative surfactin biosynthesis genes from the genomic library ... Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: Paragraph title: Gene cloning ... DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin.

Amplification:

Article Title: GSTM1 and GSTP1 Polymorphisms as Potential Factors for Modifying the Effect of Smoking on Inflammatory Response
Article Snippet: The 20 µL PCR reaction mixture used for GSTM1 and GSTP1 genotyping contained 10 mM of Tris-HCl (pH 9.0), 40 mM of KCl, 1.5 mM of MgCl2 , 0.25 mM of each dNTP, 1 unit of Taq polymerase (Bioneer, Seoul, Korea), 20 pmole of the forward and reverse primers, and 50 ng of genomic DNA as a template. .. Amplifications were performed using the following conditions; initial denaturation at 94℃ for 5 min, 35 cycles with denaturation at 94℃ for 1 min, annealing at 65℃ for 1 min, and extension at 72℃ for 1 min, and then completion at 72℃ for 7 min. PCR amplification of reaction mixtures was carried out using a thermal cycler, PTC-200 (MJ Research, Watertown, MA, U.S.A.).

Article Title: A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
Article Snippet: .. PCR amplification The amplification reaction mixture consisted of 10 mM Tri-HCl, 50 mM KCl, 1.5 mM MgCl2 , 200 µM of each primer, 1.5 units of Taq DNA polymerase (Bioneer, Daejeon, Korea), and 20 ng of genomic DNA as the template. .. PCR amplification was performed in an automated thermal cycler (PTC-100 Programmable Thermal Controller, MJ Research, Inc., Waltham, MA, USA).

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: .. RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures
Article Snippet: The 16S-23S rRNA regions of all mollicutes species that were used in the study were amplified using multiple primer sets under each PCR condition. .. The reactions were prepared in a volume of 25 μl that contained 2 μl of DNA, 1 U of Taq DNA polymerase, 250 μmol/l of each dNTP, 50 mmol/l of Tris-HCl (pH 8.3), 40 of mmol/l KCl, 1.5 mmol/l of MgCl2 , gel loading dye, and 5 pmol of each primer set using a PCR master mix (AccuPower PCR PreMix, Bioneer Corp., Daejeon, RO Korea).

Article Title: Comparison of Microscopy, Nested-PCR, and Real-Time-PCR Assays Using High-Throughput Screening of Pooled Samples for Diagnosis of Malaria in Asymptomatic Carriers from Areas of Endemicity in Myanmar
Article Snippet: .. The first amplification reaction used 4 μl of pooled gDNA or 2 μl of individual gDNA in a 20-μl reaction mixture (0.25 mM each dNTP, 10 mM Tris-HCl [pH 9.0], 30 mM KCl, 1.5 mM MgCl2 , and 1.0 units of Taq polymerase) containing 0.02 μM primers (P1_F and P2_R). ..

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: To clone the putative surfactin biosynthesis genes from the genomic DNA of B . subtilis 168 and B . subtilis subsp. krictiensis , we amplified the genes by PCR using primers B9 ( 5′- GCAAAATTTCCGGACAGCGGGATAT-3ʹ ) and B10 ( 5′-TCGATCCGGCCGATGTATTCGAT-3ʹ ). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul. .. PCR amplification conditions included 5 min of denaturation at 94°C; followed by 30 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 15s; and a final extension step of 72°C for 10 min. Each multiplex contained four primer pairs designed to produce amplicons sufficiently different in size and migration rate to identify the four pathogen species (B. cactivora , F. oxysporum , P. cactivora , and P. nicotianae ).

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin. .. A kanamycin-resistant colony was selected, and its plasmid was sequenced using a DNA analyzer (ABI Prism 3730xl; Perkin-Elmer, Waltham, MA, USA).

Article Title: Exposure to prenatal secondhand smoke and early neurodevelopment: Mothers and Children’s Environmental Health (MOCEH) study
Article Snippet: As a positive control, a 268-bp fragment of β-globin gene was amplified at the same time. .. PCR mixture (20 μl) for GSTM1 and GSTT1 genotyping contained 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 0.25 mM of each dNTP, 1 unit Taq polymerase (Bioneer, Seoul, Korea), 20 pmol of forward and reverse primers each, and 50–100 ng of the genomic DNA as a template.

Article Title: Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD
Article Snippet: The region that included two-point mutation at codons 416 and 420 in exon 11 (causing Glu416/Asp and Thr420/Lys substitutions) was amplified by polymerase chain reaction (PCR) using the following primers: upstream, 5′-TAATGAGCAAATGAAAGAAG-3′ and downstream, 5′-TGAGTAGATTGGAGTGCATAC-3′ to obtain a 462 bp product. .. PCR was performed in a DNA Thermal Cycler (PerkinElmer Inc., Waltham, MA, USA) in a reaction volume of 40 μL containing 100 ng DNA, 1.5 mM MgCl2 , 10 mM Tris Cl (pH 8.3), 40 mM KCl, 4% dimethyl sulfoxide, 0.2 mL of each deoxynucleoside triphosphate (dNTP) (Amersham Biosciences KK, Tokyo, Japan), 0.5 μM of each primer, and 3.75 units of Taq DNA polymerase (Bioneer, Daejeon, Korea).

Positive Control:

Article Title: Exposure to prenatal secondhand smoke and early neurodevelopment: Mothers and Children’s Environmental Health (MOCEH) study
Article Snippet: As a positive control, a 268-bp fragment of β-globin gene was amplified at the same time. .. PCR mixture (20 μl) for GSTM1 and GSTT1 genotyping contained 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 0.25 mM of each dNTP, 1 unit Taq polymerase (Bioneer, Seoul, Korea), 20 pmol of forward and reverse primers each, and 50–100 ng of the genomic DNA as a template.

Synthesized:

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: .. RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: The primers were synthesized and purified by Bioneer and the PCR was performed on an i-Cycler (Bio-Rad). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

SYBR Green Assay:

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea). .. For the quantitative PCR, SYBR Green PCR Master Mix (Applied Biosystems, Calrlsbad, CA, USA) was used and then subjected to real time PCR quantification using the ABI7300 (Applied Biosystems).

Transformation Assay:

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin. .. A kanamycin-resistant colony was selected, and its plasmid was sequenced using a DNA analyzer (ABI Prism 3730xl; Perkin-Elmer, Waltham, MA, USA).

Cell Culture:

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: The CTAB method was used to extract genomic DNA from the cultured quarantine fungi (Lee and Taylor, 1990). .. The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul.

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: Total RNA Isolation and RT-PCR Total RNA was isolated from the cultured cells using Trizol reagent (Invitrogen, Carlsbad, CA, USA). .. PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea).

Reverse Transcription Polymerase Chain Reaction:

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: .. RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: Paragraph title: Total RNA Isolation and RT-PCR ... PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea).

Sequencing:

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Cloning putative surfactin biosynthesis genes from the genomic library To obtain the surfactin biosynthesis genes from the wild-type B . subtilis genomic library, two PCR primers were designed using the sequence of the surfactin biosynthesis genes of B . subtilis 168, which was used for the Bacillus genome project and contains a surfactin synthetase gene. .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: Species-specific primer pairs, including Fo.F-Fo.R (F. oxysporum ), BC.F-BC.R (B. cactivora ), PN.F-PN.R (P. cactorum), and PC.F-PC.R (P. nicotianae ) were designed based on specific sequence data for the internal transcribed spacer (ITS) region and the Ypt1 gene in GenBank. .. The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul.

Injection:

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: Cacti genomic DNA, which was injected with the fungal pathogen, were extracted (100–200 mg frozen weight) using the Qiagen DNeasy Plant Mini Kit. .. The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul.

DNA Extraction:

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: The cloning of fbaA gene was carried out with the normally used method ( ). fbaA was amplified by PCR using genomic DNA isolated from E. coli K-12, using a genomic DNA extraction kit (GeneAll, Seoul, Korea). .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin.

Real-time Polymerase Chain Reaction:

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea). .. For the quantitative PCR, SYBR Green PCR Master Mix (Applied Biosystems, Calrlsbad, CA, USA) was used and then subjected to real time PCR quantification using the ABI7300 (Applied Biosystems).

Mutagenesis:

Article Title: Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD
Article Snippet: The region that included two-point mutation at codons 416 and 420 in exon 11 (causing Glu416/Asp and Thr420/Lys substitutions) was amplified by polymerase chain reaction (PCR) using the following primers: upstream, 5′-TAATGAGCAAATGAAAGAAG-3′ and downstream, 5′-TGAGTAGATTGGAGTGCATAC-3′ to obtain a 462 bp product. .. PCR was performed in a DNA Thermal Cycler (PerkinElmer Inc., Waltham, MA, USA) in a reaction volume of 40 μL containing 100 ng DNA, 1.5 mM MgCl2 , 10 mM Tris Cl (pH 8.3), 40 mM KCl, 4% dimethyl sulfoxide, 0.2 mL of each deoxynucleoside triphosphate (dNTP) (Amersham Biosciences KK, Tokyo, Japan), 0.5 μM of each primer, and 3.75 units of Taq DNA polymerase (Bioneer, Daejeon, Korea).

Isolation:

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: .. RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: The cloning of fbaA gene was carried out with the normally used method ( ). fbaA was amplified by PCR using genomic DNA isolated from E. coli K-12, using a genomic DNA extraction kit (GeneAll, Seoul, Korea). .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin.

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: Paragraph title: Total RNA Isolation and RT-PCR ... PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea).

Multiplex Assay:

Article Title: GSTM1 and GSTP1 Polymorphisms as Potential Factors for Modifying the Effect of Smoking on Inflammatory Response
Article Snippet: The 20 µL PCR reaction mixture used for GSTM1 and GSTP1 genotyping contained 10 mM of Tris-HCl (pH 9.0), 40 mM of KCl, 1.5 mM of MgCl2 , 0.25 mM of each dNTP, 1 unit of Taq polymerase (Bioneer, Seoul, Korea), 20 pmole of the forward and reverse primers, and 50 ng of genomic DNA as a template. .. GSTM1 and GSTP1 genotypes were determined using a multiplex PCR method.

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul. .. PCR amplification conditions included 5 min of denaturation at 94°C; followed by 30 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 15s; and a final extension step of 72°C for 10 min. Each multiplex contained four primer pairs designed to produce amplicons sufficiently different in size and migration rate to identify the four pathogen species (B. cactivora , F. oxysporum , P. cactivora , and P. nicotianae ).

Purification:

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: The primers were synthesized and purified by Bioneer and the PCR was performed on an i-Cycler (Bio-Rad). .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer).

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin. .. A kanamycin-resistant colony was selected, and its plasmid was sequenced using a DNA analyzer (ABI Prism 3730xl; Perkin-Elmer, Waltham, MA, USA).

Polymerase Chain Reaction:

Article Title: GSTM1 and GSTP1 Polymorphisms as Potential Factors for Modifying the Effect of Smoking on Inflammatory Response
Article Snippet: .. The 20 µL PCR reaction mixture used for GSTM1 and GSTP1 genotyping contained 10 mM of Tris-HCl (pH 9.0), 40 mM of KCl, 1.5 mM of MgCl2 , 0.25 mM of each dNTP, 1 unit of Taq polymerase (Bioneer, Seoul, Korea), 20 pmole of the forward and reverse primers, and 50 ng of genomic DNA as a template. .. Amplifications were performed using the following conditions; initial denaturation at 94℃ for 5 min, 35 cycles with denaturation at 94℃ for 1 min, annealing at 65℃ for 1 min, and extension at 72℃ for 1 min, and then completion at 72℃ for 7 min. PCR amplification of reaction mixtures was carried out using a thermal cycler, PTC-200 (MJ Research, Watertown, MA, U.S.A.).

Article Title: A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
Article Snippet: .. PCR amplification The amplification reaction mixture consisted of 10 mM Tri-HCl, 50 mM KCl, 1.5 mM MgCl2 , 200 µM of each primer, 1.5 units of Taq DNA polymerase (Bioneer, Daejeon, Korea), and 20 ng of genomic DNA as the template. .. PCR amplification was performed in an automated thermal cycler (PTC-100 Programmable Thermal Controller, MJ Research, Inc., Waltham, MA, USA).

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: .. RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures
Article Snippet: .. The reactions were prepared in a volume of 25 μl that contained 2 μl of DNA, 1 U of Taq DNA polymerase, 250 μmol/l of each dNTP, 50 mmol/l of Tris-HCl (pH 8.3), 40 of mmol/l KCl, 1.5 mmol/l of MgCl2 , gel loading dye, and 5 pmol of each primer set using a PCR master mix (AccuPower PCR PreMix, Bioneer Corp., Daejeon, RO Korea). .. To prevent amplicon contamination, aerosol-barrier tips were used.

Article Title: Comparison of Molecular, Microscopic, and Culture Methods for Diagnosis of Cutaneous Leishmaniasis
Article Snippet: .. First‐round reaction was carried out in 20 μl reaction mixture containing 1.5 mM MgCl2 , 0.2 mM dNTPs, 10 pmol CSB2X, 10 pmol CSB1X, 1 U Taq DNA polymerase (Bioneer), 100 pg DNA (2 μl), and 1× PCR buffer. .. The PCR conditions consisted of one initial denaturing cycle at 94°C for 5 min, followed by 30 cycles at 94°C for 30 s, 56°C for 30 s, 72°C for 40 s, and finally one cycle extension at 72°C for 5 min. Second‐round reaction was performed in 20 μl reaction mixture containing production of first‐round reaction diluted 1:2 as template and followed as described in the previous step.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: .. Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer). ..

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin. .. A kanamycin-resistant colony was selected, and its plasmid was sequenced using a DNA analyzer (ABI Prism 3730xl; Perkin-Elmer, Waltham, MA, USA).

Article Title: HOXB5 Promotes the Proliferation and Invasion of Breast Cancer Cells
Article Snippet: .. PCR was performed using Taq polymerase (Bioneer, Seongnam, Korea). .. For the quantitative PCR, SYBR Green PCR Master Mix (Applied Biosystems, Calrlsbad, CA, USA) was used and then subjected to real time PCR quantification using the ABI7300 (Applied Biosystems).

Article Title: Exposure to prenatal secondhand smoke and early neurodevelopment: Mothers and Children’s Environmental Health (MOCEH) study
Article Snippet: .. PCR mixture (20 μl) for GSTM1 and GSTT1 genotyping contained 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 0.25 mM of each dNTP, 1 unit Taq polymerase (Bioneer, Seoul, Korea), 20 pmol of forward and reverse primers each, and 50–100 ng of the genomic DNA as a template. .. The following primer sets of GSTM1 and GSTT1 genes were used for PCR reaction: 5′-GAACTCCCTGAAAAGCTAAAGC-3′ (forward) and 5′-GTTGGGCTCAAATATACGGTGG-3′ (reverse) for GSTM1, and 5′-TCACCGGATCATGGCCAGCA-3′ (forward) and 5′-TTCCTTACTGGTCCTCACATCTC-3′ (reverse) for GSTT1.

Article Title: Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD
Article Snippet: .. PCR was performed in a DNA Thermal Cycler (PerkinElmer Inc., Waltham, MA, USA) in a reaction volume of 40 μL containing 100 ng DNA, 1.5 mM MgCl2 , 10 mM Tris Cl (pH 8.3), 40 mM KCl, 4% dimethyl sulfoxide, 0.2 mL of each deoxynucleoside triphosphate (dNTP) (Amersham Biosciences KK, Tokyo, Japan), 0.5 μM of each primer, and 3.75 units of Taq DNA polymerase (Bioneer, Daejeon, Korea). .. After amplification, restriction fragment-length polymorphism analysis was performed by digesting PCR products with Hae III (Toyobo, Osaka, Japan) or Eco T14 I (Takara Bio, Otsu, Japan) at 37°C overnight.

Nested PCR:

Article Title: Comparison of Molecular, Microscopic, and Culture Methods for Diagnosis of Cutaneous Leishmaniasis
Article Snippet: Paragraph title: Nested PCR ... First‐round reaction was carried out in 20 μl reaction mixture containing 1.5 mM MgCl2 , 0.2 mM dNTPs, 10 pmol CSB2X, 10 pmol CSB1X, 1 U Taq DNA polymerase (Bioneer), 100 pg DNA (2 μl), and 1× PCR buffer.

Agarose Gel Electrophoresis:

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

Article Title: Organization and characterization of genetic regions in Bacillus subtilis subsp. krictiensis ATCC55079 associated with the biosynthesis of iturin and surfactin compounds
Article Snippet: Approximately 100 ng of genomic DNA was added to a 50 μL reaction mixture containing 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 10pmol each of primer, 250 μM dNTPs, and 1 unit of Taq polymerase (AccuPower PCR PreMix, Bioneer). .. Then, an aliquot (20 μL) of the amplification products was separated on a 1% agarose gel (SeaKem LE agarose, Lonza) in 1× TAE buffer (40 mM Tris-acetate, 1 mM EDTA, pH 8.0).

Plasmid Preparation:

Article Title: Crystallographic snapshots of active site metal shift in E. coli fructose 1,6-bisphosphate aldolase
Article Snippet: .. DNA fragments obtained by PCR amplification with Taq polymerase (Bioneer, Alameda, CA, USA) were purified using a PCR purification kit (Qiagen, Valencia, CA, USA) and ligated into the pRSF-duet-1 vector (Novagen, Madison, WI, USA), which was then transformed into E. coli ER2566 cells and plated on Luria-Bertani (LB) agar containing 0.1 mM kanamycin. .. A kanamycin-resistant colony was selected, and its plasmid was sequenced using a DNA analyzer (ABI Prism 3730xl; Perkin-Elmer, Waltham, MA, USA).

Electrophoresis:

Article Title: A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
Article Snippet: PCR amplification The amplification reaction mixture consisted of 10 mM Tri-HCl, 50 mM KCl, 1.5 mM MgCl2 , 200 µM of each primer, 1.5 units of Taq DNA polymerase (Bioneer, Daejeon, Korea), and 20 ng of genomic DNA as the template. .. Analysis of PCR products was performed by electrophoresis in ethidium bromide (0.5 µg/mL)-stained 1.7 % (w/v) agarose gels.

Article Title: Exposure to prenatal secondhand smoke and early neurodevelopment: Mothers and Children’s Environmental Health (MOCEH) study
Article Snippet: PCR mixture (20 μl) for GSTM1 and GSTT1 genotyping contained 10 mM Tris-HCl (pH 9.0), 40 mM KCl, 1.5 mM MgCl2 , 0.25 mM of each dNTP, 1 unit Taq polymerase (Bioneer, Seoul, Korea), 20 pmol of forward and reverse primers each, and 50–100 ng of the genomic DNA as a template. .. To evaluate PCR-amplified fragments, electrophoresis was performed using 3% 3:1 NuSieve/agarose gel (Cambrex Bio Science, Rockland, ME, USA).

Negative Control:

Article Title: A Simple and Efficient Multiplex PCR Assay for the Identification of Mycobacterium Genus and Mycobacterium tuberculosis Complex to the Species Level
Article Snippet: PCR amplification The amplification reaction mixture consisted of 10 mM Tri-HCl, 50 mM KCl, 1.5 mM MgCl2 , 200 µM of each primer, 1.5 units of Taq DNA polymerase (Bioneer, Daejeon, Korea), and 20 ng of genomic DNA as the template. .. The cycling parameters were 94℃ for 5 min, followed by 35 cycles of denaturation at 94℃ for 30 s, annealing at 68℃ for 30 s and a final extension at 72℃ for 7 min. Sterile distilled water was used as a negative control.

Sample Prep:

Article Title: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures
Article Snippet: The reactions were prepared in a volume of 25 μl that contained 2 μl of DNA, 1 U of Taq DNA polymerase, 250 μmol/l of each dNTP, 50 mmol/l of Tris-HCl (pH 8.3), 40 of mmol/l KCl, 1.5 mmol/l of MgCl2 , gel loading dye, and 5 pmol of each primer set using a PCR master mix (AccuPower PCR PreMix, Bioneer Corp., Daejeon, RO Korea). .. The reagent mixtures were prepared in a limited-access PCR clean room, were moved to a sample preparation room for the addition of cell lysates, and were finally moved to a PCR instrumentation room for amplification.

Migration:

Article Title: Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
Article Snippet: The following reaction mixture was used for PCR with individual primer pairs: l ul (40 ng) of diluted template genomic DNA, 1 ul 10 mM (dNTP), 2 ul of 10× Reaction buffer, 1 ul (10 pmole) primer, and 1 unit of Taq DNA polymerase (Bioneer, Korea) in a total volume of 20 ul. .. PCR amplification conditions included 5 min of denaturation at 94°C; followed by 30 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 15s; and a final extension step of 72°C for 10 min. Each multiplex contained four primer pairs designed to produce amplicons sufficiently different in size and migration rate to identify the four pathogen species (B. cactivora , F. oxysporum , P. cactivora , and P. nicotianae ).

Staining:

Article Title: NDRG2 Promotes GATA-1 Expression through Regulation of the JAK2/STAT Pathway in PMA-stimulated U937 Cells
Article Snippet: RT-PCR Total RNA from harvested cells was isolated using the TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA was synthesized from 1µg of total RNA and amplified by PCR for 35~40 cycles in a 20µl reaction mixture containing 1µl cDNA, 10 pmol of each primer, 10 mM dNTP, and 0.5 U Taq DNA polymerase (Bioneer, Daejeon, Republic of Korea). β-actin was amplified for 22 cycles and used as a loading control. .. PCR products (10µl) were electrophoresed on a 1% agarose gel and visualized by ethidium bromide staining.

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