talon xtractor buffer  (TaKaRa)

 
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    Name:
    xTractor Buffer
    Description:
    xTractor Buffer is the most flexible and efficient cell lysis buffer for protein extraction from bacterial yeast mammalian and baculovirus infected cells This cell lysis reagent provides fast and efficient 10 min lysis using mild non denaturing conditions xTractor Buffer has been optimized for extraction of his tagged proteins so it is compatible with all IMAC resins
    Catalog Number:
    635671
    Price:
    None
    Size:
    250 mL
    Category:
    xTractor cell lysis buffers Purification products Protein research
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    Structured Review

    TaKaRa talon xtractor buffer
    xTractor Buffer is the most flexible and efficient cell lysis buffer for protein extraction from bacterial yeast mammalian and baculovirus infected cells This cell lysis reagent provides fast and efficient 10 min lysis using mild non denaturing conditions xTractor Buffer has been optimized for extraction of his tagged proteins so it is compatible with all IMAC resins
    https://www.bioz.com/result/talon xtractor buffer/product/TaKaRa
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    talon xtractor buffer - by Bioz Stars, 2020-08
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Identification of novel and cross-species seroreactive proteins from Bacillus anthracis using a ligation-independent cloning-based, SOS-inducible expression system
    Article Snippet: .. After overnight expression and centrifugation, cells were resuspended and lysed using 1 mL of TALON xTractor Buffer (Clontech) supplemented with 6 M urea. .. Lysates were left shaking at room temperature for 1 hr following addition of lysis buffer.

    Protease Inhibitor:

    Article Title: Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloë festucae
    Article Snippet: .. ArcticExpress RP (DE) bacterial cells expressing the salicylate hydroxylase protein were pelleted and lysed using 108 mL xTractor Cell Lysis Buffer (Clontech Laboratories), 0.1 mg mL−1 DNaseI (Sigma-Aldrich), 200 μg mL−1 lysozyme (Sigma-Aldrich) and 1X Protease Inhibitor Cocktail (Sigma-Aldrich). .. The soluble phase was treated with 1 mL TALON Metal Affinity Resin (Clontech Laboratories) to purify the His-tagged protein following the manufacturer’s protocol.

    Incubation:

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli
    Article Snippet: .. After thawing on ice, pellets were resuspended in xTractor cell lysis buffer (Clontech Laboratories) supplemented with endonuclease (Thermo Scientific Pierce) and incubated on ice for 15 min to lyze the cells. ..

    Purification:

    Article Title: Biochemical and Proteomic Characterization of Recombinant Human α/β Hydrolase Domain 6
    Article Snippet: .. The X-Tractor buffer (Clontech, Mountain View, CA) and octyl-β-glucoside (OBG) detergent were both evaluated for purification of the hΔ29-ABHD6 following extraction from the cells, but the same co-purified proteins were presented in the eluted fractions (Supplementary Fig. ). .. Our efforts to remove the co-purified proteins by increasing the imidazole concentration in the wash buffer were unsuccessful.

    Protein Extraction:

    Article Title: Nutrient-driven O-linked N-acetylglucosamine (O-GlcNAc) cycling impacts neurodevelopmental timing and metabolism
    Article Snippet: .. Protein was extracted with Tissue Protein Extraction Reagent (T-PER) tissue protein extraction reagent (Thermo Scientific) or Xtractor buffer (Clontech). ..

    Expressing:

    Article Title: Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloë festucae
    Article Snippet: .. ArcticExpress RP (DE) bacterial cells expressing the salicylate hydroxylase protein were pelleted and lysed using 108 mL xTractor Cell Lysis Buffer (Clontech Laboratories), 0.1 mg mL−1 DNaseI (Sigma-Aldrich), 200 μg mL−1 lysozyme (Sigma-Aldrich) and 1X Protease Inhibitor Cocktail (Sigma-Aldrich). .. The soluble phase was treated with 1 mL TALON Metal Affinity Resin (Clontech Laboratories) to purify the His-tagged protein following the manufacturer’s protocol.

    Article Title: Identification of novel and cross-species seroreactive proteins from Bacillus anthracis using a ligation-independent cloning-based, SOS-inducible expression system
    Article Snippet: .. After overnight expression and centrifugation, cells were resuspended and lysed using 1 mL of TALON xTractor Buffer (Clontech) supplemented with 6 M urea. .. Lysates were left shaking at room temperature for 1 hr following addition of lysis buffer.

    Article Title: Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit
    Article Snippet: .. Cells expressing the His-Rps26 fusion protein were lysed in Talon Xtractor Buffer (Clontech Laboratories, Inc.). .. The lysate was centrifuged to remove cell debris, and the supernatant was incubated with purified yeast FLAG-Upf1 for 1 h at 4°C.

    Lysis:

    Article Title: Intrinsic Structural Features of the Human IRE1α Transmembrane Domain Sense Membrane Lipid Saturation
    Article Snippet: .. The harvested cells were lysed using the xTractor cell lysis buffer (Clontech) containing 0.2 mg/mL lysozyme. .. The CAT concentration was quantified with the CAT enzyme-linked immunosorbent assay kit (Roche Applied Science) according to the product manual.

    Article Title: Functional characterization of salicylate hydroxylase from the fungal endophyte Epichloë festucae
    Article Snippet: .. ArcticExpress RP (DE) bacterial cells expressing the salicylate hydroxylase protein were pelleted and lysed using 108 mL xTractor Cell Lysis Buffer (Clontech Laboratories), 0.1 mg mL−1 DNaseI (Sigma-Aldrich), 200 μg mL−1 lysozyme (Sigma-Aldrich) and 1X Protease Inhibitor Cocktail (Sigma-Aldrich). .. The soluble phase was treated with 1 mL TALON Metal Affinity Resin (Clontech Laboratories) to purify the His-tagged protein following the manufacturer’s protocol.

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli
    Article Snippet: .. After thawing on ice, pellets were resuspended in xTractor cell lysis buffer (Clontech Laboratories) supplemented with endonuclease (Thermo Scientific Pierce) and incubated on ice for 15 min to lyze the cells. ..

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  • Team
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  • 95
    TaKaRa talon xtractor buffer
    Talon Xtractor Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/talon xtractor buffer/product/TaKaRa
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    talon xtractor buffer - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

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