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Eppendorf AG tabletop centrifuge
Tabletop Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 98/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tabletop centrifuge/product/Eppendorf AG
Average 98 stars, based on 12 article reviews
Price from $9.99 to $1999.99
tabletop centrifuge - by Bioz Stars, 2020-04
98/100 stars

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Centrifugation:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: Large oligomers formed by Drp1 samples, in the presence of ligands, were found in the pellet after a medium speed centrifugation. .. The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf).

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: .. After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature. ..

Article Title: Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Article Snippet: .. The assembled fixture was then put into a tabletop centrifuge (5810R, Eppendorf AG, Hamburg, Germany) and centrifuged at a speed of 800 rpm for 5 min. As both surfaces between the reagent chip and the stock block were superhydrophobic, no leakage was found during the centrifugation process. .. After that, the reagent chip with the dispensed reagents in microwells was turned upside down and aligned to the cell chip for the delivery of reagents to cells using both hands with naked eyes or a custom-made microaligner with a stereoscope.

Article Title: Contrasting effects of copper limitation on the photosynthetic apparatus in two strains of the open ocean diatom Thalassiosira oceanica
Article Snippet: .. The samples were then settled by10 seconds centrifugation in a tabletop centrifuge and the supernatant was transferred to a new 2 mL Eppendorf tube. .. The remaining cell debris and beads were repeatedly washed with lysis buffer and the supernatants were combined until the wash steps resulted in an almost clear supernatant (approximately 5–10 times).

Luciferase:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: Paragraph title: Luciferase reporter gene assay for cell-cell membrane fusion. ... After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature.

Autoradiography:

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min. .. Gels were dried and subjected to autoradiography and scanned using the Fujifilm FLA-9000 phosphorimager for visualization of radioactively labeled translated proteins.

Construct:

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: For ADR1a constructs, the translation reactions also included either 50 μM zinc acetate or 50 μM of the Zn2+ chelator TPEN. .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Incubation:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: Specifically, protein was diluted in PBS buffer to 0.05 mg/ml (0.62 µM), and specified WT and ∆VD mutant samples were incubated at room temperature with lipid nanotubes (200 µM) and/or GMPPCP (1 mM) for at least 30 minutes. .. The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf).

Article Title: Search for a Prion-Specific Nucleic Acid
Article Snippet: .. Typically, 300-μl samples were incubated for 16 h at 37°C on a rocking platform and centrifuged at 14,000 × g in a tabletop centrifuge (Eppendorf, Hamburg, Germany) for 30 min at room temperature. ..

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: .. After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature. ..

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min. .. The samples were resolved on 12% Bis-Tris gels (Thermo Scientific) in MOPS buffer for ADR1 and MES buffer for Spectrin and Titin.

Article Title: Association of SWAP-70 with the B cell antigen receptor complex
Article Snippet: The cell pellet was resuspended in 400 μl of hypotonic buffer [10 mM Hepes (pH 7.5)/5 mM KCl/2 mM EDTA/2 mM DTT/1 mM Na3 VO4 /protease inhibitors) and incubated on ice for 15 min to allow cells to swell. .. The suspension was spun at 6,000 rpm in an Eppendorf 5415 C tabletop centrifuge for 5 min. Pelleted nuclei were washed twice in hypotonic buffer containing 0.5% Nonidet P-40 to lyse unbroken cells.

Activity Assay:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature. .. The luciferase activity resulting from fusion of the two cell populations was quantified with a Veritas luminometer (Promega); 100 μl luciferase assay substrate (Promega) was injected into each sample.

Expressing:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: Soybean trypsin inhibitor (20 μg/ml; Sigma) was then added, and the samples were incubated at 37°C for 30 min. BSR-T7/5 target cells (expressing T7 RNA polymerase) were overlaid onto the Vero cells expressing the SeV F and HN proteins. .. After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature.

Modification:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY). .. Briefly, genomic DNA was isolated and labeled as described previously with a modification ( , ).

Western Blot:

Article Title: Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
Article Snippet: .. Single channel pipettes (Gilson, models: P2G, P20G, P200G, P1000G) Orbital shaker (Thermo Fisher Scientific, model: MaxQ™ 2000) 20 °C incubator (Winecooler, LIEBHERR, model: WK 4126) Stereo microscope (Leica, model: MZ7.5) Pellet pestle (motor) (Sigma-Aldrich, catalog number: Z359971) Tabletop centrifuge, cooled (Eppendorf, model: 5424 R) Centrifuge (Thermo Fisher Scientific, model: SL 40R) Autoclave (VWR, model: VAPOUR-Line Lite) -80 °C freezer (Sanyo, model: VIP plus) Mini PROTEAN 3 system (Bio-Rad Laboratories, catalog number: 1658001edu) WB Imager (GE Healthcare, model: ImageQuant LAS 4000 mini, catalog number: 28955813) .. ) Microsoft Excel (Microsoft Corporation, Redmond, USA)

Hybridization:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: Paragraph title: DNA isolation, labeling, and hybridization. ... A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY).

High Performance Liquid Chromatography:

Article Title: Expressed Genome of Methylobacillus flagellatus as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ †
Article Snippet: Paragraph title: Cultivation, sample preparation, and high-performance liquid chromatography (HPLC) prefractionation. ... Suspensions after the first two washes were centrifuged for 5 min at 8,500 rpm in a tabletop centrifuge (Eppendorf, Westbury, NY), and the supernatant was collected.

Transfection:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: Briefly, six-well dishes containing Vero cells (70% to 80% confluence) were transfected with 1.0 μg luciferase control DNA (Promega), 1.0 μg pCAGGS F DNA, and 1.0 μg pCAGGS HN DNA. .. After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature.

Protease Inhibitor:

Article Title: Contrasting effects of copper limitation on the photosynthetic apparatus in two strains of the open ocean diatom Thalassiosira oceanica
Article Snippet: To disrupt the cells, approximately 0.8 g of glass beads (212–300 μm diameter, Sigma, #G1277-100g) were added to the cell pellet in the Eppendorf tube, as well as ~ 1.5 mL lysis buffer (0.05 M Hepes, 0.02 M KCl, 0.001 M EDTA, 0.0002 M DTT, 0.15 Sorbitol plus freshly added Protease Inhibitor, one Roche® Tablet per 50 mL lysis buffer). .. The samples were then settled by10 seconds centrifugation in a tabletop centrifuge and the supernatant was transferred to a new 2 mL Eppendorf tube.

Transferring:

Article Title: Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Article Snippet: This pipette can adjust the tip spacing from 9 mm to 4.5 mm, which corresponds to the well spacing from a 96- to a 384-plate. .. The assembled fixture was then put into a tabletop centrifuge (5810R, Eppendorf AG, Hamburg, Germany) and centrifuged at a speed of 800 rpm for 5 min. As both surfaces between the reagent chip and the stock block were superhydrophobic, no leakage was found during the centrifugation process.

Sedimentation:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: Paragraph title: Sedimentation Assay ... The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf).

Generated:

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: In vitro transcription and translation The generated constructs were translated for 20 min. in the PURExpress Δ-Ribosome kit supplemented with high-salt-washed ribosomes isolated from HDB140, HDB143 (uL23 Δloop), or HDB144 (uL24 Δloop). .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Protein Concentration:

Article Title: SNAREs are concentrated in cholesterol-dependent clusters that define docking and fusion sites for exocytosis
Article Snippet: PC12 cells grown on 3-cm dishes were washed twice in ice-cold PBS, scraped and spun down at 2000 r.p.m. at 4°C in a tabletop centrifuge (Eppendorf). .. The cells were subsequently lysed at 4°C at a protein concentration of 1.2 mg/ml in 200 µl TNE (25 mM Tris–HCl pH 7.4, 150 mM NaCl, 5 mM EDTA, 1 mM DTT, 1 mg/ml antipain, 1 mg/ml leupeptin, and 1 mM PMSF), 10% sucrose, 1% Triton X-100.

Polymerase Chain Reaction:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY). .. Genomic DNA was digested completely with EcoRV (New England Biolabs, Beverly, MA) and purified by using the QIAquick PCR purification kit (QIAGEN, Valencia, CA).

Injection:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature. .. The luciferase activity resulting from fusion of the two cell populations was quantified with a Veritas luminometer (Promega); 100 μl luciferase assay substrate (Promega) was injected into each sample.

Reporter Gene Assay:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: Paragraph title: Luciferase reporter gene assay for cell-cell membrane fusion. ... After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature.

DNA Extraction:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: Paragraph title: DNA isolation, labeling, and hybridization. ... A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY).

Mutagenesis:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: Specifically, protein was diluted in PBS buffer to 0.05 mg/ml (0.62 µM), and specified WT and ∆VD mutant samples were incubated at room temperature with lipid nanotubes (200 µM) and/or GMPPCP (1 mM) for at least 30 minutes. .. The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf).

Isolation:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY). .. Briefly, genomic DNA was isolated and labeled as described previously with a modification ( , ).

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: In vitro transcription and translation The generated constructs were translated for 20 min. in the PURExpress Δ-Ribosome kit supplemented with high-salt-washed ribosomes isolated from HDB140, HDB143 (uL23 Δloop), or HDB144 (uL24 Δloop). .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Clarification Assay:

Article Title: Search for a Prion-Specific Nucleic Acid
Article Snippet: Briefly, brains were homogenized by three 15-s strokes to contain final 10% (wt/vol) followed by a clarification spin at 500 × g for 5 min. .. Typically, 300-μl samples were incubated for 16 h at 37°C on a rocking platform and centrifuged at 14,000 × g in a tabletop centrifuge (Eppendorf, Hamburg, Germany) for 30 min at room temperature.

Labeling:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: Paragraph title: DNA isolation, labeling, and hybridization. ... A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY).

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min. .. Gels were dried and subjected to autoradiography and scanned using the Fujifilm FLA-9000 phosphorimager for visualization of radioactively labeled translated proteins.

Purification:

Article Title: Genomic Comparison of Escherichia coli K1 Strains Isolated from the Cerebrospinal Fluid of Patients with Meningitis †
Article Snippet: A 1.5-ml amount of overnight culture was centrifuged for 5 min at 4°C at high speed in a tabletop centrifuge (Eppendorf, Westbury, NY). .. Genomic DNA was digested completely with EcoRV (New England Biolabs, Beverly, MA) and purified by using the QIAquick PCR purification kit (QIAGEN, Valencia, CA).

Article Title: Quantitative evaluation of statistical errors in small-angle X-ray scattering measurements
Article Snippet: Samples for SAXS measurements Cytochrome c , lysozyme and bovine serum albuminum (BSA) were purchased from Sigma–Aldrich and applied without further purification. .. Prior to the measurements, both buffer and sample solutions were filtered through 0.22 µm syringe filters (Thermo Scientific, USA) and centrifuged at 13 500 r min−1 for 10 min in a tabletop centrifuge (Eppendorf, Germany).

Protein Purification:

Article Title: Contrasting effects of copper limitation on the photosynthetic apparatus in two strains of the open ocean diatom Thalassiosira oceanica
Article Snippet: Paragraph title: Protein purification ... The samples were then settled by10 seconds centrifugation in a tabletop centrifuge and the supernatant was transferred to a new 2 mL Eppendorf tube.

Blocking Assay:

Article Title: Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Article Snippet: .. The assembled fixture was then put into a tabletop centrifuge (5810R, Eppendorf AG, Hamburg, Germany) and centrifuged at a speed of 800 rpm for 5 min. As both surfaces between the reagent chip and the stock block were superhydrophobic, no leakage was found during the centrifugation process. .. After that, the reagent chip with the dispensed reagents in microwells was turned upside down and aligned to the cell chip for the delivery of reagents to cells using both hands with naked eyes or a custom-made microaligner with a stereoscope.

Lysis:

Article Title: Spring-Loaded Heptad Repeat Residues Regulate the Expression and Activation of Paramyxovirus Fusion Protein ▿
Article Snippet: .. After an 8-h incubation at 37°C, the monolayers were washed, lysed in reporter lysis buffer (Promega), and clarified by centrifugation at 15,000 × g in a tabletop centrifuge (5417C; Eppendorf) at room temperature. ..

Article Title: Expressed Genome of Methylobacillus flagellatus as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ †
Article Snippet: Lysis was carried out as described previously , except that the resuspension buffer was 15 mM Tris HCl (pH 8.5)-5 mM dithiothreitol-0.01% Rapigest (Waters, Milford, MA) and the boiling time was 5 min. Digestion was carried out as described previously , except that after alkylation, a second reduction was carried out with 10 mM dithiothreitol for 30 min at 37°C and the pellet wash protocol was 250 μl of the following: once with 0.5% trifluoroacetic acid (TFA) and 5% acetonitrile, once with 0.5% TFA and 25% acetonitrile, and once with 0.5% TFA and 50% acetonitrile. .. Suspensions after the first two washes were centrifuged for 5 min at 8,500 rpm in a tabletop centrifuge (Eppendorf, Westbury, NY), and the supernatant was collected.

Article Title: Contrasting effects of copper limitation on the photosynthetic apparatus in two strains of the open ocean diatom Thalassiosira oceanica
Article Snippet: Complete cell lysis was achieved with three 1 min vortexing intervals, interrupted by 1 min cooling periods on ice. .. The samples were then settled by10 seconds centrifugation in a tabletop centrifuge and the supernatant was transferred to a new 2 mL Eppendorf tube.

Chromatin Immunoprecipitation:

Article Title: Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Article Snippet: .. The assembled fixture was then put into a tabletop centrifuge (5810R, Eppendorf AG, Hamburg, Germany) and centrifuged at a speed of 800 rpm for 5 min. As both surfaces between the reagent chip and the stock block were superhydrophobic, no leakage was found during the centrifugation process. .. After that, the reagent chip with the dispensed reagents in microwells was turned upside down and aligned to the cell chip for the delivery of reagents to cells using both hands with naked eyes or a custom-made microaligner with a stereoscope.

SDS Page:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf). .. The supernatant and pellet fractions were separated, collected, and immediately mixed with SDS-PAGE loading dye (Bio-Rad) and heated briefly at 100 °C.

Plasmid Preparation:

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: Plasmid DNA of each construct (300 ng) was used as a template for polypeptide synthesis, and translation was carried out in the presence of (35 S) Methionine at 37°C for 20 min and shaking at 500 r.p.m. .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Software:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf). .. Gels were scanned (HP Scanjet 8300) and densitometry analysis was done using the ImageJ software .

Microscopy:

Article Title: Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans
Article Snippet: .. Single channel pipettes (Gilson, models: P2G, P20G, P200G, P1000G) Orbital shaker (Thermo Fisher Scientific, model: MaxQ™ 2000) 20 °C incubator (Winecooler, LIEBHERR, model: WK 4126) Stereo microscope (Leica, model: MZ7.5) Pellet pestle (motor) (Sigma-Aldrich, catalog number: Z359971) Tabletop centrifuge, cooled (Eppendorf, model: 5424 R) Centrifuge (Thermo Fisher Scientific, model: SL 40R) Autoclave (VWR, model: VAPOUR-Line Lite) -80 °C freezer (Sanyo, model: VIP plus) Mini PROTEAN 3 system (Bio-Rad Laboratories, catalog number: 1658001edu) WB Imager (GE Healthcare, model: ImageQuant LAS 4000 mini, catalog number: 28955813) .. ) Microsoft Excel (Microsoft Corporation, Redmond, USA)

Article Title: Association of SWAP-70 with the B cell antigen receptor complex
Article Snippet: Cells were opened by passing the suspension several times through a 25-gauge needle; cell breakage was monitored under the microscope. .. The suspension was spun at 6,000 rpm in an Eppendorf 5415 C tabletop centrifuge for 5 min. Pelleted nuclei were washed twice in hypotonic buffer containing 0.5% Nonidet P-40 to lyse unbroken cells.

Sample Prep:

Article Title: Expressed Genome of Methylobacillus flagellatus as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ as Defined through Comprehensive Proteomics and New Insights into Methylotrophy ▿ †
Article Snippet: Paragraph title: Cultivation, sample preparation, and high-performance liquid chromatography (HPLC) prefractionation. ... Suspensions after the first two washes were centrifuged for 5 min at 8,500 rpm in a tabletop centrifuge (Eppendorf, Westbury, NY), and the supernatant was collected.

In Vitro:

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: Paragraph title: In vitro transcription and translation ... The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Evaporation:

Article Title: Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays
Article Snippet: The assembled fixture was then put into a tabletop centrifuge (5810R, Eppendorf AG, Hamburg, Germany) and centrifuged at a speed of 800 rpm for 5 min. As both surfaces between the reagent chip and the stock block were superhydrophobic, no leakage was found during the centrifugation process. .. This alignment procedure was done in a custom-built glovebox with a controlled humidity to prevent the evaporation of droplets.

Concentration Assay:

Article Title: Search for a Prion-Specific Nucleic Acid
Article Snippet: Samples were mixed with stock solution containing 4% NaPTA and 170 mM MgCl2 , pH 7.4, to obtain a final concentration of 0.2 to 0.3% NaPTA ( , , ). .. Typically, 300-μl samples were incubated for 16 h at 37°C on a rocking platform and centrifuged at 14,000 × g in a tabletop centrifuge (Eppendorf, Hamburg, Germany) for 30 min at room temperature.

Article Title: The shape of the bacterial ribosome exit tunnel affects cotranslational protein folding
Article Snippet: Translation was stopped by treating the sample with a final concentration of 5% trichloroacetic acid (TCA) and incubated on ice for 30 min. .. The TCA precipitated samples were subsequently centrifuged at 20,000 g for 10 min in a tabletop centrifuge (Eppendorf) and the pellet obtained was solubilized in sample buffer, supplemented with RNaseA (400 μg/ml), and incubated at 37°C for 15 min.

Fractionation:

Article Title: Association of SWAP-70 with the B cell antigen receptor complex
Article Snippet: Paragraph title: Subcellular Fractionation. ... The suspension was spun at 6,000 rpm in an Eppendorf 5415 C tabletop centrifuge for 5 min. Pelleted nuclei were washed twice in hypotonic buffer containing 0.5% Nonidet P-40 to lyse unbroken cells.

Staining:

Article Title: Cryo-EM Studies of Drp1 Reveal Cardiolipin Interactions that Activate the Helical Oligomer
Article Snippet: The mixtures were then spun at 13,200 rpm (16,100 × g ) for 30 min in a tabletop centrifuge (Eppendorf). .. These samples were run on an SDS-PAGE gel and stained with an InstantBlue Coomassie dye (Expedeon).

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    Eppendorf AG tabletop microcentrifuge
    Characterization of chromatin digestion efficiency with different MNase concentrations. (a) Electropherogram of digested chromosomal DNA collected from cells processed in the droplet microfluidic devices (on-chip, lane 1-4) and 1.5-mL <t>microcentrifuge</t> tubes (off-chip, lane 5-8). MNase concentrations are listed on the bottom. (b) Electrophoretic band intensity profiles of on-chip samples. The peaks at 35 bp and 10380 bp are internal standards. (c) Mononucleosome yields from all samples. Error bars are standard deviation of n=3 replicates.
    Tabletop Microcentrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tabletop microcentrifuge/product/Eppendorf AG
    Average 80 stars, based on 1 article reviews
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    tabletop microcentrifuge - by Bioz Stars, 2020-04
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    95
    Eppendorf AG eppendorf tabletop centrifuge
    Characterization of chromatin digestion efficiency with different MNase concentrations. (a) Electropherogram of digested chromosomal DNA collected from cells processed in the droplet microfluidic devices (on-chip, lane 1-4) and 1.5-mL <t>microcentrifuge</t> tubes (off-chip, lane 5-8). MNase concentrations are listed on the bottom. (b) Electrophoretic band intensity profiles of on-chip samples. The peaks at 35 bp and 10380 bp are internal standards. (c) Mononucleosome yields from all samples. Error bars are standard deviation of n=3 replicates.
    Eppendorf Tabletop Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eppendorf tabletop centrifuge/product/Eppendorf AG
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eppendorf tabletop centrifuge - by Bioz Stars, 2020-04
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    99
    Eppendorf AG minispin tabletop centrifuge
    Characterization of chromatin digestion efficiency with different MNase concentrations. (a) Electropherogram of digested chromosomal DNA collected from cells processed in the droplet microfluidic devices (on-chip, lane 1-4) and 1.5-mL <t>microcentrifuge</t> tubes (off-chip, lane 5-8). MNase concentrations are listed on the bottom. (b) Electrophoretic band intensity profiles of on-chip samples. The peaks at 35 bp and 10380 bp are internal standards. (c) Mononucleosome yields from all samples. Error bars are standard deviation of n=3 replicates.
    Minispin Tabletop Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minispin tabletop centrifuge/product/Eppendorf AG
    Average 99 stars, based on 1 article reviews
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    Characterization of chromatin digestion efficiency with different MNase concentrations. (a) Electropherogram of digested chromosomal DNA collected from cells processed in the droplet microfluidic devices (on-chip, lane 1-4) and 1.5-mL microcentrifuge tubes (off-chip, lane 5-8). MNase concentrations are listed on the bottom. (b) Electrophoretic band intensity profiles of on-chip samples. The peaks at 35 bp and 10380 bp are internal standards. (c) Mononucleosome yields from all samples. Error bars are standard deviation of n=3 replicates.

    Journal: Lab on a chip

    Article Title: A Droplet Microfluidic Platform for Efficient Enzymatic Chromatin Digestion Enables Robust Determination of Nucleosome Positioning

    doi: 10.1039/c8lc00599k

    Figure Lengend Snippet: Characterization of chromatin digestion efficiency with different MNase concentrations. (a) Electropherogram of digested chromosomal DNA collected from cells processed in the droplet microfluidic devices (on-chip, lane 1-4) and 1.5-mL microcentrifuge tubes (off-chip, lane 5-8). MNase concentrations are listed on the bottom. (b) Electrophoretic band intensity profiles of on-chip samples. The peaks at 35 bp and 10380 bp are internal standards. (c) Mononucleosome yields from all samples. Error bars are standard deviation of n=3 replicates.

    Article Snippet: Aqueous and oil phases were separated by centrifugation at 8000 rpm for 1 min at room temperature in a tabletop microcentrifuge (Centrifuge 5418, Eppendorf, Hauppauge, NY).

    Techniques: Chromatin Immunoprecipitation, Standard Deviation