taq dna polymerase dntpack  (Roche)


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    Structured Review

    Roche taq dna polymerase dntpack
    Taq Dna Polymerase Dntpack, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase dntpack/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase dntpack - by Bioz Stars, 2021-07
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: X-linked intellectual disability type Nascimento is a clinically distinct, probably underdiagnosed entity
    Article Snippet: .. UBE2A exons were amplified using the FastStart Taq DNA Polymerase, dNTPack (Roche) and purified with AmpureXP (Beckman Coulter, Inc) following standard protocols. .. BigDye Terminator v3.1 Cycle Sequencing Kit was used for sequencing reactions prior to sequencing on a 48-capillary 3730 DNA Analyzer (Applied Biosystems).

    Article Title: Signal transduction of MCP-1 expression induced by pancreatitis-associated ascitic fluid in pancreatic acinar cells
    Article Snippet: Total RNA (1 μg) was reversed transcribed by using 1st Strand cDNA synthesis kit (Roche, Mannheim, Germany). .. The cDNA synthesized was used as template for PCR amplification by using Taq DNA polymerase, dNTPack (Roche) in the presence of 0.2 μM MCP-1 primers (Roche): MCP-1 (sense: 5′-CACTATGCAGGTCTCTGTCACG-3′, antisense; 5′-GACTCACTTGGTTCTG-GTCCA-3′, product size: 294 bp). ..

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

    Purification:

    Article Title: X-linked intellectual disability type Nascimento is a clinically distinct, probably underdiagnosed entity
    Article Snippet: .. UBE2A exons were amplified using the FastStart Taq DNA Polymerase, dNTPack (Roche) and purified with AmpureXP (Beckman Coulter, Inc) following standard protocols. .. BigDye Terminator v3.1 Cycle Sequencing Kit was used for sequencing reactions prior to sequencing on a 48-capillary 3730 DNA Analyzer (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: Monosomy 3 Influences Epithelial-Mesenchymal Transition Gene Expression in Uveal Melanoma Patients; Consequences for Liquid Biopsy
    Article Snippet: .. The 20 µL PCR reaction mixture contained components from FastStart™ Taq DNA Polymerase, dNTPack (Roche Diagnostics, Basel, Switzerland): 0.4 µL of dNTPs, 2.0 µL of the buffer with MgCl2 , 0.1 µL of FastStart Taq DNA Polymerase, and 10 pmol/L of each primer (Generi Biotech, s.r.o., Hradec Kralove, Czech Republic). .. The 20 µL PCR reaction mixture contained components from FastStart™ Taq DNA Polymerase, dNTPack (Roche Diagnostics, Basel, Switzerland): 0.4 µL of dNTPs, 2.0 µL of the buffer with MgCl2 , 0.1 µL of FastStart Taq DNA Polymerase, and 10 pmol/L of each primer (Generi Biotech, s.r.o., Hradec Kralove, Czech Republic).

    Article Title: A novel role for SALL4 during scar-free wound healing in axolotl
    Article Snippet: .. Site-directed mutagenesis The miR-219 seed sequence was mutated by performing PCR using FastStart Taq DNA polymerase dNTPack (Roche) on the pMIR-Report luciferase-AxSall4 3′ UTR plasmid with the following primers: AxSall43′ UTR SDM For1: CTGCGCACTAGTCATCGCTGTCAGTTGAGG AxSall43′UTR SDM Rev1: AAGCATAGTCA TGGTACC CCTCTGGCCAAC AxSall43′UTR MSDM For2: GTTGGCCAGAGG GGTACCA TGACTATGCTT AxSall43′ UTR MSDM Rev2: GCTAGCGGCCGCGTGGTATCAAC The bold underlined bases are non-complementary sequence located where the miR-219 seed sequence is located. .. The two fragments were purified and combined in a PCR reaction with AxSall43′ UTR SDM For1 primer and AxSall43′ UTR MSDM Rev2 primer.

    Article Title: Gut Microbiota Markers in Obese Adolescent and Adult Patients: Age-Dependent Differential Patterns
    Article Snippet: Primers were barcoded by eight unique nucleotide sequences (Roche 454 Life Sciences, Branford, CT, United States). .. To guarantee high specificity, sensitivity and accuracy of PCR reaction, a Hi-Fi PCR Taq polymerase (FastStartTM High Fidelity PCR System, dNTPack, Roche Diagnostics, Mannheim, Germany) was employed. .. To limit the per base PCR error rates and chimeric sequences 40 cycles of PCR, 5 min of extension time, a low template concentrations (1 ng) conditions were applied ( ).

    Article Title: Signal transduction of MCP-1 expression induced by pancreatitis-associated ascitic fluid in pancreatic acinar cells
    Article Snippet: Total RNA (1 μg) was reversed transcribed by using 1st Strand cDNA synthesis kit (Roche, Mannheim, Germany). .. The cDNA synthesized was used as template for PCR amplification by using Taq DNA polymerase, dNTPack (Roche) in the presence of 0.2 μM MCP-1 primers (Roche): MCP-1 (sense: 5′-CACTATGCAGGTCTCTGTCACG-3′, antisense; 5′-GACTCACTTGGTTCTG-GTCCA-3′, product size: 294 bp). ..

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

    Mutagenesis:

    Article Title: A novel role for SALL4 during scar-free wound healing in axolotl
    Article Snippet: .. Site-directed mutagenesis The miR-219 seed sequence was mutated by performing PCR using FastStart Taq DNA polymerase dNTPack (Roche) on the pMIR-Report luciferase-AxSall4 3′ UTR plasmid with the following primers: AxSall43′ UTR SDM For1: CTGCGCACTAGTCATCGCTGTCAGTTGAGG AxSall43′UTR SDM Rev1: AAGCATAGTCA TGGTACC CCTCTGGCCAAC AxSall43′UTR MSDM For2: GTTGGCCAGAGG GGTACCA TGACTATGCTT AxSall43′ UTR MSDM Rev2: GCTAGCGGCCGCGTGGTATCAAC The bold underlined bases are non-complementary sequence located where the miR-219 seed sequence is located. .. The two fragments were purified and combined in a PCR reaction with AxSall43′ UTR SDM For1 primer and AxSall43′ UTR MSDM Rev2 primer.

    Sequencing:

    Article Title: A novel role for SALL4 during scar-free wound healing in axolotl
    Article Snippet: .. Site-directed mutagenesis The miR-219 seed sequence was mutated by performing PCR using FastStart Taq DNA polymerase dNTPack (Roche) on the pMIR-Report luciferase-AxSall4 3′ UTR plasmid with the following primers: AxSall43′ UTR SDM For1: CTGCGCACTAGTCATCGCTGTCAGTTGAGG AxSall43′UTR SDM Rev1: AAGCATAGTCA TGGTACC CCTCTGGCCAAC AxSall43′UTR MSDM For2: GTTGGCCAGAGG GGTACCA TGACTATGCTT AxSall43′ UTR MSDM Rev2: GCTAGCGGCCGCGTGGTATCAAC The bold underlined bases are non-complementary sequence located where the miR-219 seed sequence is located. .. The two fragments were purified and combined in a PCR reaction with AxSall43′ UTR SDM For1 primer and AxSall43′ UTR MSDM Rev2 primer.

    Luciferase:

    Article Title: A novel role for SALL4 during scar-free wound healing in axolotl
    Article Snippet: .. Site-directed mutagenesis The miR-219 seed sequence was mutated by performing PCR using FastStart Taq DNA polymerase dNTPack (Roche) on the pMIR-Report luciferase-AxSall4 3′ UTR plasmid with the following primers: AxSall43′ UTR SDM For1: CTGCGCACTAGTCATCGCTGTCAGTTGAGG AxSall43′UTR SDM Rev1: AAGCATAGTCA TGGTACC CCTCTGGCCAAC AxSall43′UTR MSDM For2: GTTGGCCAGAGG GGTACCA TGACTATGCTT AxSall43′ UTR MSDM Rev2: GCTAGCGGCCGCGTGGTATCAAC The bold underlined bases are non-complementary sequence located where the miR-219 seed sequence is located. .. The two fragments were purified and combined in a PCR reaction with AxSall43′ UTR SDM For1 primer and AxSall43′ UTR MSDM Rev2 primer.

    Plasmid Preparation:

    Article Title: A novel role for SALL4 during scar-free wound healing in axolotl
    Article Snippet: .. Site-directed mutagenesis The miR-219 seed sequence was mutated by performing PCR using FastStart Taq DNA polymerase dNTPack (Roche) on the pMIR-Report luciferase-AxSall4 3′ UTR plasmid with the following primers: AxSall43′ UTR SDM For1: CTGCGCACTAGTCATCGCTGTCAGTTGAGG AxSall43′UTR SDM Rev1: AAGCATAGTCA TGGTACC CCTCTGGCCAAC AxSall43′UTR MSDM For2: GTTGGCCAGAGG GGTACCA TGACTATGCTT AxSall43′ UTR MSDM Rev2: GCTAGCGGCCGCGTGGTATCAAC The bold underlined bases are non-complementary sequence located where the miR-219 seed sequence is located. .. The two fragments were purified and combined in a PCR reaction with AxSall43′ UTR SDM For1 primer and AxSall43′ UTR MSDM Rev2 primer.

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

    Synthesized:

    Article Title: Signal transduction of MCP-1 expression induced by pancreatitis-associated ascitic fluid in pancreatic acinar cells
    Article Snippet: Total RNA (1 μg) was reversed transcribed by using 1st Strand cDNA synthesis kit (Roche, Mannheim, Germany). .. The cDNA synthesized was used as template for PCR amplification by using Taq DNA polymerase, dNTPack (Roche) in the presence of 0.2 μM MCP-1 primers (Roche): MCP-1 (sense: 5′-CACTATGCAGGTCTCTGTCACG-3′, antisense; 5′-GACTCACTTGGTTCTG-GTCCA-3′, product size: 294 bp). ..

    Clone Assay:

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

    Construct:

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

    Derivative Assay:

    Article Title: Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle
    Article Snippet: Amphioxus (B. f loridae ) Transgenic Experiment The genomic DNA of Florida amphioxus (B. floridae ) was isolated by phenol-chloroform extraction from an adult individual cultured in the laboratory. .. The amphioxus Msx -CNE region was amplified from amphioxus genomic DNA by PCR with FastStart Taq DNA Polymerase, dNTPack (Roche Applied Science, Indianapolis, USA), and cloned between the HindIII site and AsiSI site of the reporter construct derived from the 72-1.27 vector containing the minimal promoter of B. floridae FoxD ( ; ). ..

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